KRAB can repress lentivirus proviral transcription independently of integration site. (2007)
Bulliard, Y, Wiznerowicz, M, Barde, I, Trono, D
The KRAB transcriptional repressor domain, commonly found in zinc finger proteins, acts by inducing the formation of heterochromatin. We previously exploited this property to achieve drug-regulated...
Wiznerowicz, M., Jakobsson, J., Szulc, J., Liao, S., Quazzola, A., Beermann, F., ...
The Krüppel-associated box (KRAB) domain is a transcriptional repression module responsible for the DNA binding-dependent gene silencing activity of hundreds of vertebrate zinc finger proteins. We...
Deglon, N., Tseng, J. L., Bensadoun, J. C., Zurn, A. D., Arsenijevic, Y., ...
Glial cell line-derived neurotrophic factor (GDNF) is able to protect dopaminergic neurons against various insults and constitutes therefore a promising candidate for the treatment of Parkinson's...
Kostic, C., Chiodini, F., Salmon, P., Wiznerowicz, M., Deglon, N., Hornfeld, D., ...
For most retinal degeneration disorders, no efficient treatment exists to preserve photoreceptors (PRs) and, consequently, to maintain vision. Gene transfer appears to be a promising approach to...
Multiply attenuated lentiviral vector achieves efficient gene delivery in vivo. (2006)
Zufferey, R, Nagy, D, Mandel, R J, Naldini, L, Trono, D
Retroviral vectors derived from lentiviruses such as HIV-1 are promising tools for human gene therapy because they mediate the in vivo delivery and long-term expression of transgenes in nondividing...
Self-inactivating lentivirus vector for safe and efficient in vivo gene delivery. (2006)
Zufferey, R, Dull, T, Mandel, R J, Bukovsky, A, Quiroz, D, Naldini, L, ...
In vivo transduction of nondividing cells by human immunodeficiency virus type 1 (HIV-1)-based vectors results in transgene expression that is stable over several months. However, the use of HIV-1...
A third-generation lentivirus vector with a conditional packaging system. (2006)
Dull, T, Zufferey, R, Kelly, M, Mandel, R J, Nguyen, M, Trono, D, ...
Vectors derived from human immunodeficiency virus (HIV) are highly efficient vehicles for in vivo gene delivery. However, their biosafety is of major concern. Here we exploit the complexity of the...
Dupraz, P, Rinsch, C, Pralong, W F, Rolland, E, Zufferey, R, Trono, D, ...
betaTC-tet cells are conditionally immortalized pancreatic beta cells which can confer long-term correction of hyperglycemia when transplanted in syngeneic streptozocin diabetic mice. The use of...
Han, J J, Mhatre, A N, Wareing, M, Pettis, R, Gao, W Q, Zufferey, R N, ...
The utility of lentivirus as a gene delivery vector in the cochlea was evaluated in vitro and in vivo. Lentivirus transduction was assessed through expression analysis of a reporter gene, green...
Zufferey, R, Donello, J E, Trono, D, Hope, T J
The expression of genes delivered by retroviral vectors is often inefficient, a potential obstacle for their widespread use in human gene therapy. Here, we explored the possibility that the...
Lentiviral gene transfer into primary and secondary NOD/SCID repopulating cells. (2006)
Woods, N B, Fahlman, C, Mikkola, H, Hamaguchi, I, Olsson, K, Zufferey, R, ...
The ability of lentiviral vectors to transfer genes into human hematopoietic stem cells was studied, using a human immunodeficiency virus 1 (HIV-1)-derived vector expressing the green fluorescence...
Saulnier, S O, Steinhoff, D, Dinauer, M C, Zufferey, R, Trono, D, Seger, R A, ...
BACKGROUND: Chronic granulomatous diseases (CGD) are caused by impaired antimicrobial activity in phagocytes, due to the absence or malfunction of the respiratory burst NADPH oxidase. Two-thirds of...
Lentivirus vector gene expression during ES cell-derived hematopoietic development in vitro. (2006)
Hamaguchi, I, Woods, N B, Panagopoulos, I, Andersson, E, Mikkola, H, Fahlman, C, ...
