Nutrition: Basis for Healthy Children and Mothers in Bangladesh (2008)
Faruque, A.S.G., Ahmed, A.M. Shamsir, Ahmed, Tahmeed, Hossain, Md. Iqbal, Roy, S.K., Alam, Nurul, ...
Recent data from the World Health Organization showed that about 60% of all deaths, occurring among children aged less than five years (under-five children) in developing countries, could be...
Hashizume, Masahiro, Armstrong, Ben, Hajat, Shakoor, Wagatsuma, Yukiko, Faruque, Abu SG, Hayashi, Taiichi, ...
Background We estimated the effects of rainfall and temperature on the number of non-cholera diarrhoea cases and identified population factors potentially affecting vulnerability to the effect of the...
Faruque, Abu S.G., Alam, Khorshed, Malek, Mohammad A., Khan, Mohammed G.Y., Ahmed, Sabeena, Saha, Debasish, ...
Mahbubur Rahman, Mahbubur, Shoma, Shereen, Rashid, Harunur, Arifeen, Shams El, Baqui, A.H., Siddique, A.K., ...
Antimicrobial resistance of Shigella isolates in Bangladesh, during 2001-2002, was studied and compared with that of 1991-1992 to identify the changes in resistance patterns and trends. A...
Faruque, Abu S.G., Alam, Khorshed, Malek, Mohammad A., Khan, Mohammed G.Y., Ahmed, Sabeena, Saha, Debasish, ...
Enhanced Isolation of Shigella Species by Extended Incubation of Primary Isolation Plates (2007)
Alam, Khorshed, Hosain, M. Anowar, Islam, Nazrul, Sen, Sunil Kumar, Sur, Gour Chandra, Kaisar A., Talukder, ...
Improved Indicators of Infant Mortality for Integrated Primary Healthcare Programmes (2007)
Sack, David A., Ahmed, S., Razzaque, Abdur, Chakraborty, J., Yunus, Mohammad
Mortality and fertility rates are decreasing rapidly in many developing countries. It is argued that the indices commonly used as measures of these changes, i.e. infant mortality rate and fertility...
Ansaruzzaman, M, Chowdhury, A, Bhuiyan, N.A, Safa, Ashrafus, Sultana, Marzia, Lucas, Marcelino, ...
US-Japan Cooperative medical Science Meeting
Azim, Tasnim, Chowdhury, Ezazul I, Reza, Masud, Ahmed, Munir, Uddin, Mohammed T, Khan, Repon, ...
Abstract Background: Very little is known about female injecting drug users (IDU) in Bangladesh but anecdotal evidence suggests that they are hidden and very vulnerable to HIV through both their...
Editorial- Herd Protection and Herd Amplification in Cholera (2006)
Recent analysis of results of the 1985 vaccine trial in Bangladesh showed that a killed oral cholera vaccine could provide herd protection (1), and this finding sheds new light on the potential...
Emch, Michael, Ali, Mohammad, Park, Jin-Kyung, Yunus, Mohammad, Sack, David A, Clemens, John D
Objectives The effectiveness of vaccines in populations must consider both direct and indirect protection. This study reanalyses data from a large individually randomized oral cholera vaccine trial...
Emch, Michael, Ali, Mohammad, Park, Jin-Kyung, Yunus, Mohammad, Sack, David A., Clemens, John D.
Objectives The effectiveness of vaccines in populations must consider both direct and indirect protection. This study reanalyses data from a large individually randomized oral cholera vaccine trial...
Detection and characterization of human group C rotaviruses in Bangladesh (2005)
Banik, Sukalyani, Faruque, Abu S G, Taniguchi, Koki, Sack, David A, ...
Group C rotaviruses were detected by reverse transcription-PCR in 14 (2.3%) of 611 group A rotavirus-negative stool specimens from the patients admitted to the International Centre for Diarrhoeal...
Characterization of a novel P[25],G11 human group A rotavirus (2005)
Nahar, Sharifun, Podder, Goutam, Sack, David A, Azim, Tasnim, ...
A novel rotavirus strain (Dhaka6) isolated from a 21-year-old Bangladeshi male patient was characterized by sequence analysis of its VP7 and VP4 gene segments. Phylogenetic analysis of the VP7 gene...
Rahman, Mustafizur, Sultana, Rasheda, Podder, Goutam, Faruque, Abu SG, Matthijnssens, Jelle, Zaman, Khalequz, ...
Abstract Background Rotavirus genotyping is performed by using reverse transcription PCR with type-specific-primers. Because the high rotavirus mutation rate generates an extensive genomic variation,...
Sultana, Rasheda, Podder, Goutam, Faruque, Abu S G, Zaman, Khalequz, ...
BACKGROUND: Rotavirus genotyping is performed by using reverse transcription PCR with type-specific-primers. Because the high rotavirus mutation rate generates an extensive genomic variation,...
Talukder, Kaisar A., Khajanchi, Bijay K., Aminul Islam, M., Dutta, Dilip K., Islam, Zhahirul, Safa, Ashrafus, ...
Objectives: The aim of the present study was to determine the clonal relationships of ciprofloxacin-resistant Shigella dysenteriae type 1 strains isolated from south Asia, and S. dysenteriae 1...
Talukder, Kaisar A., Khajanchi, Bijay K., Islam, M. Aminul, Dutta, Dilip K., Islam, Zhahirul, Safa, Ashrafus, ...
Objectives: The aim of the present study was to determine the clonal relationships of ciprofloxacin-resistant Shigella dysenteriae type 1 strains isolated from south Asia, and S. dysenteriae 1...
Talukder, Kaisar A., Dutta, Dilip K., Safa, Ashrafus, Ansaruzzaman, M., Hassan, Ferdaus, Alam, Khorshed, ...
Faruque, Shah M., Rahman, M. Mostafizur, Hasan, A.K.M., Nair, G. Balakrish, Mekalanos, John J., Sack, David A.
Toxigenic Vibrio cholerae strains are lysogens of CTX(Phi), a filamentous bacteriophage which encodes cholera toxin (CT). Following infection of recipient V. cholerae cells by CTX(Phi), the phage...
Faruque, Shah M., Saha, Manujendra N., Sack, David A., Sack, R. Bradley, Takeda, Yoshifumi, ...
Sixty-four representative strains of Vibrio cholerae O139 were analyzed, to re-examine the origin of this serogroup. Ribotyping differentiated the strains into 3 HindIII and 7 BglI ribotypes. One...
