Daniel J. Müller, Max Kessler, Clemens Möller, Dieter Oesterhelt, Hermann Gaub
ABSTRACT The combination of high-resolution atomic force microscopy imaging and single-molecule force spectroscopy allows the identification, selection, and mechanical investigation of individual...
Single-cell force spectroscopy (2008)
Helenius, Jonne, Heisenberg, Carl-Philipp, Gaub, Hermann E, Müller, Daniel J
The controlled adhesion of cells to each other and to the extracellular matrix is crucial for tissue development and maintenance. Numerous assays have been developed to quantify cell adhesion. Among...
Harald Janovjak, Daniel J. Müller
ABSTRACT Recent advances in atomic force microscopy allowed globular and membrane proteins to be mechanically unfolded on a single-molecule level. Presented is an extension to the existing force...
Clemens M. Franz, Daniel J. Müller
Atomic force microscopy (AFM) can produce highresolution topographic images of biological samples in physiologically relevant environments and is therefore well suited for the imaging of cellular...
Probing the energy landscape of the membrane protein bacteriorhodopsin. Structure (2004)
Harald Janovjak, Jens Struckmeier, Maurice Hubain, Alexej Kedrov, Max Kessler, Daniel J. Müller
through the specific folding of their polypeptide in the anisotropic environment of the lipid bilayer (Haltia and
Philippsen, Ansgar, Im, Wonpil, Engel, Andreas, Schirmer, Tilman, Roux, Benoit, Müller, Daniel J.
The atomic force microscope (AFM) was used to image native OmpF porin and to detect the electrostatic potential generated by the protein. To this end the OmpF porin trimers from Escherichia coli was...
Conformational changes in surface structures of isolated connexin 26 gap junctions (2002)
Müller, Daniel J., Hand, Galen M., Engel, Andreas, Sosinsky, Gina E.
Gap junction channels mediate communication between adjacent cells. Using atomic force microscopy (AFM), we have imaged conformational changes of the cytoplasmic and extracellular surfaces of native...
Biomolecular imaging using atomic force microscopy (2002)
Müller, Daniel J., Anderson, Kurt
Atomic force microscopy (AFM) has become a well-established technique for imaging single biomacromolecules under physiological conditions. The exceptionally high spatial resolution and...
Imaging and manipulation of biological structures with the AFM (2002)
Fotiadis, Dimitrios, Scheuring, Simon, Müller, Shirley A., Engel, Andreas, Müller, Daniel J.
Many biologists have dreamt of physically touching and manipulating the biomolecules they were investigating. With the invention of the atomic force microscope (AFM), this dream has come true. Here,...
Identification and structure of a putative Ca2+-binding domain at the C terminus of AQP1 (2002)
Fotiadis, Dimitrios, Suda, Kitaru, Tittmann, Peter, Jeno, Paul, Philippsen, Ansgar, Müller, Daniel J., ...
um in translocation of AQP1 in cholangiocytes from intracellular vesicles to the plasma membrane and in triggering its fusion is discussed. Functional studies are now required to identify the...
Observing structure, function and assembly of single proteins by AFM (2002)
Müller, Daniel J., Janovjak, Harald, Lehto, Tina, Kuerschner, Lars, Anderson, Kurt
Single molecule experiments provide insight into the individuality of biological macromolecules, their unique function, reaction pathways, trajectories and molecular interactions. The exceptional...
Observing structure, function and assembly of single proteins by AFM (2002)
Müller, Daniel J., Janovjak, Harald, Lehto, Tina, Kuerschner, Lars, Anderson, Kurt
Single molecule experiments provide insight into the individuality of biological macromolecules, their unique function, reaction pathways, trajectories and molecular interactions. The exceptional...
Cichon, Sven, Schumacher, Johannes, Müller, Daniel J., Hürter, Martina, Windemuth, Christine, Strauch, Konstantin, ...
Cichon, Sven, Schumacher, Johannes, Müller, Daniel J., Hürter, Martina, Windemuth, Christine, Strauch, Konstantin, ...
Bipolar affective disorder (BPAD), also known as manic depressive illness, is a severe psychiatric disorder characterized by episodes of mania and depression. It has a lifetime prevalence of...
Controlled unzipping of a bacterial surface layer with atomic force microscopy
Müller, Daniel J., Baumeister, Wolfgang, Engel, Andreas
We have combined high-resolution atomic force microscopy (AFM) imaging and force spectroscopy to gain insight into the interaction forces between the individual protomers of the hexagonally packed...
Conformational changes in surface structures of isolated connexin 26 gap junctions
Müller, Daniel J., Hand, Galen M., Engel, Andreas, Sosinsky, Gina E.
Gap junction channels mediate communication between adjacent cells. Using atomic force microscopy (AFM), we have imaged conformational changes of the cytoplasmic and extracellular surfaces of native...
