Kwon, Dong H., Lee, Miae, Kim, J. J., Kim, J. G., Osato, M. S., ...
The prevalence of furazolidone, nitrofurantoin, and metronidazole resistance among Helicobacter pylori strains was assessed with 431 clinical isolates. Fifty-two percent were metronidazole resistant,...
Kwon, D. H., Hulten, K., Kato, M., Kim, J. J., Lee, M., ...
We previously reported that inactivation of rdxA and/or frxA converted Helicobacter pylori from metronidazole sensitive to metronidazole resistant. To examine the individual roles of rdxA and frxA in...
High-Level β-Lactam Resistance Associated with Acquired Multidrug Resistance in Helicobacter pylori
Kwon, Dong H., Dore, M. P., Kim, J. J., Kato, M., Lee, M., Wu, J. Y., ...
Four clinical Helicobacter pylori isolates with high-level resistance to β-lactams exhibited low- to moderate-level resistance to the structurally and functionally unrelated antibiotics...
Mutations in 23S rRNA are associated with clarithromycin resistance in Helicobacter pylori.
Versalovic, J, Shortridge, D, Kibler, K, Griffy, M V, Beyer, J, Flamm, R K, ...
Twelve clarithromycin-resistant Helicobacter pylori isolates (100% of resistant isolates examined) from seven different patients each contained an A-->G transition mutation within a conserved loop of...
Stone, G G, Shortridge, D, Versalovic, J, Beyer, J, Flamm, R K, Graham, D Y, ...
We have developed a rapid PCR-oligonucleotide ligation assay that can discriminate single base substitutions that are associated with clarithromycin resistance in Helicobacter pylori. Susceptible...
El-Zaatari, F A, Naser, S A, Engstrand, L, Burch, P E, Hachem, C Y, Whipple, D L, ...
Mycobacterium paratuberculosis is the causative agent of Johne's disease, a chronic enteritis in ruminants. It has also been implicated as a possible cause of Crohn's disease, an inflammatory bowel...
Proteolytic enhancement of rotavirus infectivity: molecular mechanisms.
Estes, M K, Graham, D Y, Mason, B B
The polypeptide compositions of single-shelled and double-shelled simian rotavirus particles were modified by exposure to proteolytic enzymes. Specifically, a major outer capsid polypeptide (VP3)...
Characterization of a Helicobacter pylori neutrophil-activating protein.
Evans, D J, Evans, D G, Takemura, T, Nakano, H, Lampert, H C, Graham, D Y, ...
Helicobacter pylori-associated gastritis is mainly an inflammatory cell response. In earlier work we showed that activation of human neutrophils by a cell-free water extract of H. pylori is...
Haeberle, H A, Kubin, M, Bamford, K B, Garofalo, R, Graham, D Y, El-Zaatari, F, ...
The objective of these experiments was to examine the ability of Helicobacter pylori to stimulate interleukin-10 (IL-10) or IL-12 and select for either Th1 or Th2 cells. Gastric biopsy specimens were...
Evans, D G, Karjalainen, T K, Evans, D J, Graham, D Y, Lee, C H
Gene hpaA, which codes for the receptor-binding subunit of the N-acetylneuraminyllactose-binding fibrillar hemagglutinin (NLBH) of Helicobacter pylori, was cloned and sequenced. The protein expressed...
Marchildon, P A, Ciota, L M, Zamaniyan, F Z, Peacock, J S, Graham, D Y
The diagnostic significance of the serological detection of antibodies to Helicobacter pylori has been established by numerous investigators. Reports of the clinical reliabilities of commercial...
Identification of Mycobacterium avium complex in sarcoidosis.
El-Zaatari, F A, Naser, S A, Markesich, D C, Kalter, D C, Engstand, L, Graham, D Y
Cell wall-defective bacteria which later reverted to acid-fast bacilli have been isolated from sarcoid tissue. These have not been conclusively shown to be mycobacteria. Specific PCR assays were...
Direct activity of recombinant human lactoferrin against Helicobacter pylori.
