Daniel Charlier

Ss-LrpB, a transcriptional regulator from Sulfolobus solfataricus, regulates a gene cluster with a pyruvate ferredoxin oxidoreductase-encoding operon and permease genes (2009)

Charlier, Daniel, Driessen, Arnold J.M., Vassart, Amelia, Albers, Sonja-Verena, Peeters, Eveline

Ss-LrpB is an Lrp-like transcriptional regulator from Sulfolobus solfataricus. Previously, in vitro binding of Ss-LrpB to the control region of its own gene has been extensively studied. However,...

Insights into the architecture and stoichiometry of Escherichia coli PepA*DNA complexes involved in transcriptional control and site-specific DNA recombination by atomic force microscopy (2009)

Minh, Phu Nguyen Le, Devroede, Neel, Massant, Jan, Maes, Dominique, Charlier, Daniel

Multifunctional Aminopeptidase A (PepA) from Escherichia coli is involved in the control of two distinct DNA transaction processes: transcriptional repression of the carAB operon, encoding carbamoyl...

Analysis of the DNA-binding sequence specificity of the archaeal transcriptional regulator Ss-LrpB from Sulfolobus solfataricus by systematic mutagenesis and high resolution contact probing (2007)

Peeters, Eveline, Wartel, Carine, Maes, Dominique, Charlier, Daniel

To determine the sequence specificity of dimeric Ss-LrpB, a high resolution contact map was constructed and a saturation mutagenesis conducted on one half of the palindromic consensus box....

Analysis of the DNA-binding sequence specificity of the archaeal transcriptional regulator Ss-LrpB from Sulfolobus solfataricus by systematic mutagenesis and high resolution contact probing (2006)

Peeters, Eveline, Wartel, Carine, Maes, Dominique, Charlier, Daniel

To determine the sequence specificity of dimeric Ss-LrpB, a high resolution contact map was constructed and a saturation mutagenesis conducted on one half of the palindromic consensus box....

Analysis of the DNA-binding sequence specificity of the archaeal transcriptional regulator Ss-LrpB from Sulfolobus solfataricus by systematic mutagenesis and high resolution contact probing (2006)

Peeters, Eveline, Wartel, Carine, Maes, Dominique, Charlier, Daniel

To determine the sequence specificity of dimeric Ss-LrpB, a high resolution contact map was constructed and a saturation mutagenesis conducted on one half of the palindromic consensus box....

IS3 can function as a mobile promoter in E. coli (1982)

Charlier, Daniel, Piette, Jacques, Glansdorff, Nicolas

We had shown previously (3) that the E. coli argE gene could be turned-on by an IS3 element inserted in orientation II near the 5′ end of the gene. Here we show that this effect is due to the...

The regulatory region of the divergent argECBH operon in Escherichia coliK-12 (1982)

Piette, Jacques, Cunin, Raymond, Boyen, Anne, Charlier, Daniel, Crabeel, Marjolaine, Vliet, Françoise Van, ...

The nucleotide sequence of the control region of the divergent argECBH operon has been established in the wild type and in mutants affecting expression of these genes. The argE and argCBH promoters...

Deletions and DNA rearrangements within the transposable DNA element IS2. A model for the creation of palindromic DNA by DNA repair synthesis (1980)

Besemer, Jürgen, Görtz, Gabriele, Charlier, Daniel

Three derivatives of mutant galOP-308::IS2-I of Escherichia coli were characterized by DNA sequence analysis. Deletions and DNA sequence rearrangements were observed which apparently were initiated...

Purification and Characterization of Sa-Lrp, a DNA-Binding Protein from the Extreme Thermoacidophilic Archaeon Sulfolobus acidocaldarius Homologous to the Bacterial Global Transcriptional Regulator Lrp

Enoru-Eta, Julius, Gigot, Daniel, Thia-Toong, Thia-Lin, Glansdorff, Nicolas, Charlier, Daniel

Archaea, constituting the third primary domain of life, harbor a basal transcription apparatus of the eukaryotic type, whereas curiously, a large fraction of the potential transcription regulation...

Construction of a Shuttle Vector for, and Spheroplast Transformation of, the Hyperthermophilic Archaeon Pyrococcus abyssi

Lucas, Soizick, Toffin, Laurent, Zivanovic, Yvan, Charlier, Daniel, Moussard, Hélène, Forterre, Patrick, ...

Our understanding of the genetics of species of the best-studied hyperthermophilic archaea, Pyrococcus spp., is presently limited by the lack of suitable genetic tools, such as a stable cloning...

Genes of De Novo Pyrimidine Biosynthesis from the Hyperthermoacidophilic Crenarchaeote Sulfolobus acidocaldarius: Novel Organization in a Bipolar Operon

Thia-Toong, Thia-Lin, Roovers, Martine, Durbecq, Virginie, Gigot, Daniel, Glansdorff, Nicolas, Charlier, Daniel

Sequencing a 8,519-bp segment of the Sulfolobus acidocaldarius genome revealed the existence of a tightly packed bipolar pyrimidine gene cluster encoding the enzymes of de novo UMP synthesis. The G+C...

