David H. Phillips

Mutagenicity testing for chemical risk assessment: update of the WHO/IPCS Harmonized Scheme (2009)

Eastmond, David A., Hartwig, Andrea, Anderson, Diana, Anwar, Wagida A., Cimino, Michael C., Dobrev, Ivan, ...

Since the publication of the International Programme on Chemical Safety (IPCS) Harmonized Scheme for Mutagenicity Testing, there have been a number of publications addressing test strategies for...

The TERT-CLPTM1L lung cancer susceptibility variant associates with higher DNA adduct formation in the lung (2009)

Zienolddiny, Shanbeh, Skaug, Vidar, Landvik, Nina E., Ryberg, David, Phillips, David H., Houlston, Richard, ...

Genome-wide association studies have provided evidence that common variation at 5p15.33 [telomerase reverse transcriptase (TERT)-cleft lip and palate transmembrane 1-like (CLPTM1L)], 6p21.33 and...

Metabolic activation of benzo[a]pyrene in vitro by hepatic cytochrome P450 contrasts with detoxification in vivo: experiments with hepatic cytochrome P450 reductase null mice (2008)

Arlt, Volker M., Stiborová, Marie, Henderson, Colin J., Thiemann, Markus, Frei, Eva, Aimová, Dagmar, ...

Many studies using mammalian cellular and subcellular systems have demonstrated that polycyclic aromatic hydrocarbons, including benzo[a]pyrene (BaP), are metabolically activated by cytochrome P450s...

DNA adducts and cancer risk in prospective studies: a pooled analysis and a meta-analysis (2008)

Veglia, Fabrizio, Loft, Steffen, Matullo, Giuseppe, Peluso, Marco, Munnia, Armelle, Perera, Frederica, ...

Bulky DNA adducts are biomarkers of exposure to aromatic compounds and of the ability of the individual to metabolically activate carcinogens and to repair DNA damage. Their ability to predict cancer...

Genotoxicity of 3-nitrobenzanthrone and 3-aminobenzanthrone in MutaTMMouse and lung epithelial cells derived from MutaTMMouse (2008)

Arlt, Volker M., Gingerich, John, Schmeiser, Heinz H., Phillips, David H., Douglas, George R., White, Paul A.

FE1 lung epithelial cells derived from Muta™Mouse are a new model system to provide in vitro mutagenicity data with the potential to predict the outcome of an in vivo Muta™Mouse test....

p53-dependent global nucleotide excision repair of cisplatin-induced intrastrand cross links in human cells (2008)

Bhana, Sara, Hewer, Alan, Phillips, David H., Lloyd, Daniel R.

Cisplatin is an extremely effective chemotherapeutic agent used for the treatment of testicular and other solid tumours. It induces a variety of structural modifications in DNA, the most abundant...

Identification through microarray gene expression analysis of cellular responses to benzo(a)pyrene and its diol-epoxide that are dependent or independent of p53 (2008)

Hockley, Sarah L., Arlt, Volker M., Jahnke, Gunnar, Hartwig, Andrea, Giddings, Ian, Phillips, David H.

Human colon carcinoma cells (HCT116) differing in p53 status were exposed to benzo(a)pyrene (BaP) or anti-benzo(a)pyrene-trans-7,8-dihydrodiol-9,10-epoxide (BPDE) and their gene expression responses...

Aristolochic acid mutagenesis: molecular clues to the aetiology of Balkan endemic nephropathy-associated urothelial cancer (2007)

Arlt, Volker M., Stiborová, Marie, Vom Brocke, Jochen, Simões, Maria L., Lord, Graham M., Nortier, Joelle L., ...

Balkan endemic nephropathy (BEN) is found in certain rural areas of the Balkans and affects at least 25 000 inhabitants. Of the many hypotheses on BEN, the Aristolochia hypothesis has recently...

Mutagenicity and DNA Adduct Formation by the Urban Air Pollutant 2-Nitrobenzanthrone (2007)

Arlt, Volker M., Glatt, Hansruedi, Gamboa Da Costa, Gonçalo, Reynisson, Jóhannes, Takamura-Enya, Takeji, Phillips, David H.

