Enzyme stability and activity at high temperatures (2008)
Daniel, R. M., Danson, M. J., Hough, D. W., Lee, C. K., Peterson, M. E., Cowan, D. A.
The 2-oxoacid dehydrogenase multienzyme complex of Haloferax volcanii (2008)
Al-Mailem, D. M., Hough, D. W., Danson, M. J.
Those aerobic archaea whose genomes have been sequenced possess four adjacent genes that, by sequence comparisons with bacteria and eukarya, appear to encode the component enzymes of a 2-oxoacid...
The structure of Sulfolobus solfataricus 2-keto-3-deoxygluconate kinase (2008)
Potter, J. A., Kerou, M., Lamble, H. J., Bull, S. D., Hough, D. W., Danson, M. J., ...
The hyperthermophilic archaeon Sulfolobus solfataricus grows optimally above 353 K and utilizes an unusual promiscuous nonphosphorylative Entner-Doudoroff pathway to metabolize both glucose and...
Heath, C., Posner, M. G., Aass, H. C., Upadhyay, A., Scott, D. J., Hough, D. W., ...
The aerobic archaea possess four closely spaced, adjacent genes that encode proteins showing significant sequence identities with the bacterial and eukaryal components comprising the 2-oxoacid...
Catalytic efficiency and k(cat)/K-M: a useful comparator? (2007)
Eisenthal, R., Danson, M. J., Hough, D. W.
The ratio k(cat)/K-M - often referred to as the 'specificity constant' - is a useful index for comparing the relative rates of an enzyme acting on alternative, competing substrates. However, an...
Milburn, C. C., Lamble, H. J., Theodossis, A., Bull, S. D., Hough, D. W., Danson, M. J., ...
The hyperthermophilic archaeon Sulfolobus solfataricus grows optimally above 80 degrees C and utilizes an unusual, promiscuous, non-phosphorylative Entner-Doudoroff pathway to metabolize both glucose...
Milburn, C. C., Lamble, H. J., Theodossis, A., Bull, S. D., Hough, D. W., Danson, M. J., ...
The hyperthermophilic archaeon Sulfolobus solfataricus grows optimally above 80 degrees C and utilizes an unusual, promiscuous, non-phosphorylative Entner-Doudoroff pathway to metabolize both glucose...
Lamble, H. J., Theodossis, A., Milburn, C. C., Taylor, G. L., Bull, S. D., Hough, D. W., ...
The hyperthermophilic archaeon Sulfolobus solfataricus metabolises glucose and galactose by a 'promiscuous' non-phosphorylative variant of the Entner-Doudoroff pathway, in which a series of enzymes...
Engineering stereocontrol into an aldolase-catalysed reaction (2005)
Lamble, H. J., Danson, M. J., Hough, D. W., Bull, S. D.
A novel thermostable aldolase has been developed for synthetic application, and substrate engineering has been used to induce stereocontrol into aldol reactions of this naturally-promiscuous enzyme.
Lamble, H. J., Theodossis, A., Milburn, C. C., Taylor, G. L., Bull, S. D., Hough, D. W., ...
The hyperthermophilic archaeon Sulfolobus solfataricus metabolises glucose and galactose by a 'promiscuous' non-phosphorylative variant of the Entner-Doudoroff pathway, in which a series of enzymes...
Engineering stereocontrol into an aldolase-catalysed reaction (2005)
Lamble, H. J., Danson, M. J., Hough, D. W., Bull, S. D.
A novel thermostable aldolase has been developed for synthetic application, and substrate engineering has been used to induce stereocontrol into aldol reactions of this naturally-promiscuous enzyme.
Theodossis, A., Walden, H., Westwick, E. J., Connaris, H., Lamble, H. J., Hough, D. W., ...
The hyperthermophilic Archaea Sulfolobus solfataricus grows optimally above 80degreesC and metabolizes glucose by a non-phosphorylative variant of the Entner-Doudoroff pathway. In this pathway...
Lamble, F. J., Milburn, C. C., Taylor, G. L., Hough, D. W., Danson, M. J.
An investigation has been carried out into gluconate dehydratase from the hyperthermophilic Archaeon Sulfolobus solfataricus. The enzyme has been purified from cell extracts of the organism and found...
