David E. Ott

CD45 immunoaffinity depletion of vesicles from Jurkat T cells demonstrates that exosomes contain CD45: no evidence for a distinct exosome/HIV-1 budding pathway (2008)

Coren, Lori V, Shatzer, Teresa, Ott, David E

Abstract The presence of relatively high levels of cellular protein contamination in density-purified virion preparations is a confounding factor in biochemical analyses of HIV and SIV produced from...

An Open Architecture for Transport-level Protocol Coordination in Distributed Multimedia Applications (2008)

David E. Ott, Ketan Mayer-patel

We consider the problem of flow coordination in distributed multimedia applications. Most transport-level protocols are designed to operate independently and lack mechanisms for sharing information...

Transport-level Protocol Coordination in Distributed Multimedia Applications (2007)

David E. Ott, Ketan Mayer-Patel

this dissertation, we argue that cluster-to-cluster applications like O#ce of the Future could significantly benefit from network mechanisms that facilitate transport-level protocol coordination of...

Elimination of Retroviral Infectivity by N-Ethylmaleimide with Preservation of Functional Envelope Glycoproteins (2004)

David R. Morcock, James A. Thomas, Tracy D. Gagliardi, Robert J. Gorelick, J. David Roser, Elena N. Chertova, ...

The zinc finger motifs in retroviral nucleocapsid (NC) proteins are essential for viral replication. Disruption of these Cys-X 2-Cys-X 4-His-X 4-Cys zinc-binding structures eliminates infectivity. To...

Dynamic Fluorescent Imaging of Human Immunodeficiency Virus Type 1 Gag in Live Cells by Biarsenical Labeling (2004)

Lynnie Rudner, Sascha Nydegger, Lori V. Coren, Kunio Nagashima, Markus Thali, David E. Ott

Human immunodeficiency virus type 1 (HIV-1) Gag is the primary structural protein of the virus and is sufficient for particle formation. We utilized the recently developed biarsenical-labeling method...

Aggregate Congestion Control for Distributed Multimedia Applications (2004)

David E. Ott, Travis Sparks, Ketan Mayer-Patel

We consider the problem of applying aggregate congestion control to a class of distributed multimedia applications known as Cluster-to-Cluster (C-to-C) applications. Flows in such an application...

Aggregate Congestion Control for Distributed Multimedia Applications (2004)

David E. Ott, Travis Sparks, Ketan Mayer-Patel

We consider the problem of applying aggregate congestion control to a class of distributed multimedia applications known as Cluster-to-Cluster (C-to-C) applications. Flows in such an application...

Transport-level protocol coordination in cluster-to-cluster applications (2002)

David E. Ott, Ketan Mayer-patel

Future Internet applications will increasingly use multiple communications and computing devices in a distributed fashion. In this paper, we identify an emerging and important application class...

A Mechanism for TCP-Friendly Transport-level Protocol Coordination (2002)

David E. Ott, Ketan Mayer-patel

In this paper, we identify an emerging and important application class comprised of a set of processes on a cluster of devices communicating to a remote set of processes on another cluster of devices...

Transport-level Protocol Coordination in Cluster-to-Cluster Applications (2001)

David E. Ott, Ketan Mayer-patel

Future Internet applications will increasingly use multiple communications and computing devices in a distributed fashion. In this paper, we identify an emerging and important application class...

Molecular requirements for immunoglobulin heavy chain constant region gene switch recombination revealed with switch substrate retroviruses (1989)

Ott, David E., Marcu, Kenneth B., Tucker, Phillip W

We have employed a retroviral vector, ZN(Sμ,/Sγ2b)tk1, as a means of introducing immunoglobulin heavy chain (IgH) swltch (S) region sequences into B cell lines to directly measure their switch...

Proteasome inhibition interferes with Gag polyprotein processing, release, and maturation of HIV-1 and HIV-2

Schubert, Ulrich, Ott, David E., Chertova, Elena N., Welker, Reinhold, Tessmer, Uwe, Princiotta, Michael F., ...

Retrovirus assembly and maturation involve folding and transport of viral proteins to the virus assembly site followed by subsequent proteolytic cleavage of the Gag polyprotein within the nascent...

Mutational Analysis of the Hydrophobic Tail of the Human Immunodeficiency Virus Type 1 p6Gag Protein Produces a Mutant That Fails To Package Its Envelope Protein

Ott, David E., Chertova, Elena N., Busch, Laura K., Coren, Lori V., Gagliardi, Tracy D., Johnson, Donald G.

The p6Gag protein of human immunodeficiency virus type 1 (HIV-1) is produced as the carboxyl-terminal sequence within the Gag polyprotein. The amino acid composition of this protein is high in...

