Mechanisms of ubiquitin transfer by the anaphase-promoting complex (2009)
Matyskiela, Mary E, Rodrigo-Brenni, Monica C, Morgan, David O
Abstract The anaphase-promoting complex (APC) is a ubiquitin-protein ligase required for the completion of mitosis in all eukaryotes. Recent mechanistic studies reveal how this remarkable enzyme...
An architectural map of the anaphase-promoting complex (2006)
Thornton, Brian R., Ng, Tessie M., Matyskiela, Mary E., Carroll, Christopher W., Morgan, David O., Toczyski, David P.
The anaphase-promoting complex or cyclosome (APC) is an unusually complicated ubiquitin ligase, composed of 13 core subunits and either of two loosely associated regulatory subunits, Cdc20 and Cdh1....
Exploiting Chemical Libraries, Structure, and Genomics in the Search for Kinase Inhibitors (1998)
Kim, Sung-Hou, Meijer, Laurent, LeClerc, Sophie, Barnes, Georjana, Morgan, David O., Espinoza, F. Hernan, ...
Selective protein kinase inhibitors were developed on the basis of the unexpected binding mode of 2,6,9-trisubstituted purines to the adenosine triphosphate-binding site of the human cyclin-dependent...
Exploiting Chemical Libraries, Structure, and Genomics in the Search for Kinase Inhibitors (1998)
Lockhart, David J., Kim, Sung-Hou, Meijer, Laurent, LeClerc, Sophie, Barnes, Georjana, Morgan, David O., ...
Selective protein kinase inhibitors were developed on the basis of the unexpected binding mode of 2,6,9-trisubstituted purines to the adenosine triphosphate-binding site of the human cyclin-dependent...
Cujec, Thomas P., Okamoto, Hiroshi, Fujinaga, Koh, Meyer, Jon, Chamberlin, Holly, Morgan, David O., ...
Ran-independent nuclear import of cyclin B1–Cdc2 by importin β
Takizawa, Catherine G., Weis, Karsten, Morgan, David O.
Mitosis is triggered in vertebrate cells by the cyclin B1–Cdc2 complex. The activation of this complex at the end of G2 phase is accompanied by its translocation from the cytoplasm to the nucleus....
A Late Mitotic Regulatory Network Controlling Cyclin Destruction in Saccharomyces cerevisiae
Jaspersen, Sue L., Charles, Julia F., Tinker-Kulberg, Rachel L., Morgan, David O.
Exit from mitosis requires the inactivation of mitotic cyclin-dependent kinase–cyclin complexes, primarily by ubiquitin-dependent cyclin proteolysis. Cyclin destruction is regulated by a ubiquitin...
Cdc37 Promotes the Stability of Protein Kinases Cdc28 and Cak1
Farrell, Alison, Morgan, David O.
In the budding yeast Saccharomyces cerevisiae, Cdc37 is required for the productive formation of Cdc28-cyclin complexes. The cdc37-1 mutant arrests at Start with low levels of Cdc28 protein, which is...
Cak1 Is Required for Kin28 Phosphorylation and Activation In Vivo
Espinoza, F. Hernan, Farrell, Alison, Nourse, Jamison L., Chamberlin, Holly M., Gileadi, Opher, Morgan, David O.
Garrett, Sarah, Barton, William A., Knights, Ronald, Jin, Pei, Morgan, David O., Fisher, Robert P.
Cyclin-dependent kinase 7 (CDK7) is the catalytic subunit of the metazoan CDK-activating kinase (CAK), which activates CDKs, such as CDC2 and CDK2, through phosphorylation of a conserved threonine...
Cak1 Is Required for Kin28 Phosphorylation and Activation In Vivo
Espinoza, F. Hernán, Farrell, Alison, Nourse, Jamison L., Chamberlin, Holly M., Gileadi, Opher, Morgan, David O.
Complete activation of most cyclin-dependent protein kinases (CDKs) requires phosphorylation by the CDK-activating kinase (CAK). In the budding yeast, Saccharomyces cerevisiae, the major CAK is a...
Kaposi's Sarcoma-Associated Herpesvirus K-bZIP Protein Is Phosphorylated by Cyclin-Dependent Kinases
Polson, Andrew G., Huang, Lan, Lukac, David M., Blethrow, Justin D., Morgan, David O., Burlingame, Alma L., ...
The K8 locus in Kaposi's sarcoma-associated herpesvirus (KSHV) is syntenic with the Epstein-Barr virus (EBV) BZLF (Z) locus and expresses three alternatively spliced transcripts. The fully spliced...
Cujec, Thomas P., Okamoto, Hiroshi, Fujinaga, Koh, Meyer, Jon, Chamberlin, Holly, Morgan, David O., ...
The human immunodeficiency virus encodes the transcriptional transactivator Tat, which binds to the transactivation response (TAR) RNA stem–loop in the viral long terminal repeat (LTR) and...
Pds1 and Esp1 control both anaphase and mitotic exit in normal cells and after DNA damage
Tinker-Kulberg, Rachel L., Morgan, David O.
The separation of sister chromatids in anaphase is followed by spindle disassembly and cytokinesis. These events are governed by the anaphase-promoting complex (APC), which triggers the...
An architectural map of the anaphase-promoting complex
Thornton, Brian R., Ng, Tessie M., Matyskiela, Mary E., Carroll, Christopher W., Morgan, David O., Toczyski, David P.
The anaphase-promoting complex or cyclosome (APC) is an unusually complicated ubiquitin ligase, composed of 13 core subunits and either of two loosely associated regulatory subunits, Cdc20 and Cdh1....