The murine embryonal stem (ES) cell virus (MESV) can express transgenes from the long terminal repeat (LTR) promoter/enhancer in undifferentiated ES cells, but expression is turned off upon...
A stable system for the high-titer production of multiply attenuated lentiviral vectors. (2006)
Klages, N, Zufferey, R, Trono, D
Lentiviral vectors open exciting perspectives for the genetic treatment of a wide array of inherited and acquired diseases, owing to their ability to govern the efficient delivery, integration, and...
Peng, K W, Pham, L, Ye, H, Zufferey, R, Trono, D, Cosset, F L, ...
Lentiviral vectors represent an attractive technology platform from which to develop a targetable injectable gene delivery system for transduction of specific cell populations in vivo, irrespective...
Modulation of ATM gene expression in a murine mammary epithelial cell line (2000)
Vutskits, GV, Salmon, P, Soriano, JV, Cudre-Mauroux, C, Trono, D, Montesano, R, ...
No abstract available.
Naldini, L, Blömer, U, Gage, F H, Trono, D, Verma, I M
We describe the construction of a safe, replication-defective and efficient lentiviral vector suitable for in vivo gene delivery. The reverse transcription of the vector was found to be a...
Von Schwedler, U, Kornbluth, R S, Trono, D
Lentiviruses, including human immunodeficiency virus type 1 (HIV-1), are unusual among retroviruses in their ability to infect nondividing cells. The matrix proteins of several lentiviruses contain a...
Seshamma, T, Bagasra, O, Trono, D, Baltimore, D, Pomerantz, R J
Human immunodeficiency virus type 1 (HIV-1) infections of humans have a natural history characterized by a variable but usually slow progression to an immunodeficient state. We have described a...
Koval, V, Jault, F M, Pal, P G, Moreno, T N, Aiken, C, Trono, D, ...
Human cytomegalovirus (HCMV) has been implicated as a potential cofactor in human immunodeficiency virus type 1 (HIV-1)-related disease. Previously, we reported that HCMV inhibits HIV-1 RNA and...
Nef stimulates human immunodeficiency virus type 1 proviral DNA synthesis.
The Nef protein of human immunodeficiency virus type 1 (HIV-1) stimulates viral infectivity. The mechanism of this phenotype was investigated. Viruses containing disrupted nef genes were 4 to 40...
Role of the karyopherin pathway in human immunodeficiency virus type 1 nuclear import.
Gallay, P, Stitt, V, Mundy, C, Oettinger, M, Trono, D
The interaction of the human immunodeficiency virus type 1 (HIV-1) nucleoprotein complex with the cell nuclear import machinery is necessary for viral replication in macrophages and for the...
Characterization of human immunodeficiency virus type 1 Vif particle incorporation.
The human immunodeficiency virus type 1 (HIV-1) Vif protein is necessary at the time of viral particle formation yet functionally manifests its effect after virions enter target cells. This suggests...
Gulizia, R J, Collman, R G, Levy, J A, Trono, D, Mosier, D E
The pathogenicity of four human immunodeficiency virus type 1 (HIV-1) isolates with nef deleted for SCID mice repopulated with human peripheral blood leukocytes (hu-PBL-SCID mice) was studied....
Swingler, S, Gallay, P, Camaur, D, Song, J, Abo, A, Trono, D
The human immunodeficiency virus type 1 matrix (MA) protein is phosphorylated during virion maturation on its C-terminal tyrosine and on several serine residues. Whereas MA tyrosine phosphorylation...
Highly efficient and sustained gene transfer in adult neurons with a lentivirus vector.
Blömer, U, Naldini, L, Kafri, T, Trono, D, Verma, I M, Gage, F H
The identification of monogenic and complex genes responsible for neurological disorders requires new approaches for delivering therapeutic protein genes to significant numbers of cells in the...
Camaur, D, Gallay, P, Swingler, S, Trono, D
During virus assembly, a subset of human immunodeficiency virus (HIV) matrix (MA) molecules is phosphorylated on C-terminal tyrosine. This modification facilitates infection of nondividing cells by...