Ubiquitin Is Covalently Attached to the p6Gag Proteins of Human Immunodeficiency Virus Type 1 and Simian Immunodeficiency Virus and to the p12Gag Protein of Moloney Murine Leukemia Virus

Ott, David E., Coren, Lori V., Copeland, Terry D., Kane, Bradley P., Johnson, Donald G., Sowder, Raymond C., ...

Host proteins are incorporated into retroviral virions during assembly and budding. We have examined three retroviruses, human immunodeficiency virus type 1 (HIV-1), simian immunodeficiency virus...

Efficient Incorporation of HLA Class II onto Human Immunodeficiency Virus Type 1 Requires Envelope Glycoprotein Packaging

Poon, Dexter T. K., Coren, Lori V., Ott, David E.

HLA class II DR is one of the most abundant cell surface proteins incorporated onto human immunodeficiency virus type 1 (HIV-1) during budding. The mechanism for HLA class II protein incorporation is...

Equine Infectious Anemia Virus and the Ubiquitin-Proteasome System

Ott, David E., Coren, Lori V., Sowder, Raymond C., Adams, Julian, Nagashima, Kunio, Schubert, Ulrich

Some retroviruses contain monoubiquitinated Gag and do not bud efficiently from cells treated with proteasome inhibitors, suggesting an interaction between the ubiquitin-proteasome system and...

Retroviruses Have Differing Requirements for Proteasome Function in the Budding Process

Ott, David E., Coren, Lori V., Sowder II, Raymond C., Adams, Julian, Schubert, Ulrich

Proteasome inhibitors reduce the budding of human immunodeficiency virus types 1 (HIV-1) and 2, simian immunodeficiency virus, and Rous sarcoma virus. To investigate this effect further, we examined...

Elimination of Protease Activity Restores Efficient Virion Production to a Human Immunodeficiency Virus Type 1 Nucleocapsid Deletion Mutant

Ott, David E., Coren, Lori V., Chertova, Elena N., Gagliardi, Tracy D., Nagashima, Kunio, Sowder II, Raymond C., ...

The nucleocapsid (NC) region of human immunodeficiency virus type 1 (HIV-1) Gag is required for specific genomic RNA packaging. To determine if NC is absolutely required for virion formation, we...

Quantitation of HLA Class II Protein Incorporated into Human Immunodeficiency Type 1 Virions Purified by Anti-CD45 Immunoaffinity Depletion of Microvesicles

Trubey, Charles M., Chertova, Elena, Coren, Lori V., Hilburn, Joanne M., Hixson, Catherine V., Nagashima, Kunio, ...

Among the many host cell-derived proteins found in human immunodeficiency virus type 1 (HIV-1), HLA class II (HLA-II) appears to be selectively incorporated onto virions and may contribute to...

Elimination of Retroviral Infectivity by N-Ethylmaleimide with Preservation of Functional Envelope Glycoproteins

Morcock, David R., Thomas, James A., Gagliardi, Tracy D., Gorelick, Robert J., Roser, J. David, Chertova, Elena N., ...

The zinc finger motifs in retroviral nucleocapsid (NC) proteins are essential for viral replication. Disruption of these Cys-X2-Cys-X4-His-X4-Cys zinc-binding structures eliminates infectivity. To...

Human Immunodeficiency Virus Type 1-Induced Macrophage Gene Expression Includes the p21 Gene, a Target for Viral Regulation

Vázquez, Nancy, Greenwell-Wild, Teresa, Marinos, Nancy J., Swaim, William D., Nares, Salvador, Ott, David E., ...

In contrast to CD4+ T cells, human immunodeficiency virus type 1 (HIV-1)-infected macrophages typically resist cell death, support viral replication, and consequently, may facilitate HIV-1...

Dynamic Fluorescent Imaging of Human Immunodeficiency Virus Type 1 Gag in Live Cells by Biarsenical Labeling

Rudner, Lynnie, Nydegger, Sascha, Coren, Lori V., Nagashima, Kunio, Thali, Markus, Ott, David E.

Human immunodeficiency virus type 1 (HIV-1) Gag is the primary structural protein of the virus and is sufficient for particle formation. We utilized the recently developed biarsenical-labeling method...

Redundant Roles for Nucleocapsid and Matrix RNA-Binding Sequences in Human Immunodeficiency Virus Type 1 Assembly

Ott, David E., Coren, Lori V., Gagliardi, Tracy D.

RNA appears to be required for the assembly of retroviruses. This is likely due to binding of RNA by multiple Gags, which in turn organizes and stabilizes the Gag-Gag interactions that form the...

