David C. Tscharke

The Vaccinia Virus Superoxide Dismutase-Like Protein (A45R) Is a Virion Component That Is Nonessential for Virus Replication

Almazán, Fernando, Tscharke, David C., Smith, Geoffrey L.

A characterization of the A45R gene from vaccinia virus (VV) strain Western Reserve is presented. The open reading frame is predicted to encode a 125-amino-acid protein (Mr, of 13,600) with 39% amino...

HLA class I-restricted responses to vaccinia recognize a broad array of proteins mainly involved in virulence and viral gene regulation

Oseroff, Carla, Kos, Ferdynand, Bui, Huynh-Hoa, Peters, Bjoern, Pasquetto, Valerie, Glenn, Jean, ...

We have analyzed by ex vivo ELISPOT the anti-vaccinia cytotoxic T lymphocyte responses of peripheral blood mononuclear cells from humans vaccinated with Dryvax vaccine. More than 6,000 peptides from...

Poxvirus CD8+ T-Cell Determinants and Cross-Reactivity in BALB/c Mice

Tscharke, David C., Woo, Wai-Ping, Sakala, Isaac G., Sidney, John, Sette, Alessandro, Moss, Denis J., ...

Mouse models of orthopoxvirus disease provide great promise for probing basic questions regarding host responses to this group of pathogens, which includes the causative agents of monkeypox and...

The Vaccinia Virus Superoxide Dismutase-Like Protein (A45R) Is a Virion Component That Is Nonessential for Virus Replication

Almazán, Fernando, Tscharke, David C., Smith, Geoffrey L.

A characterization of the A45R gene from vaccinia virus (VV) strain Western Reserve is presented. The open reading frame is predicted to encode a 125-amino-acid protein (Mr, of 13,600) with 39% amino...

HLA class I-restricted responses to vaccinia recognize a broad array of proteins mainly involved in virulence and viral gene regulation

Oseroff, Carla, Kos, Ferdynand, Bui, Huynh-Hoa, Peters, Bjoern, Pasquetto, Valerie, Glenn, Jean, ...

We have analyzed by ex vivo ELISPOT the anti-vaccinia cytotoxic T lymphocyte responses of peripheral blood mononuclear cells from humans vaccinated with Dryvax vaccine. More than 6,000 peptides from...

Poxvirus CD8+ T-Cell Determinants and Cross-Reactivity in BALB/c Mice

Tscharke, David C., Woo, Wai-Ping, Sakala, Isaac G., Sidney, John, Sette, Alessandro, Moss, Denis J., ...

Mouse models of orthopoxvirus disease provide great promise for probing basic questions regarding host responses to this group of pathogens, which includes the causative agents of monkeypox and...

Identification of poxvirus CD8+ T cell determinants to enable rational design and characterization of smallpox vaccines

Tscharke, David C., Karupiah, Gunasegaran, Zhou, Jie, Palmore, Tara, Irvine, Kari R., Haeryfar, S.M. Mansour, ...

The large size of poxvirus genomes has stymied attempts to identify determinants recognized by CD8+ T cells and greatly impeded development of mouse smallpox vaccination models. Here, we use a...

Kinetic analysis of a complete poxvirus transcriptome reveals an immediate-early class of genes

Assarsson, Erika, Greenbaum, Jason A., Sundström, Magnus, Schaffer, Lana, Hammond, Jennifer A., Pasquetto, Valerie, ...

Vaccinia virus is the prototypic orthopoxvirus and was the vaccine used to eradicate smallpox, yet the expression profiles of many of its genes remain unknown. Using a genome tiling array approach,...

Regulation of protein translation through mRNA structure influences MHC class I loading and T cell recognition

Tellam, Judy, Smith, Corey, Rist, Michael, Webb, Natasha, Cooper, Leanne, Vuocolo, Tony, ...

Many viruses avoid immune surveillance during latent infection through reduction in the synthesis of virally encoded proteins. Although antigen presentation critically depends on the level of viral...