A single Hox locus in Drosophila produces functional microRNAs from opposite DNA strands (2010)
Er Stark, Natascha Bushati, Calvin Jan, Pouya Kheradpour, Julius Brennecke, Ingrid Ibarra, ...
MicroRNAs (miRNAs) are ~22-nucleotide RNAs that are transcribed as longer precursors and processed from characteristic pre-miRNA hairpins. They pair to sites in protein-coding messages to direct...
Mammalian microRNAs: experimental evaluation of novel and previously annotated genes (2010)
Chiang, H. Rosaria, Schoenfeld, Lori W., Ruby, J. Graham, Auyeung, Vincent C., Spies, Noah, Baek, Daehyun, ...
MicroRNAs (miRNAs) are small regulatory RNAs that derive from distinctive hairpin transcripts. To learn more about the miRNAs of mammals, we sequenced 60 million small RNAs from mouse brain, ovary,...
Andrew Grimson, Wendy K. Johnston, Philip Garrett-engele, Lee P. Lim, David P. Bartel
Mammalian microRNAs (miRNAs) pair to 3 0 UTRs of mRNAs to direct their posttranscriptional repression. Important for target recognition are 7 nt sites that match the seed region of the miRNA....
Most mammalian mRNAs are conserved targets of microRNAs (2009)
Friedman, Robin C., Farh, Kyle Kai-How, Burge, Christopher B., Bartel, David P.
MicroRNAs (miRNAs) are small endogenous RNAs that pair to sites in mRNAs to direct post-transcriptional repression. Many sites that match the miRNA seed (nucleotides 2–7), particularly those in...
Shkumatava, Alena, Stark, Alexander, Sive, Hazel, Bartel, David P.
MicroRNAs (miRNAs) are small noncoding RNAs that direct post-transcriptional repression of protein-coding genes. In vertebrates, each highly conserved miRNA typically regulates hundreds of target...
Bagby, Sarah C., Bergman, Nicholas H., Shechner, David M., Yen, Catherine, Bartel, David P.
The class I ligase was among the first ribozymes to have been isolated from random sequences and represents the catalytic core of several RNA-directed RNA polymerase ribozymes. The ligase is also...
1540 BREVIA Vertebrate MicroRNA Genes (2008)
Lee P. Lim, Margaret E. Glasner, Soraya Yekta, Christopher B. Burge, David P. Bartel
MicroRNAs (miRNAs) are an abundant class of �22-nucleotide (nt) noncoding RNAs, some of which are known to control the expression of other genes at the posttranscriptional level (1–4). We...
PRG-1 and 21U-RNAs interact to form the piRNA complex required for fertility in C. elegans (2008)
Batista, Pedro J., Ruby, J. Graham, Claycomb, Julie M., Chiang, H. Rosaria, Fahlgren, Noah, Kasschau, Kristin D., ...
In metazoans, Piwi-related Argonaute proteins have been linked to germline maintenance, and to a class of germline-enriched small RNAs termed piRNAs. Here we show that an abundant class of 21...
Babiarz, Joshua E., Ruby, J. Graham, Wang, Yangming, Bartel, David P., Blelloch, Robert
Canonical microRNAs (miRNAs) require two processing steps: the first by the Microprocessor, a complex of DGCR8 and Drosha, and the second by a complex of TRBP and Dicer. dgcr8Δ/Δ mouse embryonic...
A single Hox locus in Drosophila produces functional microRNAs from opposite DNA strands (2008)
Stark, Alexander, Bushati, Natascha, Jan, Calvin H., Kheradpour, Pouya, Hodges, Emily, Brennecke, Julius, ...
MicroRNAs (miRNAs) are ∼22-nucleotide RNAs that are processed from characteristic precursor hairpins and pair to sites in messages of protein-coding genes to direct post-transcriptional repression....
Improved polymerase ribozyme efficiency on hydrophobic assemblies (2008)
Müller, Ulrich F., Bartel, David P.
