Daisuke Okuzaki

Focused Microarray Analysis of Peripheral Mononuclear Blood Cells from Churg-Strauss Syndrome Patients (2008)

Tougan, Takahiro, Onda, Hiroaki, Okuzaki, Daisuke, Kobayashi, Shigeto, Hashimoto, Hiroshi, Nojima, Hiroshi

DNA diagnostics are useful but are hampered by difficult ethical issues. Moreover, it cannot provide enough information on the environmental factors that are important for pathogenesis of certain...

Expression Profiling of PBMC-based Diagnostic Gene Markers Isolated from Vasculitis Patients (2008)

Kobayashi, Shigeto, Ito, Akihiko, Okuzaki, Daisuke, Onda, Hiroaki, Yabuta, Norikazu, Nagamori, Ippei, ...

Vasculitis (angiitis) is a systemic autoimmune disease that often causes fatal symptoms. We aimed to isolate cDNA markers that would be useful for diagnosing not only vasculitis but also other...

Chum-RNA allows preparation of a high-quality cDNA library from a single-cell quantity of mRNA without PCR amplification (2008)

Tougan, Takahiro, Okuzaki, Daisuke, Nojima, Hiroshi

Linear RNA amplification using T7 RNA polymerase is useful in genome-wide analysis of gene expression using DNA microarrays, but exponential amplification using polymerase chain reaction (PCR) is...

Meiosis specific coiled-coil proteins in Shizosaccharomyces pombe (2007)

Ohtaka, Ayami, Saito, Takamune T, Okuzaki, Daisuke, Nojima, Hiroshi

Abstract Many meiosis-specific proteins in Schizosaccharomyces pombe contain coiled-coil motifs which play essential roles for meiotic progression. For example, the coiled-coil motifs present in...

Isolation and Expression Profiling of Genes Upregulated in Bone Marrow-Derived Mononuclear Cells of Rheumatoid Arthritis Patients (2006)

Nakamura, Nobuo, Shimaoka, Yasunori, Tougan, Takahiro, Onda, Hiroaki, Okuzaki, Daisuke, Zhao, Hanjun, ...

We have comprehensively identified the genes whose expressions are augmented in bone marrow-derived mononuclear cells (BMMC) from patients with Rheumatoid Arthritis (RA) as compared with BMMCs from...

Mcp7, a meiosis-specific coiled-coil protein of fission yeast, associates with Meu13 and is required for meiotic recombination (2004)

Saito, Takamune T., Tougan, Takahiro, Kasama, Takashi, Okuzaki, Daisuke, Nojima, Hiroshi

We previously showed that Meu13 of Schizosaccharomyces pombe functions in homologous pairing and recombination at meiosis I. Here we show that a meiosis-specific gene encodes a coiled-coil protein...

Replication Factor C3 of Schizosaccharomyces pombe, a Small Subunit of Replication Factor C Complex, Plays a Role in Both Replication and Damage Checkpoints

Shimada, Midori, Okuzaki, Daisuke, Tanaka, Seiji, Tougan, Takahiro, Tamai, Katsuyuki K., Shimoda, Chikashi, ...

We report here the isolation and functional analysis of the rfc3+ gene of Schizosaccharomyces pombe, which encodes the third subunit of replication factor C (RFC3). Because the rfc3+ gene was...

Mcp7, a meiosis-specific coiled-coil protein of fission yeast, associates with Meu13 and is required for meiotic recombination

Saito, Takamune T., Tougan, Takahiro, Kasama, Takashi, Okuzaki, Daisuke, Nojima, Hiroshi

We previously showed that Meu13 of Schizosaccharomyces pombe functions in homologous pairing and recombination at meiosis I. Here we show that a meiosis-specific gene encodes a coiled-coil protein...

Spo5/Mug12, a Putative Meiosis-Specific RNA-Binding Protein, Is Essential for Meiotic Progression and Forms Mei2 Dot-Like Nuclear Foci†

Kasama, Takashi, Shigehisa, Akira, Hirata, Aiko, Saito, Takamune T., Tougan, Takahiro, Okuzaki, Daisuke, ...

