Viator, Ryan J, Rest, Richard F, Hildebrandt, Ellen, McGee, David J
Abstract Background Arginase (RocF) hydrolyzes L-arginine to L-ornithine and urea. While previously characterized arginases have an alkaline pH optimum and require activation with manganese, arginase...
Hovey, Justin G, Watson, Emily L, Langford, Melanie L, Hildebrandt, Ellen, Bathala, Sangeetha, Bolland, Jeffrey R, ...
Abstract Background Clinical isolates of the gastric pathogen Helicobacter pylori display a high level of genetic macro- and microheterogeneity, featuring a panmictic, rather than clonal structure....
In vivo behavior of a Helicobacter pylori SS1 nixA mutant with reduced urease activity (2002)
Nolan, Kylie J., McGee, David J., Mitchell, Hazel M., Kolesnikow, Tassia, Harro, Janette M., O'Rourke, Jani, ...
Helicobacter pylori mutants devoid of urease activity fail to colonize the gastric mucosa of mice; however, the effect of decreased levels of urease on colonization has not been examined. The nixA...
In vivo behavior of a Helicobacter pylori SS1 nixA mutant with reduced urease activity (2002)
Nolan, Kylie J., McGee, David J., Mitchell, Hazel M., Kolesnikow, Tassia, Harro, Janette M., O'Rourke, Jani, ...
Helicobacter pylori mutants devoid of urease activity fail to colonize the gastric mucosa of mice; however, the effect of decreased levels of urease on colonization has not been examined. The nixA...
In vivo behavior of a Helicobacter pylori SS1 nixA mutant with reduced urease activity (2002)
Nolan, Kylie J., McGee, David J., Mitchell, Hazel M., Kolesnikow, Tassia, Harro, Janette M., O'Rourke, Jani, ...
Helicobacter pylori mutants devoid of urease activity fail to colonize the gastric mucosa of mice; however, the effect of decreased levels of urease on colonization has not been examined. The nixA...
In vivo behavior of a Helicobacter pylori SS1 nixA mutant with reduced urease activity (2002)
Nolan, Kylie J., McGee, David J., Mitchell, Hazel M., Kolesnikow, Tassia, Harro, Janette M., O'Rourke, Jani, ...
Helicobacter pylori mutants devoid of urease activity fail to colonize the gastric mucosa of mice; however, the effect of decreased levels of urease on colonization has not been examined. The nixA...
In vivo behavior of a Helicobacter pylori SS1 nixA mutant with reduced urease activity (2002)
Nolan, Kylie J., McGee, David J., Mitchell, Hazel M., Kolesnikow, Tassia, Harro, Janette M., O'Rourke, Jani, ...
Helicobacter pylori mutants devoid of urease activity fail to colonize the gastric mucosa of mice; however, the effect of decreased levels of urease on colonization has not been examined. The nixA...
In vivo behavior of a Helicobacter pylori SS1 nixA mutant with reduced urease activity (2002)
Nolan, Kylie J., McGee, David J., Mitchell, Hazel M., Kolesnikow, Tassia, Harro, Janette M., O'Rourke, Jani, ...
Helicobacter pylori mutants devoid of urease activity fail to colonize the gastric mucosa of mice; however, the effect of decreased levels of urease on colonization has not been examined. The nixA...
In vivo behavior of a Helicobacter pylori SS1 nixA mutant with reduced urease activity (2002)
Nolan, Kylie J., McGee, David J., Mitchell, Hazel M., Kolesnikow, Tassia, Harro, Janette M., O'Rourke, Jani, ...
Helicobacter pylori mutants devoid of urease activity fail to colonize the gastric mucosa of mice; however, the effect of decreased levels of urease on colonization has not been examined. The nixA...
In vivo behavior of a Helicobacter pylori SS1 nixA mutant with reduced urease activity (2002)
Nolan, Kylie J., McGee, David J., Mitchell, Hazel M., Kolesnikow, Tassia, Harro, Janette M., O'Rourke, Jani, ...
Helicobacter pylori mutants devoid of urease activity fail to colonize the gastric mucosa of mice; however, the effect of decreased levels of urease on colonization has not been examined. The nixA...
In vivo behavior of a Helicobacter pylori SS1 nixA mutant with reduced urease activity (2002)
Nolan, Kylie J., McGee, David J., Mitchell, Hazel M., Kolesnikow, Tassia, Harro, Janette M., O'Rourke, Jani, ...
Helicobacter pylori mutants devoid of urease activity fail to colonize the gastric mucosa of mice; however, the effect of decreased levels of urease on colonization has not been examined. The nixA...
Gobert, Alain P., McGee, David J., Akhtar, Mahmood, Mendz, George L., Newton, Jamie C., Cheng, Yulan, ...
The antimicrobial effect of nitric oxide (NO) is an essential part of innate immunity. The vigorous host response to the human gastric pathogen Helicobacter pylori fails to eradicate the organism,...
Testerman, Traci L., McGee, David J., Mobley, Harry L. T.
Obstacles continue to hinder in vitro studies of the gastric human pathogen Helicobacter pylori, including difficulty culturing the organism in the absence of serum or blood, rapid loss of viability...
Isolation of Helicobacter pylori Genes That Modulate Urease Activity
McGee, David J., May, Carrie A., Garner, Rachel M., Himpsl, Janette M., Mobley, Harry L. T.
Helicobacter pylori urease, a nickel-requiring metalloenzyme, hydrolyzes urea to NH3 and CO2. We sought to identify H. pylori genes that modulate urease activity by constructing pHP8080, a plasmid...