Unfolding pathways of native bacteriorhodopsin depend on temperature
Janovjak, Harald, Kessler, Max, Oesterhelt, Dieter, Gaub, Hermann, Müller, Daniel J.
The combination of high-resolution atomic force microscopy (AFM) imaging and single-molecule force-spectroscopy was employed to unfold single bacteriorhodopsins (BR) from native purple membrane...
Bacterial Na+-ATP synthase has an undecameric rotor
Stahlberg, Henning, Müller, Daniel J., Suda, Kitaru, Fotiadis, Dimitrios, Engel, Andreas, Meier, Thomas, ...
Synthesis of adenosine triphosphate (ATP) by the F1F0 ATP synthase involves a membrane-embedded rotary engine, the F0 domain, which drives the extra-membranous catalytic F1 domain. The F0 domain...
Philippsen, Ansgar, Im, Wonpil, Engel, Andreas, Schirmer, Tilman, Roux, Benoit, Müller, Daniel J
The atomic force microscope (AFM) was used to image native OmpF porin and to detect the electrostatic potential generated by the protein. To this end the OmpF porin trimers from Escherichia coli was...
Müller, Daniel J, Kessler, Max, Oesterhelt, Filipp, Möller, Clemens, Oesterhelt, Dieter, Gaub, Hermann
The combination of high-resolution atomic force microscopy imaging and single-molecule force spectroscopy allows the identification, selection, and mechanical investigation of individual proteins. In...
Molecular Force Modulation Spectroscopy Revealing the Dynamic Response of Single Bacteriorhodopsins
Janovjak, Harald, Müller, Daniel J., Humphris, Andrew D. L.
Recent advances in atomic force microscopy allowed globular and membrane proteins to be mechanically unfolded on a single-molecule level. Presented is an extension to the existing force spectroscopy...
Complex Stability of Single Proteins Explored by Forced Unfolding Experiments
Janovjak, Harald, Sapra, K. Tanuj, Müller, Daniel J.
In the last decade atomic force microscopy has been used to measure the mechanical stability of single proteins. These force spectroscopy experiments have shown that many water-soluble and membrane...
Controlled unzipping of a bacterial surface layer with atomic force microscopy
Müller, Daniel J., Baumeister, Wolfgang, Engel, Andreas
We have combined high-resolution atomic force microscopy (AFM) imaging and force spectroscopy to gain insight into the interaction forces between the individual protomers of the hexagonally packed...
Conformational changes in surface structures of isolated connexin 26 gap junctions
Müller, Daniel J., Hand, Galen M., Engel, Andreas, Sosinsky, Gina E.
Gap junction channels mediate communication between adjacent cells. Using atomic force microscopy (AFM), we have imaged conformational changes of the cytoplasmic and extracellular surfaces of native...
Unfolding pathways of native bacteriorhodopsin depend on temperature
Janovjak, Harald, Kessler, Max, Oesterhelt, Dieter, Gaub, Hermann, Müller, Daniel J.
The combination of high-resolution atomic force microscopy (AFM) imaging and single-molecule force-spectroscopy was employed to unfold single bacteriorhodopsins (BR) from native purple membrane...
Bacterial Na+-ATP synthase has an undecameric rotor
Stahlberg, Henning, Müller, Daniel J., Suda, Kitaru, Fotiadis, Dimitrios, Engel, Andreas, Meier, Thomas, ...
Synthesis of adenosine triphosphate (ATP) by the F1F0 ATP synthase involves a membrane-embedded rotary engine, the F0 domain, which drives the extra-membranous catalytic F1 domain. The F0 domain...
Philippsen, Ansgar, Im, Wonpil, Engel, Andreas, Schirmer, Tilman, Roux, Benoit, Müller, Daniel J
The atomic force microscope (AFM) was used to image native OmpF porin and to detect the electrostatic potential generated by the protein. To this end the OmpF porin trimers from Escherichia coli was...
Müller, Daniel J, Kessler, Max, Oesterhelt, Filipp, Möller, Clemens, Oesterhelt, Dieter, Gaub, Hermann
The combination of high-resolution atomic force microscopy imaging and single-molecule force spectroscopy allows the identification, selection, and mechanical investigation of individual proteins. In...
Molecular Force Modulation Spectroscopy Revealing the Dynamic Response of Single Bacteriorhodopsins
Janovjak, Harald, Müller, Daniel J., Humphris, Andrew D. L.
Recent advances in atomic force microscopy allowed globular and membrane proteins to be mechanically unfolded on a single-molecule level. Presented is an extension to the existing force spectroscopy...
Complex Stability of Single Proteins Explored by Forced Unfolding Experiments
Janovjak, Harald, Sapra, K. Tanuj, Müller, Daniel J.
In the last decade atomic force microscopy has been used to measure the mechanical stability of single proteins. These force spectroscopy experiments have shown that many water-soluble and membrane...