David J. Lefer

Protective ischaemia in patients: preconditioning and postconditioning (2009)

Granfeldt, Asger, Lefer, David J., Vinten-Johansen, Jakob

Infarct size can be limited by reducing the determinants of infarct size or increasing collateral blood flow by treatment initiated before the ischaemic event. Reperfusion is the definitive treatment...

Myocardial protection by nitrite (2009)

Calvert, John W., Lefer, David J.

Nitrite has long been considered to be an inert oxidative metabolite of nitric oxide (NO). Recent work, however, has demonstrated that nitrite represents an important tissue storage form of NO that...

Cardiomyocyte-specific overexpression of NO synthase-3 protects against myocardial ischemia-reperfusion injury (2006)

Greer, James J. M, Bryan, Nathan S, Langston, Will, Szot, Jeffrey F, Gebregzlabher, Henock, ...

OBJECTIVE: The protective effect of NO synthase-3 (eNOS)-derived NO in limiting myocardial ischemia-reperfusion (MI-R) injury is well established. We reported previously that systemic genetic...

HMG-CoA reductase inhibitors promote cholesterol-dependent Akt/PKB translocation to membrane domains in endothelial cells (2003)

Skaletz-Rorowski, Adriane, Lutchman, Mohini, Kureishi, Yasuko, Lefer, David J., Faust, Jerry R., Walsh, Kenneth

Objective: Recent results have shown that 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase inhibitors referred to as statins rapidly activate the protein kinase Akt/PKB in endothelial cells...

The role of nitric oxide and cell adhesion molecules on the microcirculation in ischaemia-reperfusion (1996)

Lefer, Allan M., Lefer, David J.

The microcirculation undergoes a profound degree of endothelial dysfunction within minutes (i.e., 2.5 to 5 min) following reperfusion of ischaemic vasculature. This has been documented in the...

Heart-targeted overexpression of caspase3 in mice increases infarct size and depresses cardiac function

Condorelli, Gianluigi, Roncarati, Roberta, Ross, John, Pisani, Angela, Stassi, Giorgio, Todaro, Matilde, ...

Up-regulation of proapoptotic genes has been reported in heart failure and myocardial infarction. To determine whether caspase genes can affect cardiac function, a transgenic mouse was generated....

CD8+-T-Cell Depletion Ameliorates Circulatory Shock in Plasmodium berghei-Infected Mice

Chang, Wun-Ling, Jones, Steven P., Lefer, David J., Welbourne, Tomas, Sun, Guang, Yin, Lijia, ...

The Plasmodium berghei-infected mouse model is a well-recognized model for human cerebral malaria. Mice infected with P. berghei exhibit (i) metabolic acidosis (pH < 7.3) associated with elevated...

Sexual dimorphism in reduced-size liver ischemia and reperfusion injury in mice: Role of endothelial cell nitric oxide synthase

Harada, Hirohisa, Pavlick, Kevin P., Hines, Ian N., Lefer, David J., Hoffman, Jason M., Bharwani, Sulaiman, ...

We have recently reported that female mice are protected to a much greater extent from the injurious effects of reduced-size liver ischemia and reperfusion (RSL+I/R) than are males by an...

Endothelial nitric oxide synthase overexpression attenuates congestive heart failure in mice

Jones, Steven P., Greer, James J. M., Van Haperen, Rien, Duncker, Dirk J., De Crom, Rini, Lefer, David J.

Congestive heart failure results in cardiovascular dysfunction and diminished vascular nitric oxide (NO) production. We hypothesized that overexpression of endothelial NO synthase (eNOS) within the...

The MEK1–ERK1/2 signaling pathway promotes compensated cardiac hypertrophy in transgenic mice

Bueno, Orlando F., De Windt, Leon J., Tymitz, Kevin M., Witt, Sandra A., Kimball, Thomas R., Klevitsky, Raisa, ...

Members of the mitogen-activated protein kinase (MAPK) cascade such as extracellular signal-regulated kinase (ERK), c-Jun N-terminal kinase (JNK) and p38 are implicated as important regulators of...

Cytoprotective effects of nitrite during in vivo ischemia-reperfusion of the heart and liver

Duranski, Mark R., Greer, James J.M., Dejam, Andre, Jaganmohan, Sathya, Hogg, Neil, Langston, William, ...

Nitrite represents a circulating and tissue storage form of NO whose bioactivation is mediated by the enzymatic action of xanthine oxidoreductase, nonenzymatic disproportionation, and reduction by...

Heart-targeted overexpression of caspase3 in mice increases infarct size and depresses cardiac function

Condorelli, Gianluigi, Roncarati, Roberta, Ross, John, Pisani, Angela, Stassi, Giorgio, Todaro, Matilde, ...