Miehlke, S, Reddy, R, Osato, M S, Ward, P P, Conneely, O M, Graham, D Y
We report the activity of recombinant human lactoferrin against Helicobacter pylori. Lactoferrin exerted a time- and dose-dependent action against 8 of the 13 clinical isolates of H. pylori tested in...
El-Zaatari, F A, Naser, S A, Graham, D Y
Johne's disease is a chronic enteritis of ruminants associated with enormous worldwide economic losses for the dairy cow- and goat-rearing industries. Management limitations and eradication programs...
Go, M F, Kapur, V, Graham, D Y, Musser, J M
Genetic diversity and relationships in 74 Helicobacter pylori isolates recovered from patients assigned to distinct clinical categories were estimated by examination of allelic variation in six genes...
Expression, self-assembly, and antigenicity of the Norwalk virus capsid protein.
Jiang, X, Wang, M, Graham, D Y, Estes, M K
Norwalk virus capsid protein was produced by expression of the second and third open reading frames of the Norwalk virus genome, using a cell-free translation system and baculovirus recombinants....
Serologic and mucosal immune response to rotavirus infection in the rabbit model.
Conner, M E, Gilger, M A, Estes, M K, Graham, D Y
We examined the humoral immune response to rotavirus infection in specific pathogen-free rabbits inoculated and challenged orally with rabbit Ala rotavirus (7.5 x 10(5) to 1 x 10(7) PFU). The humoral...
Rabbit model of rotavirus infection.
Conner, M E, Estes, M K, Graham, D Y
A new small animal model was developed to study parameters of rotavirus infections, including the active immune response. Seronegative New Zealand White rabbits (neonatal to 4 months old) were...
Biochemical mapping of the simian rotavirus SA11 genome.
Mason, B B, Graham, D Y, Estes, M K
Biochemical mapping experiments of the simian rotavirus SA11 genome were performed to determine which double-stranded RNA segment coded for each of the viral polypeptides. Viral RNA transcripts were...
Ericson, B L, Graham, D Y, Mason, B B, Estes, M K
The synthesis and processing of simian rotavirus SA11 polypeptides was investigated after infection of MA104 cells. [35S]methionine- or 3H-amino acid-labeled cell extracts were analyzed by sodium...
Urease-associated heat shock protein of Helicobacter pylori.
Evans, D J, Evans, D G, Engstrand, L, Graham, D Y
Helicobacter pylori urease is an extracellular, cell-bound enzyme with a molecular weight of approximately 600,000 (600K enzyme) comprising six 66K and six 31K subunits. A 62K protein is closely...
Evans, D G, Evans, D J, Moulds, J J, Graham, D Y
Campylobacter pylori is the causative agent of gastritis and possibly of peptic and duodenal ulcers in adults. Histological observations show C. pylori attached to gastric epithelium as well as in...
Nguyen, A M, Engstrand, L, Genta, R M, Graham, D Y, El-Zaatari, F A
To investigate whether the oral cavity is a potential reservoir and possible sanctuary for Helicobacter pylori, supragingival and subgingival plaques were analyzed by a Helicobacter genus-specific...
Gray, J J, Cunliffe, C, Ball, J, Graham, D Y, Desselberger, U, Estes, M K
Pre- and postexposure sera collected from 17 adult volunteers challenged with Norwalk virus as described previously (D. Y. Graham, X. Jiang, T. Tanaka, A. Opekun, P. Madore, and M. K. Estes, J....
Detection of antigenically distinct rotaviruses from infants.
Dimitrov, D H, Estes, M K, Rangelova, S M, Shindarov, L M, Melnick, J L, Graham, D Y
Antigenically distinct rotaviruses, i.e., viruses morphologically identical to conventional rotaviruses by electron microscopy, yet lacking the common group antigen(s) detected by an enzyme-linked...
Engstrand, L, Nguyen, A M, Graham, D Y, El-Zaatari, F A
Sequence data on Helicobacter pylori 16S rRNA were used to select two 22-base oligonucleotide primers for use in a polymerase chain reaction (PCR) for detection of H. pylori. H. pylori cells were...
