Cutaneous cancer stem cell maintenance is dependent on beta-catenin signalling. (2008)
Malanchi, Ilaria, Peinado, Hector, Kassen, Deepika, Hussenet, Thomas, Metzger, Daniel, Chambon, Pierre, ...
Continuous turnover of epithelia is ensured by the extensive self-renewal capacity of tissue-specific stem cells. Similarly, epithelial tumour maintenance relies on cancer stem cells (CSCs), which...
Terrand, Jérome, Bruban, Véronique, Zhou, Li, Gong, Wanfeng, El Asmar, Zeina, May, Petra, ...
The low-density lipoprotein receptor-related protein LRP1 is a cell surface receptor with functions in diverse physiological pathways, including lipid metabolism. Here we show that LRP1-deficient...
Calado, Joaquim, Sznajer, Yves, Metzger, Daniel, Rita, Ana, Hogan, Marie C., Kattamis, Antonis, ...
Introduction. Familial renal glucosuria (FRG) is a rare renal tubular disorder caused by mutations within the SLC5A2 gene. It is characterized by persistent glucosuria in the absence of...
IL-13 induces expression of CD36 in human monocytes through PPARgamma activation. (2007)
Berry, Antoine, Balard, Patricia, Coste, Agnès, Olagnier, David, Lagane, Céline, Authier, Hélène, ...
The class B scavenger receptor CD36 is a component of the pattern recognition receptors on monocytes that recognizes a variety of molecules. CD36 expression in monocytes depends on exposure to...
Coste, Agnes, Dubuquoy, Laurent, Barnouin, Romain, Annicotte, Jean-Sebastien, Magnier, Benjamin, Notti, Mario, ...
Liver receptor homolog-1 (LRH-1) is a nuclear receptor involved in intestinal lipid homeostasis and cell proliferation. Here we show that haploinsufficiency of LRH-1 predisposes mice to the...
Dali-Youcef, Nassim, Mataki, Chikage, Coste, Agnès, Messaddeq, Nadia, Giroud, Sylvain, Blanc, Stéphane, ...
The role of the tumor suppressor retinoblastoma protein (pRb) has been firmly established in the control of cell cycle, apoptosis, and differentiation. Recently, it was demonstrated that lack of pRb...
Mataki, Chikage, Magnier, Benjamin C, Houten, Sander M, Annicotte, Jean-Sébastien, Argmann, Carmen, Thomas, Charles, ...
Bile acids (BAs) are water-soluble end products from cholesterol metabolism and are essential for efficient absorption of dietary lipids. By using targeted somatic mutagenesis of the nuclear receptor...
Oskarsson, Thordur, Essers, Marieke Alida Gertruda, Dubois, Nicole, Offner, Sandra, Dubey, Christelle, Roger, Catherine, ...
The target gene(s) required for Myc-mediated tumorigenesis are still elusive. Here we show that while endogenous c-Myc is surprisingly dispensable for skin homeostasis and TPA-induced hyperplasia,...
Nenci, Arianna, Huth, Marion, Funteh, Alfred, Schmidt-Supprian, Marc, Bloch, Wilhelm, Metzger, Daniel, ...
NF-κB essential modulator (NEMO), the regulatory subunit of the IκB kinase, is essential for NF-κB activation. Mutations disrupting the X-linked NEMO gene cause incontinentia pigmenti (IP), a...
Calléja, Cécile, Messaddeq, Nadia, Chapellier, Benoit, Yang, Haiyuan, Krezel, Wojciech, Li, Mei, ...
Using genetic and pharmacological approaches, we demonstrate that both RARγ/RXRα heterodimers involved in repression events, as well as PPARβ(δ)/RXRα heterodimers involved in activation events,...
Nenci, Arianna, Huth, Marion, Funteh, Alfred, Schmidt-Supprian, Marc, Bloch, Wilhelm, Metzger, Daniel, ...
NF-κB Essential Modulator (NEMO), the regulatory subunit of the IκB kinase, is essential for NF-κB activation. Mutations disrupting the X-linked NEMO gene cause incontinentia pigmenti (IP), a...
