David J. Steger

PPAR{gamma} and C/EBP factors orchestrate adipocyte biology via adjacent binding on a genome-wide scale (2008)

Lefterova, Martina I., Zhang, Yong, Steger, David J., Schupp, Michael, Schug, Jonathan, Cristancho, Ana, ...

Peroxisome proliferator-activated receptor γ(PPARγ), a nuclear receptor and the target of anti-diabetic thiazolinedione drugs, is known as the master regulator of adipocyte biology. Although it...

AP-2 family members regulate basal and cAMP-induced expression of human chorionic gonadotropin (2000)

LiCalsi, Cynthia, Christophe, Sylvie, Steger, David J., Buescher, Marita, Fischer, Wolfgang, Mellon, Pamela L.

The AP-2 family of transcriptional regulator proteins has three members, α, &bgr; and &ggr;. AP-2α and &ggr; are expressed in placenta and in the human trophoblast cell line JEG-3. AP-2 has been...

Purified histone acetyltransferase complexes stimulate HIV-1 transcription from preassembled nucleosomal arrays

Steger, David J., Eberharter, Anton, John, Sam, Grant, Patrick A., Workman, Jerry L.

Protein acetylation has been implicated in the regulation of HIV-1 gene transcription. Here, we have exploited the activities of four native histone acetyltransferase (HAT) complexes from yeast to...

Activation Domain-Specific and General Transcription Stimulation by Native Histone Acetyltransferase Complexes

Ikeda, Keiko, Steger, David J., Eberharter, Anton, Workman, Jerry L.

Recent progress in identifying the catalytic subunits of histone acetyltransferase (HAT) complexes has implicated histone acetylation in the regulation of transcription. Here, we have analyzed the...

A Novel H2A/H4 Nucleosomal Histone Acetyltransferase in Tetrahymena thermophila

Ohba, Reiko, Steger, David J., Brownell, James E., Mizzen, Craig A., Cook, Richard G., Côté, Jacques, ...

Recently, we reported the identification of a 55-kDa polypeptide (p55) from Tetrahymena macronuclei as a catalytic subunit of a transcription-associated histone acetyltransferase (HAT A). Extensive...

AP-2 family members regulate basal and cAMP-induced expression of human chorionic gonadotropin

LiCalsi, Cynthia, Christophe, Sylvie, Steger, David J., Buescher, Marita, Fischer, Wolfgang, Mellon, Pamela L.

The AP-2 family of transcriptional regulator proteins has three members, α, β and γ. AP-2α and γ are expressed in placenta and in the human trophoblast cell line JEG-3. AP-2 has been shown to...

Distribution of acetylated histones resulting from Gal4-VP16 recruitment of SAGA and NuA4 complexes

Vignali, Marissa, Steger, David J., Neely, Kristen E., Workman, Jerry L.

We analyzed the targeting of histone acetyltransferase (HAT) complexes by DNA-binding activators during transcriptional activation and the resulting distribution of acetylated histones. An in vitro...

The chromo domain protein Chd1p from budding yeast is an ATP-dependent chromatin-modifying factor

Tran, Hien G., Steger, David J., Iyer, Vishwanath R., Johnson, Alexander D.

CHD proteins are members of the chromo domain family, a class of proteins involved in transcription, DNA degradation and chromatin structure. In higher eukaryotes, there are two distinct subfamilies...

Regulation of Chromatin Remodeling by Inositol Polyphosphates

Steger, David J., Haswell, Elizabeth S., Miller, Aimee L., Wente, Susan R., OòShea, Erin K.

Chromatin remodeling is required for efficient transcription of eukaryotic genes. In a genetic selection for budding yeast mutants that were defective in induction of the phosphate-responsive PHO5...

Purified histone acetyltransferase complexes stimulate HIV-1 transcription from preassembled nucleosomal arrays

Steger, David J., Eberharter, Anton, John, Sam, Grant, Patrick A., Workman, Jerry L.