2-Nitrobenzanthrone (2-NBA) has recently been detected in ambient air particulate matter. Its isomer 3-nitrobenzanthrone (3-NBA) is a potent mutagen and suspected human carcinogen identified in...

Formation and persistence of DNA adducts formed by the carcinogenic air pollutant 3-nitrobenzanthrone in target and non-target organs after intratracheal instillation in rats (2007)

Bieler, Christian A., Cornelius, Michael G., Stiborova, Marie, Arlt, Volker M., Wiessler, Manfred, Phillips, David H., ...

Sprague–Dawley rats were treated by intratracheal instillation with a single dose of 0.2 mg/kg body wt of 3-nitrobenzanthrone (3-NBA), and whole blood, lungs, pancreases, kidneys, urinary bladders,...

Growth kinetics in MCF-7 cells modulate benzo[a]pyrene-induced CYP1A1 up-regulation. (2007)

Jiao, Haiyan, Allinson, Sarah L., Walsh, Michael J., Hewitt, Rebecca, Cole, Kathy J., Phillips, David H., ...

Pro-carcinogens, such as benzo[a]pyrene (B[a]P), that are exogenous ligands of the aromatic hydrocarbon receptor may influence the susceptibility of target-cell populations through the up-regulation...

Growth kinetics in MCF-7 cells modulate benzo[a]pyrene-induced CYP1A1 up-regulation (2007)

Jiao, Haiyan, Allinson, Sarah L., Walsh, Michael J., Hewitt, Rebecca, Cole, Kathy J., Phillips, David H., ...

Pro-carcinogens, such as benzo[a]pyrene (B[a]P), that are exogenous ligands of the aromatic hydrocarbon receptor may influence the susceptibility of target-cell populations through the up-regulation...

Time- and concentration-dependent changes in gene expression induced by benzo(a)pyrene in two human cell lines, MCF-7 and HepG2 (2006)

Hockley, Sarah L, Arlt, Volker M, Brewer, Daniel, Giddings, Ian, Phillips, David H

Abstract Background The multi-step process of carcinogenesis can be more fully understood by characterizing gene expression changes induced in cells by carcinogens. In this study, expression...

Polymorphisms of DNA repair genes and risk of non-small cell lung cancer (2006)

Zienolddiny, Shanbeh, Campa, Daniele, Lind, Helge, Ryberg, David, Skaug, Vidar, Stangeland, Lodve, ...

Lung cancer is a leading cause of cancer mortality with an inter-individual difference in susceptibility to the disease. The inheritance of low-efficiency genotypes involved in DNA repair and...

A biomarker model of sublethal genotoxicity (DNA single-strand breaks and adducts) using the sentinel organism Aporrectodea longa in spiked soil. (2005)

Martin, Francis L., Piearce, Trevor G., Hewer, Alan, Phillips, David H., Semple, Kirk T.

There is a need to develop risk biomarkers during the remediation of contaminated land. We employed the earthworm, Aporrectodea longa (Ude), to determine whether genotoxicity measures could be...

Organ specificity of DNA adduct formation by tamoxifen and {alpha}-hydroxytamoxifen in the rat: implications for understanding the mechanism(s) of tamoxifen carcinogenicity and for human risk assessment (2005)

Phillips, David H., Hewer, Alan, Osborne, Martin R., Cole, Kathleen J., Churchill, Cyd, Arlt, Volker M.

Tamoxifen is an anti-oestrogen widely used in the adjuvant therapy of breast cancer and is also used as a prophylactic to prevent the disease in high-risk women. An increased risk of endometrial...

Early proximal tubule injury in experimental aristolochic acid nephropathy: functional and histological studies (2005)

Lebeau, Catherine, Debelle, Frédéric D., Arlt, Volker M., Pozdzik, Agnieszka, De Prez, Eric G., Phillips, David H., ...

Background. Aristolochic acid (AA), the plant extract of Aristolochia species, is involved in the onset of progressive tubulointerstitial renal fibrosis in humans. Clinical and in vitro findings have...

Hepatic DNA adduct dosimetry in rats fed tamoxifen: a comparison of methods (2005)

Schild, Laura J., Phillips, David H., Osborne, Martin R., Hewer, Alan, Beland, Frederick A., Churchwell, Mona I., ...