During an early step in the evolution of life, RNA served both as genome and as catalyst, according to the RNA world hypothesis. For self-replication, the RNA organisms must have contained an RNA...
Improved polymerase ribozyme efficiency on hydrophobic assemblies (2008)
Müller, Ulrich F., Bartel, David P.
During an early step in the evolution of life, RNA served both as genome and as catalyst, according to the RNA world hypothesis. For self-replication, the RNA organisms must have contained an RNA...
Most mammalian mRNAs are conserved targets of microRNAs (2008)
Friedman, Robin C., Farh, Kyle Kai-How, Burge, Christopher B., Bartel, David P.
MicroRNAs (miRNAs) are small endogenous RNAs that pair to sites in mRNAs to direct post-transcriptional repression. Many sites that match the miRNA seed (nucleotides 2–7), particularly those in...
Improved polymerase ribozyme efficiency on hydrophobic assemblies (2008)
Müller, Ulrich F., Bartel, David P.
During an early step in the evolution of life, RNA served both as genome and as catalyst, according to the RNA world hypothesis. For self-replication, the RNA organisms must have contained an RNA...
Most mammalian mRNAs are conserved targets of microRNAs (2008)
Friedman, Robin C., Farh, Kyle Kai-How, Burge, Christopher B., Bartel, David P.
MicroRNAs (miRNAs) are small endogenous RNAs that pair to sites in mRNAs to direct post-transcriptional repression. Many sites that match the miRNA seed (nucleotides 2–7), particularly those in...
Eric A. Miska, Ezequiel Alvarez-Saavedra, Allison L. Abbott, Nelson C. Lau, Andrew B. Hellman, Shannon M. McGonagle, ...
MicroRNAs (miRNAs), a large class of short noncoding RNAs found in many plants and animals, often act to post-transcriptionally inhibit gene expression. We report the generation of deletion mutations...
Discovery of functional elements in 12 Drosophila genomes using evolutionary signatures (2007)
Alexander Stark, Michael F. Lin, Pouya Kheradpour, Jakob S. Pedersen, Leopold Parts, Joseph W. Carlson, ...
Discovery of functional elements in 12 Drosophila genomes using evolutionary signatures (2007)
Lin, Michael F, Kheradpour, Pouya, Pedersen, Jakob S, Parts, Leopold, Carlson, Joseph W, ...
Sequencing of multiple related species followed by comparative genomics analysis constitutes a powerful approach for the systematic understanding of any genome. Here, we use the genomes of 12...
Ruby, J. Graham, Stark, Alexander, Johnston, Wendy K., Kellis, Manolis, Bartel, David P., Lai, Eric C.
MicroRNA (miRNA) genes give rise to small regulatory RNAs in a wide variety of organisms. We used computational methods to predict miRNAs conserved among Drosophila species and large-scale sequencing...
A diverse and evolutionarily fluid set of microRNAs in Arabidopsis thaliana (2006)
Rajagopalan, Ramya, Vaucheret, Hervé, Trejo, Jerry, Bartel, David P.
To better understand the diversity of small silencing RNAs expressed in plants, we employed high-throughput pyrosequencing to obtain 887,000 reads corresponding to Arabidopsis thaliana small RNAs....
The microRNA miR-196 acts upstream of Hoxb8 and Shh in limb development (2005)
Eran Hornstein, Jennifer H. Mansfield, Soraya Yekta, Brian D. Harfe, Michael T. McManus, ...
Matranga, Christian B., Tomari, Yukihide, Shin, Chanseok, Bartel, David P., Zamore, Phillip D.
In the Drosophila and mammalian RNA interference pathways, siRNAs direct the protein Argonaute2 (Ago2) to cleave corresponding mRNA targets, silencing their expression. Ago2 is the catalytic...
Microarray analysis shows that some microRNAs downregulate large numbers of target mRNAs (2005)
Lee P. Lim, Nelson C. Lau, Philip Garrett-Engele, Andrew Grimson, Janell M. Schelter, John Castle, ...