Sunlight-induced propagation of the lysogenic phage encoding cholera toxin (2000)
Faruque, Shah M., Rahman, M. Mostafizur, Waldor, Matthew K., Sack, David A.
In toxigenic Vibrio cholerae, the cholera enterotoxin (CT) is encoded by CTXPhi, a lysogenic bacteriophage. The propagation of this filamentous phage can result in the origination of new toxigenic...
Faruque, Shah M., Siddique, A.K., Saha, MN, Rahman, M. Mostafizur, Zaman, K., ...
Vibrio cholerae O139 Bengal initially appeared in the southern coastal region of Bangladesh and spread northward, causing explosive epidemics during 1992 and 1993. The resurgence of V. cholerae O139...
Clemens, John D., Rao, Malla R., Chakraborty, J., Yunus, Mohammad, Ali, Mohammed, Kay, Bradford, ...
Huq, Anwarul, Parveen, Salina, Qadri, Firdausi, Sack, David A., Colwell, Rita R.
Vibrio cholerae 01 strains of El Tor and Classical biotypes and Ogawa and Inaba serotypes were isolated from both patients and pond water, the latter used by the patients from whom the V. cholerae 01...
Clemens, John D., Rao, Malla, Sack, David A., Ahmed, Faruque, Chakraborty, J., ...
The authors estimated the incidence rates of cholera and death between 1985 and 1988 for 32,642 age- and sex-eligible persons who did not participate in a randomized, placebo-controlled field trial...
BREAST FEEDING AND THE RISK OF SEVERE CHOLERA IN RURAL BANGLADESHI CHILDREN (1990)
CLEMENS, JOHN D., SACK, DAVID A., HARRIS, JEFFREY R., KHAN, M. R., CHAKRABORTY, J., CHOWDHURY, SHAHRIAR, ...
The association between breast feeding and the risk of severe cholera was examined in a case-control study of rural Bangladeshi children under 36 months of age who were studied in 1985–1986 during...
CLEMENS, JOHN D, STANTON, BONITA F, HARRIS, JEFFREY R, CHAKRABORTY, J, SACK, DAVID A, RAO, MALLA R, ...
Clemens J D (International Centre for Diarrhoeal Disease Research, Bangladesh, Dhaka, Bangladesh), Stanton B F, Harris J R, Chakraborty J, Sack D A, Rao M R, Ahmed F, Ansaruzzaman M, Yunus M,...
Discontinuation of breast-feeding during episodes of diarrhoea in rural Bangladeshi children (1988)
Clemens, John D., Harris, Jeffery R., Sack, David A., Huda, Md. N., Chowdhury, Shahriar, Ali, Md, ...
Discontinuation of breast-feeding during an episode of childhood diarrhoea is widely regarded as a common, high-risk practice in the developing world. We studied cessation of breast-feeding in a...
Clemens, John D., Sack, David A., Harris, Jeffery R., Chakraborty, J., Neogy, P.K., Stanton, B., ...
The B subunit (BS) of cholera toxin and that of the heat-labile enterotoxin (LT) of enterotoxigenic Escherichia coli (ETEC) are antigenically similar. We therefore assessed whether a combined cholera...
Field trial of oral cholera vaccines in Bangladesh: results of one year of follow-up (1988)
Clemens, John D., Harris, Jeffery R., Sack, David A., Chakraborty, J., Ahmed, Faruque, Stanton, Bonita F., ...
We assessed the protective efficacy (PE) of three doses of B subunit-killed whole cell (BS-WC) and killed whole cell-only (WC) oral cholera vaccines in a randomized, double-blind trial among 62,285...
Clemens, John D., Sack, David A., Harris, Jeffery R., Chakraborty, J., Khan, M.R., Stanton, Bonita F., ...
The impact of B subunit killed whole-cell (BS-WC) and killed whole-cell-only (WC) oral cholera vaccines was assessed in a randomised double-blind trial in rural Bangladesh. 62,285 children aged 2-15...
Ahmed, Zia Uddin, Sarker, Mahfuz R., Haider, K., Chowdhury, Khorshed A., Sack, David A., Ciznar, Ivan
Epidemiology of persistent diarrhoea in Bangladeshi children (1987)
Baqui, Abdullah H., Yunus, Md., Fauveau, Vincent, Rahman, Mahbubur, Rowland, M.G.M., Sack, David A., ...
Clemens, John D., Stanton, Bonita F., Chakraborty, J., Sack, David A., Khan, M.R., Huda, S., ...
We conducted a randomized trial among persons in rural Bangladesh to evaluate the side effects and immunogenicity of orally administered B subunit-killed whole cell (BS-WC) and killed whole cell-only...
Field trial of oral cholera vaccines in Bangladesh (1986)
Clemens, John D., Sack, David A., Harris, Jeffery R., Chakraborty, J., Khan, M.R., Stanton, Bonita F., ...
The protective efficacy of oral B subunit killed whole-cell (BS-WC) and killed whole-cell (WC) cholera vaccines was assessed in 63 498 Bangladeshi children aged 2-15 years and women aged over 15...
Evaluation of safety and immunogenicity of oral rotavirus vaccine for use in Bangladesh (1985)
Harris, Jeffrey, Sack, David A., Chakraborty, J., Khan, M.R., Rowland, Michael G.M., Yunus, Md., ...
Islam, Md. Sirajul, Bradley, David J., Drasar, Bohumil S., Aziz, K.M.S., Huq, Md. Imdadul, Sack, David A.
Svennerholm, Ann-Mari, Jertborn, Marianne, Gothefors, Leif, Sack, David A., Holmgren, Jan
Mucosal and systemic immune responses to a new oral cholera vaccine, consisting of the B subunit plus killed vibrios, were studied in Bangladeshi volunteers and compared with those to clinical...
Rotavirus diarrhoea: an expanding clinical spectrum (1983)
Clemens, John D., Ahmed, Mosifuddin, Butler, Thomas, Greenough III, William B., Sack, David A., Stanton, Bonita F.
To explore the clinical spectrum of rotavirus diarrhoea in Bangladeshi children, we reviewed surveillance data from randomly selected diarrhoeal cases who were intensively studied at the...