Rousseaux, Christel, Lefebvre, Bruno, Dubuquoy, Laurent, Lefebvre, Philippe, Romano, Olivier, Auwerx, Johan, ...
5-aminosalicylic acid (5-ASA) is an antiinflammatory drug widely used in the treatment of inflammatory bowel diseases. It is known to inhibit the production of cytokines and inflammatory mediators,...
McLean, Gordon W., Komiyama, Noboru H., Serrels, Bryan, Asano, Hidefumi, Reynolds, Louise, Conti, Francesco, ...
We have generated mice with a floxed fak allele under the control of keratin-14-driven Cre fused to a modified estrogen receptor (CreERT2). 4-Hydroxy-tamoxifen treatment induced fak deletion in the...
Temporally Controlled Site-Specific Mutagenesis in the Germ Cell Lineage of the Mouse Testis1 (2003)
Philipp Weber, Michael Schuler, Christelle Gérard, Manuel Mark, Daniel Metzger, Pierre Chambon
We have obtained a PrP-Cre-ERT transgenic mouse line (28.8) that selectively expresses in testis the tamoxifen-inducible Cre-ERT recombinase under the control of a mouse Prion protein (PrP)...
Abu-Abed, Suzan, Dollé, Pascal, Metzger, Daniel, Beckett, Barbara, Chambon, Pierre, Petkovich, Martin
Verneuil, Hubert De, Metzger, Daniel
Using a transient co-transfection system we have demonstrated that response elements for estrogen (ER), thyroid hormone (TR) and retinoic acid receptors (RAR) are closely related. Thyroid...
Imai, Takeshi, Jiang, Ming, Chambon, Pierre, Metzger, Daniel
Retinoid X receptor α (RXRα) is involved in multiple signaling pathways, as a heterodimeric partner of several nuclear receptors. To investigate its function in energy homeostasis, we have...
Spatio-temporally controlled site-specific somatic mutagenesis in the mouse
Brocard, Jacques, Warot, Xavier, Wendling, Olivia, Messaddeq, Nadia, Vonesch, Jean-Luc, Chambon, Pierre, ...
The efficient introduction of somatic mutations in a given gene, at a given time, in a specific cell type will facilitate studies of gene function and the generation of animal models for human...
Vallier, Ludovic, Mancip, Jimmy, Markossian, Suzy, Lukaszewicz, Agnès, Dehay, Colette, Metzger, Daniel, ...
We have developed a universally applicable system for conditional gene expression in embryonic stem (ES) cells that relies on tamoxifen-dependent Cre recombinase-loxP site-mediated recombination and...
Imai, Takeshi, Jiang, Ming, Kastner, Philippe, Chambon, Pierre, Metzger, Daniel
Retinoid X receptors (RXRs) are involved in a number of signaling pathways as heterodimeric partners of numerous nuclear receptors. Hepatocytes express high levels of the RXRα isotype, as well as...
Endoh, Hideki, Maruyama, Kazunori, Masuhiro, Yoshikazu, Kobayashi, Yoko, Goto, Masahide, Tai, Hitoshi, ...
The estrogen receptor (ER) regulates the expression of target genes in a ligand-dependent manner. The ligand-dependent activation function AF-2 of the ER is located in the ligand binding domain...
Notch signaling is a direct determinant of keratinocyte growth arrest and entry into differentiation
Rangarajan, Annapoorni, Talora, Claudio, Okuyama, Ryuhei, Nicolas, Michael, Mammucari, Cristina, Oh, Heysun, ...
The role of Notch signaling in growth/differentiation control of mammalian epithelial cells is still poorly defined. We show that keratinocyte-specific deletion of the Notch1 gene results in marked...
Chapellier, Benoit, Mark, Manuel, Messaddeq, Nadia, Calléja, Cécile, Warot, Xavier, Brocard, Jacques, ...
To investigate the roles of retinoic acid (RA) receptors (RARs) in the physiology of epidermis that does not express RARβ, conditional spatio-temporally controlled somatic mutagenesis was used to...