BASKERVILLE, SCOTT, BARTEL, DAVID P.
MicroRNAs (miRNAs) are short endogenous RNAs known to post-transcriptionally repress gene expression in animals and plants. A microarray profiling survey revealed the expression patterns of 175 human...
New ligase-derived RNA polymerase ribozymes (2005)
LAWRENCE, MICHAEL S., BARTEL, DAVID P.
The search is underway for a catalytic RNA molecule capable of self-replication. Finding such a ribozyme would lend crucial support to the RNA World hypothesis, which holds that very early life-forms...
New ligase-derived RNA polymerase ribozymes (2005)
LAWRENCE, MICHAEL S., BARTEL, DAVID P.
The search is underway for a catalytic RNA molecule capable of self-replication. Finding such a ribozyme would lend crucial support to the RNA World hypothesis, which holds that very early life-forms...
New ligase-derived RNA polymerase ribozymes (2005)
LAWRENCE, MICHAEL S., BARTEL, DAVID P.
The search is underway for a catalytic RNA molecule capable of self-replication. Finding such a ribozyme would lend crucial support to the RNA World hypothesis, which holds that very early life-forms...
MicroRNAs Modulate Hematopoietic Lineage Differentiation (2004)
Lodish, Harvey F., Chen, Chang-Zheng, Bartel, David P.
MicroRNAs (miRNAs), an abundant class of ~22 nucleotide non-coding RNAs, are thought to play an important regulatory role in animal and plant development at the posttranscriptional level. Many miRNAs...
MicroRNAs Modulate Hematopoietic Lineage Differentiation (2004)
Lodish, Harvey F., Chen, Chang-Zheng, Bartel, David P.
MicroRNAs (miRNAs), an abundant class of ~22 nucleotide non-coding RNAs, are thought to play an important regulatory role in animal and plant development at the posttranscriptional level. Many miRNAs...
Vaucheret, Hervé, Vazquez, Franck, Crété, Patrice, Bartel, David P.
MicroRNAs (miRNAs) are endogenous 21–24-nt RNAs that can down-regulate gene expression by pairing to the messages of protein-coding genes to specify mRNA cleavage or repression of productive...
Vaucheret, Hervá, Vazquez, Franck, Crátá, Patrice, Bartel, David P.
MicroRNAs (miRNAs) are endogenous 21-24-nt RNAs that can down-regulate gene expression by pairing to the messages of protein-coding genes to specify mRNA cleavage or repression of productive...
The three-dimensional architecture of the class I ligase ribozyme (2004)
BERGMAN, NICHOLAS H., LAU, NELSON C., LEHNERT, VALERIE, WESTHOF, ERIC, BARTEL, DAVID P.
The class I ligase ribozyme catalyzes a Mg++-dependent RNA-ligation reaction that is chemically analogous to a single step of RNA polymerization. Indeed, this ribozyme constitutes the catalytic...
OHLER, UWE, YEKTA, SORAYA, LIM, LEE P., BARTEL, DAVID P., BURGE, CHRISTOPHER B.
MicroRNAs are ~22-nucleotide (nt) RNAs processed from foldback segments of endogenous transcripts. Some are known to play important gene regulatory roles during animal and plant development by...
Vaucheret, Hervá, Vazquez, Franck, Crátá, Patrice, Bartel, David P.
MicroRNAs (miRNAs) are endogenous 21-24-nt RNAs that can down-regulate gene expression by pairing to the messages of protein-coding genes to specify mRNA cleavage or repression of productive...
A biochemical framework for RNA silencing in plants (2003)
Tang, Guiliang, Reinhart, Brenda J., Bartel, David P., Zamore, Phillip D.
RNA silencing phenomena were first discovered in plants, yet only the RNA interference pathway in animals has been subject to biochemical analysis. Here, we extend biochemical analysis to plant RNA...