Carbohydrate malabsorption in infants with rotavirus diarrhea (1982)
Sack, David A., Rhoads, Marc, Molla, Ayesha, Molla, A. Majid, Wahed, M. Abdul
We studied a group of patients with rotavirus diarrhea to determine the association of carbohydrate malabsorption during diarrhea with the degree of acidosis and severity of purging. Unlike...
Islam, M.R., Sack, David A., Holmgren, Jan, Bardhan, P.K., Rabbani, G.H.
Four hundred and ten patients with severe watery diarrhea; including 316 patients with cholera, were studied in a double-blind, randomized, placebo controlled trial to determine if chlorpromazine (1...
Molla, A. Majid, Rahman, Mizanur, Sarker, S. Alam, Sack, David A., Molla, Ayesha
One hundred twenty children below 5 years of age with diarrhea caused by Vibrio cholerae, enterotoxigenic Escherichia coli, or rotavirus were studied for stool electrolyte composition and purging...
Black, Robert E., Merson, Michael H., Taylor, Philip R., Yolken, Robert H., Sack, David A.
The use of oral rehydration solutions containing essential electrolytes and either glucose or sucrose of equal osmolality was compared in a double-blind sequential trial of 784 children with...
Sack, David A., Neogi, P.K.B., Alam, Md. Khorshed
We have developed an enzyme-linked immunosorbent assay for quantitating human immunoglobulin A in human secretions and serum. The procedure uses Immunobeads as the solid phase, has a sensitivity of...
Seroepidemiology of rotavirus infection in rural Bangladesh (1980)
Sack, David A., Gilman, Robert H., Kapikian, A.Z., Aziz, K.M.S.
A prospective seroepidemiological study of rotavirus infection was performed in children in a village in rural Bangladesh. Ninety-three percent of the children had detectable antibodies during the...
Sack, David A., Huda, S., Neogi, P.K.B., Daniel, Richard R., Spira, William M.
We have developed a microtiter enzyme-linked immunosorbent assay method for detecting the heat-labile enterotoxins of Vibrio cholerae and Escherichia coli using GM1 ganglioside as the base coat. This...
Sack, David A., Islam, Sirajul, Brown, Kenneth H., Islam, Asma, ...
We performed a double-blind trial comparing sucrose electrolyte oral solution with glucose electrolyte oral solution in children less than 5 years of age with severe cholera-like diarrhea. Of 111...
Prevalence of antibody to the Norwalk virus in various countries (1979)
Greenberg, Harry B., Valdesuso, Jose, Kapikian, Albert Z., Chanock, Robert M., Wyatt, Richard G., Szmuness, Wyatt Wolf, ...
Serum samples from children and adults from several countries were tested by radioimmunoassay for antibody to the Norwalk virus. Antibody was commonly found in adults from all the countries tested....
Greenberg, Harry B., Levine, Myron M., Merson, Michael H., Sack, R. Bradley, Sack, David A., Valdesuso, Jose R., ...
The development of a solid-phase microtiter radioimmunoassay blocking test to detect serum antibody to Escherichia coli heat-labile enterotoxin is described. The assay is easy to perform and...
Sack, David A., Chowdhury, A.M., Eusof, Abu, Ali, Md. Akbar, Merson, Michael H., Islam, Sirajul, ...
Enterotoxigenic Escherichia coli isolated from patients at a hospital in Dacca (1977)
Sack, David A., McLaughlin, James C., Sack, R. Bradely, Orskov, Frits, Orskov, Ida
Enterotoxigenic strains of Escherichia coli, a significant cause of acute, watery, cholera-like diarrhea, were isolated from 23 of 65 patients with diarrhea in whom no other enteric pathogens were...
Enterotoxigenic Escherichia coli and Reovirus-like agent in rural Bangladesh (1976)
Ryder, Robert W., Sack, David A., Kapikian, Albert Z., McLaughlin, James C., Chakraborty, Jyotsnamony, Rahman, A.S. Mizanur, ...
48 patients admitted to a rural Bangladesh hospital with dehydration secondary to diarrhea were examined for infection caused by (R.L.A.) reovirus-like agent or (E.T.E.C.) enterotoxigenic Escherichia...
Faruque, Shah M., Siddique, A. K., Saha, Manujendra N., Rahman, M. Mostafizur, Zaman, K., ...
Vibrio cholerae O139 Bengal initially appeared in the southern coastal region of Bangladesh and spread northward, causing explosive epidemics during 1992 and 1993. The resurgence of V. cholerae O139...
Talukder, Kaisar A., Dutta, Dilip K., Safa, Ashrafus, Ansaruzzaman, M., Hassan, Ferdaus, Alam, Khorshed, ...
Of 469 recently isolated Shigella flexneri strains, 452 agglutinated with Shigella flexneri-specific monoclonal antibodies. Of these, 396 could be assigned to 10 of the currently recognized 15...
Chang, H. Sunny, Sack, David A.
We describe here a novel method for measuring in vitro antibody secretion from the tissue culture of human B lymphocytes in peripheral blood mononuclear cells (PBMC) after oral vaccination with a...
Sunlight-Induced Propagation of the Lysogenic Phage Encoding Cholera Toxin
Faruque, Shah M., Rahman, M. Mostafizur, Waldor, Matthew K., Sack, David A.
In toxigenic Vibrio cholerae, the cholera enterotoxin (CT) is encoded by CTXΦ, a lysogenic bacteriophage. The propagation of this filamentous phage can result in the origination of new toxigenic...
Turner, Arthur K., Terry, Tamsin D., Sack, David A., Londoño-Arcila, Patricia, Darsley, Michael J.
Enterotoxigenic Escherichia coli (ETEC) is a leading cause of diarrhea in travelers to countries where the disease is endemic and causes a major disease burden in the indigenous population,...
Faruque, Shah M., Rahman, M. Mostafizur, Nair, G. Balakrish, Mekalanos, John J., Sack, David A.
Toxigenic Vibrio cholerae strains are lysogens of CTXΦ, a filamentous bacteriophage which encodes cholera toxin (CT). Following infection of recipient V. cholerae cells by CTXΦ, the phage genome...
Qadri, Firdausi, Asaduzzaman, Muhammad, Wennerås, Christine, Mohi, Golam, Albert, M. John, Abdus Salam, Mohammad, ...
Cholera toxin (CT)-specific antibody responses of the immunoglobulin E (IgE) isotype in the sera of adult patients suffering from infection with either Vibrio cholerae O1, V. cholerae O139, or...
