Dale A. Ramsden

End-bridging is required for pol {micro} to efficiently promote repair of noncomplementary ends by nonhomologous end joining (2008)

Davis, Bryan J., Havener, Jody M., Ramsden, Dale A.

DNA polymerase μ is a member of the mammalian pol X family and reduces deletion during chromosome break repair by nonhomologous end joining (NHEJ). This biological role is linked to pol μ's ability...

WRN Exonuclease activity is blocked by specific oxidatively induced base lesions positioned in either DNA strand (2008)

Bukowy, Zuzanna, Harrigan, Jeanine A., Ramsden, Dale A., Tudek, Barbara, Bohr, Vilhelm A., Stevnsner, Tinna

Werner syndrome (WS) is a premature aging disorder caused by mutations in the WS gene (WRN). Although WRN has been suggested to play an important role in DNA metabolic pathways, such as...

Phosphorylation in the serine/threonine 2609-2647 cluster promotes but is not essential for DNA-dependent protein kinase-mediated nonhomologous end joining in human whole-cell extracts (2007)

Povirk, Lawrence F., Zhou, Rui-Zhe, Ramsden, Dale A., Lees-Miller, Susan P., Valerie, Kristoffer

Previous work suggested that phosphorylation of DNA-PKcs at several serine/threonine (S/T) residues at positions 2609–2647 promotes DNA-PK-dependent end joining. In an attempt to clarify the role...

Genomic instability due to V(D)J recombination-associated transposition (2006)

Reddy, Yeturu V.R., Perkins, Eric J., Ramsden, Dale A.

The first step in assembling immunoglobulin and T-cell receptors by V(D)J recombination has similarities to transposon excision. The excised transposon-like element then integrates into DNA targets...

Histone 3 lysine 4 methylation during the pre-B to immature B-cell transition (2004)

Perkins, Eric J., Kee, Barbara L., Ramsden, Dale A.

The relationship between chromatin modification and lymphocyte development is still poorly understood. Here we show a correlation between methylation of lysine 4 on histone 3 (H3‐K4) and...

Ku heterodimer binds to both ends of the Werner protein and functional interaction occurs at the Werner N-terminus (2002)

Karmakar, Parimal, Snowden, Carey M., Ramsden, Dale A., Bohr, Vilhelm A.

The human Werner syndrome protein, WRN, is a member of the RecQ helicase family and contains 3′→5′ helicase and 3′→5′ exonuclease activities. Recently, we showed that the exonuclease...

Conservation of sequence in recombination signal sequence spacers (1994)

Ramsden, Dale A., Baetz, Kristin, Wu, Gillian E.

The variable domains of immunoglobulins and T cell receptors are assembled through the somatic, site specific recombination of multiple germline segments (V, D, and J segments) or V(D)J...

Ku Recruits the XRCC4-Ligase IV Complex to DNA Ends

Nick McElhinny, Stephanie A., Snowden, Carey M., McCarville, Joseph, Ramsden, Dale A.

Genetic experiments have determined that Ku, XRCC4, and ligase IV are required for repair of double-strand breaks by the end-joining pathway. The last two factors form a tight complex in cells....

Ku heterodimer binds to both ends of the Werner protein and functional interaction occurs at the Werner N-terminus

Karmakar, Parimal, Snowden, Carey M., Ramsden, Dale A., Bohr, Vilhelm A.

The human Werner syndrome protein, WRN, is a member of the RecQ helicase family and contains 3′→5′ helicase and 3′→5′ exonuclease activities. Recently, we showed that the exonuclease...

Association of DNA Polymerase μ (pol μ) with Ku and Ligase IV: Role for pol μ in End-Joining Double-Strand Break Repair

Mahajan, Kiran N., Nick McElhinny, Stephanie A., Mitchell, Beverly S., Ramsden, Dale A.

Mammalian DNA polymerase μ (pol μ) is related to terminal deoxynucleotidyl transferase, but its biological role is not yet clear. We show here that after exposure of cells to ionizing radiation...

Polymerase Mu Is a DNA-Directed DNA/RNA Polymerase

Nick McElhinny, Stephanie A., Ramsden, Dale A.

DNA polymerases are defined as such because they use deoxynucleotides instead of ribonucleotides with high specificity. We show here that polymerase mu (pol μ), implicated in the nonhomologous...

Autophosphorylation of the Catalytic Subunit of the DNA-Dependent Protein Kinase Is Required for Efficient End Processing during DNA Double-Strand Break Repair

Ding, Qi, Reddy, Yeturu V. R., Wang, Wei, Woods, Timothy, Douglas, Pauline, Ramsden, Dale A., ...

The DNA-dependent protein kinase (DNA-PK) plays an essential role in nonhomologous DNA end joining (NHEJ) by initially recognizing and binding to DNA breaks. We have shown that in vitro, purified...