Liver homogenates from rats fed tamoxifen (TAM) in the diet were shared among four different laboratories. TAM–DNA adducts were assayed by high pressure liquid chromatography–electrospray tandem...

Polymorphisms of DNA repair genes and risk of non-small cell lung cancer (2005)

Zienolddiny, Shanbeh, Campa, Daniele, Lind, Helge, Ryberg, David, Skaug, Vidar, Stangeland, Lodve, ...

Lung cancer is a leading cause of cancer mortality with an interindividual difference in susceptibility to the disease. The inheritance of low-efficiency genotypes involved in DNA repair and...

Hepatic DNA adduct dosimetry in rats fed tamoxifen: a comparison of methods (2005)

Schild, Laura J., Phillips, David H., Osborne, Martin R., Hewer, Alan, Beland, Frederick A., Churchwell, Mona I., ...

Liver homogenates from rats fed tamoxifen (TAM) in the diet were shared among four different laboratories. TAM-DNA adducts were assayed by high pressure liquid chromatography-electrospray tandem mass...

Organ specificity of DNA adduct formation by tamoxifen and {alpha}-hydroxytamoxifen in the rat: implications for understanding the mechanism(s) of tamoxifen carcinogenicity and for human risk assessment (2005)

Phillips, David H., Hewer, Alan, Osborne, Martin R., Cole, Kathleen J., Churchill, Cyd, Arlt, Volker M.

Tamoxifen is an anti-oestrogen widely used in the adjuvant therapy of breast cancer and is also used as a prophylactic to prevent the disease in high-risk women. An increased risk of endometrial...

Early proximal tubule injury in experimental aristolochic acid nephropathy: functional and histological studies (2005)

Lebeau, Catherine, Debelle, Frédéric D., Arlt, Volker M., Pozdzik, Agnieszka, De Prez, Eric G., Phillips, David H., ...

Background. Aristolochic acid (AA), the plant extract of Aristolochia species, is involved in the onset of progressive tubulointerstitial renal fibrosis in humans. Clinical and in vitro findings have...

Modulation of N-methyl-N-nitrosourea-induced crypt restricted metallothionein immunopositivity in mouse colon by a non-genotoxic diet-related chemical (2004)

Donnelly, Eilish T., Bardwell, Helen, Thomas, Geraldine Anne, Williams, E. Dillwyn, Hoper, Margaret, Crowe, Paul, ...

Red meat consumption is associated with endogenous metabolic generation of mutagenic N-nitroso compounds (NOC) and may be implicated in causation of colorectal cancer. Assessment of a biologically...

Modulation of N-methyl-N-nitrosourea (MNU) induced crypt restricted metallothionein (MT) immunopositivity in mouse colon by a non-genotoxic diet related chemical (2004)

Donnelly, Eilish T., Bardwell, Helen, Thomas, Geraldine Anne, Williams, E. Dillwyn, Hoper, Margaret, Crowe, Paul, ...

Red meat consumption is associated with endogenous metabolic generation of mutagenic N-nitroso compounds (NOC) and may be implicated in causation of colorectal cancer (CRC). Assessment of a...

Activation of 3-nitrobenzanthrone and its metabolites to DNA-damaging species in human B lymphoblastoid MCL-5 cells (2004)

Arlt, Volker M., Cole, Kathleen J., Phillips, David H.

3‐Nitrobenzanthrone (3‐NBA) is one of the most potent mutagens in the Ames Salmonella typhimurium assay and a suspected human carcinogen recently identified in diesel exhaust and in...

Electrospray Ionization–Tandem Mass Spectrometry and 32P-Postlabeling Analyses of Tamoxifen–DNA Adducts in Humans (2004)

Beland, Frederick A., Churchwell, Mona I., Doerge, Daniel R., Parkin, Daniel R., Malejka-Giganti, Danuta, Hewer, Alan, ...

Background: Although the nonsteroidal antiestrogen tamoxifen is used as an adjuvant chemotherapeutic agent to treat hormone-dependent breast cancer and as a chemopreventive agent in women with...

Metabolic activation of the environmental contaminant 3-nitrobenzanthrone by human acetyltransferases and sulfotransferase (2002)

Arlt, Volker M., Glatt, Hansruedi, Muckel, Eva, Pabel, Ulrike, Sorg, Bernd L., Schmeiser, Heinz H., ...

