David H. Martin

Short tandem repeat sequences in the Mycoplasma genitaliumgenome and their use in a multilocus genotyping system (2008)

Ma, Liang, Taylor, Stephanie, Jensen, Jørgen S, Myers, Leann, Lillis, Rebecca, Martin, David H

Abstract Background Several methods have been reported for strain typing of Mycoplasma genitalium . The value of these methods has never been comparatively assessed. The aims of this study were: 1)...

Association of Atopobium vaginae, a recently described metronidazole resistant anaerobe, with bacterial vaginosis (2004)

Ferris, Michael J, Masztal, Alicia, Aldridge, Kenneth E, Fortenberry, J Dennis, Fidel, Paul L, Martin, David H

Abstract Background Bacterial vaginosis (BV) is a polymicrobial syndrome characterized by a change in vaginal flora away from predominantly Lactobacillus species. The cause of BV is unknown, but the...

Use of Species-Directed 16S rRNA Gene PCR Primers for Detection of Atopobium vaginae in Patients with Bacterial Vaginosis (2004)

Michael J. Ferris, Alicia Masztal, David H. Martin

Recent studies suggest that the association between a metronidazole-resistant anaerobe, Atopobium vaginae, and bacterial vaginosis (BV) warrants further investigation. In the present study, specific...

Application of Computer-assisted Interviews to Sexual Behavior Research (1999)

Kissinger, Patricia, Rice, Janet, Farley, Thomas, Trim, Shelly, Jewitt, Kayla, Margavio, Victor, ...

Collection of sensitive data with the use of video-enhanced, computer-assisted, self-administered interviews (V-CASI) has the potential to reduce interview bias and improve the validity of the study....

Double-Blind Placebo-Controlled Treatment Trial of Chlamydia trachomatis Endocervical Infections in Pregnant Women (1997)

David H. Martin, David A. Eschenbach, Mary Frances Cotch, Robert P. Nugent, A. Vijaya Rao, Mark A. Klebanoff, ...

Objective: The purpose of this study was to determine if treatment of pregnant women with Chlamydia trachomatis infection would lower the incidence of preterm delivery and/or low birth...

AN EPIDEMIOLOGIC STUDY OF VENEZUELAN EQUINE ENCEPHALOMYELITIS IN COSTA RICA, 1970 (1972)

MARTIN, DAVID H., EDDY, GERALD A., SUDIA, W. DANIEL, REEVES, WILLIAM C., NEWHOUSE, VERNON F., JOHNSON, KARL M.

Martin, D. H., G. A. Eddy, W. D. Sudia, W. C. Reeves, V. F. Newhouse and K. M. Johnson (MARU, Box 2011, Balboa Heights, Canal Zone). An epidemiologic study of Venezuelan equine encephalomyelitis in...

Multicenter Evaluation of the AMPLICOR and Automated COBAS AMPLICOR CT/NG Tests for Detection of Chlamydia trachomatis

Van Der Pol, Barbara, Quinn, Thomas C., Gaydos, Charlotte A., Crotchfelt, Kimberly, Schachter, Julius, Moncada, Jeanne, ...

The fully automated COBAS AMPLICOR CT/NG and semiautomated AMPLICOR CT/NG tests were evaluated in a multicenter trial for the ability to detect Chlamydia trachomatis infections. Test performance...

Multicenter Evaluation of AMPLICOR and Automated COBAS AMPLICOR CT/NG Tests for Neisseria gonorrhoeae

Martin, David H., Cammarata, Cathy, Van Der Pol, Barbara, Jones, Robert B., Quinn, Thomas C., Gaydos, Charlotte A., ...

The fully automated COBAS AMPLICOR CT/NG and semiautomated AMPLICOR CT/NG tests were evaluated in a multicenter trial for their ability to detect Neisseria gonorrhoeae infections. Test performance...

Evaluation of Nucleic Acid Amplification Tests as Reference Tests for Chlamydia trachomatis Infections in Asymptomatic Men

Johnson, Robert E., Green, Timothy A., Schachter, Julius, Jones, Robert B., Hook, Edward W., Black, Carolyn M., ...

Urine ligase chain reaction (LCR) and PCR tests and urethral swab culture were compared for their abilities to detect Chlamydia trachomatis infection in 3,639 asymptomatic men by using one-, two-,...

Enhancing the Specificity of the COBAS AMPLICOR CT/NG Test for Neisseria gonorrhoeae by Retesting Specimens with Equivocal Results

Van Der Pol, Barbara, Martin, David H., Schachter, Julius, Quinn, Thomas C., Gaydos, Charlotte A., Jones, Robert B., ...