Protein acetylation has been implicated in the regulation of HIV-1 gene transcription. Here, we have exploited the activities of four native histone acetyltransferase (HAT) complexes from yeast to...

Activation Domain-Specific and General Transcription Stimulation by Native Histone Acetyltransferase Complexes

Ikeda, Keiko, Steger, David J., Eberharter, Anton, Workman, Jerry L.

Recent progress in identifying the catalytic subunits of histone acetyltransferase (HAT) complexes has implicated histone acetylation in the regulation of transcription. Here, we have analyzed the...

A Novel H2A/H4 Nucleosomal Histone Acetyltransferase in Tetrahymena thermophila

Ohba, Reiko, Steger, David J., Brownell, James E., Mizzen, Craig A., Cook, Richard G., Côté, Jacques, ...

Recently, we reported the identification of a 55-kDa polypeptide (p55) from Tetrahymena macronuclei as a catalytic subunit of a transcription-associated histone acetyltransferase (HAT A). Extensive...

AP-2 family members regulate basal and cAMP-induced expression of human chorionic gonadotropin

LiCalsi, Cynthia, Christophe, Sylvie, Steger, David J., Buescher, Marita, Fischer, Wolfgang, Mellon, Pamela L.

The AP-2 family of transcriptional regulator proteins has three members, α, β and γ. AP-2α and γ are expressed in placenta and in the human trophoblast cell line JEG-3. AP-2 has been shown to...

Distribution of acetylated histones resulting from Gal4-VP16 recruitment of SAGA and NuA4 complexes

Vignali, Marissa, Steger, David J., Neely, Kristen E., Workman, Jerry L.

We analyzed the targeting of histone acetyltransferase (HAT) complexes by DNA-binding activators during transcriptional activation and the resulting distribution of acetylated histones. An in vitro...

The chromo domain protein Chd1p from budding yeast is an ATP-dependent chromatin-modifying factor

Tran, Hien G., Steger, David J., Iyer, Vishwanath R., Johnson, Alexander D.

CHD proteins are members of the chromo domain family, a class of proteins involved in transcription, DNA degradation and chromatin structure. In higher eukaryotes, there are two distinct subfamilies...

DOT1L/KMT4 Recruitment and H3K79 Methylation Are Ubiquitously Coupled with Gene Transcription in Mammalian Cells▿

Steger, David J., Lefterova, Martina I., Ying, Lei, Stonestrom, Aaron J., Schupp, Michael, Zhuo, David, ...

The histone H3 lysine 79 methyltransferase DOT1L/KMT4 can promote an oncogenic pattern of gene expression through binding with several MLL fusion partners found in acute leukemia. However, the normal...

PPARγ and C/EBP factors orchestrate adipocyte biology via adjacent binding on a genome-wide scale

Lefterova, Martina I., Zhang, Yong, Steger, David J., Schupp, Michael, Schug, Jonathan, Cristancho, Ana, ...

Peroxisome proliferator-activated receptor γ(PPARγ), a nuclear receptor and the target of anti-diabetic thiazolinedione drugs, is known as the master regulator of adipocyte biology. Although it...

Retinol saturase promotes adipogenesis and is downregulated in obesity

Schupp, Michael, Lefterova, Martina I., Janke, Jürgen, Leitner, Kirstin, Cristancho, Ana G., Mullican, Shannon E., ...

Adipocyte differentiation is controlled by many transcription factors, but few known downstream targets of these factors are necessary for adipogenesis. Here we report that retinol saturase (RetSat),...

Selective Partial Agonism of Liver X Receptor α Is Related to Differential Corepressor Recruitment

Phelan, Caroline A., Weaver, Joseph M., Steger, David J., Joshi, Shree, Maslany, Jeffrey T., Collins, Jon L., ...

Classically, activated transcription by nuclear receptors (NRs) is due to a ligand-induced switch from corepressor- to coactivator-bound states. However, coactivators and corepressors recognize...