Yang-xin Fu, Hector Molina, Mitsuru Matsumoto, Guangming Huang, Jingjuan Min, David D. Chaplin
mice show altered splenic microarchitecture. This includes loss of normal B cell–T cell compartmentalization, of follicular dendritic cell (FDC) clusters, and of ability to form germinal centers...
Sandip A. Godambe, David D. Chaplin, Tsetska Takova, Lory M. Read, J. Bellone
Regulatory elements important for transcription of the murine interleukin-1 � (IL-1�) gene lie within a DNase I-hypersensitive region located>2,000 bp upstream from the transcription start...
Krishnan, B. Rajendra, Jamry, Isabela, Berg, Douglas E., Berg, Claire M., Chaplin, David D.
We are applying a transposon-based approach for detecting and mapping features of special interest to construct ‘feature maps’ of currently uncharacterized portions of the human leukocyte antigen...
Graham, Madge Y., Otani, Tetsuo, Boime, Irving, Olson, Maynard V., Carle, Georges F., Chaplin, David D.
A cosmid clone containing the entire hCGβ gene cluster has been isolated. The restriction map of this clone has been determined by an indirect-end-label FIGE (field inversion gel electrophoresis)...
Fu, Yang-Xin, Huang, Guangming, Matsumoto, Mitsuru, Molina, Hector, Chaplin, David D.
Lymphotoxin-α-deficient (LT-α−/−) mice manifest congenital absence of lymph nodes (LNs) and Peyer’s patches and disturbed spleen follicle structure. The splenic white pulp areas show loss of...
Abrogation of the alternative complement pathway by targeted deletion of murine factor B
Matsumoto, Mitsuru, Fukuda, Wataru, Circolo, Antonella, Goellner, Joseph, Strauss-Schoenberger, Jena, Wang, Xuefeng, ...
To investigate the role of complement protein factor B (Bf) and alternative pathway activity in vivo, and to test the hypothesized potential genetic lethal effect of Bf deficiency, the murine Bf gene...
Requirement for membrane lymphotoxin in natural killer cell development
Iizuka, Koho, Chaplin, David D., Wang, Yang, Wu, Qiang, Pegg, Lyle E., Yokoyama, Wayne M., ...
Development of natural killer (NK) cells is thought to depend on interactions between NK progenitors and the bone marrow (BM) microenvironment; however, little is known about the molecular signals...
A role for IL-1α in inducing pathologic inflammation during bacterial infection
Dube, Peter H., Revell, Paula A., Chaplin, David D., Lorenz, Robin G., Miller, Virginia L.
Infection with pathogenic microbes often results in a significant inflammatory response. A cascade of proinflammatory cytokines including tumor necrosis factor α (TNF-α) and IL-1 initiates this...
Cooperation between Th1 and Th2 cells in a murine model of eosinophilic airway inflammation
Randolph, David A., Stephens, Robin, Carruthers, Cynthia J.L., Chaplin, David D.
We have studied the actions of helper T lymphocyte-1 and -2 (Th1 and Th2) cells in an acute model of eosinophilic airway inflammation by infusing chicken ovalbumin-specific (OVA-specific) Th1 cells,...
Chaplin, David D., Wedner, H. James, Parker, Charles W.
Phosphorylation of endogenous proteins in subcellular fractions of human peripheral-blood lymphocytes was studied by one- and two-dimensional polyacrylamide-gel electrophoresis. Studies using...
Fu, Yang-Xin, Huang, Guangming, Matsumoto, Mitsuru, Molina, Hector, Chaplin, David D.
Lymphotoxin-α-deficient (LT-α−/−) mice manifest congenital absence of lymph nodes (LNs) and Peyer’s patches and disturbed spleen follicle structure. The splenic white pulp areas show loss of...
Abrogation of the alternative complement pathway by targeted deletion of murine factor B
Matsumoto, Mitsuru, Fukuda, Wataru, Circolo, Antonella, Goellner, Joseph, Strauss-Schoenberger, Jena, Wang, Xuefeng, ...