Vif is crucial for human immunodeficiency virus type 1 proviral DNA synthesis in infected cells.
Von Schwedler, U, Song, J, Aiken, C, Trono, D
The human immunodeficiency virus type 1 (HIV-1) vif gene encodes a 23-kDa protein of unknown function, also produced by most other known lentiviruses. Vif was found to be essential for the spread of...
Rodent cells present two blocks precluding the expression of the human immunodeficiency virus type 1 (HIV-1) genome. First, the viral protein Tat is only poorly active in these cells. Second, when...
Pomerantz, R J, Seshamma, T, Trono, D
The Rev protein of human immunodeficiency virus type 1 (HIV-1) is essential for the expression of the structural genes of HIV-1. To determine whether a functional threshold level of Rev is required...
Partial reverse transcripts in virions from human immunodeficiency and murine leukemia viruses.
Reverse transcription of the retroviral genome is thought to start after virions enter target cells. Purified preparations of human immunodeficiency virus were found to contain virus-specific DNA,...
Andino, R, Rieckhof, G E, Trono, D, Baltimore, D
The poliovirus mutant 5NC-11 has a 4-base insertion at position 70 within the 5' untranslated region and is deficient in RNA synthesis. Revertants from 5NC-11 were isolated, showing a partial...
Trono, D, Andino, R, Baltimore, D
Twenty-one mutations were engineered in the 5' noncoding region of poliovirus type 1 RNA, using an infectious cDNA copy of the viral genome. RNA was made from these constructs and used to transfect...
Foti, M, Carpentier, J L, Aiken, C, Trono, D, Lew, D P, Krause, K H
CD4, a member of the immunoglobulin superfamily, is not only expressed in T4 helper lymphocytes but also in myeloid cells. Receptor-mediated endocytosis plays a crucial role in the regulation of...
A human cell factor is essential for HIV-1 Rev action.
To examine the restriction of HIV growth in murine cells, we infected NIH 3T3 cells with HIV pseudotyped by Moloney murine leukemia virus. The virus, which carried a dominant selectable marker under...
Mechanism of Nef-induced CD4 endocytosis: Nef connects CD4 with the mu chain of adaptor complexes.
Piguet, V, Chen, Y L, Mangasarian, A, Foti, M, Carpentier, J L, Trono, D
The Nef protein of primate lentiviruses down-regulates the cell surface expression of CD4 and probably MHC I by connecting these receptors with the endocytic machinery. Here, we reveal that Nef...
Naldini, L, Blömer, U, Gage, F H, Trono, D, Verma, I M
We describe the construction of a safe, replication-defective and efficient lentiviral vector suitable for in vivo gene delivery. The reverse transcription of the vector was found to be a...
Von Schwedler, U, Kornbluth, R S, Trono, D
Lentiviruses, including human immunodeficiency virus type 1 (HIV-1), are unusual among retroviruses in their ability to infect nondividing cells. The matrix proteins of several lentiviruses contain a...
Seshamma, T, Bagasra, O, Trono, D, Baltimore, D, Pomerantz, R J
Human immunodeficiency virus type 1 (HIV-1) infections of humans have a natural history characterized by a variable but usually slow progression to an immunodeficient state. We have described a...
Koval, V, Jault, F M, Pal, P G, Moreno, T N, Aiken, C, Trono, D, ...
Human cytomegalovirus (HCMV) has been implicated as a potential cofactor in human immunodeficiency virus type 1 (HIV-1)-related disease. Previously, we reported that HCMV inhibits HIV-1 RNA and...
Nef stimulates human immunodeficiency virus type 1 proviral DNA synthesis.
The Nef protein of human immunodeficiency virus type 1 (HIV-1) stimulates viral infectivity. The mechanism of this phenotype was investigated. Viruses containing disrupted nef genes were 4 to 40...
Role of the karyopherin pathway in human immunodeficiency virus type 1 nuclear import.