Yoshimura, H H, Graham, D Y, Estes, M K, Merkal, R S
We used DNA-DNA hybridization to characterize a mycobacterial isolate, strain Linda, that was obtained from a patient with Crohn's disease and that has been reported to cause ileitis in experimental...
Detection of antibody to group B adult diarrhea rotaviruses in humans.
Nakata, S, Estes, M K, Graham, D Y, Wang, S S, Gary, G W, Melnick, J L
Group B rotaviruses have been responsible for annual epidemics of severe diarrhea affecting both adults and children in China. We developed a specific and sensitive enzyme-linked immunosorbent...
Nucleic acid hybridization studies of mycobactin-dependent mycobacteria.
Using molecular techniques, specifically, DNA-DNA hybridization in solution and measurement of the thermal stabilities of hybrids, we examined the genetic relationships among mycobactin-dependent...
Markesich, D C, Graham, D Y, Yoshimura, H H
The efficiency of culture media was compared for the culture and subculture of very slowly growing acid-fast bacilli and spheroplast forms obtained from intestinal tissues of patients with Crohn's...
Evans, D J, Evans, D G, Diaz, S R, Graham, D Y
The human erythrocyte receptor which mediates mannose-resistant hemagglutination by enterotoxigenic Escherichia coli possessing colonization factor antigen II is not universally distributed among...
Unsaturated fatty acids and viability of Helicobacter (Campylobacter) pylori.
Helicobacter (Campylobacter) pylori was found to be sensitive to the toxic effects of an unsaturated fatty acid (arachidonic acid). Data are presented that support the hypothesis that exogenous...
Detection of Norwalk virus in stool by polymerase chain reaction.
Jiang, X, Wang, J, Graham, D Y, Estes, M K
A method of reverse transcription (RT) and polymerase chain reaction (PCR) for the detection of Norwalk virus in human stools was developed. A cationic detergent, cetyltrimethylammonium bromide, was...
In vitro transcription and translation of simian rotavirus SA11 gene products.
Mason, B B, Graham, D Y, Estes, M K
Rotavirus gene products were examined, with the simian rotavirus SA11 as a model. The endogenous viral RNA-dependent RNA polymerase associated with single-shelled virus particles or with activated...
Evans, D J, Evans, D G, Smith, K E, Graham, D Y
We recently reported that Campylobacter pylori possesses a surface-associated fibrillar hemagglutinin which has an affinity for N-acetylneuraminyllactose; this hemagglutinin may function as a...
Receptor-mediated adherence of Campylobacter pylori to mouse Y-1 adrenal cell monolayers.
Evans, D G, Evans, D J, Graham, D Y
An in vitro adherence assay was developed to study the adherence of Campylobacter pylori to mammalian cells. Strains of C. pylori were isolated from individuals with gastritis. These strains...
Simian rotavirus SA11 replication in cell cultures.
Estes, M K, Graham, D Y, Gerba, C P, Smith, E M
Understanding the basic virology of rotavirus infections has been hampered by the fastidiousness of most isolates and by the lack of a rapid quantitative assay method. The growth characteristics of...
Santos, A C, Yamaoka, Y, Graham, D Y, Sepulveda, A R
Microsatellite markers permit the analysis of microsatellite instability and loss of heterozygosity. Frequently, the allelotypes of microsatellites are interpreted in the presence of numerous bands...
Comparison of methods for immunocytochemical detection of rotavirus infections.
Rotavirus infections in intestinal tissues of animals or in tissue culture cells were detected by the immunocytochemical unlabeled soluble enzyme peroxidase antiperoxidase method. Comparison of the...
Early diagnosis of signet ring cell carcinoma of the stomach: role of the Genta stain.
El-Zimaity, H M, Itani, K, Graham, D Y
Signet ring cell carcinoma is a poorly differentiated adenocarcinoma in which the tumour cells invade singly or in small groups. Early stages of the disease can be missed easily when using regular...
Graham, D Y, Genta, R M, Graham, D P, Crabtree, J E
AIM/BACKGROUND: Several studies have suggested that Helicobacter pylori which express CagA may be more virulent than those that do not, but limited populations have been studied to date. The aim of...
Antibody against Helicobacter pylori CagA and VacA and the risk for gastric cancer.
