Kinetics and Energetics of the Translocation of Maltose Binding Protein Folding Mutants (2008)
Tomkiewicz, Danuta, Nouwen, Nico, Driessen, Arnold J.M.
Protein translocation in Escherichia coli is mediated by the translocase that, in its minimal form, comprises a protein-conducting pore (SecYEG) and a motor protein (SecA). The SecYEG complex forms a...
Pushing, pulling and trapping – Modes of motor protein supported protein translocation (2007)
Tomkiewicz, Danuta, Nouwen, Nico, Driessen, Arnold J.M.
Protein translocation across the cellular membranes is an ubiquitous and crucial activity of cells. This process is mediated by translocases that consist of a protein conducting channel and an...
Single molecule studies on protein translocation in Escherichia coli (2007)
Proteins are the working horses of the cell. They are responsible for many events such as building, regulation, locomotion and signaling. Proteins are mainly synthesized in the cytoplasm. Frequently,...
Direct Observation of Chaperone-Induced Changes in a Protein Folding Pathway (2007)
Bechtluft, Philipp, Tyreman, Matthew, Tomkiewicz, Danuta, Nouwen, Nico, Tepper, Harald L., ...
How chaperone interactions affect protein folding pathways is a central problem in biology. With the use of optical tweezers and all-atom molecular dynamics simulations, we studied the effect of...
Single molecule studies on protein translocation in Escherichia coli / (2007)
Proefschrift Rijksuniversiteit Groningen.
SecA Supports a Constant Rate of Preprotein Translocation (2006)
Tomkiewicz, Danuta, Nouwen, Nico, Leeuwen, Ruud Van, Tans, Sander, Driessen, Arnold J.M.
In Escherichia coli, secretory proteins (preproteins) are translocated across the cytoplasmic membrane by the Sec system composed of a protein-conducting channel, SecYEG, and an ATP-dependent motor...
Plantinga, Titia H., Does, Chris Van Der, Tomkiewicz, Danuta, Keulen, Geertje Van, Konings, Wil N., Driessen, Arnold J.M.
Binding-protein-dependent secondary transporters make up a unique transport protein family. They use a solute-binding protein in proton-motive-force-driven transport. Only a few systems have been...