Heath, C., Jeffries, A. C., Hough, D. W., Danson, M. J.
Those aerobic archaea whose genomes have been sequenced possess a single 4-gene operon that, by sequence comparisons with Bacteria and Eukarya, appears to encode the three component enzymes of a...
Multienzyme complexes in the Archaea: predictions from genome sequences (2004)
Danson, M. J., Morgan, D. J., Jefferies, A. C., Hough, D. W., Dyall-Smith, M. L.
Crenell, S.J., Higgins, C., Nawaz, S., Yeoh, L., Hough, D.W., ...
A bacterial thermostable citrate synthase has been analyzed to investigate the structural basis of its thermostability, and to compare such features with those previously identified in archaeal...
Wery, N., Gerike, U., Sharman, A., Chaudhuri, J. B., Hough, D. W., Danson, M. J.
Seventy-five aerobic heterotrophs have been isolated from a packed-column bioreactor inoculated with soil from Antarctica. The column was maintained at 10degreesC and continuously fed with a...
Lamble, H. J., Heyer, N. I., Bull, S. D., Hough, D. W., Danson, M. J.
The hyperthermophilic Archaeon Sulfolobus solfataricus metabolizes glucose by a non-phosphorylative variant of the Entner-Doudoroff pathway. In this pathway glucose dehydrogenase and gluconate...
Lamble, H. J., Heyer, N. I., Bull, S. D., Hough, D. W., Danson, M. J.
The hyperthermophilic Archaeon Sulfolobus solfataricus metabolizes glucose by a non-phosphorylative variant of the Entner-Doudoroff pathway. In this pathway glucose dehydrogenase and gluconate...
Karlsson, E. N., Abou-Hachem, M., Holst, O., Danson, M. J., Hough, D. W.
Two separate citrate synthases from the extremely thermophilic bacterium Rhodothermus marinus have been identified and purified. One of the enzymes is a hexameric protein and is the first...
Bell, G. S., Russell, R. J. M., Connaris, H., Hough, D. W., Danson, M. J., Taylor, G. L.
The crystal structure of citrate synthase from the thermophilic Archaeon Sulfolobus solfataricus (optimum growth temperature = 85 degreesC) has been determined, extending the number of crystal...
Cold-active citrate synthase: mutagenesis of active-site residues (2001)
Gerike, U., Danson, M. J., Hough, D. W.
A comparison of the crystal structure of the dimeric enzyme citrate synthase from the psychrophilic Arthrobacter strain DS2-3R with that of the structurally homologous enzyme from the...
Protein purification by ultrafiltration using a beta-galactosidase fusion tag (2000)
Sakhamuru, K., Hough, D. W., Chaudhuri, J. B.
The use of beta-galactosidase (465 kDa) as a fusion tag for ultrafiltration-based protein purification has been investigated. The target protein studied was thermophilic glucose dehydrogenase(157...
Pire, C., Camacho, M. L., Ferrer, J., Hough, D. W., Bonete, M. J.
The kinetic mechanism and metal content of Haloferax mediterranei NAD(P)(+)-glucose dehydrogenase have been investigated. The kinetic mechanism has been determined by initial rate and inhibition...
Genome sequence of Halobacterium species NRC-1 (2000)
Ng, W. V., Kennedy, S. P., Mahairas, G. G., Berquist, B., Pan, M., Shukla, H. D., ...
We report the complete sequence of an extreme halophile, Halobacterium sp. NRC-1, harboring a dynamic 2,571,010-bp genome containing 91 insertion sequences representing 12 families and organized into...
Jolley, K. A., Maddocks, D. G., Gyles, S. L., Mullan, Z., Tang, S. L., Dyall-Smith, M. L., ...
All Archaea catalyse the conversion of pyruvate to acetyl-CoA via a simple pyruvate oxidoreductase. This is in contrast to the Eukarya and most aerobic bacteria, which use the pyruvate dehydrogenase...
Hendry, E. J., Buchanan, C. L., Russell, R. J. M., Hough, D. W., Reeve, C. D., Danson, M. J., ...
Crystals have been grown of 2-keto-3-deoxygluconate aldolase (KDG aldolase) from the hyperthermophilic archaeon Sulfolobus solfataricus that diffract to 2.2 Angstrom resolution. The enzyme catalyses...