The microRNAs of Caenorhabditis elegans (2003)
Lim, Lee P., Lau, Nelson C., Weinstein, Earl G., Abdelhakim, Aliaa, Yekta, Soraya, Rhoades, Matthew W., ...
A biochemical framework for RNA silencing in plants (2003)
Tang, Guiliang, Reinhart, Brenda J., Bartel, David P., Zamore, Phillip D.
A uniform system for microRNA annotation (2003)
AMBROS, VICTOR, BARTEL, BONNIE, BARTEL, DAVID P., BURGE, CHRISTOPHER B., CARRINGTON, JAMES C., CHEN, XUEMEI, ...
MicroRNAs (miRNAs) are small noncoding RNA gene products about 22 nt long that are processed by Dicer from precursors with a characteristic hairpin secondary structure. Guidelines are presented for...
CHAPPLE, KELLY E., BARTEL, DAVID P., UNRAU, PETER J.
We previously isolated from random sequences ribozymes able to form a glycosidic linkage between a ribose sugar and 4-thiouracil in a reaction that mimics protein-catalyzed nucleotide synthesis. Here...
The microRNAs of Caenorhabditis elegans (2003)
Lim, Lee P., Lau, Nelson C., Weinstein, Earl G., Abdelhakim, Aliaa, Yekta, Soraya, Rhoades, Matthew W., ...
The microRNAs of Caenorhabditis elegans (2003)
Lim, Lee P., Lau, Nelson C., Weinstein, Earl G., Abdelhakim, Aliaa, Yekta, Soraya, Rhoades, Matthew W., ...
2001, `RNA-Catalyzed RNA Polymerization: Accurate and General RNA-Templated Primer Extension (2001)
Wendy K. Johnston, Peter J. Unrau, Michael S. Lawrence, Margaret E. Glasner, David P. Bartel
The RNA world hypothesis regarding the early evolution of life relies on the premise that some RNA sequences can catalyze RNA replication. In support of this conjecture, we describe here an RNA...
Kelly P. Williams, David P. Bartel
tmRNA (also known as 10Sa RNA) is so-named for its dual tRNA-like and mRNA-like nature. It is employed in a remarkable trans-translation process to add a Cterminal peptide tag to the incomplete...
Targeted mRNA degradation by double-stranded RNA in vitro (1999)
Tuschl, Thomas, Zamore, Phillip D., Lehmann, Ruth, Bartel, David P., Sharp, Phillip A.
Kelly P. Williams, David P. Bartel
tmRNA (also known as 10Sa RNA) is so-named for its dual tRNA-like and mRNA-like nature. It is employed in a remarkable trans-translation process to add a Cterminal peptide tag to the incomplete...
The secondary structure and sequence optimization of an RNA ligase ribozyme (1995)
Ekland, Eric H., Bartel, David P.
In vitro selection can generate functional sequence variants of an RNA structural motif that are useful for comparative analysis. The technique Is particularly valuable in cases where natural...
Reverse transcriptase reads through a 2'-5' linkage and a 2'-thiphosphate in a template (1995)
Lorsch, John R., Bartel, David P., Szostak, Jack W.
Avian myeloblastosis virus and Maloney murlne leukemia virus RNase H− reverse transcriptases pause when they encounter a 2′–5′ linkage or a 2′-thiophosphate In their template RNAs, but...
Thesis (Ph. D.)--Harvard University, 1993.
Bioactive and nuclease-resistant l-DNA ligand of vasopressin
Williams, Kelly P., Liu, Xiao-Hong, Schumacher, Ton N. M., Lin, Herbert Y., Ausiello, Dennis A., Kim, Peter S., ...
In vitro selection experiments have produced nucleic acid ligands (aptamers) that bind tightly and specifically to a great variety of target biomolecules. The utility of aptamers is often limited by...
A ribozyme that ligates RNA to protein
Baskerville, Scott, Bartel, David P.
We have used a combination of in vitro selection and rational design to generate ribozymes that form a stable phosphoamide bond between the 5′ terminus of an RNA and a specific polypeptide. This...