Cloning, Expression, and Catalytic Activity of Helicobacter hepaticus Urease
Beckwith, Catherine S., McGee, David J., Mobley, Harry L. T., Riley, Lela K.
Helicobacter hepaticus causes disease in the liver and lower intestinal tract of mice. It is strongly urease positive, although it does not live in an acidic environment. The H. hepaticus urease gene...
McGee, David J., Radcliff, Fiona J., Mendz, George L., Ferrero, Richard L., Mobley, Harry L. T.
Arginase of the Helicobacter pylori urea cycle hydrolyzes l-arginine to l-ornithine and urea. H. pylori urease hydrolyzes urea to carbon dioxide and ammonium, which neutralizes acid. Both enzymes are...
In Vivo Behavior of a Helicobacter pylori SS1 nixA Mutant with Reduced Urease Activity
Nolan, Kylie J., McGee, David J., Mitchell, Hazel M., Kolesnikow, Tassia, Harro, Janette M., O'Rourke, Jani, ...
Helicobacter pylori mutants devoid of urease activity fail to colonize the gastric mucosa of mice; however, the effect of decreased levels of urease on colonization has not been examined. The nixA...
McGee, David J., Langford, Melanie L., Watson, Emily L., Carter, J. Elliot, Chen, Yu-Ting, Ottemann, Karen M.
Helicobacter pylori causes disease in the human stomach and in mouse and gerbil stomach models. Previous results have shown that motility is critical for H. pylori to colonize mice, gerbils, and...
Packiam, Mathanraj, Shell, Dawn M., Liu, Shi V., Liu, Yao-Bin, McGee, David J., Srivastava, Ranjana, ...
α-2,3-Sialyltransferase (Lst) is expressed on the outer membrane of Neisseria gonorrhoeae and Neisseria meningitidis and sialylates surface lipooligosaccharide (LOS), facilitating resistance to...
Testerman, Traci L., Conn, P. Brian, Mobley, Harry L. T., McGee, David J.
The growth of the gastric pathogen Helicobacter pylori in the absence of serum remains challenging, and nutritional requirements have only partially been defined, while almost nothing is known about...
Gobert, Alain P., McGee, David J., Akhtar, Mahmood, Mendz, George L., Newton, Jamie C., Cheng, Yulan, ...
The antimicrobial effect of nitric oxide (NO) is an essential part of innate immunity. The vigorous host response to the human gastric pathogen Helicobacter pylori fails to eradicate the organism,...
Testerman, Traci L., McGee, David J., Mobley, Harry L. T.
Obstacles continue to hinder in vitro studies of the gastric human pathogen Helicobacter pylori, including difficulty culturing the organism in the absence of serum or blood, rapid loss of viability...
Isolation of Helicobacter pylori Genes That Modulate Urease Activity
McGee, David J., May, Carrie A., Garner, Rachel M., Himpsl, Janette M., Mobley, Harry L. T.
Helicobacter pylori urease, a nickel-requiring metalloenzyme, hydrolyzes urea to NH3 and CO2. We sought to identify H. pylori genes that modulate urease activity by constructing pHP8080, a plasmid...
Cloning, Expression, and Catalytic Activity of Helicobacter hepaticus Urease
Beckwith, Catherine S., McGee, David J., Mobley, Harry L. T., Riley, Lela K.
Helicobacter hepaticus causes disease in the liver and lower intestinal tract of mice. It is strongly urease positive, although it does not live in an acidic environment. The H. hepaticus urease gene...
McGee, David J., Radcliff, Fiona J., Mendz, George L., Ferrero, Richard L., Mobley, Harry L. T.
Arginase of the Helicobacter pylori urea cycle hydrolyzes l-arginine to l-ornithine and urea. H. pylori urease hydrolyzes urea to carbon dioxide and ammonium, which neutralizes acid. Both enzymes are...
In Vivo Behavior of a Helicobacter pylori SS1 nixA Mutant with Reduced Urease Activity
Nolan, Kylie J., McGee, David J., Mitchell, Hazel M., Kolesnikow, Tassia, Harro, Janette M., O'Rourke, Jani, ...
Helicobacter pylori mutants devoid of urease activity fail to colonize the gastric mucosa of mice; however, the effect of decreased levels of urease on colonization has not been examined. The nixA...
McGee, David J., Langford, Melanie L., Watson, Emily L., Carter, J. Elliot, Chen, Yu-Ting, Ottemann, Karen M.
Helicobacter pylori causes disease in the human stomach and in mouse and gerbil stomach models. Previous results have shown that motility is critical for H. pylori to colonize mice, gerbils, and...
Packiam, Mathanraj, Shell, Dawn M., Liu, Shi V., Liu, Yao-Bin, McGee, David J., Srivastava, Ranjana, ...
α-2,3-Sialyltransferase (Lst) is expressed on the outer membrane of Neisseria gonorrhoeae and Neisseria meningitidis and sialylates surface lipooligosaccharide (LOS), facilitating resistance to...
Testerman, Traci L., Conn, P. Brian, Mobley, Harry L. T., McGee, David J.
The growth of the gastric pathogen Helicobacter pylori in the absence of serum remains challenging, and nutritional requirements have only partially been defined, while almost nothing is known about...
Hovey, Justin G, Watson, Emily L, Langford, Melanie L, Hildebrandt, Ellen, Bathala, Sangeetha, Bolland, Jeffrey R, ...
Williams, Susan M., Chen, Yu-Ting, Andermann, Tessa M., Carter, J. Elliot, McGee, David J., Ottemann, Karen M.
The ulcer-causing pathogen Helicobacter pylori uses directed motility, or chemotaxis, to both colonize the stomach and promote disease development. Previous work showed that mutants lacking the TlpB...