Sack, David A., Lastovica, Albert J., Chang, Sunny H., Pazzaglia, Gary
Campylobacter jejuni with Gm1 ganglioside in the core of its lipopolysaccharide has been associated with Guillain-Barré syndrome. Since this epitope may be of considerable pathophysiologic...
Validation of a Volunteer Model of Cholera with Frozen Bacteria as the Challenge
Sack, David A., Tacket, Carol O., Cohen, Mitchell B., Sack, R. Bradley, Losonsky, Genevieve A., Shimko, Janet, ...
To evaluate a standardized inoculum of Vibrio cholerae for volunteer challenge studies, 40 healthy adult volunteers were challenged at three different institutions with a standard inoculum prepared...
Bhuiyan, N. A., Ansaruzzaman, M., Kamruzzaman, M., Alam, Khorshed, Chowdhury, N. R., Nishibuchi, M., ...
Sixty-six strains of Vibrio parahaemolyticus belonging to 14 serotypes were isolated from hospitalized patients in Dhaka, Bangladesh, from January 1998 to December 2000. Among these, 48 strains...
Talukder, Kaisar A., Islam, M. Aminul, Dutta, Dilip K., Hassan, Ferdaus, Safa, Ashrafus, Nair, G. B., ...
Twenty-one atypical Shigella flexneri type 4 strains isolated from patients attending the Dhaka treatment center of the International Centre for Diarrhoeal Disease Research, Bangladesh, were...
Faruque, Shah M., Khan, Rasel, Kamruzzaman, M., Yamasaki, Shinji, Ahmad, Q. Shafi, Azim, Tasnim, ...
Bacillary dysentery caused by Shigella species is a public health problem in developing countries including Bangladesh. Although, shigellae-contaminated food and drinks are often the source of the...
Faruque, Shah M., Kamruzzaman, M., Nandi, Ranjan K., Ghosh, A. N., Nair, G. Balakrish, ...
In toxigenic Vibrio cholerae, cholera toxin is encoded by the CTX prophage, which consists of a core region carrying ctxAB genes and genes required for CTXΦ morphogenesis, and an RS2 region encoding...
Nair, G. Balakrish, Faruque, Shah M., Bhuiyan, N. A., Kamruzzaman, M., Siddique, A. K., Sack, David A.
The sixth pandemic of cholera and, presumably, the earlier pandemics were caused by the classical biotype of Vibrio cholerae O1, which was progressively replaced by the El Tor biotype representing...
Faruque, Shah M., Kamruzzaman, M., Meraj, Ismail M., Chowdhury, Nityananda, Nair, G. Balakrish, Sack, R. Bradley, ...
The major virulence factors of toxigenic Vibrio cholerae are cholera toxin (CT), which is encoded by a lysogenic bacteriophage (CTXΦ), and toxin-coregulated pilus (TCP), an essential colonization...
Talukder, Kaisar A., Islam, Zhahirul, Aminul Islam, M., Dutta, Dilip K., Safa, Ashrafus, Ansaruzzaman, M., ...
The serotypes of 144 strains of Shigella flexneri serotype 1 (serotypes 1a, 1b, and 1c) isolated from patients attending the Dhaka treatment center of the International Centre for Diarrhoeal Disease...
Qadri, Firdausi, Ryan, Edward T., Faruque, A. S. G., Ahmed, Firoz, Islam Khan, Ashraful, Islam, M. Monirul, ...
Gut-derived lymphocytes transiently migrate through the peripheral circulation before homing back to mucosal sites and can be detected using an ELISPOT-based antibody secreting cell (ASC) assay....
Bhuiyan, N. A., Qadri, Firdausi, Faruque, A. S. G., Malek, M. A, Salam, M. A., Nato, Farida, ...
We evaluated the recently developed dipsticks for the rapid detection of Vibrio cholerae serotypes O1 and O139 from rectal swabs of hospitalized diarrheal patients after enrichment for 4 h in...
Talukder, Kaisar A., Islam, M. Aminul, Khajanchi, Bijay K., Dutta, Dilip K., Islam, Zhahirul, Safa, Ashrafus, ...
A total of 358 Shigella dysenteriae strains isolated from patients attending the Dhaka treatment center of the International Centre for Diarrheal Disease Research, Bangladesh, between the years 1999...
Greenberg, Harry B., Levine, Myron M., Merson, Michael H., Sack, R. Bradley, Sack, David A., Valdesuso, Jose R., ...
The development of a solid-phase microtiter radioimmunoassay blocking test to detect serum antibody to Escherichia coli heat-labile enterotoxin is described. The assay is easy to perform and...
Reduction of cholera in Bangladeshi villages by simple filtration
Colwell, Rita R., Huq, Anwar, Islam, M. Sirajul, Aziz, K. M. A., Yunus, M., Khan, N. Huda, ...
Based on results of ecological studies demonstrating that Vibrio cholerae, the etiological agent of epidemic cholera, is commensal to zooplankton, notably copepods, a simple filtration procedure was...
CTXΦ-independent production of the RS1 satellite phage by Vibrio cholerae
Faruque, Shah M., Kamruzzaman, M., Sack, David A., Mekalanos, John J., Nair, G. Balakrish
The cholera toxin genes of Vibrio cholerae are encoded by the filamentous phage, CTXΦ. Chromosomal CTXΦ prophage DNA is often found flanked by copies of a related genetic element designated RS1,...
Emergence and evolution of Vibrio cholerae O139
Faruque, Shah M., Sack, David A., Sack, R. Bradley, Colwell, Rita R., Takeda, Yoshifumi, Nair, G. Balakrish
The emergence of Vibrio cholerae O139 Bengal during 1992–1993 was associated with large epidemics of cholera in India and Bangladesh and, initially, with a total displacement of the existing V....
Shigella dysenteriae Type 1-Specific Bacteriophage from Environmental Waters in Bangladesh
Faruque, Shah M., Chowdhury, Nityananda, Khan, Rasel, Hasan, M. Rubayet, Nahar, Jebun, Islam, M. Johirul, ...
Shigella dysenteriae type 1 is the causative agent of the most severe form of bacillary dysentery, which occurs as epidemics in many developing countries. We isolated a bacteriophage from surface...