A microbiological survey of Montserrat Island hydrothermal biotopes (2000)
Atkinson, T., Gairns, S., Cowan, D. A., Danson, M. J., Hough, D. W., Johnson, D. B., ...
In March 1996, a survey of hydrothermal sites on the island of Montserrat was carried out. Six sites (Galway's Soufriere, Gages Upper and Lower Soufrieres, Hot Water Pond, Hot River, and Tar River...
Arnott, M. A., Michael, R. A., Thompson, C. R., Hough, D. W., Danson, M. J.
Citrate synthases from Thermoplasma acidophilum (optimal growth at 55 degreesC) and Pyrococcus furiosus (100 degreesC) are homo-dimeric enzymes that show a high degree of structural homology with...
Jolley, K A, Rapaport, E, Hough, D W, Danson, M J, Woods, W G, Dyall-Smith, M L
The gene encoding dihydrolipoamide dehydrogenase from the halophilic archaeon, Haloferax volcanii, has been subcloned and overexpressed in the parent organism by using the halophilic archaeal rRNA...
Smith, L D, Budgen, N, Bungard, S J, Danson, M J, Hough, D W
Glucose dehydrogenase was purified to homogeneity from the thermoacidophilic archaebacterium Thermoplasma acidophilum. The enzyme is a tetramer of polypeptide chain Mr 38,000 +/- 3000, it is...
Buchanan, C L, Connaris, H, Danson, M J, Reeve, C D, Hough, D W
Sulfolobus solfataricus is a hyperthermophilic archaeon growing optimally at 80-85 degrees C. It metabolizes glucose via a novel non-phosphorylated Entner-Doudoroff pathway, in which the reversible...
Jolley, K A, Rapaport, E, Hough, D W, Danson, M J, Woods, W G, Dyall-Smith, M L
The gene encoding dihydrolipoamide dehydrogenase from the halophilic archaeon, Haloferax volcanii, has been subcloned and overexpressed in the parent organism by using the halophilic archaeal rRNA...
Smith, L D, Budgen, N, Bungard, S J, Danson, M J, Hough, D W
Glucose dehydrogenase was purified to homogeneity from the thermoacidophilic archaebacterium Thermoplasma acidophilum. The enzyme is a tetramer of polypeptide chain Mr 38,000 +/- 3000, it is...
Buchanan, C L, Connaris, H, Danson, M J, Reeve, C D, Hough, D W
Sulfolobus solfataricus is a hyperthermophilic archaeon growing optimally at 80-85 degrees C. It metabolizes glucose via a novel non-phosphorylated Entner-Doudoroff pathway, in which the reversible...
The action of trypsin on human stored peripheral lymphocytes
Hough, D. W., Stevenson, G. T.
1. To assess the feasibility of using small lymphocytes from routinely refrigerated human blood for studies of surface biochemistry, the stored cells were compared with those from freshly drawn...
Recovery of immunoglobulin removed from lymphocytic surfaces by proteolysis
Eady, R. P., Hough, D. W., Kilshaw, P. J., Stevenson, G. T.
The removal of immunoglobulin from the surfaces of human lymphocytes by limited proteolysis has been monitored by radioimmunoassay of the supernatants and immunolabelling of the residual membrane.
Proteolysis of lymphocytic surface immunoglobulin.
Hough, D W, McIlroy, B M, Stevenson, G T
Limited proteolysis of lymphocytic surface immunoglobulins in guinea-pig, rabbit and man was investigated by immunofluorescence using conjugated antisera specific for immunoglobulin fragments. The...
Surface immunoglobulin of guinea-pig leukaemic lymphocytes.
Stevenson, G T, Eady, R P, Hough, D W, Jurd, R D, Stevenson, F K
The surface immunoglobulin of the transplantable L2C leukaemia of strain 2 guinea-pigs has been investigated. The immunoglobulin is seen to be synthesized when the cells are maintained in culture,...
Further studies of immunoglobulin synthesis by guinea pig leukaemic lymphocytes.
Hough, D W, Chapple, J C, Stevenson, F K, Stevenson, G T
The L2C leukaemia is a B-lymphocytic neoplasm of strain 2 guinea-pigs, maintained by passaging in vivo. It synthesizes mu and lambda immunoglobulin chains. These combines to form monomeric (7S) IgM...