David A. Widdick

A Facile Reporter System for the Experimental Identification of Twin-Arginine Translocation (Tat) Signal Peptides from All Kingdoms of Life (2008)

Widdick, David A., Eijlander, Robyn T., Dijl, Jan Maarten Van, Kuipers, Oscar P., Palmer, Tracy

We have developed a reporter protein system for the experimental verification of twin-arginine signal peptides. This reporter system is based on the Streptomyces coelicolor agarase protein, which is...

Formation of functional Tat translocases from heterologous components (2006)

Hicks, Matthew G, Guymer, David, Buchanan, Grant, Widdick, David A, Caldelari, Isabelle, Berks, Ben C, ...

Abstract Background The Tat pathway transports folded proteins across the cytoplasmic membrane of bacteria and the thylakoid membrane of plants. In Eschericha coli , Tat transport requires the...

Specificity Determinants of Proteolytic Processing of Aspergillus PacC Transcription Factor Are Remote from the Processing Site, and Processing Occurs in Yeast If pH Signalling Is Bypassed

Mingot, José-Manuel, Tilburn, Joan, Diez, Eliecer, Bignell, Elaine, Orejas, Margarita, Widdick, David A., ...

The Aspergillus nidulans transcription factor PacC, which mediates pH regulation, is proteolytically processed to a functional form in response to ambient alkaline pH. The full-length PacC form is...

Formation of functional Tat translocases from heterologous components

Hicks, Matthew G, Guymer, David, Buchanan, Grant, Widdick, David A, Caldelari, Isabelle, Berks, Ben C, ...

Specificity Determinants of Proteolytic Processing of Aspergillus PacC Transcription Factor Are Remote from the Processing Site, and Processing Occurs in Yeast If pH Signalling Is Bypassed

Mingot, José-Manuel, Tilburn, Joan, Diez, Eliecer, Bignell, Elaine, Orejas, Margarita, Widdick, David A., ...

The Aspergillus nidulans transcription factor PacC, which mediates pH regulation, is proteolytically processed to a functional form in response to ambient alkaline pH. The full-length PacC form is...

Formation of functional Tat translocases from heterologous components

Hicks, Matthew G, Guymer, David, Buchanan, Grant, Widdick, David A, Caldelari, Isabelle, Berks, Ben C, ...

The twin-arginine translocation pathway is a major route of protein export in Streptomyces coelicolor

Widdick, David A., Dilks, Kieran, Chandra, Govind, Bottrill, Andrew, Naldrett, Mike, Pohlschröder, Mechthild, ...

The twin-arginine translocation (Tat) pathway is a protein transport system for the export of folded proteins. Substrate proteins are targeted to the Tat translocase by N-terminal signal peptides...