3-Nitrobenzanthrone (3-NBA) an extremely potent mutagen and suspected human carcinogen identified in diesel exhaust and in airborne particulate matter was shown to form multiple DNA adducts in vitro...

Associations between carcinogen-DNA damage, glutathione S-transferase genotypes, and risk of lung cancer in the prospective Physicians' Health Cohort Study (2002)

Perera, Frederica P., Mooney, LaVerne A., Stampfer, Meir, Phillips, David H., Bell, Douglas A., Rundle, Andrew, ...

DNA damage from polycyclic aromatic hydrocarbons (PAH) and other aromatic/hydrophobic compounds has been implicated in case–control studies as a risk factor for lung cancer, as have common...

Morphological transformation of C3H/M2 mouse fibroblasts by, and genotoxicity of, extracts of human milk. (2001)

Pfau, Wolfgang, Martin, Francis L., Cole, Kathleen J., Weaver, Gillian, Marquardt, Hans, Phillips, David H., ...

Breast cancer may be initiated by environmental/dietary agents and human milk may act as an ex vivo indicator of in vivo exposure of mammary epithelial cells to genotoxins. Extracts of human milk...

Genotoxicity of human breast milk from different countries. (2001)

Martin, Francis L., Cole, Kathleen J., Weaver, Gillian, Hong, Ga S., Lam, Barbara C., Balaram, Prabha, ...

Dietary and/or environmental factors appear to play a key role in the international variations that exist in breast cancer incidence. The genotoxicity of breast milk extracts is being examined as a...

Understanding the genotoxicity of tamoxifen? (2001)

Phillips, David H.

Tamoxifen is an anti-oestrogenic drug widely used for adjuvant therapy of breast cancer. Its use has caused an increased incidence of endometrial cancer and it is also a potent carcinogen in rat...

Genotoxicity of human breast milk from different countries (2001)

Martin, Francis L., Cole, Kathleen J., Weaver, Gillian, Hong, Ga S., Lam, Barbara C., Balaram, Prabha, ...

Dietary and/or environmental factors appear to play a key role in the international variations that exist in breast cancer incidence. The genotoxicity of breast milk extracts is being examined as a...

The influence of dietary and environmental factors on prostate cancer risk. (2000)

Kooiman, G., Martin, Francis L., Williams, J. A., Grover, Philip L., Phillips, David H., Muir, Gordon H.

Prostate cancer is the most common malignancy found in males and one of the most common causes of cancer death. The epidemiology implicates environmental and nutritional factors in the initiation and...

Activation of genotoxins to DNA-damaging species in exfoliated breast milk cells. (2000)

Martin, Francis L., Cole, K. J., Williams, J. A., Millar, B. C., Harvey, D. R., Weaver, Gillian, ...

Exfoliated cells, isolated from breast milk samples donated by UK-resident women (n=15), were incubated, either immediately or after culture for 7 days, with one of a series of genotoxins, either in...

Human prostate tissue expresses CYP1A2 mNRA transcripts and metabolically activates potential prostate carcinogens. (2000)

Williams, J. A., Martin, Francis L., Muir, Gordon H., Grover, Philip L., Phillips, David H.

Epidemiological evidence suggests a link between meat consumption and prostate cancer. In this study, benign prostatic hyperplasia tissues, obtained by transurethral resection or radical retropubic...

DNA damage in breast milk cells and its induction by "early" and "late" milk extracts. (2000)

Martin, Francis L., Cole, Kathleen J., Harvey, D. R., Weaver, Gillian, Williams, J. A., Millar, B. C., ...

Environmental and dietary factors are thought to be significant in breast cancer aetiology. The alkaline single-cell gel electrophoresis (‘Comet’) assay was used to examine breast milk cells for...

Lack of evidence for tamoxifen- and toremifene-DNA adducts in lymphocytes of treated patients (2000)

Bartsch, Helmut, Phillips, David H., Nair, Jagadeesan, Hewer, Alan, Meyberg-Solomeyer, Gabriele, Grischke, Eva-Maria

Tamoxifen (TAM) is used for the adjuvant treatment of women with breast cancer and has also been recommended as a chemopreventive agent. Among unwanted side effects, TAM was shown to increase...