Reinhart, Brenda J., Weinstein, Earl G., Rhoades, Matthew W., Bartel, Bonnie, Bartel, David P.
MicroRNAs (miRNAs) are an extensive class of ∼22-nucleotide noncoding RNAs thought to regulate gene expression in metazoans. We find that miRNAs are also present in plants, indicating that this...
A biochemical framework for RNA silencing in plants
Tang, Guiliang, Reinhart, Brenda J., Bartel, David P., Zamore, Phillip D.
RNA silencing phenomena were first discovered in plants, yet only the RNA interference pathway in animals has been subject to biochemical analysis. Here, we extend biochemical analysis to plant RNA...
The microRNAs of Caenorhabditis elegans
Lim, Lee P., Lau, Nelson C., Weinstein, Earl G., Abdelhakim, Aliaa, Yekta, Soraya, Rhoades, Matthew W., ...
MicroRNAs (miRNAs) are an abundant class of tiny RNAs thought to regulate the expression of protein-coding genes in plants and animals. In the present study, we describe a computational procedure to...
An oxocarbenium-ion intermediate of a ribozyme reaction indicated by kinetic isotope effects
Unrau, Peter J., Bartel, David P.
Many of the enzymes that catalyze reactions at nucleotide glycosidic linkages proceed through either a reactive oxocarbenium-ion intermediate or a transition state with considerable oxocarbenium...
Targeted mRNA degradation by double-stranded RNA in vitro
Tuschl, Thomas, Zamore, Phillip D., Lehmann, Ruth, Bartel, David P., Sharp, Phillip A.
Double-stranded RNA (dsRNA) directs gene-specific, post-transcriptional silencing in many organisms, including vertebrates, and has provided a new tool for studying gene function. The biochemical...
Vaucheret, Hervé, Vazquez, Franck, Crété, Patrice, Bartel, David P.
MicroRNAs (miRNAs) are endogenous 21–24-nt RNAs that can down-regulate gene expression by pairing to the messages of protein-coding genes to specify mRNA cleavage or repression of productive...
MicroRNA control of PHABULOSA in leaf development: importance of pairing to the microRNA 5′ region
Mallory, Allison C, Reinhart, Brenda J, Jones-Rhoades, Matthew W, Tang, Guiliang, Zamore, Phillip D, Barton, M Kathryn, ...
MicroRNAs (miRNAs) are ∼22-nucleotide noncoding RNAs that can regulate gene expression by directing mRNA degradation or inhibiting productive translation. Dominant mutations in PHABULOSA (PHB) and...
A uniform system for microRNA annotation
AMBROS, VICTOR, BARTEL, BONNIE, BARTEL, DAVID P., BURGE, CHRISTOPHER B., CARRINGTON, JAMES C., CHEN, XUEMEI, ...
MicroRNAs (miRNAs) are small noncoding RNA gene products about 22 nt long that are processed by Dicer from precursors with a characteristic hairpin secondary structure. Guidelines are presented for...
Combinatorial minimization and secondary structure determination of a nucleotide synthase ribozyme
CHAPPLE, KELLY E., BARTEL, DAVID P., UNRAU, PETER J.
We previously isolated from random sequences ribozymes able to form a glycosidic linkage between a ribose sugar and 4-thiouracil in a reaction that mimics protein-catalyzed nucleotide synthesis. Here...
The three-dimensional architecture of the class I ligase ribozyme
BERGMAN, NICHOLAS H., LAU, NELSON C., LEHNERT, VALERIE, WESTHOF, ERIC, BARTEL, DAVID P.
The class I ligase ribozyme catalyzes a Mg++-dependent RNA-ligation reaction that is chemically analogous to a single step of RNA polymerization. Indeed, this ribozyme constitutes the catalytic...
OHLER, UWE, YEKTA, SORAYA, LIM, LEE P., BARTEL, DAVID P., BURGE, CHRISTOPHER B.