TAF10 (TAFII30) Is Necessary for TFIID Stability and Early Embryogenesis in Mice
Mohan II, William S., Scheer, Elisabeth, Wendling, Olivia, Metzger, Daniel, Tora, Làszlò
TAF10 (formerly TAFII30), is a component of TFIID and the TATA box-binding protein (TBP)-free TAF-containing complexes (TFTC/PCAF/STAGA). To investigate the physiological function of TAF10, we...
Suppressive function of androgen receptor in bone resorption
Kawano, Hirotaka, Sato, Takashi, Yamada, Takashi, Matsumoto, Takahiro, Sekine, Keisuke, Watanabe, Tomoyuki, ...
As locally converted estrogen from testicular testosterone contributes to apparent androgen activity, the physiological significance of androgen receptor (AR) function in the beneficial effects of...
Brain masculinization requires androgen receptor function
Sato, Takashi, Matsumoto, Takahiro, Kawano, Hirotaka, Watanabe, Tomoyuki, Uematsu, Yoshikatsu, Sekine, Keisuke, ...
Testicular testosterone produced during a critical perinatal period is thought to masculinize and defeminize the male brain from the inherent feminization program and induce male-typical behaviors in...
Imai, Takeshi, Takakuwa, Reiko, Marchand, Sandra, Dentz, Emilie, Bornert, Jean-Marc, Messaddeq, Nadia, ...
The peroxisome proliferator-activated receptor γ (PPARγ) mediates the activity of the insulin-sensitizing thiazolidinediones and plays an important role in adipocyte differentiation and fat...
In vivo activation of PPAR target genes by RXR homodimers
IJpenberg, Annemieke, Tan, Nguan Soon, Gelman, Laurent, Kersten, Sander, Seydoux, Josiane, Xu, Jianming, ...
The ability of a retinoid X receptor (RXR) to heterodimerize with many nuclear receptors, including LXR, PPAR, NGF1B and RAR, underscores its pivotal role within the nuclear receptor superfamily....
McLean, Gordon W., Komiyama, Noboru H., Serrels, Bryan, Asano, Hidefumi, Reynolds, Louise, Conti, Francesco, ...
We have generated mice with a floxed fak allele under the control of keratin-14-driven Cre fused to a modified estrogen receptor (CreERT2). 4-Hydroxy-tamoxifen treatment induced fak deletion in the...
Abu-Abed, Suzan, Dollé, Pascal, Metzger, Daniel, Beckett, Barbara, Chambon, Pierre, Petkovich, Martin
The active derivative of vitamin A, retinoic acid (RA), is essential for normal embryonic development. The spatio-temporal distribution of embryonic RA results from regulated expression of...
Li, Mei, Messaddeq, Nadia, Teletin, Marius, Pasquali, Jean-Louis, Metzger, Daniel, Chambon, Pierre
To investigate the role of retinoid X receptors (RXRs) in epidermal homeostasis, we generated RXRαβep-/- somatic mutants in which both RXRα and RXRβ are selectively ablated in epidermal...
Premature ovarian failure in androgen receptor-deficient mice
Shiina, Hiroko, Matsumoto, Takahiro, Sato, Takashi, Igarashi, Katsuhide, Miyamoto, Junko, Takemasa, Sayuri, ...
Premature ovarian failure (POF) syndrome, an early decline of ovarian function in women, is frequently associated with X chromosome abnormalities ranging from various Xq deletions to complete loss of...
Calléja, Cécile, Messaddeq, Nadia, Chapellier, Benoit, Yang, Haiyuan, Krezel, Wojciech, Li, Mei, ...
Using genetic and pharmacological approaches, we demonstrate that both RARγ/RXRα heterodimers involved in repression events, as well as PPARβ(δ)/RXRα heterodimers involved in activation events,...
Imai, Takeshi, Jiang, Ming, Chambon, Pierre, Metzger, Daniel
Retinoid X receptor α (RXRα) is involved in multiple signaling pathways, as a heterodimeric partner of several nuclear receptors. To investigate its function in energy homeostasis, we have...