Pathways of heterocyclic amine activation in the breast: DNA adducts of 2-amino-3-methylimidazo[4,5-f]quinoline (IQ) formed by peroxidases and in human mammary epithelial cells and fibroblasts (2000)

Williams, J.Andrew, Stone, Elaine M., Millar, Barbara C., Hewer, Alan, Phillips, David H.

Most human mammary carcinomas originate in the epithelial cells of the breast ducts. A potential role of heterocyclic amines (HAs) in the aetiology of this disease has led us to investigate...

Metabolic proficiency and benzo[a]pyrene DNA adduct formation in APCMin mouse adenomas and uninvolved mucosa (1999)

Sattar, Abid, Hewer, Alan, Phillips, David H., Campbell, Frederick C.

Tumour formation may involve interactions between genetic factors and environmental carcinogens. Adenoma formation in APCMin/+ mice is associated homozygous adenomatous polyposis coli (APC) gene...

Heterocyclic aromatic amines induce DNA strand breaks and cell transformation (1999)

Pfau, Wolfgang, Martin, Francis L., Cole, Kathleen J., Venitt, Stanley, Phillips, David H., Grover, Philip L., ...

Heterocyclic aromatic amines (HAAs), formed during the cooking of foods, are known to induce tumours in rodent bioassays and may thus contribute to human cancer risk. We tested six HAAs in a...

Idoxifene derivatives are less reactive to DNA than tamoxifen derivatives, both chemically and in human and rat liver cells (1999)

Osborne, Martin R., Hewer, Alan, Davis, Warren, Strain, Alastair J., Keogh, Adrian, Hardcastle, Ian R., ...

The drug tamoxifen shows evidence of genotoxicity, and induces liver tumours in rats. Covalent DNA adducts have been detected in the liver of rats treated with tamoxifen, and these arise through...

Lack of evidence from HPLC 32P-post-labelling for tamoxifen–DNA adducts in the human endometrium (1999)

Carmichael, Paul L., Sardar, Shahida, Crooks, Neil, Neven, Patrick, Van Hoof, Ingrid, Ugwumadu, Austin, ...

Tamoxifen is associated with an increased incidence of endometrial cancer in women. It is also a potent carcinogen in rat liver and forms covalent DNA adducts in this tissue. A previous study...

Remote Time Transfer System with Epoch Pulse. (1998)

Phillips,David H., Phillips,Ruth E.

The patent describes a passive method and equipment for synchronizing time systems, atomic or precision clocks, by transferring both time and frequency information. The time and frequency information...

NDE X-Ray Computed Tomography Applications Research. (1998)

Neel, S. T., Yancey, Robert N., Eliasen, Dennis S., Phillips, David H.

This report summarizes research efforts in X-ray computed tomography at Wright Laboratory. Attention is focused on applications development efforts that have been successful in coupling CT with...

X-ray Computed Tomography for the Testing and Evaluation of Ceramic Processes. (1998)

Phillips, David H., Lannutti, John J.

X-ray computed tomography (CT), a nondestructive evaluation technique, generates high-resolution, three-dimensional density profiles of geometrically complex objects. One use of CT is characterizing...

SHORT COMMUNICATION: Metabolic activation and DNA binding of food mutagens and other environmental carcinogens in human mammary epithelial cells (1996)

Carmichael, Paul L., Stone, Elaine M., Grover, Phillip L., Gusterson, Barry A., Phillips, David H.

Cultures of human mammary epithelial cells were treated with one of seven heterocyclic amine food mutagens [2-amino-3-methylimidazo[4, 5-f)quinoline (IQ), 2-amino-3, 4-dimethylimidazo[4,...

p53mutations in lung tumours: relationship to gender and lung DNA adduct levels (1996)

Kure, Elin H., Ryberg, David, Hewer, Alan, Phillips, David H., Skaug, Vidar, Bæera, Rita, ...

Human lung cancer exhibits a high frequency of transver-sion mutations at G: C base pairs of the p53 gene, possibly the result of DNA damage by cigarette smoke constituents, most notably...