Heterologous Late-Domain Sequences Have Various Abilities To Promote Budding of Human Immunodeficiency Virus Type 1

Ott, David E., Coren, Lori V., Gagliardi, Tracy D., Nagashima, Kunio

Retroviral late (L) domains present within Gag act in conjunction with cellular proteins to efficiently release virions from the surface of the cell. Three different critical core sequences have been...

Proteasome inhibition interferes with Gag polyprotein processing, release, and maturation of HIV-1 and HIV-2

Schubert, Ulrich, Ott, David E., Chertova, Elena N., Welker, Reinhold, Tessmer, Uwe, Princiotta, Michael F., ...

Retrovirus assembly and maturation involve folding and transport of viral proteins to the virus assembly site followed by subsequent proteolytic cleavage of the Gag polyprotein within the nascent...

Mutational Analysis of the Hydrophobic Tail of the Human Immunodeficiency Virus Type 1 p6Gag Protein Produces a Mutant That Fails To Package Its Envelope Protein

Ott, David E., Chertova, Elena N., Busch, Laura K., Coren, Lori V., Gagliardi, Tracy D., Johnson, Donald G.

The p6Gag protein of human immunodeficiency virus type 1 (HIV-1) is produced as the carboxyl-terminal sequence within the Gag polyprotein. The amino acid composition of this protein is high in...

Ubiquitin Is Covalently Attached to the p6Gag Proteins of Human Immunodeficiency Virus Type 1 and Simian Immunodeficiency Virus and to the p12Gag Protein of Moloney Murine Leukemia Virus

Ott, David E., Coren, Lori V., Copeland, Terry D., Kane, Bradley P., Johnson, Donald G., Sowder, Raymond C., ...

Host proteins are incorporated into retroviral virions during assembly and budding. We have examined three retroviruses, human immunodeficiency virus type 1 (HIV-1), simian immunodeficiency virus...

Efficient Incorporation of HLA Class II onto Human Immunodeficiency Virus Type 1 Requires Envelope Glycoprotein Packaging

Poon, Dexter T. K., Coren, Lori V., Ott, David E.

HLA class II DR is one of the most abundant cell surface proteins incorporated onto human immunodeficiency virus type 1 (HIV-1) during budding. The mechanism for HLA class II protein incorporation is...

Equine Infectious Anemia Virus and the Ubiquitin-Proteasome System

Ott, David E., Coren, Lori V., Sowder, Raymond C., Adams, Julian, Nagashima, Kunio, Schubert, Ulrich

Some retroviruses contain monoubiquitinated Gag and do not bud efficiently from cells treated with proteasome inhibitors, suggesting an interaction between the ubiquitin-proteasome system and...

Retroviruses Have Differing Requirements for Proteasome Function in the Budding Process

Ott, David E., Coren, Lori V., Sowder II, Raymond C., Adams, Julian, Schubert, Ulrich

Proteasome inhibitors reduce the budding of human immunodeficiency virus types 1 (HIV-1) and 2, simian immunodeficiency virus, and Rous sarcoma virus. To investigate this effect further, we examined...

Elimination of Protease Activity Restores Efficient Virion Production to a Human Immunodeficiency Virus Type 1 Nucleocapsid Deletion Mutant

Ott, David E., Coren, Lori V., Chertova, Elena N., Gagliardi, Tracy D., Nagashima, Kunio, Sowder II, Raymond C., ...

The nucleocapsid (NC) region of human immunodeficiency virus type 1 (HIV-1) Gag is required for specific genomic RNA packaging. To determine if NC is absolutely required for virion formation, we...

Quantitation of HLA Class II Protein Incorporated into Human Immunodeficiency Type 1 Virions Purified by Anti-CD45 Immunoaffinity Depletion of Microvesicles

Trubey, Charles M., Chertova, Elena, Coren, Lori V., Hilburn, Joanne M., Hixson, Catherine V., Nagashima, Kunio, ...

Among the many host cell-derived proteins found in human immunodeficiency virus type 1 (HIV-1), HLA class II (HLA-II) appears to be selectively incorporated onto virions and may contribute to...

Elimination of Retroviral Infectivity by N-Ethylmaleimide with Preservation of Functional Envelope Glycoproteins

Morcock, David R., Thomas, James A., Gagliardi, Tracy D., Gorelick, Robert J., Roser, J. David, Chertova, Elena N., ...

The zinc finger motifs in retroviral nucleocapsid (NC) proteins are essential for viral replication. Disruption of these Cys-X2-Cys-X4-His-X4-Cys zinc-binding structures eliminates infectivity. To...