Single-Dose Doxycycline for Cholera
Sack, David A., Islam, Sirajul, Rabbani, Hassan, Islam, Asma
To determine the efficacy of single-dose doxycycline in the treatment of cholera, we carried out a randomized prospective trial in 65 patients. Treatment consisted of either a single dose of 200 mg...
Faruque, Shah M., Chowdhury, Nityananda, Kamruzzaman, M., Dziejman, Michelle, Rahman, M. Hasibur, Sack, David A., ...
To understand the evolutionary events and possible selection mechanisms involved in the emergence of pathogenic Vibrio cholerae, we analyzed diverse strains of V. cholerae isolated from environmental...
Raqib, Rubhana, Kamal, S. M. Mostafa, Rahman, M. Jubayer, Rahim, Zeaur, Banu, Sayera, Bardhan, Pradip K., ...
We have previously demonstrated that Mycobacterium bovis BCG-specific immunoglobulin G antibodies in lymphocyte secretions (ALS) can be employed as a marker for active tuberculosis (TB). We aimed to...
Nusrin, Suraia, Khan, G. Yeahia, Bhuiyan, N. A., Ansaruzzaman, M., Hossain, M. A., Safa, Ashrafus, ...
PCR surveillance of the rstR genes of CTX phages in Vibrio cholerae O1 and O139 showed no relationship between the incidence of disease and changes in the rstR but showed variations in their presence...
Faruque, Shah M., Naser, Iftekhar Bin, Islam, M. Johirul, Faruque, A. S. G., Ghosh, A. N., Nair, G. Balakrish, ...
The relationship among (i) the local incidence of cholera, (ii) the prevalence in the aquatic environment of Vibrio cholerae, and (iii) bacterial viruses that attack potentially virulent O1 and O139...
Rahman, Mustafizur, Sultana, Rasheda, Podder, Goutam, Faruque, Abu SG, Matthijnssens, Jelle, Zaman, Khalequz, ...
Safa, Ashrafus, Bhuiyan, Nurul Amin, Alam, Munirul, Sack, David A., Nair, G. Balakrish
The genomes of the recently described Matlab variants of Vibrio cholerae O1 that are hybrids between classical and El Tor biotypes were compared with those of El Tor and classical biotypes by the use...
Self-limiting nature of seasonal cholera epidemics: Role of host-mediated amplification of phage
Faruque, Shah M., Islam, M. Johirul, Ahmad, Qazi Shafi, Faruque, A. S. G., Sack, David A., Nair, G. Balakrish, ...
Phage predation of Vibrio cholerae has recently been reported to be a factor that influences seasonal epidemics of cholera in Bangladesh. To understand more about this phenomenon, we studied the...
Ansaruzzaman, M., Lucas, Marcelino, Deen, Jacqueline L., Bhuiyan, N. A., Wang, Xuan-Yi, Safa, Ashrafus, ...
Forty-two episodes of Vibrio parahaemolyticus infections were detected in Beira, Mozambique, from January to May 2004. The majority of the isolates (81%) belonged to the pandemic serovars (O3:K6 and...
Characterization of a Novel P[25],G11 Human Group A Rotavirus
Rahman, Mustafizur, Matthijnssens, Jelle, Nahar, Sharifun, Podder, Goutam, Sack, David A., Azim, Tasnim, ...
A novel rotavirus strain (Dhaka6) isolated from a 21-year-old Bangladeshi male patient was characterized by sequence analysis of its VP7 and VP4 gene segments. Phylogenetic analysis of the VP7 gene...
Critical Factors Influencing the Occurrence of Vibrio cholerae in the Environment of Bangladesh
Huq, Anwar, Sack, R. Bradley, Nizam, Azhar, Longini, Ira M., Nair, G. Balakrish, Ali, Afsar, ...
The occurrence of outbreaks of cholera in Africa in 1970 and in Latin America in 1991, mainly in coastal communities, and the appearance of the new serotype Vibrio cholerae O139 in India and...
Qadri, Firdausi, Svennerholm, Ann-Mari, Shamsuzzaman, Sohel, Bhuiyan, Taufiqur Rahman, Harris, Jason B., Ghosh, A. N., ...
Vibrio cholerae O139 emerged in 1992 as a major cause of epidemic cholera. However, the incidence of disease due to this new serogroup subsequently decreased for almost a decade. In April 2002, there...
Detection and Characterization of Human Group C Rotaviruses in Bangladesh
Rahman, Mustafizur, Banik, Sukalyani, Faruque, Abu S. G., Taniguchi, Koki, Sack, David A., Van Ranst, Marc, ...
Group C rotaviruses were detected by reverse transcription-PCR in 14 (2.3%) of 611 group A rotavirus-negative stool specimens from the patients admitted to the International Centre for Diarrhoeal...
Blood Group, Immunity, and Risk of Infection with Vibrio cholerae in an Area of Endemicity
Harris, Jason B., Khan, Ashraful I., LaRocque, Regina C., Dorer, David J., Chowdhury, Fahima, Faruque, Abu S. G., ...
Individuals with blood group O are more susceptible than other individuals to severe cholera, although the mechanism underlying this association is unknown. To assess the respective roles of both...
McKenzie, Robin, Bourgeois, A. Louis, Engstrom, Fayette, Hall, Eric, Chang, H. Sunny, Gomes, Joseph G., ...
A vaccine against enterotoxigenic Escherichia coli (ETEC) is needed to prevent diarrheal illness among children in developing countries and at-risk travelers. Two live attenuated ETEC strains, PTL002...
Alam, Munirul, Sadique, Abdus, Bhuiyan, Nurul A., Nair, G. Balakrish, Siddique, A. K., ...
It has long been assumed that prolonged holding of environmental samples at the ambient air temperature prior to bacteriological analysis is detrimental to isolation and detection of Vibrio cholerae,...
Toxigenic Vibrio cholerae in the Aquatic Environment of Mathbaria, Bangladesh
Alam, Munirul, Sultana, Marzia, Nair, G. Balakrish, Sack, R. Bradley, Sack, David A., Siddique, A. K., ...
Toxigenic Vibrio cholerae, rarely isolated from the aquatic environment between cholera epidemics, can be detected in what is now understood to be a dormant stage, i.e., viable but nonculturable when...
Faruque, Shah M., Biswas, Kuntal, Udden, S. M. Nashir, Ahmad, Qazi Shafi, Sack, David A., Nair, G. Balakrish, ...
