Scientific Commons: David Cowburn

The BH3 motif of the pro-survival family of proteins, BCL, is also present in pro-apoptotic proteins like BID and BAX. Homo and hetero-oligomerization interactions of the BH3 motif are generally...

NMR solution structure and backbone dynamics of domain III of the E protein of tick-borne Langat flavivirus suggests a potential site for molecular recognition (2006)

Mukherjee, Munia, Dutta, Kaushik, White, Mark A., Cowburn, David, Fox, Robert O.

Flaviviruses cause many human diseases, including dengue fever, yellow fever, West Nile viral encephalitis, and hemorrhagic fevers, and are transmitted to their vertebrate hosts by infected...

The structure of the IgE Cɛ2 domain and its role in stabilizing the complex with its high-affinity receptor FcɛRIα (2001)

James M. McDonnell, Rosaleen Calvert, Rebecca L. Beavil, Andrew J. Beavil, Alistair J. Henry, Brian J. Sutton, ...

The Virtual NMR Spectrometer: A Computer Program for Efficient Simulation of NMR Experiments Involving Pulsed Field Gradients (2000)

Peter Nicholas, David Fushman, Vladislav Ruchinsky, David Cowburn

This paper presents a software program, the Virtual NMR Spectrometer, for computer simulation of multichannel, multidimensional NMR experiments on user-defined spin systems. The program is capable of...

Rational Design of Regulators of Programmed Cell Death in Human Breast Cancer (1998)

Cowburn, David

The purpose of this research is to develop a better understanding of the intricate pathways of cell death and their contributions to breast cancers, with the goal of designing potential therapeutic...

Secondary structure of Src homology 2 domain of c-Abl by heteronouclear NMR spectroscopy in solution (1992)

Overduin, Michael, Mayer, Bruce, Rios, Carlos B., Baltimore, David, Cowburn, David

The Src homology 2 (SH2) domain is a recognition motif thought to mediate the association of the cytoplasmic proteins involved in signal transduction by binding to phosphotyrosyl-containing sequences...

Chemical ligation of folded recombinant proteins: Segmental isotopic labeling of domains for NMR studies

Xu, Rong, Ayers, Brenda, Cowburn, David, Muir, Tom W.

A convenient in vitro chemical ligation strategy has been developed that allows folded recombinant proteins to be joined together. This strategy permits segmental, selective isotopic labeling of the...

Autoregulation of a bacterial σ factor explored by using segmental isotopic labeling and NMR

Camarero, Julio A., Shekhtman, Alexander, Campbell, Elizabeth A., Chlenov, Mark, Gruber, Tanja M., Bryant, Donald A., ...

Bacterial σ factors combine with the catalytic core RNA polymerase to direct the process of transcription initiation through sequence-specific interactions with the −10 and −35 elements of...

Semisynthesis of a segmental isotopically labeled protein splicing precursor: NMR evidence for an unusual peptide bond at the N-extein–intein junction

Romanelli, Alessandra, Shekhtman, Alexander, Cowburn, David, Muir, Tom W.

Protein splicing is a posttranslational autocatalytic process in which an intervening sequence, termed an intein, is removed from a host protein, the extein. Although we have a reasonable picture of...

The Affinity-Labeling of Partially Purified Acetylcholine Receptor from Electric Tissue of Electrophorus

Karlin, Arthur, Cowburn, David

The receptor for acetylcholine was partially purified by affinity chromatography of an extract in Triton X-100 of membrane fragments from electric tissue. The receptor was assayed, after its...