MicroRNAs are ~22-nucleotide (nt) RNAs processed from foldback segments of endogenous transcripts. Some are known to play important gene regulatory roles during animal and plant development by...
BASKERVILLE, SCOTT, BARTEL, DAVID P.
MicroRNAs (miRNAs) are short endogenous RNAs known to post-transcriptionally repress gene expression in animals and plants. A microarray profiling survey revealed the expression patterns of 175 human...
New ligase-derived RNA polymerase ribozymes
LAWRENCE, MICHAEL S., BARTEL, DAVID P.
The search is underway for a catalytic RNA molecule capable of self-replication. Finding such a ribozyme would lend crucial support to the RNA World hypothesis, which holds that very early life-forms...
Mallory, Allison C., Bartel, David P., Bartel, Bonnie
The phytohormone auxin plays critical roles during plant growth, many of which are mediated by the auxin response transcription factor (ARF) family. MicroRNAs (miRNAs), endogenous 21-nucleotide...
Antiquity of MicroRNAs and Their Targets in Land PlantsW⃞
Axtell, Michael J., Bartel, David P.
MicroRNAs (miRNAs) affect the morphology of flowering plants by the posttranscriptional regulation of genes involved in critical developmental events. Understanding the spatial and temporal dynamics...
Bioactive and nuclease-resistant l-DNA ligand of vasopressin
Williams, Kelly P., Liu, Xiao-Hong, Schumacher, Ton N. M., Lin, Herbert Y., Ausiello, Dennis A., Kim, Peter S., ...
In vitro selection experiments have produced nucleic acid ligands (aptamers) that bind tightly and specifically to a great variety of target biomolecules. The utility of aptamers is often limited by...
A ribozyme that ligates RNA to protein
Baskerville, Scott, Bartel, David P.
We have used a combination of in vitro selection and rational design to generate ribozymes that form a stable phosphoamide bond between the 5′ terminus of an RNA and a specific polypeptide. This...
Reinhart, Brenda J., Weinstein, Earl G., Rhoades, Matthew W., Bartel, Bonnie, Bartel, David P.
MicroRNAs (miRNAs) are an extensive class of ∼22-nucleotide noncoding RNAs thought to regulate gene expression in metazoans. We find that miRNAs are also present in plants, indicating that this...
A biochemical framework for RNA silencing in plants
Tang, Guiliang, Reinhart, Brenda J., Bartel, David P., Zamore, Phillip D.
RNA silencing phenomena were first discovered in plants, yet only the RNA interference pathway in animals has been subject to biochemical analysis. Here, we extend biochemical analysis to plant RNA...
The microRNAs of Caenorhabditis elegans
Lim, Lee P., Lau, Nelson C., Weinstein, Earl G., Abdelhakim, Aliaa, Yekta, Soraya, Rhoades, Matthew W., ...
MicroRNAs (miRNAs) are an abundant class of tiny RNAs thought to regulate the expression of protein-coding genes in plants and animals. In the present study, we describe a computational procedure to...
An oxocarbenium-ion intermediate of a ribozyme reaction indicated by kinetic isotope effects
Unrau, Peter J., Bartel, David P.
Many of the enzymes that catalyze reactions at nucleotide glycosidic linkages proceed through either a reactive oxocarbenium-ion intermediate or a transition state with considerable oxocarbenium...
Targeted mRNA degradation by double-stranded RNA in vitro
Tuschl, Thomas, Zamore, Phillip D., Lehmann, Ruth, Bartel, David P., Sharp, Phillip A.
Double-stranded RNA (dsRNA) directs gene-specific, post-transcriptional silencing in many organisms, including vertebrates, and has provided a new tool for studying gene function. The biochemical...
Vaucheret, Hervé, Vazquez, Franck, Crété, Patrice, Bartel, David P.
MicroRNAs (miRNAs) are endogenous 21–24-nt RNAs that can down-regulate gene expression by pairing to the messages of protein-coding genes to specify mRNA cleavage or repression of productive...