Gallay, P, Stitt, V, Mundy, C, Oettinger, M, Trono, D
The interaction of the human immunodeficiency virus type 1 (HIV-1) nucleoprotein complex with the cell nuclear import machinery is necessary for viral replication in macrophages and for the...
Characterization of human immunodeficiency virus type 1 Vif particle incorporation.
The human immunodeficiency virus type 1 (HIV-1) Vif protein is necessary at the time of viral particle formation yet functionally manifests its effect after virions enter target cells. This suggests...
Gulizia, R J, Collman, R G, Levy, J A, Trono, D, Mosier, D E
The pathogenicity of four human immunodeficiency virus type 1 (HIV-1) isolates with nef deleted for SCID mice repopulated with human peripheral blood leukocytes (hu-PBL-SCID mice) was studied....
Swingler, S, Gallay, P, Camaur, D, Song, J, Abo, A, Trono, D
The human immunodeficiency virus type 1 matrix (MA) protein is phosphorylated during virion maturation on its C-terminal tyrosine and on several serine residues. Whereas MA tyrosine phosphorylation...
Highly efficient and sustained gene transfer in adult neurons with a lentivirus vector.
Blömer, U, Naldini, L, Kafri, T, Trono, D, Verma, I M, Gage, F H
The identification of monogenic and complex genes responsible for neurological disorders requires new approaches for delivering therapeutic protein genes to significant numbers of cells in the...
Camaur, D, Gallay, P, Swingler, S, Trono, D
During virus assembly, a subset of human immunodeficiency virus (HIV) matrix (MA) molecules is phosphorylated on C-terminal tyrosine. This modification facilitates infection of nondividing cells by...
Vif is crucial for human immunodeficiency virus type 1 proviral DNA synthesis in infected cells.
Von Schwedler, U, Song, J, Aiken, C, Trono, D
The human immunodeficiency virus type 1 (HIV-1) vif gene encodes a 23-kDa protein of unknown function, also produced by most other known lentiviruses. Vif was found to be essential for the spread of...
Rodent cells present two blocks precluding the expression of the human immunodeficiency virus type 1 (HIV-1) genome. First, the viral protein Tat is only poorly active in these cells. Second, when...
Pomerantz, R J, Seshamma, T, Trono, D
The Rev protein of human immunodeficiency virus type 1 (HIV-1) is essential for the expression of the structural genes of HIV-1. To determine whether a functional threshold level of Rev is required...
Partial reverse transcripts in virions from human immunodeficiency and murine leukemia viruses.
Reverse transcription of the retroviral genome is thought to start after virions enter target cells. Purified preparations of human immunodeficiency virus were found to contain virus-specific DNA,...
Andino, R, Rieckhof, G E, Trono, D, Baltimore, D
The poliovirus mutant 5NC-11 has a 4-base insertion at position 70 within the 5' untranslated region and is deficient in RNA synthesis. Revertants from 5NC-11 were isolated, showing a partial...
Trono, D, Andino, R, Baltimore, D
Twenty-one mutations were engineered in the 5' noncoding region of poliovirus type 1 RNA, using an infectious cDNA copy of the viral genome. RNA was made from these constructs and used to transfect...
Foti, M, Carpentier, J L, Aiken, C, Trono, D, Lew, D P, Krause, K H
CD4, a member of the immunoglobulin superfamily, is not only expressed in T4 helper lymphocytes but also in myeloid cells. Receptor-mediated endocytosis plays a crucial role in the regulation of...
A human cell factor is essential for HIV-1 Rev action.
To examine the restriction of HIV growth in murine cells, we infected NIH 3T3 cells with HIV pseudotyped by Moloney murine leukemia virus. The virus, which carried a dominant selectable marker under...
Mechanism of Nef-induced CD4 endocytosis: Nef connects CD4 with the mu chain of adaptor complexes.
Piguet, V, Chen, Y L, Mangasarian, A, Foti, M, Carpentier, J L, Trono, D
The Nef protein of primate lentiviruses down-regulates the cell surface expression of CD4 and probably MHC I by connecting these receptors with the endocytic machinery. Here, we reveal that Nef...