Histone 3 lysine 4 methylation during the pre-B to immature B-cell transition

Perkins, Eric J., Kee, Barbara L., Ramsden, Dale A.

The relationship between chromatin modification and lymphocyte development is still poorly understood. Here we show a correlation between methylation of lysine 4 on histone 3 (H3-K4) and activation...

Sensing of intermediates in V(D)J recombination by ATM

Perkins, Eric J., Nair, Ayyappan, Cowley, Dale O., Van Dyke, Terry, Chang, Yung, Ramsden, Dale A.

Ataxia-telangiectasia mutated (ATM) is required for resistance to radiation-induced DNA breaks. Here we use chromatin immunoprecipitation to show that ATM also localizes to breaks associated with...

Ku Recruits the XRCC4-Ligase IV Complex to DNA Ends

Nick McElhinny, Stephanie A., Snowden, Carey M., McCarville, Joseph, Ramsden, Dale A.

Genetic experiments have determined that Ku, XRCC4, and ligase IV are required for repair of double-strand breaks by the end-joining pathway. The last two factors form a tight complex in cells....

Ku heterodimer binds to both ends of the Werner protein and functional interaction occurs at the Werner N-terminus

Karmakar, Parimal, Snowden, Carey M., Ramsden, Dale A., Bohr, Vilhelm A.

The human Werner syndrome protein, WRN, is a member of the RecQ helicase family and contains 3′→5′ helicase and 3′→5′ exonuclease activities. Recently, we showed that the exonuclease...

Association of DNA Polymerase μ (pol μ) with Ku and Ligase IV: Role for pol μ in End-Joining Double-Strand Break Repair

Mahajan, Kiran N., Nick McElhinny, Stephanie A., Mitchell, Beverly S., Ramsden, Dale A.

Mammalian DNA polymerase μ (pol μ) is related to terminal deoxynucleotidyl transferase, but its biological role is not yet clear. We show here that after exposure of cells to ionizing radiation...

Polymerase Mu Is a DNA-Directed DNA/RNA Polymerase

Nick McElhinny, Stephanie A., Ramsden, Dale A.

DNA polymerases are defined as such because they use deoxynucleotides instead of ribonucleotides with high specificity. We show here that polymerase mu (pol μ), implicated in the nonhomologous...

Sensing of intermediates in V(D)J recombination by ATM

Perkins, Eric J., Nair, Ayyappan, Cowley, Dale O., Van Dyke, Terry, Chang, Yung, Ramsden, Dale A.

Ataxia-telangiectasia mutated (ATM) is required for resistance to radiation-induced DNA breaks. Here we use chromatin immunoprecipitation to show that ATM also localizes to breaks associated with...

Autophosphorylation of the Catalytic Subunit of the DNA-Dependent Protein Kinase Is Required for Efficient End Processing during DNA Double-Strand Break Repair

Ding, Qi, Reddy, Yeturu V. R., Wang, Wei, Woods, Timothy, Douglas, Pauline, Ramsden, Dale A., ...

The DNA-dependent protein kinase (DNA-PK) plays an essential role in nonhomologous DNA end joining (NHEJ) by initially recognizing and binding to DNA breaks. We have shown that in vitro, purified...

Histone 3 lysine 4 methylation during the pre-B to immature B-cell transition

Perkins, Eric J., Kee, Barbara L., Ramsden, Dale A.

The relationship between chromatin modification and lymphocyte development is still poorly understood. Here we show a correlation between methylation of lysine 4 on histone 3 (H3-K4) and activation...

Phosphorylation in the serine/threonine 2609–2647 cluster promotes but is not essential for DNA-dependent protein kinase-mediated nonhomologous end joining in human whole-cell extracts

Povirk, Lawrence F., Zhou, Rui-Zhe, Ramsden, Dale A., Lees-Miller, Susan P., Valerie, Kristoffer

Previous work suggested that phosphorylation of DNA-PKcs at several serine/threonine (S/T) residues at positions 2609–2647 promotes DNA-PK-dependent end joining. In an attempt to clarify the role...

End-bridging is required for pol μ to efficiently promote repair of noncomplementary ends by nonhomologous end joining

Davis, Bryan J., Havener, Jody M., Ramsden, Dale A.

DNA polymerase μ is a member of the mammalian pol X family and reduces deletion during chromosome break repair by nonhomologous end joining (NHEJ). This biological role is linked to pol μ's ability...

WRN Exonuclease activity is blocked by specific oxidatively induced base lesions positioned in either DNA strand

Bukowy, Zuzanna, Harrigan, Jeanine A., Ramsden, Dale A., Tudek, Barbara, Bohr, Vilhelm A., Stevnsner, Tinna

Werner syndrome (WS) is a premature aging disorder caused by mutations in the WS gene (WRN). Although WRN has been suggested to play an important role in DNA metabolic pathways, such as...