David J. Hackam

v-SNARE-dependent secretion is required for phagocytosis

Hackam, David J., Rotstein, Ori D., Sjolin, Carola, Schreiber, Alan D., Trimble, William S., Grinstein, Sergio

Phagosomes are generally believed to form by gradual apposition of the plasma membrane of leukocytes onto the surface of invading microorganisms. The internalization of the encapsulated particle is...

Functional Expression of Nramp1 In Vitro in the Murine Macrophage Line RAW264.7

Govoni, Gregory, Canonne-Hergaux, François, Pfeifer, Cheryl G., Marcus, Sandra L., Mills, Scott D., Hackam, David J., ...

Mutations at the Nramp1 locus in vivo cause susceptibility to infection by unrelated intracellular microbes. Nramp1 encodes an integral membrane protein abundantly expressed in the...

v-SNARE-dependent secretion is required for phagocytosis

Hackam, David J., Rotstein, Ori D., Sjolin, Carola, Schreiber, Alan D., Trimble, William S., Grinstein, Sergio

Phagosomes are generally believed to form by gradual apposition of the plasma membrane of leukocytes onto the surface of invading microorganisms. The internalization of the encapsulated particle is...

Functional Expression of Nramp1 In Vitro in the Murine Macrophage Line RAW264.7

Govoni, Gregory, Canonne-Hergaux, François, Pfeifer, Cheryl G., Marcus, Sandra L., Mills, Scott D., Hackam, David J., ...

Mutations at the Nramp1 locus in vivo cause susceptibility to infection by unrelated intracellular microbes. Nramp1 encodes an integral membrane protein abundantly expressed in the...

The Iron Transport Protein NRAMP2 Is an Integral Membrane Glycoprotein That Colocalizes with Transferrin in Recycling Endosomes

Gruenheid, Samantha, Canonne-Hergaux, François, Gauthier, Susan, Hackam, David J., Grinstein, Sergio, Gros, Philippe

The natural resistance associated macrophage protein (Nramp) gene family is composed of two members in mammals, Nramp1 and Nramp2. Nramp1 is expressed primarily in macrophages and mutations at this...

Rho is Required for the Initiation of Calcium Signaling and Phagocytosis by Fcγ Receptors in Macrophages

Hackam, David J., Rotstein, Ori D., Schreiber, Alan, Zhang, Wei-jian, Grinstein, Sergio

Phagocytosis of bacteria by macrophages and neutrophils is an essential component of host defense against infection. The mechanism whereby the interaction of opsonized particles with Fcγ receptors...

Host Resistance to Intracellular Infection: Mutation of Natural Resistance-associated Macrophage Protein 1 (Nramp1) Impairs Phagosomal Acidification

Hackam, David J., Rotstein, Ori D., Zhang, Wei-jian, Gruenheid, Samantha, Gros, Philippe, Grinstein, Sergio

The mechanisms underlying the survival of intracellular parasites such as mycobacteria in host macrophages remain poorly understood. In mice, mutations at the Nramp1 gene (for natural...

No Longer an Innocent Bystander: Epithelial Toll-Like Receptor Signaling in the Development of Mucosal Inflammation

Gribar, Steven C, Richardson, Ward M, Sodhi, Chhinder P, Hackam, David J

Diseases of mucosal inflammation represent important causes of morbidity and mortality, and have led to intense research efforts to understand the factors that lead to their development. It is well...

The development of animal models for the study of necrotizing enterocolitis

Sodhi, Chhinder, Richardson, Ward, Gribar, Steven, Hackam, David J.

Necrotizing enterocolitis (NEC) is the leading cause of death and long-term disability from gastrointestinal disease in preterm infants, and is characterized by acute and chronic intestinal...

One-Dimensional Elastic Continuum Model of Enterocyte Layer Migration

Mi, Qi, Swigon, David, Rivière, Béatrice, Cetin, Selma, Vodovotz, Yoram, Hackam, David J.

Necrotizing enterocolitis is the leading cause of death from gastrointestinal disease in preterm infants. It results from an injury to the mucosal lining of the intestine, leading to translocation of...

Interferon-γ inhibits enterocyte migration by reversibly displacing connexin43 from lipid rafts

Leaphart, Cynthia L., Dai, Shipan, Gribar, Steven C., Richardson, Ward, Ozolek, John, Shi, Xia-hua, ...

Necrotizing enterocolitis (NEC) is associated with the release of interferon-γ (IFN) by enterocytes and delayed intestinal restitution. Our laboratory has recently demonstrated that IFN inhibits...