Yamaoka, Y, Kodama, T, Kashima, K, Graham, D Y
AIM: Helicobacter pylori is associated with gastric cancer. Our aim was to investigate whether CagA or VacA seropositivity provides additional risk for gastric cancer. METHODS: Sera from 110 gastric...
Modified Genta triple stain for identifying Helicobacter pylori.
El-Zimaity, H M, Wu, J, Graham, D Y
AIM: To evaluate whether lead nitrate could replace uranyl nitrate in the Genta stain for H pylori without sacrificing the advantages of the triple stain (Steiner silver impregnation combined with...
El-Zimaity, H M, Wu, J, Akamatsu, T, Graham, D Y
While an association of Helicobacter pylori infection with duodenal mucosa gastric metaplasia has been described, the details and extent of the interaction are lacking. One of the limiting factors...
Comparison of agar based media for primary isolation of Helicobacter pylori.
Hachem, C Y, Clarridge, J E, Evans, D G, Graham, D Y
AIMS--To determine the best medium for the primary isolation of Helicobacter pylori. METHODS--Sixty six gastric mucosal biopsy specimens frozen in 1 ml Cysteine Albimi media with 20% glycerol from 22...
Toothpick perforation of the intestines.
Toothpicks have been used since antiquity as instruments for mouth cleansing and as eating utensils. Toothpick injury to the gastrointestinal tract is often suspected only at the time of operation...
Kwon, Dong H., Lee, Miae, Kim, J. J., Kim, J. G., Osato, M. S., ...
The prevalence of furazolidone, nitrofurantoin, and metronidazole resistance among Helicobacter pylori strains was assessed with 431 clinical isolates. Fifty-two percent were metronidazole resistant,...
Kwon, D. H., Hulten, K., Kato, M., Kim, J. J., Lee, M., ...
We previously reported that inactivation of rdxA and/or frxA converted Helicobacter pylori from metronidazole sensitive to metronidazole resistant. To examine the individual roles of rdxA and frxA in...
High-Level β-Lactam Resistance Associated with Acquired Multidrug Resistance in Helicobacter pylori
Kwon, Dong H., Dore, M. P., Kim, J. J., Kato, M., Lee, M., Wu, J. Y., ...
Four clinical Helicobacter pylori isolates with high-level resistance to β-lactams exhibited low- to moderate-level resistance to the structurally and functionally unrelated antibiotics...
Mutations in 23S rRNA are associated with clarithromycin resistance in Helicobacter pylori.
Versalovic, J, Shortridge, D, Kibler, K, Griffy, M V, Beyer, J, Flamm, R K, ...
Twelve clarithromycin-resistant Helicobacter pylori isolates (100% of resistant isolates examined) from seven different patients each contained an A-->G transition mutation within a conserved loop of...
Stone, G G, Shortridge, D, Versalovic, J, Beyer, J, Flamm, R K, Graham, D Y, ...
We have developed a rapid PCR-oligonucleotide ligation assay that can discriminate single base substitutions that are associated with clarithromycin resistance in Helicobacter pylori. Susceptible...
El-Zaatari, F A, Naser, S A, Engstrand, L, Burch, P E, Hachem, C Y, Whipple, D L, ...
Mycobacterium paratuberculosis is the causative agent of Johne's disease, a chronic enteritis in ruminants. It has also been implicated as a possible cause of Crohn's disease, an inflammatory bowel...
Proteolytic enhancement of rotavirus infectivity: molecular mechanisms.
Estes, M K, Graham, D Y, Mason, B B
The polypeptide compositions of single-shelled and double-shelled simian rotavirus particles were modified by exposure to proteolytic enzymes. Specifically, a major outer capsid polypeptide (VP3)...
Characterization of a Helicobacter pylori neutrophil-activating protein.
Evans, D J, Evans, D G, Takemura, T, Nakano, H, Lampert, H C, Graham, D Y, ...
Helicobacter pylori-associated gastritis is mainly an inflammatory cell response. In earlier work we showed that activation of human neutrophils by a cell-free water extract of H. pylori is...