The COBAS AMPLICOR CT/NG test for Neisseria gonorrhoeae cross-reacts with certain strains of nonpathogenic Neisseria species. In some strains, the target sequence is identical to that of N....

Head-to-Head Multicenter Comparison of DNA Probe and Nucleic Acid Amplification Tests for Chlamydia trachomatis Infection in Women Performed with an Improved Reference Standard

Black, Carolyn M., Marrazzo, Jeanne, Johnson, Robert E., Hook III, Edward W., Jones, Robert B., Green, Timothy A., ...

Few evaluations of tests for Chlamydia trachomatis have compared nucleic acid amplification tests (NAATs) with diagnostic tests other than those by culture. In a five-city study of 3,551 women, we...

Vaginal Swabs Are Appropriate Specimens for Diagnosis of Genital Tract Infection with Chlamydia trachomatis

Schachter, Julius, McCormack, William M., Chernesky, Max A., Martin, David H., Van Der Pol, Barbara, Rice, Peter A., ...

Because self-collected vaginal swabs (VS) are potentially very useful for screening asymptomatic women for Chlamydia trachomatis infection, a multicenter study evaluated that specimen with nucleic...

An Intravaginal Live Candida Challenge in Humans Leads to New Hypotheses for the Immunopathogenesis of Vulvovaginal Candidiasis

Fidel, Paul L., Barousse, Melissa, Espinosa, Terri, Ficarra, Mercedes, Sturtevant, Joy, Martin, David H., ...

Acute and recurrent vulvovaginal candidiasis (VVC) remains a significant problem in women of childbearing age. While clinical studies of women with recurrent VVC (RVVC) and animal models have...

Population-Based Genetic and Evolutionary Analysis of Chlamydia trachomatis Urogenital Strain Variation in the United States†

Millman, Kim, Black, Carolyn M., Johnson, Robert E., Stamm, Walter E., Jones, Robert B., Hook, Edward W., ...

Chlamydia trachomatis is a major cause of ocular and sexually transmitted diseases worldwide. While much of our knowledge about its genetic diversity comes from serotyping or ompA genotyping, no...

Field Studies of an Attenuated Venezuelan Equine Encephalomyelitis Vaccine (Strain TC-83)

Eddy, Gerald A., Martin, David H., Reeves, William C., Johnson, Karl M.

A series of field studies using strain TC-83 attenuated Venezuelan equine encephalomyelitis vaccine in horses was made to determine the rate of seroconversions, the postvaccination viremia, and the...

Use of Multiple Nucleic Acid Amplification Tests To Define the Infected-Patient “Gold Standard” in Clinical Trials of New Diagnostic Tests for Chlamydia trachomatis Infections

Martin, David H., Nsuami, Malanda, Schachter, Julius, Hook, Edward W., Ferrero, Dennis, Quinn, Thomas C., ...

Nucleic acid amplification tests (NAATs) can be used to define the infected-patient “gold standard” for the purpose of designing studies of the performance of Chlamydia trachomatis diagnostic...

Single-Nucleotide Polymorphisms in the rRNA Operon and Variable Numbers of Tandem Repeats in the Lipoprotein Gene among Mycoplasma genitalium Strains from Clinical Specimens

Ma, Liang, Martin, David H.

We characterized the single-nucleotide polymorphisms in the rRNA operon and variable numbers of tandem repeats in the lipoprotein gene MG309 among Mycoplasma genitalium strains from clinical...

Use of Species-Directed 16S rRNA Gene PCR Primers for Detection of Atopobium vaginae in Patients with Bacterial Vaginosis

Ferris, Michael J., Masztal, Alicia, Martin, David H.

Recent studies suggest that the association between a metronidazole-resistant anaerobe, Atopobium vaginae, and bacterial vaginosis (BV) warrants further investigation. In the present study, specific...

Impact of Patient Characteristics on Performance of Nucleic Acid Amplification Tests and DNA Probe for Detection of Chlamydia trachomatis in Women with Genital Infections

Marrazzo, Jeanne M., Johnson, Robert E., Green, Timothy A., Stamm, Walter E., Schachter, Julius, Bolan, Gail, ...

The performance of nucleic acid amplified tests (NAAT) for Chlamydia trachomatis at the cervix and in urine was examined in 3,551 women, and the impacts of clinical findings (age, endocervical and...