Mutational Analysis of Intervening Sequences Connecting the Binding Sites for Integration Host Factor, PepA, PurR, and RNA Polymerase in the Control Region of the Escherichia coli carAB Operon, Encoding Carbamoylphosphate Synthase†

Devroede, Neel, Huysveld, Nadine, Charlier, Daniel

Transcription of the carAB operon encoding the unique carbamoylphosphate synthase of Escherichia coli reflects the dual function of carbamoylphosphate in the biosynthesis of arginine and pyrimidine...

Turn-on of Inactive Genes by Promoter Recruitment in ESCHERICHIA COLI: Inverted Repeats Resulting in Artificial Divergent Operons

Charlier, Daniel, Severne, Yvonne, Zafarullah, Muhamad, Glansdorff, Nicolas

We have characterized two rearrangements consisting of inverted repeats of the argE gene. The promoters (p) of argE and of argCBH face each other over an internal operator. The rearrangements were...

Purification and Characterization of Sa-Lrp, a DNA-Binding Protein from the Extreme Thermoacidophilic Archaeon Sulfolobus acidocaldarius Homologous to the Bacterial Global Transcriptional Regulator Lrp

Enoru-Eta, Julius, Gigot, Daniel, Thia-Toong, Thia-Lin, Glansdorff, Nicolas, Charlier, Daniel

Archaea, constituting the third primary domain of life, harbor a basal transcription apparatus of the eukaryotic type, whereas curiously, a large fraction of the potential transcription regulation...

Construction of a Shuttle Vector for, and Spheroplast Transformation of, the Hyperthermophilic Archaeon Pyrococcus abyssi

Lucas, Soizick, Toffin, Laurent, Zivanovic, Yvan, Charlier, Daniel, Moussard, Hélène, Forterre, Patrick, ...

Our understanding of the genetics of species of the best-studied hyperthermophilic archaea, Pyrococcus spp., is presently limited by the lack of suitable genetic tools, such as a stable cloning...

Genes of De Novo Pyrimidine Biosynthesis from the Hyperthermoacidophilic Crenarchaeote Sulfolobus acidocaldarius: Novel Organization in a Bipolar Operon

Thia-Toong, Thia-Lin, Roovers, Martine, Durbecq, Virginie, Gigot, Daniel, Glansdorff, Nicolas, Charlier, Daniel

Sequencing a 8,519-bp segment of the Sulfolobus acidocaldarius genome revealed the existence of a tightly packed bipolar pyrimidine gene cluster encoding the enzymes of de novo UMP synthesis. The G+C...

Turn-on of Inactive Genes by Promoter Recruitment in ESCHERICHIA COLI: Inverted Repeats Resulting in Artificial Divergent Operons

Charlier, Daniel, Severne, Yvonne, Zafarullah, Muhamad, Glansdorff, Nicolas

We have characterized two rearrangements consisting of inverted repeats of the argE gene. The promoters (p) of argE and of argCBH face each other over an internal operator. The rearrangements were...

Mutational Analysis of Intervening Sequences Connecting the Binding Sites for Integration Host Factor, PepA, PurR, and RNA Polymerase in the Control Region of the Escherichia coli carAB Operon, Encoding Carbamoylphosphate Synthase†

Devroede, Neel, Huysveld, Nadine, Charlier, Daniel

Transcription of the carAB operon encoding the unique carbamoylphosphate synthase of Escherichia coli reflects the dual function of carbamoylphosphate in the biosynthesis of arginine and pyrimidine...

Analysis of the DNA-binding sequence specificity of the archaeal transcriptional regulator Ss-LrpB from Sulfolobus solfataricus by systematic mutagenesis and high resolution contact probing

Peeters, Eveline, Wartel, Carine, Maes, Dominique, Charlier, Daniel

To determine the sequence specificity of dimeric Ss-LrpB, a high resolution contact map was constructed and a saturation mutagenesis conducted on one half of the palindromic consensus box....

Overexpression, purification, crystallization and preliminary X-ray diffraction analysis of the C-­terminal domain of Ss-LrpB, a transcription regulator from Sulfolobus solfataricus

Peeters, Eveline, Hoa, Bach Thi Mai, Zegers, Ingrid, Charlier, Daniel, Maes, Dominique

The C-terminal domain of the transcriptional regulator Ss-LrpB from S. solfataricus was purified by affinity chromatography and crystallized. Crystals belong to space group P21212. A complete data...

Crystallization and preliminary X-ray diffraction analysis of the arginine repressor of the hyperthermophile Thermotoga neapolitana

Massant, Jan, Peeters, Eveline, Charlier, Daniel, Maes, Dominique

The arginine repressor of the hyperthermophile T. neapolitana was crystallized with and without its corepressor arginine. Both crystals diffracted to high resolution and belong to the orthorhombic...

Insights into the architecture and stoichiometry of Escherichia coli PepA•DNA complexes involved in transcriptional control and site-specific DNA recombination by atomic force microscopy

Minh, Phu Nguyen Le, Devroede, Neel, Massant, Jan, Maes, Dominique, Charlier, Daniel

Multifunctional Aminopeptidase A (PepA) from Escherichia coli is involved in the control of two distinct DNA transaction processes: transcriptional repression of the carAB operon, encoding carbamoyl...