Spatio-temporally controlled site-specific somatic mutagenesis in the mouse
Brocard, Jacques, Warot, Xavier, Wendling, Olivia, Messaddeq, Nadia, Vonesch, Jean-Luc, Chambon, Pierre, ...
The efficient introduction of somatic mutations in a given gene, at a given time, in a specific cell type will facilitate studies of gene function and the generation of animal models for human...
Vallier, Ludovic, Mancip, Jimmy, Markossian, Suzy, Lukaszewicz, Agnès, Dehay, Colette, Metzger, Daniel, ...
We have developed a universally applicable system for conditional gene expression in embryonic stem (ES) cells that relies on tamoxifen-dependent Cre recombinase-loxP site-mediated recombination and...
Imai, Takeshi, Jiang, Ming, Kastner, Philippe, Chambon, Pierre, Metzger, Daniel
Retinoid X receptors (RXRs) are involved in a number of signaling pathways as heterodimeric partners of numerous nuclear receptors. Hepatocytes express high levels of the RXRα isotype, as well as...
Endoh, Hideki, Maruyama, Kazunori, Masuhiro, Yoshikazu, Kobayashi, Yoko, Goto, Masahide, Tai, Hitoshi, ...
The estrogen receptor (ER) regulates the expression of target genes in a ligand-dependent manner. The ligand-dependent activation function AF-2 of the ER is located in the ligand binding domain...
Notch signaling is a direct determinant of keratinocyte growth arrest and entry into differentiation
Rangarajan, Annapoorni, Talora, Claudio, Okuyama, Ryuhei, Nicolas, Michael, Mammucari, Cristina, Oh, Heysun, ...
The role of Notch signaling in growth/differentiation control of mammalian epithelial cells is still poorly defined. We show that keratinocyte-specific deletion of the Notch1 gene results in marked...
Chapellier, Benoit, Mark, Manuel, Messaddeq, Nadia, Calléja, Cécile, Warot, Xavier, Brocard, Jacques, ...
To investigate the roles of retinoic acid (RA) receptors (RARs) in the physiology of epidermis that does not express RARβ, conditional spatio-temporally controlled somatic mutagenesis was used to...
TAF10 (TAFII30) Is Necessary for TFIID Stability and Early Embryogenesis in Mice
Mohan II, William S., Scheer, Elisabeth, Wendling, Olivia, Metzger, Daniel, Tora, Làszlò
TAF10 (formerly TAFII30), is a component of TFIID and the TATA box-binding protein (TBP)-free TAF-containing complexes (TFTC/PCAF/STAGA). To investigate the physiological function of TAF10, we...
Suppressive function of androgen receptor in bone resorption
Kawano, Hirotaka, Sato, Takashi, Yamada, Takashi, Matsumoto, Takahiro, Sekine, Keisuke, Watanabe, Tomoyuki, ...
As locally converted estrogen from testicular testosterone contributes to apparent androgen activity, the physiological significance of androgen receptor (AR) function in the beneficial effects of...
Abu-Abed, Suzan, Dollé, Pascal, Metzger, Daniel, Beckett, Barbara, Chambon, Pierre, Petkovich, Martin
The active derivative of vitamin A, retinoic acid (RA), is essential for normal embryonic development. The spatio-temporal distribution of embryonic RA results from regulated expression of...
Brain masculinization requires androgen receptor function
Sato, Takashi, Matsumoto, Takahiro, Kawano, Hirotaka, Watanabe, Tomoyuki, Uematsu, Yoshikatsu, Sekine, Keisuke, ...
Testicular testosterone produced during a critical perinatal period is thought to masculinize and defeminize the male brain from the inherent feminization program and induce male-typical behaviors in...
Imai, Takeshi, Takakuwa, Reiko, Marchand, Sandra, Dentz, Emilie, Bornert, Jean-Marc, Messaddeq, Nadia, ...
The peroxisome proliferator-activated receptor γ (PPARγ) mediates the activity of the insulin-sensitizing thiazolidinediones and plays an important role in adipocyte differentiation and fat...
