David W. Borhani

Rational mutagenesis to support structure-based drug design: MAPKAP kinase 2 as a case study (2009)

Argiriadi, Maria A, Sousa, Silvino, Banach, David, Marcotte, Douglas, Xiang, Tao, Tomlinson, Medha J, ...

Abstract Background Structure-based drug design (SBDD) can provide valuable guidance to drug discovery programs. Robust construct design and expression, protein purification and characterization,...

Crystal structure of truncated human apolipoprotein A-I suggests a lipid-bound conformation

Borhani, David W., Rogers, Danise P., Engler, Jeffrey A., Brouillette, Christie G.

The structure of truncated human apolipoprotein A-I (apo A-I), the major protein component of high density lipoprotein, has been determined at 4-Å resolution. The crystals comprise residues...

Slow Polymerization of Mycobacterium tuberculosis FtsZ

White, E. Lucile, Ross, Larry J., Reynolds, Robert C., Seitz, Lainne E., Moore, Georgia D., Borhani, David W.

The essential cell division protein, FtsZ, from Mycobacterium tuberculosis has been expressed in Escherichia coli and purified. The recombinant protein has GTPase activity typical of tubulin and...

Crystal structure of truncated human apolipoprotein A-I suggests a lipid-bound conformation

Borhani, David W., Rogers, Danise P., Engler, Jeffrey A., Brouillette, Christie G.

The structure of truncated human apolipoprotein A-I (apo A-I), the major protein component of high density lipoprotein, has been determined at 4-Å resolution. The crystals comprise residues...

Slow Polymerization of Mycobacterium tuberculosis FtsZ

White, E. Lucile, Ross, Larry J., Reynolds, Robert C., Seitz, Lainne E., Moore, Georgia D., Borhani, David W.

The essential cell division protein, FtsZ, from Mycobacterium tuberculosis has been expressed in Escherichia coli and purified. The recombinant protein has GTPase activity typical of tubulin and...

Dynamic control of slow water transport by aquaporin 0: Implications for hydration and junction stability in the eye lens

Jensen, Morten Ø., Dror, Ron O., Xu, Huafeng, Borhani, David W., Arkin, Isaiah T., Eastwood, Michael P., ...

Aquaporin 0 (AQP0), the most abundant membrane protein in mammalian lens fiber cells, not only serves as the primary water channel in this tissue but also appears to mediate the formation of thin...

Identification of two distinct inactive conformations of the β2-adrenergic receptor reconciles structural and biochemical observations

Dror, Ron O., Arlow, Daniel H., Borhani, David W., Jensen, Morten Ø., Piana, Stefano, Shaw, David E.

Fully understanding the mechanisms of signaling proteins such as G protein-coupled receptors (GPCRs) will require the characterization of their conformational states and the pathways connecting those...