MicroRNA control of PHABULOSA in leaf development: importance of pairing to the microRNA 5′ region
Mallory, Allison C, Reinhart, Brenda J, Jones-Rhoades, Matthew W, Tang, Guiliang, Zamore, Phillip D, Barton, M Kathryn, ...
MicroRNAs (miRNAs) are ∼22-nucleotide noncoding RNAs that can regulate gene expression by directing mRNA degradation or inhibiting productive translation. Dominant mutations in PHABULOSA (PHB) and...
Mallory, Allison C., Bartel, David P., Bartel, Bonnie
The phytohormone auxin plays critical roles during plant growth, many of which are mediated by the auxin response transcription factor (ARF) family. MicroRNAs (miRNAs), endogenous 21-nucleotide...
Antiquity of MicroRNAs and Their Targets in Land PlantsW⃞
Axtell, Michael J., Bartel, David P.
MicroRNAs (miRNAs) affect the morphology of flowering plants by the posttranscriptional regulation of genes involved in critical developmental events. Understanding the spatial and temporal dynamics...
A uniform system for microRNA annotation
AMBROS, VICTOR, BARTEL, BONNIE, BARTEL, DAVID P., BURGE, CHRISTOPHER B., CARRINGTON, JAMES C., CHEN, XUEMEI, ...
MicroRNAs (miRNAs) are small noncoding RNA gene products about 22 nt long that are processed by Dicer from precursors with a characteristic hairpin secondary structure. Guidelines are presented for...
Combinatorial minimization and secondary structure determination of a nucleotide synthase ribozyme
CHAPPLE, KELLY E., BARTEL, DAVID P., UNRAU, PETER J.
We previously isolated from random sequences ribozymes able to form a glycosidic linkage between a ribose sugar and 4-thiouracil in a reaction that mimics protein-catalyzed nucleotide synthesis. Here...
The three-dimensional architecture of the class I ligase ribozyme
BERGMAN, NICHOLAS H., LAU, NELSON C., LEHNERT, VALERIE, WESTHOF, ERIC, BARTEL, DAVID P.
The class I ligase ribozyme catalyzes a Mg++-dependent RNA-ligation reaction that is chemically analogous to a single step of RNA polymerization. Indeed, this ribozyme constitutes the catalytic...
OHLER, UWE, YEKTA, SORAYA, LIM, LEE P., BARTEL, DAVID P., BURGE, CHRISTOPHER B.
MicroRNAs are ~22-nucleotide (nt) RNAs processed from foldback segments of endogenous transcripts. Some are known to play important gene regulatory roles during animal and plant development by...
BASKERVILLE, SCOTT, BARTEL, DAVID P.
MicroRNAs (miRNAs) are short endogenous RNAs known to post-transcriptionally repress gene expression in animals and plants. A microarray profiling survey revealed the expression patterns of 175 human...
New ligase-derived RNA polymerase ribozymes
LAWRENCE, MICHAEL S., BARTEL, DAVID P.
The search is underway for a catalytic RNA molecule capable of self-replication. Finding such a ribozyme would lend crucial support to the RNA World hypothesis, which holds that very early life-forms...
A diverse and evolutionarily fluid set of microRNAs in Arabidopsis thaliana
Rajagopalan, Ramya, Vaucheret, Hervé, Trejo, Jerry, Bartel, David P.
To better understand the diversity of small silencing RNAs expressed in plants, we employed high-throughput pyrosequencing to obtain 887,000 reads corresponding to Arabidopsis thaliana small RNAs....
Zhou, Beiyan, Wang, Stephanie, Mayr, Christine, Bartel, David P., Lodish, Harvey F.
MicroRNAs (miRNAs) are a family of ≈22-nt noncoding RNAs that can posttranscriptionally regulate gene expression. Several miRNAs are specifically expressed in hematopoietic cells. Here we show that...