Human Immunodeficiency Virus Type 1-Induced Macrophage Gene Expression Includes the p21 Gene, a Target for Viral Regulation

Vázquez, Nancy, Greenwell-Wild, Teresa, Marinos, Nancy J., Swaim, William D., Nares, Salvador, Ott, David E., ...

In contrast to CD4+ T cells, human immunodeficiency virus type 1 (HIV-1)-infected macrophages typically resist cell death, support viral replication, and consequently, may facilitate HIV-1...

Dynamic Fluorescent Imaging of Human Immunodeficiency Virus Type 1 Gag in Live Cells by Biarsenical Labeling

Rudner, Lynnie, Nydegger, Sascha, Coren, Lori V., Nagashima, Kunio, Thali, Markus, Ott, David E.

Human immunodeficiency virus type 1 (HIV-1) Gag is the primary structural protein of the virus and is sufficient for particle formation. We utilized the recently developed biarsenical-labeling method...

Heterologous Late-Domain Sequences Have Various Abilities To Promote Budding of Human Immunodeficiency Virus Type 1

Ott, David E., Coren, Lori V., Gagliardi, Tracy D., Nagashima, Kunio

Retroviral late (L) domains present within Gag act in conjunction with cellular proteins to efficiently release virions from the surface of the cell. Three different critical core sequences have been...

Redundant Roles for Nucleocapsid and Matrix RNA-Binding Sequences in Human Immunodeficiency Virus Type 1 Assembly

Ott, David E., Coren, Lori V., Gagliardi, Tracy D.

RNA appears to be required for the assembly of retroviruses. This is likely due to binding of RNA by multiple Gags, which in turn organizes and stabilizes the Gag-Gag interactions that form the...

Proteomic and Biochemical Analysis of Purified Human Immunodeficiency Virus Type 1 Produced from Infected Monocyte-Derived Macrophages

Chertova, Elena, Chertov, Oleg, Coren, Lori V., Roser, James D., Trubey, Charles M., Bess, Julian W., ...

Human immunodeficiency virus type 1 (HIV-1) infects CD4+ T lymphocytes and monocytes/macrophages, incorporating host proteins in the process of assembly and budding. Analysis of the host cell...

Physiological Levels of Virion-Associated Human Immunodeficiency Virus Type 1 Envelope Induce Coreceptor-Dependent Calcium Flux▿ †

Melar, Marta, Ott, David E., Hope, Thomas J.

Human immunodeficiency virus (HIV) entry into target cells requires the engagement of receptor and coreceptor by envelope glycoprotein (Env). Coreceptors CCR5 and CXCR4 are chemokine receptors that...

Efficiency of Human Immunodeficiency Virus Type 1 Postentry Infection Processes: Evidence against Disproportionate Numbers of Defective Virions▿

Thomas, James A., Ott, David E., Gorelick, Robert J.

The vast majority of human immunodeficiency virus type 1 particles are claimed to be noninfectious, but there is disagreement as to whether they are defective or simply lack the opportunity to...

Mutational Analysis of the C-Terminal Gag Cleavage Sites in Human Immunodeficiency Virus Type 1▿ †

Coren, Lori V., Thomas, James A., Chertova, Elena, Sowder, Raymond C., Gagliardi, Tracy D., Gorelick, Robert J., ...

Human immunodeficiency virus type 1 (HIV-1) Gag is expressed as a polyprotein that is cleaved into six proteins by the viral protease in a maturation process that begins during assembly and budding....

Real-Time Visualization of HIV-1 GAG Trafficking in Infected Macrophages

Gousset, Karine, Ablan, Sherimay D., Coren, Lori V., Ono, Akira, Soheilian, Ferri, Nagashima, Kunio, ...

HIV-1 particle production is driven by the Gag precursor protein Pr55Gag. Despite significant progress in defining both the viral and cellular determinants of HIV-1 assembly and release, the...

CD45 immunoaffinity depletion of vesicles from Jurkat T cells demonstrates that exosomes contain CD45: no evidence for a distinct exosome/HIV-1 budding pathway

Coren, Lori V, Shatzer, Teresa, Ott, David E

The presence of relatively high levels of cellular protein contamination in density-purified virion preparations is a confounding factor in biochemical analyses of HIV and SIV produced from...

Retroviruses Human Immunodeficiency Virus and Murine Leukemia Virus Are Enriched in Phosphoinositides▿ †

Chan, Robin, Uchil, Pradeep D., Jin, Jing, Shui, Guanghou, Ott, David E., Mothes, Walther, ...

Retroviruses acquire a lipid envelope during budding from the membrane of their hosts. Therefore, the composition of this envelope can provide important information about the budding process and its...