Sequence-Based Typing of Mycoplasma genitalium Reveals Sexual Transmission†

Hjorth, Sofie Vetli, Björnelius, Eva, Lidbrink, Peter, Falk, Lars, Dohn, Birthe, Berthelsen, Lene, ...

Mycoplasma genitalium causes male nonchlamydial, nongonococcal urethritis and is associated with cervicitis and pelvic inflammatory disease in women. Epidemiological studies indicate that M....

Multicenter Evaluation of the AMPLICOR and Automated COBAS AMPLICOR CT/NG Tests for Detection of Chlamydia trachomatis

Van Der Pol, Barbara, Quinn, Thomas C., Gaydos, Charlotte A., Crotchfelt, Kimberly, Schachter, Julius, Moncada, Jeanne, ...

The fully automated COBAS AMPLICOR CT/NG and semiautomated AMPLICOR CT/NG tests were evaluated in a multicenter trial for the ability to detect Chlamydia trachomatis infections. Test performance...

Multicenter Evaluation of AMPLICOR and Automated COBAS AMPLICOR CT/NG Tests for Neisseria gonorrhoeae

Martin, David H., Cammarata, Cathy, Van Der Pol, Barbara, Jones, Robert B., Quinn, Thomas C., Gaydos, Charlotte A., ...

The fully automated COBAS AMPLICOR CT/NG and semiautomated AMPLICOR CT/NG tests were evaluated in a multicenter trial for their ability to detect Neisseria gonorrhoeae infections. Test performance...

Evaluation of Nucleic Acid Amplification Tests as Reference Tests for Chlamydia trachomatis Infections in Asymptomatic Men

Johnson, Robert E., Green, Timothy A., Schachter, Julius, Jones, Robert B., Hook, Edward W., Black, Carolyn M., ...

Urine ligase chain reaction (LCR) and PCR tests and urethral swab culture were compared for their abilities to detect Chlamydia trachomatis infection in 3,639 asymptomatic men by using one-, two-,...

Enhancing the Specificity of the COBAS AMPLICOR CT/NG Test for Neisseria gonorrhoeae by Retesting Specimens with Equivocal Results

Van Der Pol, Barbara, Martin, David H., Schachter, Julius, Quinn, Thomas C., Gaydos, Charlotte A., Jones, Robert B., ...

The COBAS AMPLICOR CT/NG test for Neisseria gonorrhoeae cross-reacts with certain strains of nonpathogenic Neisseria species. In some strains, the target sequence is identical to that of N....

Head-to-Head Multicenter Comparison of DNA Probe and Nucleic Acid Amplification Tests for Chlamydia trachomatis Infection in Women Performed with an Improved Reference Standard

Black, Carolyn M., Marrazzo, Jeanne, Johnson, Robert E., Hook III, Edward W., Jones, Robert B., Green, Timothy A., ...

Few evaluations of tests for Chlamydia trachomatis have compared nucleic acid amplification tests (NAATs) with diagnostic tests other than those by culture. In a five-city study of 3,551 women, we...

Vaginal Swabs Are Appropriate Specimens for Diagnosis of Genital Tract Infection with Chlamydia trachomatis

Schachter, Julius, McCormack, William M., Chernesky, Max A., Martin, David H., Van Der Pol, Barbara, Rice, Peter A., ...

Because self-collected vaginal swabs (VS) are potentially very useful for screening asymptomatic women for Chlamydia trachomatis infection, a multicenter study evaluated that specimen with nucleic...

An Intravaginal Live Candida Challenge in Humans Leads to New Hypotheses for the Immunopathogenesis of Vulvovaginal Candidiasis

Fidel, Paul L., Barousse, Melissa, Espinosa, Terri, Ficarra, Mercedes, Sturtevant, Joy, Martin, David H., ...

Acute and recurrent vulvovaginal candidiasis (VVC) remains a significant problem in women of childbearing age. While clinical studies of women with recurrent VVC (RVVC) and animal models have...

Population-Based Genetic and Evolutionary Analysis of Chlamydia trachomatis Urogenital Strain Variation in the United States†

Millman, Kim, Black, Carolyn M., Johnson, Robert E., Stamm, Walter E., Jones, Robert B., Hook, Edward W., ...

Chlamydia trachomatis is a major cause of ocular and sexually transmitted diseases worldwide. While much of our knowledge about its genetic diversity comes from serotyping or ompA genotyping, no...

Field Studies of an Attenuated Venezuelan Equine Encephalomyelitis Vaccine (Strain TC-83)

Eddy, Gerald A., Martin, David H., Reeves, William C., Johnson, Karl M.