Activation of tamoxifen and its metabolite {alpha}-hydroxytamoxifen to DNA-binding products: comparisons between human, rat and mouse hepatocytes (1996)

Phillips, David H., Carmichael, Paul L., Hewer, Alan, Cole, Kathleen J., Hardcastle, Ian R., Poon, Grace K., ...

The metabolic activation of tamoxifen and its metabolite α-hydroxytamoxifen in primary cultures of rat, mouse and human hepatocytes has been compared. The extent of formation of DNA adducts in these...

Tamoxifen does not form detectable DNA adducts in white blood cells of breast cancer patients (1996)

Phillips, David H., Hewer, Alan, Grover, Philip L., Poon, Grace K., Carmichael, Paul L.

DNA from white blood cells of seven women receiving tamoxifen as adjuvant therapy for breast cancer and of three women who served as healthy controls was analysed for the presence of tamoxifen-DNA...

Reduced genotoxicity of [D5-ethyl]-tamoxifen implicates {alpha}-hydroxylation of the ethyl group as a major pathway of tamoxifen activation to a liver carcinogen (1994)

Phillips, David H., Potter, Gerard A., Horton, Martin N., Hewker, Alan, Crofton-Sleigh, Christopher, Jarman, Michael, ...

A proposed mechanism for the metabolic activation of tamoxifen to electrophilk species that form DNA adducts leading to liver cancer involves α-hydroxylation of the ethyl group in the critical first...

Co-chromatography of a tamoxifen epoxide-deoxyguanylic acid adduct with a major DNA adduct formed in the livers of tamoxifen-treated rats (1994)

Phillips, David H., Hewer, Alan, White, Ian N. H., Farmer, Peter B.

Tamoxifen is a potent liver carcinogen in rats and has been shown to form covalent DNA adducts in the livers of several species of rodent. We have shown previously by 32P-postlabelling...

Genotoxicity characteristics of reverse diol-epoxides of chrysene (1993)

Glatt, Hansruedi, Wameling, Christa, Elsberg, Sabine, Thomas, Helmut, Marquardt, Hans, Hewer, Alan, ...

Trans-3,4-dihydroxy-3,4-dihydrochrysene (chrysene-3,4-diol), a major metabolite of chrysene, is further metabolized by rat liver enzymes to products which effectively revert the his− Salmonella...

Determination of target nucleotides involved in 7-methoxy-2-nitronaphtho[2,1-b]furan (R7000)-DNA adduct formation (1993)

Touati, Eliette, Phillips, David H., Quillardet, Philippe, Hofnung, Maurice

The characterization of target nucleotides involved in the binding to DNA of 7-methoxy-2-nitro-naphtho[2,1–b]furan (R7000), a very potent genotoxic nitrofuran derivative, was investigated. Since...

Detection and characterization by 32P-postlabelling of DNA adducts induced by a Fenton-type oxygen radical-generating system (1992)

Carmichael, Paul L., Shé, Meadhbh Ni, Phillips, David H.

Reactive oxygen species can give rise to numerous modifications of DNA. We have investigated the formation of such modifications using the nuclease P1 digestion method of the 32P-postlabelling...

Interaction of omeprazole with DNA in rat tissues (1992)

Phillips, David H., Hewer, Alan, Osborne, Martin R.

There has been concern recently over the possible genotoxicity of omeprazole, a potent inhibitor of gastric acid secretion in humans. In order to investigate its possible DNA binding activity in...

Genotoxic potential of tamoxifen and analogues in female Fischer F344/n rats, DBA/2 and C57BL/6 mice and in human MCL-5 cells (1992)

White, Ian N.H., De Matteis, Francesco, Davies, Adrian, Smith, Lewis L., Crofton-Sleigh, Christopher, Venitt, Stanley, ...

Chronic administration of tamoxifen to female rats causes hepatocellular carcinomas. We have investigated damage to liver DNA caused by the administration of tamoxifen to female Fischer F344/N...

Metabolic activation of the food mutagens 2-amino-3-methylimidazo[4,5-f]quinoline (IQ) and 2-amino-3,4-dimethylimidazo[4,5-f]quinoline (MeIQ) to DNA binding species in human mammary epithelial cells (1992)

Pfau, Wolfgang, O'Hare, Michael J., Grover, Philip L., Phillips, David H.