The factors that enhance the waterborne spread of bacterial epidemics and sustain the epidemic strain in nature are unclear. Although the epidemic diarrheal disease cholera is known to be transmitted...
Alam, Munirul, Hasan, Nur A., Sadique, Abdus, Bhuiyan, N. A., Ahmed, Kabir U., Nusrin, Suraia, ...
Since Vibrio cholerae O139 first appeared in 1992, both O1 El Tor and O139 have been recognized as the epidemic serogroups, although their geographic distribution, endemicity, and reservoir are not...
Raqib, Rubhana, Sarker, Protim, Bergman, Peter, Ara, Gul, Lindh, Monica, Sack, David A., ...
Shigella is a major cause of morbidity, mortality, and growth retardation for children in developing countries. Emergence of antibiotic resistance among Shigellae demands the development of effective...
Faruque, Shah M., Siddique, A. K., Saha, Manujendra N., Rahman, M. Mostafizur, Zaman, K., ...
Vibrio cholerae O139 Bengal initially appeared in the southern coastal region of Bangladesh and spread northward, causing explosive epidemics during 1992 and 1993. The resurgence of V. cholerae O139...
Talukder, Kaisar A., Dutta, Dilip K., Safa, Ashrafus, Ansaruzzaman, M., Hassan, Ferdaus, Alam, Khorshed, ...
Of 469 recently isolated Shigella flexneri strains, 452 agglutinated with Shigella flexneri-specific monoclonal antibodies. Of these, 396 could be assigned to 10 of the currently recognized 15...
Chang, H. Sunny, Sack, David A.
We describe here a novel method for measuring in vitro antibody secretion from the tissue culture of human B lymphocytes in peripheral blood mononuclear cells (PBMC) after oral vaccination with a...
Sunlight-Induced Propagation of the Lysogenic Phage Encoding Cholera Toxin
Faruque, Shah M., Rahman, M. Mostafizur, Waldor, Matthew K., Sack, David A.
In toxigenic Vibrio cholerae, the cholera enterotoxin (CT) is encoded by CTXΦ, a lysogenic bacteriophage. The propagation of this filamentous phage can result in the origination of new toxigenic...
Turner, Arthur K., Terry, Tamsin D., Sack, David A., Londoño-Arcila, Patricia, Darsley, Michael J.
Enterotoxigenic Escherichia coli (ETEC) is a leading cause of diarrhea in travelers to countries where the disease is endemic and causes a major disease burden in the indigenous population,...
Faruque, Shah M., Rahman, M. Mostafizur, Nair, G. Balakrish, Mekalanos, John J., Sack, David A.
Toxigenic Vibrio cholerae strains are lysogens of CTXΦ, a filamentous bacteriophage which encodes cholera toxin (CT). Following infection of recipient V. cholerae cells by CTXΦ, the phage genome...
Qadri, Firdausi, Asaduzzaman, Muhammad, Wennerås, Christine, Mohi, Golam, Albert, M. John, Abdus Salam, Mohammad, ...
Cholera toxin (CT)-specific antibody responses of the immunoglobulin E (IgE) isotype in the sera of adult patients suffering from infection with either Vibrio cholerae O1, V. cholerae O139, or...
Sack, David A., Lastovica, Albert J., Chang, Sunny H., Pazzaglia, Gary
Campylobacter jejuni with Gm1 ganglioside in the core of its lipopolysaccharide has been associated with Guillain-Barré syndrome. Since this epitope may be of considerable pathophysiologic...
Validation of a Volunteer Model of Cholera with Frozen Bacteria as the Challenge
Sack, David A., Tacket, Carol O., Cohen, Mitchell B., Sack, R. Bradley, Losonsky, Genevieve A., Shimko, Janet, ...
To evaluate a standardized inoculum of Vibrio cholerae for volunteer challenge studies, 40 healthy adult volunteers were challenged at three different institutions with a standard inoculum prepared...
Bhuiyan, N. A., Ansaruzzaman, M., Kamruzzaman, M., Alam, Khorshed, Chowdhury, N. R., Nishibuchi, M., ...
Sixty-six strains of Vibrio parahaemolyticus belonging to 14 serotypes were isolated from hospitalized patients in Dhaka, Bangladesh, from January 1998 to December 2000. Among these, 48 strains...
Talukder, Kaisar A., Islam, M. Aminul, Dutta, Dilip K., Hassan, Ferdaus, Safa, Ashrafus, Nair, G. B., ...
Twenty-one atypical Shigella flexneri type 4 strains isolated from patients attending the Dhaka treatment center of the International Centre for Diarrhoeal Disease Research, Bangladesh, were...
Faruque, Shah M., Khan, Rasel, Kamruzzaman, M., Yamasaki, Shinji, Ahmad, Q. Shafi, Azim, Tasnim, ...
Bacillary dysentery caused by Shigella species is a public health problem in developing countries including Bangladesh. Although, shigellae-contaminated food and drinks are often the source of the...
Faruque, Shah M., Kamruzzaman, M., Nandi, Ranjan K., Ghosh, A. N., Nair, G. Balakrish, ...
In toxigenic Vibrio cholerae, cholera toxin is encoded by the CTX prophage, which consists of a core region carrying ctxAB genes and genes required for CTXΦ morphogenesis, and an RS2 region encoding...
Nair, G. Balakrish, Faruque, Shah M., Bhuiyan, N. A., Kamruzzaman, M., Siddique, A. K., Sack, David A.
The sixth pandemic of cholera and, presumably, the earlier pandemics were caused by the classical biotype of Vibrio cholerae O1, which was progressively replaced by the El Tor biotype representing...
Faruque, Shah M., Kamruzzaman, M., Meraj, Ismail M., Chowdhury, Nityananda, Nair, G. Balakrish, Sack, R. Bradley, ...
The major virulence factors of toxigenic Vibrio cholerae are cholera toxin (CT), which is encoded by a lysogenic bacteriophage (CTXΦ), and toxin-coregulated pilus (TCP), an essential colonization...
Talukder, Kaisar A., Islam, Zhahirul, Aminul Islam, M., Dutta, Dilip K., Safa, Ashrafus, Ansaruzzaman, M., ...
The serotypes of 144 strains of Shigella flexneri serotype 1 (serotypes 1a, 1b, and 1c) isolated from patients attending the Dhaka treatment center of the International Centre for Diarrhoeal Disease...