A series of field studies using strain TC-83 attenuated Venezuelan equine encephalomyelitis vaccine in horses was made to determine the rate of seroconversions, the postvaccination viremia, and the...

Use of Multiple Nucleic Acid Amplification Tests To Define the Infected-Patient “Gold Standard” in Clinical Trials of New Diagnostic Tests for Chlamydia trachomatis Infections

Martin, David H., Nsuami, Malanda, Schachter, Julius, Hook, Edward W., Ferrero, Dennis, Quinn, Thomas C., ...

Nucleic acid amplification tests (NAATs) can be used to define the infected-patient “gold standard” for the purpose of designing studies of the performance of Chlamydia trachomatis diagnostic...

Single-Nucleotide Polymorphisms in the rRNA Operon and Variable Numbers of Tandem Repeats in the Lipoprotein Gene among Mycoplasma genitalium Strains from Clinical Specimens

Ma, Liang, Martin, David H.

We characterized the single-nucleotide polymorphisms in the rRNA operon and variable numbers of tandem repeats in the lipoprotein gene MG309 among Mycoplasma genitalium strains from clinical...

Use of Species-Directed 16S rRNA Gene PCR Primers for Detection of Atopobium vaginae in Patients with Bacterial Vaginosis

Ferris, Michael J., Masztal, Alicia, Martin, David H.

Recent studies suggest that the association between a metronidazole-resistant anaerobe, Atopobium vaginae, and bacterial vaginosis (BV) warrants further investigation. In the present study, specific...

Impact of Patient Characteristics on Performance of Nucleic Acid Amplification Tests and DNA Probe for Detection of Chlamydia trachomatis in Women with Genital Infections

Marrazzo, Jeanne M., Johnson, Robert E., Green, Timothy A., Stamm, Walter E., Schachter, Julius, Bolan, Gail, ...

The performance of nucleic acid amplified tests (NAAT) for Chlamydia trachomatis at the cervix and in urine was examined in 3,551 women, and the impacts of clinical findings (age, endocervical and...

Sequence-Based Typing of Mycoplasma genitalium Reveals Sexual Transmission†

Hjorth, Sofie Vetli, Björnelius, Eva, Lidbrink, Peter, Falk, Lars, Dohn, Birthe, Berthelsen, Lene, ...

Mycoplasma genitalium causes male nonchlamydial, nongonococcal urethritis and is associated with cervicitis and pelvic inflammatory disease in women. Epidemiological studies indicate that M....

Cultivation-Independent Analysis of Changes in Bacterial Vaginosis Flora Following Metronidazole Treatment▿

Ferris, Michael J., Norori, Johana, Zozaya-Hinchliffe, Marcela, Martin, David H.

PCR was used to survey bacterial vaginosis flora before and after metronidazole treatment. The species composition for pretreatment patients was variable. Lactobacillus iners was prominent in all...

Mycoplasma genitalium: an efficient strategy to generate genetic variation from a minimal genome

Ma, Liang, Jensen, Jørgen S, Myers, Leann, Burnett, Judy, Welch, Mary, Jia, Qiuyao, ...

Mycoplasma genitalium, a human pathogen associated with sexually transmitted diseases, is unique in that it has smallest genome of any known free-living organism. The goal of this study was to...

Double-Blind Placebo-Controlled Treatment Trial of Chlamydia trachomatis Endocervical Infections in Pregnant Women

Martin, David H., Eschenbach, David A., Cotch, Mary Frances, Nugent, Robert P., Rao, A. Vijaya, Klebanoff, Mark A., ...

Objective: The purpose of this study was to determine if treatment of pregnant women with Chlamydia trachomatis infection would lower the incidence of preterm delivery and/or low birth weight.

Prevalence and Abundance of Uncultivated Megasphaera-Like Bacteria in the Human Vaginal Environment▿

Zozaya-Hinchliffe, Marcela, Martin, David H., Ferris, Michael J.

Cultivation-independent analysis of 16S rRNA gene sequences in vaginal samples revealed two previously unrecognized, uncultivated Megasphaera-like phylotypes. Phylogenetic analysis and environmental...

Mycoplasma genitalium-Encoded MG309 Activates NF-κB via Toll-Like Receptors 2 and 6 To Elicit Proinflammatory Cytokine Secretion from Human Genital Epithelial Cells▿

McGowin, Chris L., Ma, Liang, Martin, David H., Pyles, Richard B.

Mycoplasma genitalium has been implicated in several important reproductive tract syndromes in women, including pelvic inflammatory disease, cervicitis, and tubal factor infertility. The mechanisms...