Non-Isobaric Triplex-Labeling Strategies for Proteomics (2007)
Schweiger-Hufnagel, U., Behrens, M., Hahner, S., Keidel, E., Dosch, D., Martin, S., ...
A strategy for phosphoproteomics that integrates various gel- and MS-based techniques (2005)
Colby, T, Hufnagel, P, Lubeck, M, Suckau, D, Baessmann, C, Schmidt, J, ...
Automatic measurement and interpretation of MALDI-PSD mass spectra (1999)
R. Frey, A. Holle, A. Resemann, K. O. Kruter, R. Paape, D. Suckau
ometer with reflector, Bruker REFLEX III (Bruker Daltonik GmbH, Bremen). The standard automation (AutoXecute) and PSD (FAST) software package has been extended by a module for automatic Peptide...
Coexisting stable conformations of gaseous protein ions.
Suckau, D, Shi, Y, Beu, S C, Senko, M W, Quinn, J P, Wampler, F M, ...
For further insight into the role of solvent in protein conformer stabilization, the structural and dynamic properties of protein ions in vacuo have been probed by hydrogen-deuterium exchange in a...
Suckau, D, Mak, M, Przybylski, M
Aminoacetylation of lysine residues and the modification of arginine by 1,2-cyclohexanedione to N7,N8-(dihydroxy-1,2-cyclohexylidene)arginine were used for probing the surface topology of...
Suckau, D, Köhl, J, Karwath, G, Schneider, K, Casaretto, M, Bitter-Suermann, D, ...
Sequences of antigenic determinants were identified by limited proteolysis of peptide antigens bound to an immobilized monoclonal antibody and direct molecular weight determination of the monoclonal...
Gauss, C, Klein, J, Post, K, Suckau, D, Schneider, K, Thomas, H, ...
The direct molecular weight determination and structural analysis of polypeptides and peptide mixtures have become amenable by the recent development of fast atom bombardment (FABMS) and 252Cf-plasma...
Coexisting stable conformations of gaseous protein ions.
Suckau, D, Shi, Y, Beu, S C, Senko, M W, Quinn, J P, Wampler, F M, ...
For further insight into the role of solvent in protein conformer stabilization, the structural and dynamic properties of protein ions in vacuo have been probed by hydrogen-deuterium exchange in a...
Suckau, D, Mak, M, Przybylski, M
Aminoacetylation of lysine residues and the modification of arginine by 1,2-cyclohexanedione to N7,N8-(dihydroxy-1,2-cyclohexylidene)arginine were used for probing the surface topology of...
Suckau, D, Köhl, J, Karwath, G, Schneider, K, Casaretto, M, Bitter-Suermann, D, ...
Sequences of antigenic determinants were identified by limited proteolysis of peptide antigens bound to an immobilized monoclonal antibody and direct molecular weight determination of the monoclonal...
Gauss, C, Klein, J, Post, K, Suckau, D, Schneider, K, Thomas, H, ...
The direct molecular weight determination and structural analysis of polypeptides and peptide mixtures have become amenable by the recent development of fast atom bombardment (FABMS) and 252Cf-plasma...
P209-M Non-Isobaric Triplex-Labeling Strategies for Proteomics
Schweiger-Hufnagel, U., Behrens, M., Hahner, S., Keidel, E., Dosch, D., Martin, S., ...
A non-isobaric label chemistry is introduced that allows the triplexed quantification of proteomic samples based on the isotope-coded protein-labeling technology (ICPL). Amino groups of intact...
Keidel, E., Brunner, A., Halder, T., Suckau, D., Martin, S., Kellermann, J., ...
Already before the occurrence of pathological symptoms in the development of severe diseases changes in the proteome pattern in the plasma of patients can be observed. Therefore, to follow these...
P145-S Interactive and Automated Top-Down Analysis of Sequencing Data of Intact Proteins
Suckau, D., Vorwerg, L., Resemann, A., Witt, M., Easterling, M.
In a Top-Down workflow (ECD, ETD, ISD) the whole protein molecule is subjected to fragmentation and proteins are not digested (as in the Bottom-Up approach). This allows the detection of signal...
Sparbier, K., Asperger, A., Resemann, A., Kessler, I., Koch, S., Wenzel, T., ...
Comprehensive proteomic analyses require efficient and selective pre-fractionation to facilitate analysis of post-translationally modified peptides and proteins and automated analysis procedures for...
P41-M Automated LC-MALDI Analysis of Glycopeptides from Glycoprotein Digests Using DHB as Matrix
Resemann, A., Asperger, A., Sparbier, K., Seemann, K., Eichhorn, T., Hunzinger, C., ...
2,5-Dihydroxybenzoic acid (DHB) is the matrix of choice for carbohydrate and glycopeptide analysis, but due to the inhomogeneous surface morphology of samples prepared with DHB, it is typically...
P110-M A New Preparation Technique for MALDI Tissue Imaging
Schuerenberg, M., Luebbert, C., Deininger, S., Mueller, R., Suckau, D.
The quality of MALDI Imaging analyses crucially depends on the matrix application protocols. Unfortunately, the currently used protocols have significant disadvantages that impair the robust and...
P203-M A Software Platform for the Validation of Quantitative Proteomics Data
Jabs, W., Hahner, S., Schweiger-Hufnagel, U., Behrens, M., Lubeck, M., Asperger, A., ...
Methods based on isotope labeling and LC-MS/MS analysis are becoming a standard approach for the simultaneous identification and quantification of proteins on the proteomic scale, as they provide...
P201-T ICPL Duplex and Triplex Technology for Quantitative Proteomics
Hahner, S., Jabs, W., Brand, S., Dikler, S., Gounder, G., Niehaus, K., ...
The isotope-coded protein label (ICPL) technology has shown to be efficient for comparative quantification of proteins. A major aspect of this chemistry is the labeling of all free amino groups on...
P98-M Class Imaging—Multivariate Statistical Classification of MALDI Tissue Imaging Data
Deininger, S., Schuerenberg, M., Luebbert, C., Fuetterer, A., Gerhard, M., Suckau, D., ...
MALDI imaging is a powerful tool that allows the acquisition of tissue-specific mass spectra with spatial resolution. Here we applied statistical tools to generate information from MALDI imaging...
Hufnagel, P., Glandorf, J., Körting, G., Jabs, W., Schweiger-Hufnagel, U., Hahner, S., ...
Analysis of complex proteomes often results in long protein lists, but falls short in measuring the validity of identification and quantification results on a greater number of proteins. Biological...
P22-S LC-MALDI Top-Down Biomarker Profiling and Identification
Brand, S., Meyer, M., Hahner, S., Suckau, D.
The search for new and validated biomarkers is of particular interest in clinical areas such as oncology, neurology, toxicology, and pharmacology. One of the challenges in finding the right...