Ran-independent nuclear import of cyclin B1–Cdc2 by importin β
Takizawa, Catherine G., Weis, Karsten, Morgan, David O.
Mitosis is triggered in vertebrate cells by the cyclin B1–Cdc2 complex. The activation of this complex at the end of G2 phase is accompanied by its translocation from the cytoplasm to the nucleus....
A Late Mitotic Regulatory Network Controlling Cyclin Destruction in Saccharomyces cerevisiae
Jaspersen, Sue L., Charles, Julia F., Tinker-Kulberg, Rachel L., Morgan, David O.
Exit from mitosis requires the inactivation of mitotic cyclin-dependent kinase–cyclin complexes, primarily by ubiquitin-dependent cyclin proteolysis. Cyclin destruction is regulated by a ubiquitin...
Cdc37 Promotes the Stability of Protein Kinases Cdc28 and Cak1
Farrell, Alison, Morgan, David O.
In the budding yeast Saccharomyces cerevisiae, Cdc37 is required for the productive formation of Cdc28-cyclin complexes. The cdc37-1 mutant arrests at Start with low levels of Cdc28 protein, which is...
Cak1 Is Required for Kin28 Phosphorylation and Activation In Vivo
Espinoza, F. Hernan, Farrell, Alison, Nourse, Jamison L., Chamberlin, Holly M., Gileadi, Opher, Morgan, David O.
Garrett, Sarah, Barton, William A., Knights, Ronald, Jin, Pei, Morgan, David O., Fisher, Robert P.
Cyclin-dependent kinase 7 (CDK7) is the catalytic subunit of the metazoan CDK-activating kinase (CAK), which activates CDKs, such as CDC2 and CDK2, through phosphorylation of a conserved threonine...
Cak1 Is Required for Kin28 Phosphorylation and Activation In Vivo
Espinoza, F. Hernán, Farrell, Alison, Nourse, Jamison L., Chamberlin, Holly M., Gileadi, Opher, Morgan, David O.
Complete activation of most cyclin-dependent protein kinases (CDKs) requires phosphorylation by the CDK-activating kinase (CAK). In the budding yeast, Saccharomyces cerevisiae, the major CAK is a...
Kaposi's Sarcoma-Associated Herpesvirus K-bZIP Protein Is Phosphorylated by Cyclin-Dependent Kinases
Polson, Andrew G., Huang, Lan, Lukac, David M., Blethrow, Justin D., Morgan, David O., Burlingame, Alma L., ...
The K8 locus in Kaposi's sarcoma-associated herpesvirus (KSHV) is syntenic with the Epstein-Barr virus (EBV) BZLF (Z) locus and expresses three alternatively spliced transcripts. The fully spliced...
Cujec, Thomas P., Okamoto, Hiroshi, Fujinaga, Koh, Meyer, Jon, Chamberlin, Holly, Morgan, David O., ...
The human immunodeficiency virus encodes the transcriptional transactivator Tat, which binds to the transactivation response (TAR) RNA stem–loop in the viral long terminal repeat (LTR) and...
Pds1 and Esp1 control both anaphase and mitotic exit in normal cells and after DNA damage
Tinker-Kulberg, Rachel L., Morgan, David O.
The separation of sister chromatids in anaphase is followed by spindle disassembly and cytokinesis. These events are governed by the anaphase-promoting complex (APC), which triggers the...
An architectural map of the anaphase-promoting complex
Thornton, Brian R., Ng, Tessie M., Matyskiela, Mary E., Carroll, Christopher W., Morgan, David O., Toczyski, David P.
The anaphase-promoting complex or cyclosome (APC) is an unusually complicated ubiquitin ligase, composed of 13 core subunits and either of two loosely associated regulatory subunits, Cdc20 and Cdh1....
Ko, Nolan, Nishihama, Ryuichi, Tully, Gregory H., Ostapenko, Denis, Solomon, Mark J., Morgan, David O., ...
In the yeast Saccharomyces cerevisiae, a ring of myosin II forms in a septin-dependent manner at the budding site in late G1. This ring remains at the bud neck until the onset of cytokinesis, when...
Nuclear Localization of Cyclin B1 Controls Mitotic Entry After DNA Damage
Jin, Pei, Hardy, Stephen, Morgan, David O.
Mitosis in human cells is initiated by the protein kinase Cdc2-cyclin B1, which is activated at the end of G2 by dephosphorylation of two inhibitory residues, Thr14 and Tyr15. The G2 arrest that...
Covalent capture of kinase-specific phosphopeptides reveals Cdk1-cyclin B substrates
Blethrow, Justin D., Glavy, Joseph S., Morgan, David O., Shokat, Kevan M.
We describe a method for rapid identification of protein kinase substrates. Cdk1 was engineered to accept an ATP analog that allows it to uniquely label its substrates with a bio-orthogonal phosphate...
Cyclin-Specific Control of Ribosomal DNA Segregation▿
Sullivan, Matt, Holt, Liam, Morgan, David O.
Following chromosome duplication in S phase of the cell cycle, the sister chromatids are linked by cohesin. At the onset of anaphase, separase cleaves cohesin and thereby initiates sister chromatid...
The Anaphase-promoting Complex Promotes Actomyosin-Ring Disassembly during Cytokinesis in Yeast
Tully, Gregory H., Nishihama, Ryuichi, Pringle, John R., Morgan, David O.
The anaphase-promoting complex (APC) is a ubiquitin ligase that controls progression through mitosis by targeting specific proteins for degradation. It is unclear whether the APC also contributes to...