When incubated in suspension with the heterocyclic aromatic amine food mutagens 2-amino-3-methylimidazo [4,5-f]quinoline (IQ) and 2-amino-3,4-dimethylimidazo[4,5-f]quinoline (MeIQ), human mammary...

Evaluation of DNA-binding activity of hydroxyanthraquinones occurring in Rubia tinctorum L (1991)

Poginsky, Barbara, Westendorf, Johannes, Blömeke, Brunhilde, Marquardt, Hans, Hewer, Alan, Grover, Philip L., ...

The naturally-occurring anthraquinones (AQs), alizarin (1,2-dihydroxyanthraquinone) and lucidin (1,3-dihydroxy-2-hydroxymethylanthraquinone), were incubated with DNA in the presence of S9 mix. The...

The metabolic activation of dibenz[a,h]anthracene in mouse skin examined by 32P-postlabelling: minor contribution of the 3,4-diol 1,2-oxides to DNA binding (1991)

Lecoq, Sophie, Ni She, Meadhbh, Hewer, Alan, Grover, Philip L., Platt, Karl L., Oesch, Franz, ...

Dibenz[a,h]anthracene (DB[a,h]A) and the related 3,4-diol and anti- and syn-3,4-diol 1,2-oxides were applied to the shaved dorsal skin of groups of four C57B1/CB1 mice. Twenty-four hours later the...

Tissue distribution of DNA adducts in CDF1 mice fed 2-amino-3-methylimidazo[4,5-f]quinoline (IQ) and 2-amino-3,4-dimethylimidazo[4,5-fquinoline (MeIQ) (1990)

Hall, Michael, Shé, Meadhbh Ni, Wild, Dieter, Fasshauer, Ingrid, Hewer, Alan, Phillips, David H.

Male and female CDF, mice were administered a single oral dose of 3 µmol of the food mutagens 2-amino-3-methyl-imidazo[4,5-f]quinoline (IQ) or 2-amino-3,4-dimethylimidazo [4,5-f]quinoline (MeIQ) and...

Analysis of the polycyclic aromatic hydrocarbon content of petrol and diesel engine lubricating oils and determination of DNA adducts in topically treated mice by 32P-postlabelling (1990)

Carmichael, Paul L., Jacob, Jürgen, Grimmer, Gernot, Phillips, David H.

Engine lubricating oils are known to accumulate carcinogenic polycyclic aromatic hydrocarbons (PAHs) during engine running. Oils from nine petrol-powered and 11 diesel-powered vehicles, in addition...

32P-Postlabelling analysis of DNA adducts in the skin of mice treated with petrol and diesel engine lubricating oils and exhaust condensates (1989)

Schoket, Bernadette, Hewer, Alan, Grover, Phillip L., Phillips, David H.

Samples of unused or used petrol and diesel engine lubricating oils were applied to the shaved dorsal skin of 4- to 6-week-old male Parkes mice, either as a single treatment (50 μ/mouse) or as four...

Enzyme-mediated phosphorylation of polycyclic hydrocarbon metabolites: detection of non-adduct compounds in the 32P-postlabelling assay (1989)

Masento, Mark S., Hewer, Alan, Grover, Philip L., Phillips, David H.

32P-Postlabelling analysis is a sensitive method of detecting covalent modification of DNA by chemical carcinogens. We demonstrate that tetrol derivatives of the polycyclk aromatic hydrocarbons...

Metabolic activation of 7-ethyl- and 7-methylbenz[a]anthracene in mouse skin (1988)

McKay, Simon, Phillips, David H., Hewer, Alan J., Grover, Philip L.

The carcinogen 7-methylbenz[a]anthracene (7-MBA) is considered to be metabolically activated via its bay-region dihydrodiol-epoxide, trans-3,4-dihydro-3,4-dihydroxy-7- methyl-benz[a]anthracene...

Further metabolism of diol-epoxides of chrysene and dibenz[a,c]anthracene to DNA binding species as evidenced by 32P-postlabelling analysis (1988)

Hall, Michael, Parker, Deborah K., Hewer, Alan J., Phillips, David H., Grover, Philip L.