To investigate the role of complement protein factor B (Bf) and alternative pathway activity in vivo, and to test the hypothesized potential genetic lethal effect of Bf deficiency, the murine Bf gene...
Requirement for membrane lymphotoxin in natural killer cell development
Iizuka, Koho, Chaplin, David D., Wang, Yang, Wu, Qiang, Pegg, Lyle E., Yokoyama, Wayne M., ...
Development of natural killer (NK) cells is thought to depend on interactions between NK progenitors and the bone marrow (BM) microenvironment; however, little is known about the molecular signals...
A role for IL-1α in inducing pathologic inflammation during bacterial infection
Dube, Peter H., Revell, Paula A., Chaplin, David D., Lorenz, Robin G., Miller, Virginia L.
Infection with pathogenic microbes often results in a significant inflammatory response. A cascade of proinflammatory cytokines including tumor necrosis factor α (TNF-α) and IL-1 initiates this...
Cooperation between Th1 and Th2 cells in a murine model of eosinophilic airway inflammation
Randolph, David A., Stephens, Robin, Carruthers, Cynthia J.L., Chaplin, David D.
We have studied the actions of helper T lymphocyte-1 and -2 (Th1 and Th2) cells in an acute model of eosinophilic airway inflammation by infusing chicken ovalbumin-specific (OVA-specific) Th1 cells,...
Chaplin, David D., Wedner, H. James, Parker, Charles W.
Phosphorylation of endogenous proteins in subcellular fractions of human peripheral-blood lymphocytes was studied by one- and two-dimensional polyacrylamide-gel electrophoresis. Studies using...
Jung, Yong Woo, Schoeb, Trenton R., Weaver, Casey T., Chaplin, David D.
T helper (Th) cells play major roles in orchestrating asthmatic airway inflammation, but the molecular mechanisms controlling Th-cell recruitment to the airways remain incompletely defined. Innate...
Essential Role of Lymph Nodes in Contact Hypersensitivity Revealed in Lymphotoxin-α–Deficient Mice
Rennert, Paul D., Hochman, Paula S., Flavell, Richard A., Chaplin, David D., Jayaraman, Sundararajan, Browning, Jeffrey L., ...
Lymph nodes (LNs) are important sentinal organs, populated by circulating lymphocytes and antigen-bearing cells exiting the tissue beds. Although cellular and humoral immune responses are induced in...
Fu, Yang-Xin, Molina, Hector, Matsumoto, Mitsuru, Huang, Guangming, Min, Jingjuan, Chaplin, David D.
LTα-deficient (LTα−/−) mice show altered splenic microarchitecture. This includes loss of normal B cell–T cell compartmentalization, of follicular dendritic cell (FDC) clusters, and of...
Matsumoto, Mitsuru, Fu, Yang-Xin, Molina, Hector, Huang, Guangming, Kim, Jinho, Thomas, Dori A., ...
In mice deficient in either lymphotoxin α (LT-α) or type I tumor necrosis factor receptor (TNFR-I), organized clusters of follicular dendritic cells (FDC) and germinal centers (GC) are absent from...
Fu, Yang-Xin, Huang, Guangming, Wang, Yang, Chaplin, David D.
Lymphotoxin (LT)α is expressed by activated T cells, especially CD4+ T helper type 1 cells, and by activated B and natural killer cells, but the functions of this molecule in vivo are incompletely...
THE EFFECTS OF THROMBIN ON PHYTOHEMAGGLUTININ RECEPTOR SITES IN HUMAN PLATELETS
Feagler, John R., Tillack, Thomas W., Chaplin, David D., Majerus, Philip W.
We have previously demonstrated that lentil phytohemagglutinin (lentil-PHA) binds to human platelet membranes without causing either aggregation or the release reaction. When platelets are treated...
Matsumoto, Mitsuru, Zhou, Yiqing, Matsuo, Shinji, Nakanishi, Hideki, Hirose, Kenji, Oura, Hajimu, ...
Controlled proteolytic degradation of specialized junctional structures, corneodesmosomes, by epidermal proteases is an essential process for physiological desquamation of the skin. Corneodesmosin...