In vivo activation of PPAR target genes by RXR homodimers
IJpenberg, Annemieke, Tan, Nguan Soon, Gelman, Laurent, Kersten, Sander, Seydoux, Josiane, Xu, Jianming, ...
The ability of a retinoid X receptor (RXR) to heterodimerize with many nuclear receptors, including LXR, PPAR, NGF1B and RAR, underscores its pivotal role within the nuclear receptor superfamily....
McLean, Gordon W., Komiyama, Noboru H., Serrels, Bryan, Asano, Hidefumi, Reynolds, Louise, Conti, Francesco, ...
We have generated mice with a floxed fak allele under the control of keratin-14-driven Cre fused to a modified estrogen receptor (CreERT2). 4-Hydroxy-tamoxifen treatment induced fak deletion in the...
Li, Mei, Messaddeq, Nadia, Teletin, Marius, Pasquali, Jean-Louis, Metzger, Daniel, Chambon, Pierre
To investigate the role of retinoid X receptors (RXRs) in epidermal homeostasis, we generated RXRαβep-/- somatic mutants in which both RXRα and RXRβ are selectively ablated in epidermal...
Premature ovarian failure in androgen receptor-deficient mice
Shiina, Hiroko, Matsumoto, Takahiro, Sato, Takashi, Igarashi, Katsuhide, Miyamoto, Junko, Takemasa, Sayuri, ...
Premature ovarian failure (POF) syndrome, an early decline of ovarian function in women, is frequently associated with X chromosome abnormalities ranging from various Xq deletions to complete loss of...
Calléja, Cécile, Messaddeq, Nadia, Chapellier, Benoit, Yang, Haiyuan, Krezel, Wojciech, Li, Mei, ...
Using genetic and pharmacological approaches, we demonstrate that both RARγ/RXRα heterodimers involved in repression events, as well as PPARβ(δ)/RXRα heterodimers involved in activation events,...
Oskarsson, Thordur, Essers, Marieke Alida Gertruda, Dubois, Nicole, Offner, Sandra, Dubey, Christelle, Roger, Catherine, ...
The target gene(s) required for Myc-mediated tumorigenesis are still elusive. Here we show that while endogenous c-Myc is surprisingly dispensable for skin homeostasis and TPA-induced hyperplasia,...
Li, Mei, Hener, Pierre, Zhang, Zhikun, Kato, Shigeaki, Metzger, Daniel, Chambon, Pierre
We have demonstrated that cytokine thymic stromal lymphopoietin (TSLP), whose expression is rapidly induced upon keratinocyte-selective ablation of retinoid X receptors (RXRs) -α and -β in the...
Tang, Xiaoren, Metzger, Daniel, Leeman, Susan, Amar, Salomon
Previously we identified a transcription factor, LPS-Induced TNF-α Factor (LITAF), mediating inflammatory cytokine expression in LPS-induced processes. To characterize the role of LITAF in vivo, we...
Retinal Dystrophy Resulting from Ablation of RXRα in the Mouse Retinal Pigment Epithelium
Mori, Mikiro, Metzger, Daniel, Picaud, Serge, Hindelang, Colette, Simonutti, Manuel, Sahel, José, ...
Vitamin A (retinol) actions in eye development are mediated by retinoic acid receptors (RARs and RXRs). Using the Cre/loxP system, we have selectively ablated RXRα in the retinal pigment epithelium...
Hedgehog Signaling Regulates Sebaceous Gland Development
Allen, Mary, Grachtchouk, Marina, Sheng, Hong, Grachtchouk, Vladimir, Wang, Anna, Wei, Lebing, ...
Epithelial progenitor cells in skin give rise to multiple lineages, comprising the hair follicle, an associated sebaceous gland, and overlying epidermis; however, the signals that regulate sebocyte...
Dali-Youcef, Nassim, Mataki, Chikage, Coste, Agnès, Messaddeq, Nadia, Giroud, Sylvain, Blanc, Stéphane, ...
The role of the tumor suppressor retinoblastoma protein (pRb) has been firmly established in the control of cell cycle, apoptosis, and differentiation. Recently, it was demonstrated that lack of pRb...