We report here a functional analysis of spo5+(mug12+) of Schizosaccharomyces pombe, which encodes a putative RNA-binding protein. The disruption of spo5+ caused abnormal sporulation, generating...

Replication Factor C3 of Schizosaccharomyces pombe, a Small Subunit of Replication Factor C Complex, Plays a Role in Both Replication and Damage Checkpoints

Shimada, Midori, Okuzaki, Daisuke, Tanaka, Seiji, Tougan, Takahiro, Tamai, Katsuyuki K., Shimoda, Chikashi, ...

We report here the isolation and functional analysis of the rfc3+ gene of Schizosaccharomyces pombe, which encodes the third subunit of replication factor C (RFC3). Because the rfc3+ gene was...

Mcp7, a meiosis-specific coiled-coil protein of fission yeast, associates with Meu13 and is required for meiotic recombination

Saito, Takamune T., Tougan, Takahiro, Kasama, Takashi, Okuzaki, Daisuke, Nojima, Hiroshi

We previously showed that Meu13 of Schizosaccharomyces pombe functions in homologous pairing and recombination at meiosis I. Here we show that a meiosis-specific gene encodes a coiled-coil protein...

Spo5/Mug12, a Putative Meiosis-Specific RNA-Binding Protein, Is Essential for Meiotic Progression and Forms Mei2 Dot-Like Nuclear Foci†

Kasama, Takashi, Shigehisa, Akira, Hirata, Aiko, Saito, Takamune T., Tougan, Takahiro, Okuzaki, Daisuke, ...

We report here a functional analysis of spo5+(mug12+) of Schizosaccharomyces pombe, which encodes a putative RNA-binding protein. The disruption of spo5+ caused abnormal sporulation, generating...

Meiosis specific coiled-coil proteins in Shizosaccharomyces pombe

Ohtaka, Ayami, Saito, Takamune T, Okuzaki, Daisuke, Nojima, Hiroshi

Many meiosis-specific proteins in Schizosaccharomyces pombe contain coiled-coil motifs which play essential roles for meiotic progression. For example, the coiled-coil motifs present in Meu13 and...

Mcp4, a Meiotic Coiled-Coil Protein, Plays a Role in F-Actin Positioning during Schizosaccharomyces pombe Meiosis▿

Ohtaka, Ayami, Okuzaki, Daisuke, Saito, Takamune T., Nojima, Hiroshi

Some meiosis-specific proteins of Schizosaccharomyces pombe harbor coiled-coil motifs and play essential roles in meiotic progression. Here we describe Mcp4, a novel meiosis-specific protein whose...

Mcp5, a meiotic cell cortex protein, is required for nuclear movement mediated by dynein and microtubules in fission yeast

Saito, Takamune T., Okuzaki, Daisuke, Nojima, Hiroshi

During meiotic prophase I of the fission yeast Schizosaccharomyces pombe, oscillatory nuclear movement occurs. This promotes homologous chromosome pairing and recombination and involves cortical...

Chum-RNA allows preparation of a high-quality cDNA library from a single-cell quantity of mRNA without PCR amplification

Tougan, Takahiro, Okuzaki, Daisuke, Nojima, Hiroshi

Linear RNA amplification using T7 RNA polymerase is useful in genome-wide analysis of gene expression using DNA microarrays, but exponential amplification using polymerase chain reaction (PCR) is...

Expression Profiling of PBMC-based Diagnostic Gene Markers Isolated from Vasculitis Patients

Kobayashi, Shigeto, Ito, Akihiko, Okuzaki, Daisuke, Onda, Hiroaki, Yabuta, Norikazu, Nagamori, Ippei, ...

Vasculitis (angiitis) is a systemic autoimmune disease that often causes fatal symptoms. We aimed to isolate cDNA markers that would be useful for diagnosing not only vasculitis but also other...

Focused Microarray Analysis of Peripheral Mononuclear Blood Cells from Churg–Strauss Syndrome Patients

Tougan, Takahiro, Onda, Hiroaki, Okuzaki, Daisuke, Kobayashi, Shigeto, Hashimoto, Hiroshi, Nojima, Hiroshi

DNA diagnostics are useful but are hampered by difficult ethical issues. Moreover, it cannot provide enough information on the environmental factors that are important for pathogenesis of certain...