Haeberle, H A, Kubin, M, Bamford, K B, Garofalo, R, Graham, D Y, El-Zaatari, F, ...
The objective of these experiments was to examine the ability of Helicobacter pylori to stimulate interleukin-10 (IL-10) or IL-12 and select for either Th1 or Th2 cells. Gastric biopsy specimens were...
Evans, D G, Karjalainen, T K, Evans, D J, Graham, D Y, Lee, C H
Gene hpaA, which codes for the receptor-binding subunit of the N-acetylneuraminyllactose-binding fibrillar hemagglutinin (NLBH) of Helicobacter pylori, was cloned and sequenced. The protein expressed...
Marchildon, P A, Ciota, L M, Zamaniyan, F Z, Peacock, J S, Graham, D Y
The diagnostic significance of the serological detection of antibodies to Helicobacter pylori has been established by numerous investigators. Reports of the clinical reliabilities of commercial...
Identification of Mycobacterium avium complex in sarcoidosis.
El-Zaatari, F A, Naser, S A, Markesich, D C, Kalter, D C, Engstand, L, Graham, D Y
Cell wall-defective bacteria which later reverted to acid-fast bacilli have been isolated from sarcoid tissue. These have not been conclusively shown to be mycobacteria. Specific PCR assays were...
Direct activity of recombinant human lactoferrin against Helicobacter pylori.
Miehlke, S, Reddy, R, Osato, M S, Ward, P P, Conneely, O M, Graham, D Y
We report the activity of recombinant human lactoferrin against Helicobacter pylori. Lactoferrin exerted a time- and dose-dependent action against 8 of the 13 clinical isolates of H. pylori tested in...
El-Zaatari, F A, Naser, S A, Graham, D Y
Johne's disease is a chronic enteritis of ruminants associated with enormous worldwide economic losses for the dairy cow- and goat-rearing industries. Management limitations and eradication programs...
Go, M F, Kapur, V, Graham, D Y, Musser, J M
Genetic diversity and relationships in 74 Helicobacter pylori isolates recovered from patients assigned to distinct clinical categories were estimated by examination of allelic variation in six genes...
Expression, self-assembly, and antigenicity of the Norwalk virus capsid protein.
Jiang, X, Wang, M, Graham, D Y, Estes, M K
Norwalk virus capsid protein was produced by expression of the second and third open reading frames of the Norwalk virus genome, using a cell-free translation system and baculovirus recombinants....
Serologic and mucosal immune response to rotavirus infection in the rabbit model.
Conner, M E, Gilger, M A, Estes, M K, Graham, D Y
We examined the humoral immune response to rotavirus infection in specific pathogen-free rabbits inoculated and challenged orally with rabbit Ala rotavirus (7.5 x 10(5) to 1 x 10(7) PFU). The humoral...
Rabbit model of rotavirus infection.
Conner, M E, Estes, M K, Graham, D Y
A new small animal model was developed to study parameters of rotavirus infections, including the active immune response. Seronegative New Zealand White rabbits (neonatal to 4 months old) were...
Biochemical mapping of the simian rotavirus SA11 genome.
Mason, B B, Graham, D Y, Estes, M K
Biochemical mapping experiments of the simian rotavirus SA11 genome were performed to determine which double-stranded RNA segment coded for each of the viral polypeptides. Viral RNA transcripts were...
Ericson, B L, Graham, D Y, Mason, B B, Estes, M K
The synthesis and processing of simian rotavirus SA11 polypeptides was investigated after infection of MA104 cells. [35S]methionine- or 3H-amino acid-labeled cell extracts were analyzed by sodium...
Urease-associated heat shock protein of Helicobacter pylori.
Evans, D J, Evans, D G, Engstrand, L, Graham, D Y
Helicobacter pylori urease is an extracellular, cell-bound enzyme with a molecular weight of approximately 600,000 (600K enzyme) comprising six 66K and six 31K subunits. A 62K protein is closely...
Evans, D G, Evans, D J, Moulds, J J, Graham, D Y
Campylobacter pylori is the causative agent of gastritis and possibly of peptic and duodenal ulcers in adults. Histological observations show C. pylori attached to gastric epithelium as well as in...