LRH-1-mediated glucocorticoid synthesis in enterocytes protects against inflammatory bowel disease
Coste, Agnes, Dubuquoy, Laurent, Barnouin, Romain, Annicotte, Jean-Sebastien, Magnier, Benjamin, Notti, Mario, ...
Liver receptor homolog-1 (LRH-1) is a nuclear receptor involved in intestinal lipid homeostasis and cell proliferation. Here we show that haploinsufficiency of LRH-1 predisposes mice to the...
Rousseaux, Christel, Lefebvre, Bruno, Dubuquoy, Laurent, Lefebvre, Philippe, Romano, Olivier, Auwerx, Johan, ...
5-aminosalicylic acid (5-ASA) is an antiinflammatory drug widely used in the treatment of inflammatory bowel diseases. It is known to inhibit the production of cytokines and inflammatory mediators,...
Mataki, Chikage, Magnier, Benjamin C., Houten, Sander M., Annicotte, Jean-Sébastien, Argmann, Carmen, Thomas, Charles, ...
Bile acids (BAs) are water-soluble end products from cholesterol metabolism and are essential for efficient absorption of dietary lipids. By using targeted somatic mutagenesis of the nuclear receptor...
Chiba, Hideki, Clifford, John, Metzger, Daniel, Chambon, Pierre
We have generated F9 murine embryonal carcinoma cells in which either the retinoid X receptor (RXR)α and retinoic acid receptor (RAR)α genes or the RXRα and RARγ genes are knocked out, and...
Michalik, Liliane, Desvergne, Béatrice, Tan, Nguan Soon, Basu-Modak, Sharmila, Escher, Pascal, Rieusset, Jennifer, ...
We show here that the α, β, and γ isotypes of peroxisome proliferator–activated receptor (PPAR) are expressed in the mouse epidermis during fetal development and that they disappear...
Ratnacaram, Chandrahas Koumar, Teletin, Marius, Jiang, Ming, Meng, Xiangjun, Chambon, Pierre, Metzger, Daniel
Studies of prostate cancer pathogenesis and development of new therapies have been hampered by a lack of appropriate mouse models. We have generated PSA-Cre-ERT2 mice that express the...
Orengo, James P., Chambon, Pierre, Metzger, Daniel, Mosier, Dennis R., Snipes, G. Jackson, Cooper, Thomas A.
Severe skeletal muscle wasting is the most debilitating symptom experienced by individuals with myotonic dystrophy type 1 (DM1). We present a DM1 mouse model with inducible and skeletal...
Premature Aging in Skeletal Muscle Lacking Serum Response Factor
Lahoute, Charlotte, Sotiropoulos, Athanassia, Favier, Marilyne, Guillet-Deniau, Isabelle, Charvet, Claude, Ferry, Arnaud, ...
Aging is associated with a progressive loss of muscle mass, increased adiposity and fibrosis that leads to sarcopenia. At the molecular level, muscle aging is known to alter the expression of a...
Zhang, Zhikun, Hener, Pierre, Frossard, Nelly, Kato, Shigeaki, Metzger, Daniel, Li, Mei, ...
Atopic dermatitis (AD) is often the initial step in the “atopic march,” given that more than half of AD patients with moderate to severe AD develop asthma later in life. Both AD and asthma share...
Ctip2/Bcl11b controls ameloblast formation during mammalian odontogenesis
Golonzhka, Olga, Metzger, Daniel, Bornert, Jean-Marc, Bay, Brian K., Gross, Michael K., Kioussi, Chrissa, ...
The transcription factor Ctip2/Bcl11b plays essential roles in developmental processes of the immune and central nervous systems and skin. Here we show that Ctip2 also plays a key role in tooth...
Bourgo, Ryan J., Siddiqui, Hasan, Fox, Sejal, Solomon, David, Sansam, Courtney G., Yaniv, Moshe, ...
Switch (SWI)/sucrose nonfermentable (SNF) is an evolutionarily conserved complex with ATPase function, capable of regulating nucleosome position to alter transcriptional programs within the cell. It...