Qadri, Firdausi, Ryan, Edward T., Faruque, A. S. G., Ahmed, Firoz, Islam Khan, Ashraful, Islam, M. Monirul, ...
Gut-derived lymphocytes transiently migrate through the peripheral circulation before homing back to mucosal sites and can be detected using an ELISPOT-based antibody secreting cell (ASC) assay....
Bhuiyan, N. A., Qadri, Firdausi, Faruque, A. S. G., Malek, M. A, Salam, M. A., Nato, Farida, ...
We evaluated the recently developed dipsticks for the rapid detection of Vibrio cholerae serotypes O1 and O139 from rectal swabs of hospitalized diarrheal patients after enrichment for 4 h in...
Talukder, Kaisar A., Islam, M. Aminul, Khajanchi, Bijay K., Dutta, Dilip K., Islam, Zhahirul, Safa, Ashrafus, ...
A total of 358 Shigella dysenteriae strains isolated from patients attending the Dhaka treatment center of the International Centre for Diarrheal Disease Research, Bangladesh, between the years 1999...
Greenberg, Harry B., Levine, Myron M., Merson, Michael H., Sack, R. Bradley, Sack, David A., Valdesuso, Jose R., ...
The development of a solid-phase microtiter radioimmunoassay blocking test to detect serum antibody to Escherichia coli heat-labile enterotoxin is described. The assay is easy to perform and...
Reduction of cholera in Bangladeshi villages by simple filtration
Colwell, Rita R., Huq, Anwar, Islam, M. Sirajul, Aziz, K. M. A., Yunus, M., Khan, N. Huda, ...
Based on results of ecological studies demonstrating that Vibrio cholerae, the etiological agent of epidemic cholera, is commensal to zooplankton, notably copepods, a simple filtration procedure was...
CTXΦ-independent production of the RS1 satellite phage by Vibrio cholerae
Faruque, Shah M., Kamruzzaman, M., Sack, David A., Mekalanos, John J., Nair, G. Balakrish
The cholera toxin genes of Vibrio cholerae are encoded by the filamentous phage, CTXΦ. Chromosomal CTXΦ prophage DNA is often found flanked by copies of a related genetic element designated RS1,...
Emergence and evolution of Vibrio cholerae O139
Faruque, Shah M., Sack, David A., Sack, R. Bradley, Colwell, Rita R., Takeda, Yoshifumi, Nair, G. Balakrish
The emergence of Vibrio cholerae O139 Bengal during 1992–1993 was associated with large epidemics of cholera in India and Bangladesh and, initially, with a total displacement of the existing V....
Shigella dysenteriae Type 1-Specific Bacteriophage from Environmental Waters in Bangladesh
Faruque, Shah M., Chowdhury, Nityananda, Khan, Rasel, Hasan, M. Rubayet, Nahar, Jebun, Islam, M. Johirul, ...
Shigella dysenteriae type 1 is the causative agent of the most severe form of bacillary dysentery, which occurs as epidemics in many developing countries. We isolated a bacteriophage from surface...
Single-Dose Doxycycline for Cholera
Sack, David A., Islam, Sirajul, Rabbani, Hassan, Islam, Asma
To determine the efficacy of single-dose doxycycline in the treatment of cholera, we carried out a randomized prospective trial in 65 patients. Treatment consisted of either a single dose of 200 mg...
Faruque, Shah M., Chowdhury, Nityananda, Kamruzzaman, M., Dziejman, Michelle, Rahman, M. Hasibur, Sack, David A., ...
To understand the evolutionary events and possible selection mechanisms involved in the emergence of pathogenic Vibrio cholerae, we analyzed diverse strains of V. cholerae isolated from environmental...
Raqib, Rubhana, Kamal, S. M. Mostafa, Rahman, M. Jubayer, Rahim, Zeaur, Banu, Sayera, Bardhan, Pradip K., ...
We have previously demonstrated that Mycobacterium bovis BCG-specific immunoglobulin G antibodies in lymphocyte secretions (ALS) can be employed as a marker for active tuberculosis (TB). We aimed to...
Nusrin, Suraia, Khan, G. Yeahia, Bhuiyan, N. A., Ansaruzzaman, M., Hossain, M. A., Safa, Ashrafus, ...
PCR surveillance of the rstR genes of CTX phages in Vibrio cholerae O1 and O139 showed no relationship between the incidence of disease and changes in the rstR but showed variations in their presence...
Faruque, Shah M., Naser, Iftekhar Bin, Islam, M. Johirul, Faruque, A. S. G., Ghosh, A. N., Nair, G. Balakrish, ...
The relationship among (i) the local incidence of cholera, (ii) the prevalence in the aquatic environment of Vibrio cholerae, and (iii) bacterial viruses that attack potentially virulent O1 and O139...
Rahman, Mustafizur, Sultana, Rasheda, Podder, Goutam, Faruque, Abu SG, Matthijnssens, Jelle, Zaman, Khalequz, ...
Safa, Ashrafus, Bhuiyan, Nurul Amin, Alam, Munirul, Sack, David A., Nair, G. Balakrish
The genomes of the recently described Matlab variants of Vibrio cholerae O1 that are hybrids between classical and El Tor biotypes were compared with those of El Tor and classical biotypes by the use...
Self-limiting nature of seasonal cholera epidemics: Role of host-mediated amplification of phage
Faruque, Shah M., Islam, M. Johirul, Ahmad, Qazi Shafi, Faruque, A. S. G., Sack, David A., Nair, G. Balakrish, ...
Phage predation of Vibrio cholerae has recently been reported to be a factor that influences seasonal epidemics of cholera in Bangladesh. To understand more about this phenomenon, we studied the...
Ansaruzzaman, M., Lucas, Marcelino, Deen, Jacqueline L., Bhuiyan, N. A., Wang, Xuan-Yi, Safa, Ashrafus, ...
Forty-two episodes of Vibrio parahaemolyticus infections were detected in Beira, Mozambique, from January to May 2004. The majority of the isolates (81%) belonged to the pandemic serovars (O3:K6 and...
Characterization of a Novel P[25],G11 Human Group A Rotavirus
Rahman, Mustafizur, Matthijnssens, Jelle, Nahar, Sharifun, Podder, Goutam, Sack, David A., Azim, Tasnim, ...
A novel rotavirus strain (Dhaka6) isolated from a 21-year-old Bangladeshi male patient was characterized by sequence analysis of its VP7 and VP4 gene segments. Phylogenetic analysis of the VP7 gene...