Incubation of r-1t-2-dihydroxy-t-3,4-oxy-1,2,3,4-tetrahydro-chrysene (anti-chrysene-1,2-diol 3,4-oxide), the bay-region diol-epoxide of chrysene, with rat liver microsomes in the presence of NADP+...

Activating mutations in human c-Ha-ras-1 gene induced by reactive derivatives of safrole and the glutamic pyrolysis product, Glu-P-3 (1988)

Ireland, Christine M., Cooper, Colin S., Marshall, Christopher J., Hebert, Eric, Phillips, David H.

Foci of transformed NIH3T3 cells were observed after transfection of plasmids containing the c-Ha-ras-1 protooncogene modified in vitro either with the 3-N,N-acetoxyacetyl derivative (N-AcO-AGlu-P-3)...

Aromatic DNA adducts in human bone marrow and peripheral blood leukocytes (1986)

Phillips, David H., Hewer, Alan, Grover, Philip L.

DNA from normal human bone marrow mononuclear and non-mononuclear cells was analysed by 32P-post-labelling for the presence of aromatic adducts. Ten out of 10 individuals showed the presence of...

Evidence for N-(deoxyguanosin-8-yl)-1-aminopyrene as a major DNA adduct in female rats treated with 1-nitropyrene (1985)

Stanton, Caroline A., Chow, Fong L., Phillips, David H., Grover, Philip L., Garner, R. Colin, Martin, Carl N.

[3H]1-Nitropyrene was administered at a dose of 25 mg/kg by i.p. injection to female Wistar rats. Animals were killed 24h later and DNA was isolated from kidney, liver and mammary gland,...

32P-Post-labelling analysis of DNA adducts formed in the livers of animals treated with safrole, estragole and other naturally-occurring alkenylbenzenes. I. Adult female CD-1 mice (1984)

Randerath, Kurt, Haglund, Roberta E., Phillips, David H., Reddy, M. Vijayaraj

The binding of a series of alkenylbenzenes to liver DNA of adult female CD-1 mice, isolated 24 h after i.p. administration of non-radioactive test compound (2 or 10 mg/mouse), was investigated by...

32P-Post-labelling analysis of DNA adducts formed in the livers of animals treated with safrole, estragole and other naturally-occurring alkenylbenzenes. II. Newborn male B6C3F1 mice (1984)

Phillips, David H., Reddy, M. Vijayaraj, Randerath, Kurt

When a series of nine alkenylbenzenes were administered to preweanling male mice, safrole, estragole and methyleugenol induced a significant incidence of hepatic carcinomas, while eugenol, anethole,...

Repair replication characteristics of human cells exposed to 1' -acetoxysafrole or 1' -acetoxyestragole (1982)

Phillips, David H., Hanawalt, Philip C.

The response of cultured human cells to treatment with 1′ -acetoxysafrole (1′ -AcO-S) and 1′ -acetoxyestragole (1′ -AcO-E), which are electrophilic and mutagenic, has been examined. Fifty...

Alkali-sensitive sites in DNA from human cells treated with ultraviolet light, 1' -acetoxysafrole or 1' -acetoxyestragole (1982)

Phillips, David H., Hanawalt, Philip C.

The formation and repair of alkali-labile sites in the DNA of human cells treated with 254 nm u.v. light, 1′ -acetoxyestragole (1′ -AcO-E) or 1′ -acetoxysafrole (1′ -AcO-S) have been studied....

Increased p53 mutation load in nontumorous human liver of Wilson disease and hemochromatosis: Oxyradical overload diseases

Hussain, S. Perwez, Raja, Kamran, Amstad, Paul A., Sawyer, Mark, Trudel, Laura J., Wogan, Gerald N., ...

Hemochromatosis and Wilson disease (WD), characterized by the excess hepatic deposition of iron and copper, respectively, produce oxidative stress and increase the risk of liver cancer. Because the...

Increased p53 mutation load in nontumorous human liver of Wilson disease and hemochromatosis: Oxyradical overload diseases

Hussain, S. Perwez, Raja, Kamran, Amstad, Paul A., Sawyer, Mark, Trudel, Laura J., Wogan, Gerald N., ...

Hemochromatosis and Wilson disease (WD), characterized by the excess hepatic deposition of iron and copper, respectively, produce oxidative stress and increase the risk of liver cancer. Because the...