Most Caenorhabditis elegans microRNAs Are Individually Not Essential for Development or Viability
Miska, Eric A, Alvarez-Saavedra, Ezequiel, Abbott, Allison L, Lau, Nelson C, Hellman, Andrew B, McGonagle, Shannon M, ...
MicroRNAs (miRNAs), a large class of short noncoding RNAs found in many plants and animals, often act to post-transcriptionally inhibit gene expression. We report the generation of deletion mutations...
Common Functions for Diverse Small RNAs of Land Plants[W][OA]
Axtell, Michael J., Snyder, Jo Ann, Bartel, David P.
Endogenous small RNAs, including microRNAs (miRNAs) and short interfering RNAs (siRNAs), are critical components of plant gene regulation. Some abundant miRNAs involved in developmental control are...
Ruby, J. Graham, Stark, Alexander, Johnston, Wendy K., Kellis, Manolis, Bartel, David P., Lai, Eric C.
MicroRNA (miRNA) genes give rise to small regulatory RNAs in a wide variety of organisms. We used computational methods to predict miRNAs conserved among Drosophila species and large-scale sequencing...
A single Hox locus in Drosophila produces functional microRNAs from opposite DNA strands
Stark, Alexander, Bushati, Natascha, Jan, Calvin H., Kheradpour, Pouya, Hodges, Emily, Brennecke, Julius, ...
MicroRNAs (miRNAs) are ∼22-nucleotide RNAs that are processed from characteristic precursor hairpins and pair to sites in messages of protein-coding genes to direct post-transcriptional repression....
Improved polymerase ribozyme efficiency on hydrophobic assemblies
Müller, Ulrich F., Bartel, David P.
During an early step in the evolution of life, RNA served both as genome and as catalyst, according to the RNA world hypothesis. For self-replication, the RNA organisms must have contained an RNA...
Babiarz, Joshua E., Ruby, J. Graham, Wang, Yangming, Bartel, David P., Blelloch, Robert
Canonical microRNAs (miRNAs) require two processing steps: the first by the Microprocessor, a complex of DGCR8 and Drosha, and the second by a complex of TRBP and Dicer. dgcr8Δ/Δ mouse embryonic...
Most mammalian mRNAs are conserved targets of microRNAs
Friedman, Robin C., Farh, Kyle Kai-How, Burge, Christopher B., Bartel, David P.
MicroRNAs (miRNAs) are small endogenous RNAs that pair to sites in mRNAs to direct post-transcriptional repression. Many sites that match the miRNA seed (nucleotides 2–7), particularly those in...
Coherent but overlapping expression of microRNAs and their targets during vertebrate development
Shkumatava, Alena, Stark, Alexander, Sive, Hazel, Bartel, David P.
MicroRNAs (miRNAs) are small noncoding RNAs that direct post-transcriptional repression of protein-coding genes. In vertebrates, each highly conserved miRNA typically regulates hundreds of target...
Criteria for Annotation of Plant MicroRNAs
Meyers, Blake C., Axtell, Michael J., Bartel, Bonnie, Bartel, David P., Baulcombe, David, Bowman, John L., ...
MicroRNAs (miRNAs) are ∼21 nucleotide noncoding RNAs produced by Dicer-catalyzed excision from stem-loop precursors. Many plant miRNAs play critical roles in development, nutrient homeostasis,...
McGlinn, Edwina, Yekta, Soraya, Mansfield, Jennifer H., Soutschek, Jürgen, Bartel, David P., Tabin, Clifford J.
Patterning of the vertebrate axial skeleton requires precise spatial and temporal control of Hox gene expression during embryonic development. MicroRNAs (miRNAs) are recently described modulators of...
Bagby, Sarah C., Bergman, Nicholas H., Shechner, David M., Yen, Catherine, Bartel, David P.
The class I ligase was among the first ribozymes to have been isolated from random sequences and represents the catalytic core of several RNA-directed RNA polymerase ribozymes. The ligase is also...