Up-regulation of proapoptotic genes has been reported in heart failure and myocardial infarction. To determine whether caspase genes can affect cardiac function, a transgenic mouse was generated....

CD8+-T-Cell Depletion Ameliorates Circulatory Shock in Plasmodium berghei-Infected Mice

Chang, Wun-Ling, Jones, Steven P., Lefer, David J., Welbourne, Tomas, Sun, Guang, Yin, Lijia, ...

The Plasmodium berghei-infected mouse model is a well-recognized model for human cerebral malaria. Mice infected with P. berghei exhibit (i) metabolic acidosis (pH < 7.3) associated with elevated...

Sexual dimorphism in reduced-size liver ischemia and reperfusion injury in mice: Role of endothelial cell nitric oxide synthase

Harada, Hirohisa, Pavlick, Kevin P., Hines, Ian N., Lefer, David J., Hoffman, Jason M., Bharwani, Sulaiman, ...

We have recently reported that female mice are protected to a much greater extent from the injurious effects of reduced-size liver ischemia and reperfusion (RSL+I/R) than are males by an...

Endothelial nitric oxide synthase overexpression attenuates congestive heart failure in mice

Jones, Steven P., Greer, James J. M., Van Haperen, Rien, Duncker, Dirk J., De Crom, Rini, Lefer, David J.

Congestive heart failure results in cardiovascular dysfunction and diminished vascular nitric oxide (NO) production. We hypothesized that overexpression of endothelial NO synthase (eNOS) within the...

The MEK1–ERK1/2 signaling pathway promotes compensated cardiac hypertrophy in transgenic mice

Bueno, Orlando F., De Windt, Leon J., Tymitz, Kevin M., Witt, Sandra A., Kimball, Thomas R., Klevitsky, Raisa, ...

Members of the mitogen-activated protein kinase (MAPK) cascade such as extracellular signal-regulated kinase (ERK), c-Jun N-terminal kinase (JNK) and p38 are implicated as important regulators of...

Cytoprotective effects of nitrite during in vivo ischemia-reperfusion of the heart and liver

Duranski, Mark R., Greer, James J.M., Dejam, Andre, Jaganmohan, Sathya, Hogg, Neil, Langston, William, ...

Nitrite represents a circulating and tissue storage form of NO whose bioactivation is mediated by the enzymatic action of xanthine oxidoreductase, nonenzymatic disproportionation, and reduction by...

Nitrite augments tolerance to ischemia/reperfusion injury via the modulation of mitochondrial electron transfer

Shiva, Sruti, Sack, Michael N., Greer, James J., Duranski, Mark, Ringwood, Lorna A., Burwell, Lindsay, ...

Nitrite (NO2−) is an intrinsic signaling molecule that is reduced to NO during ischemia and limits apoptosis and cytotoxicity at reperfusion in the mammalian heart, liver, and brain. Although the...

Hydrogen sulfide attenuates myocardial ischemia-reperfusion injury by preservation of mitochondrial function

Elrod, John W., Calvert, John W., Morrison, Joanna, Doeller, Jeannette E., Kraus, David W., Tao, Ling, ...

The recent discovery that hydrogen sulfide (H2S) is an endogenously produced gaseous second messenger capable of modulating many physiological processes, much like nitric oxide, prompted us to...

Dietary nitrite supplementation protects against myocardial ischemia-reperfusion injury

Bryan, Nathan S., Calvert, John W., Elrod, John W., Gundewar, Susheel, Ji, Sang Yong, Lefer, David J.

Nitrite has emerged as an endogenous signaling molecule with potential therapeutic implications for cardiovascular disease. Steady-state levels of nitrite are derived in part from dietary sources;...

Chronic sodium nitrite therapy augments ischemia-induced angiogenesis and arteriogenesis

Kumar, Dinesh, Branch, Billy G., Pattillo, Christopher B., Hood, Jay, Thoma, Stephen, Simpson, Stephen, ...

Chronic tissue ischemia due to defective vascular perfusion is a hallmark feature of peripheral artery disease for which minimal therapeutic options exist. We have reported that sodium nitrite...

Nitric oxide promotes distant organ protection: Evidence for an endocrine role of nitric oxide

Elrod, John W., Calvert, John W., Gundewar, Susheel, Bryan, Nathan S., Lefer, David J.

Endothelial NOS (eNOS)-derived NO has long been considered a paracrine signaling molecule only capable of affecting nearby cells because of its short half-life in blood and relatively limited...

Hydrogen sulfide attenuates hepatic ischemia-reperfusion injury: role of antioxidant and antiapoptotic signaling

Jha, Saurabh, Calvert, John W., Duranski, Mark R., Ramachandran, Arun, Lefer, David J.

Hydrogen sulfide (H2S) is an endogenously produced gaseous signaling molecule with diverse physiological activity. The potential protective effects of H2S have not been evaluated in the liver. The...