Critical Factors Influencing the Occurrence of Vibrio cholerae in the Environment of Bangladesh
Huq, Anwar, Sack, R. Bradley, Nizam, Azhar, Longini, Ira M., Nair, G. Balakrish, Ali, Afsar, ...
The occurrence of outbreaks of cholera in Africa in 1970 and in Latin America in 1991, mainly in coastal communities, and the appearance of the new serotype Vibrio cholerae O139 in India and...
Qadri, Firdausi, Svennerholm, Ann-Mari, Shamsuzzaman, Sohel, Bhuiyan, Taufiqur Rahman, Harris, Jason B., Ghosh, A. N., ...
Vibrio cholerae O139 emerged in 1992 as a major cause of epidemic cholera. However, the incidence of disease due to this new serogroup subsequently decreased for almost a decade. In April 2002, there...
Detection and Characterization of Human Group C Rotaviruses in Bangladesh
Rahman, Mustafizur, Banik, Sukalyani, Faruque, Abu S. G., Taniguchi, Koki, Sack, David A., Van Ranst, Marc, ...
Group C rotaviruses were detected by reverse transcription-PCR in 14 (2.3%) of 611 group A rotavirus-negative stool specimens from the patients admitted to the International Centre for Diarrhoeal...
Blood Group, Immunity, and Risk of Infection with Vibrio cholerae in an Area of Endemicity
Harris, Jason B., Khan, Ashraful I., LaRocque, Regina C., Dorer, David J., Chowdhury, Fahima, Faruque, Abu S. G., ...
Individuals with blood group O are more susceptible than other individuals to severe cholera, although the mechanism underlying this association is unknown. To assess the respective roles of both...
McKenzie, Robin, Bourgeois, A. Louis, Engstrom, Fayette, Hall, Eric, Chang, H. Sunny, Gomes, Joseph G., ...
A vaccine against enterotoxigenic Escherichia coli (ETEC) is needed to prevent diarrheal illness among children in developing countries and at-risk travelers. Two live attenuated ETEC strains, PTL002...
Alam, Munirul, Sadique, Abdus, Bhuiyan, Nurul A., Nair, G. Balakrish, Siddique, A. K., ...
It has long been assumed that prolonged holding of environmental samples at the ambient air temperature prior to bacteriological analysis is detrimental to isolation and detection of Vibrio cholerae,...
Toxigenic Vibrio cholerae in the Aquatic Environment of Mathbaria, Bangladesh
Alam, Munirul, Sultana, Marzia, Nair, G. Balakrish, Sack, R. Bradley, Sack, David A., Siddique, A. K., ...
Toxigenic Vibrio cholerae, rarely isolated from the aquatic environment between cholera epidemics, can be detected in what is now understood to be a dormant stage, i.e., viable but nonculturable when...
Faruque, Shah M., Biswas, Kuntal, Udden, S. M. Nashir, Ahmad, Qazi Shafi, Sack, David A., Nair, G. Balakrish, ...
The factors that enhance the waterborne spread of bacterial epidemics and sustain the epidemic strain in nature are unclear. Although the epidemic diarrheal disease cholera is known to be transmitted...
Raqib, Rubhana, Sarker, Protim, Bergman, Peter, Ara, Gul, Lindh, Monica, Sack, David A., ...
Shigella is a major cause of morbidity, mortality, and growth retardation for children in developing countries. Emergence of antibiotic resistance among Shigellae demands the development of effective...
Alam, Munirul, Hasan, Nur A., Sadique, Abdus, Bhuiyan, N. A., Ahmed, Kabir U., Nusrin, Suraia, ...
Since Vibrio cholerae O139 first appeared in 1992, both O1 El Tor and O139 have been recognized as the epidemic serogroups, although their geographic distribution, endemicity, and reservoir are not...
Cholera Due to Altered El Tor Strains of Vibrio cholerae O1 in Bangladesh▿
Nair, G. Balakrish, Qadri, Firdausi, Holmgren, Jan, Svennerholm, Ann-Mari, Safa, Ashrafus, Bhuiyan, Nurul A., ...
We determined the types of cholera toxin (CT) produced by a collection of 185 Vibrio cholerae O1 strains isolated in Bangladesh over the past 45 years. All of the El Tor strains of V. cholerae O1...
Global Dissemination of Vibrio parahaemolyticus Serotype O3:K6 and Its Serovariants
Nair, G. Balakrish, Ramamurthy, Thandavarayan, Bhattacharya, Sujit K., Dutta, Basabjit, Takeda, Yoshifumi, Sack, David A.
Vibrio parahaemolyticus is recognized as a cause of food-borne gastroenteritis, particularly in the Far East, where raw seafood consumption is high. An unusual increase in admissions of V....
Alam, Munirul, Sultana, Marzia, Nair, G. Balakrish, Siddique, A. K., Hasan, Nur A., Sack, R. Bradley, ...
Vibrio cholerae persists in aquatic environments predominantly in a nonculturable state. In this study coccoid, nonculturable V. cholerae O1 in biofilms maintained for 495 days in Mathbaria,...
Talukder, Kaisar A., Aslam, Mohammad, Islam, Zhahirul, Azmi, Ishrat J., Dutta, Dilip K., Hossain, Sabir, ...
From 300 stool samples, 58 Campylobacter strains were isolated by standard microbiological and biochemical methods. Of these, 40 strains were identified as Campylobacter jejuni and 5 as Campylobacter...
Enterotoxigenic Escherichia coli diarrhoea: acquired immunity and transmission in an endemic area
Black, Robert E., Merson, Michael H., Rowe, Bernard, Taylor, Philip R., Gross, Roger J., ...
Enterotoxigenic Escherichia coli (ETEC) are an important cause of diarrhoea in developing countries. Studies were made, in an endemic area of Bangladesh, of household contacts of patients with...
Improving international research contracting
Sack, David A, Brooks, Vanessa, Behan, Michael, Cravioto, Alejandro, Kennedy, Andrew, IJsselmuiden, Carel, ...
Prevalence of G2P[4] and G12P[6] Rotavirus, Bangladesh
Rahman, Mustafizur, Sultana, Rasheda, Ahmed, Giasuddin, Nahar, Sharifun, Hassan, Zahid M., Saiada, Farjana, ...
Rotavirus strains not covered by licensed vaccines are increasing.