Wery, Maxime, Ruidant, Sabine, Schillewaert, Stéphanie, Leporé, Nathalie, Lafontaine, Denis L.J.
Terminal balls detected at the 5′-end of nascent ribosomal transcripts act as pre-rRNA processing complexes and are detected in all eukaryotes examined, resulting in illustrious Christmas tree...
Vanrobays, Emmanuel, Leplus, Alexis, Osheim, Yvonne N., Beyer, Ann L., Wacheul, Ludivine, Lafontaine, Denis L.J.
Eukaryotic ribosome synthesis is a highly dynamic process that involves the transient association of scores of trans-acting factors to nascent pre-ribosomes. Many ribosome synthesis factors are...
Is ribosome synthesis controlled by pol I transcription? (2007)
Chédin, Stéphane, Laferté, Arnaud, Hoang, Tran, Lafontaine, Denis L J, Riva, Michel, Carles, Christophe
Regulation of growth ultimately depends on the control of synthesis of new ribosomes. Ribosome biogenesis is thus a key element of cell biology, which is tightly regulated in response to...
Is ribosome synthesis controlled by pol I transcription? (2007)
Chédin, Stéphane, Laferté, Arnaud, Hoang, Tran, Lafontaine, Denis L J, Riva, Michel, Carles, Christophe
Regulation of growth ultimately depends on the control of synthesis of new ribosomes. Ribosome biogenesis is thus a key element of cell biology, which is tightly regulated in response to...
Dim2p, a KH-domain protein required for small ribosomal subunit synthesis (2004)
VANROBAYS, EMMANUEL, GÉLUGNE, JEAN-PAUL, CAIZERGUES-FERRER, MICHÈLE, LAFONTAINE, DENIS L.J.
Recent proteomic analyses are revealing the dynamics of preribosome assembly. Following cleavage at processing site A2, which generates the 20S pre-rRNA (the immediate precursor to the 18S rRNA),...
Dez, Christophe, Henras, Anthony, Faucon, Bruno, Lafontaine, Denis L. J., Caizergues-Ferrer, Michèle, Henry, Yves
Telomerase is a ribonucleoprotein (RNP) particle required for the replication of telomeres. The RNA component, termed hTR, of human telomerase contains a domain structurally and functionally related...
The box H+ACA snoRNAs carry Cbf5p, the putative rRNA pseudouridine synthase (1998)
Lafontaine, Denis L.J., Bousquet-Antonelli, Cécile, Henry, Yves, Caizergues-Ferrer, Michèle, Tollervey, David
Dez, Christophe, Henras, Anthony, Faucon, Bruno, Lafontaine, Denis L. J., Caizergues-Ferrer, Michèle, Henry, Yves
Telomerase is a ribonucleoprotein (RNP) particle required for the replication of telomeres. The RNA component, termed hTR, of human telomerase contains a domain structurally and functionally related...
Massenet, Séverine, Motorin, Yuri, Lafontaine, Denis L. J., Hurt, Eduard C., Grosjean, Henri, Branlant, Christiane
Pseudouridine (Ψ) residues were localized in the Saccharomyces cerevisiae spliceosomal U small nuclear RNAs (UsnRNAs) by using the chemical mapping method. In contrast to vertebrate UsnRNAs, S....
Synthesis and Assembly of the Box C+D Small Nucleolar RNPs
Lafontaine, Denis L. J., Tollervey, David
Two core small nucleolar RNP (snoRNP) proteins, Nop1p (fibrillarin in vertebrates) and Nop58p (also known as Nop5p) have previously been reported to be specifically associated with the box C+D class...
Kufel, Joanna, Allmang, Christine, Chanfreau, Guillaume, Petfalski, Elisabeth, Lafontaine, Denis L. J., Tollervey, David
Almost all small eukaryotic RNAs are processed from transiently stabilized 3′-extended forms. A key question is how and why such intermediates are stabilized and how they can then be processed to...
Dhr1p, a Putative DEAH-Box RNA Helicase, Is Associated with the Box C+D snoRNP U3
Colley, Alan, Beggs, Jean D., Tollervey, David, Lafontaine, Denis L. J.
Putative RNA helicases are involved in most aspects of gene expression. All previously characterized members of the DEAH-box family of putative RNA helicases are involved in pre-mRNA splicing. Here...
Yeast 18S rRNA Dimethylase Dim1p: a Quality Control Mechanism in Ribosome Synthesis?
Lafontaine, Denis L. J., Preiss, Thomas, Tollervey, David
One of the few rRNA modifications conserved between bacteria and eukaryotes is the base dimethylation present at the 3′ end of the small subunit rRNA. In the yeast Saccharomyces cerevisiae, this...
Verheggen, Céline, Mouaikel, John, Thiry, Marc, Blanchard, Jean-Marie, Tollervey, David, Bordonné, Remi, ...
Nucleolar localization of box C/D small nucleolar (sno) RNAs requires the box C/D motif and, in vertebrates, involves transit through Cajal bodies (CB). We report that in yeast, overexpression of a...
Mammalian and yeast U3 snoRNPs are matured in specific and related nuclear compartments
Verheggen, Céline, Lafontaine, Denis L.J., Samarsky, Dmitry, Mouaikel, John, Blanchard, Jean-Marie, Bordonné, Rémy, ...
Nucleolar localization of vertebrate box C/D snoRNA involves transit through Cajal bodies, but the significance of this event is unkown. To define better the function of this compartment, we analyzed...
The box H+ACA snoRNAs carry Cbf5p, the putative rRNA pseudouridine synthase
Lafontaine, Denis L.J., Bousquet-Antonelli, Cécile, Henry, Yves, Caizergues-Ferrer, Michèle, Tollervey, David
Many or all of the sites of pseudouridine (Ψ) formation in eukaryotic rRNA are selected by site-specific base-pairing with members of the box H + ACA class of small nucleolar RNAs (snoRNAs)....
Colau, Geoffroy, Thiry, Marc, Leduc, Vivian, Bordonné, Rémy, Lafontaine, Denis L. J.
Nucleolar morphogenesis is a poorly defined process. Here we report that the Saccharomyces cerevisiae nucleolar trimethyl guanosine synthase I (Tgs1p), which specifically selects the m7G cap...
Esf2p, a U3-Associated Factor Required for Small-Subunit Processome Assembly and Compaction
Hoang, Tran, Peng, Wen-Tao, Vanrobays, Emmanuel, Krogan, Nevan, Hiley, Shawna, Beyer, Ann L., ...
Esf2p is the Saccharomyces cerevisiae homolog of mouse ABT1, a protein previously identified as a putative partner of the TATA-element binding protein. However, large-scale studies have indicated...
Dim2p, a KH-domain protein required for small ribosomal subunit synthesis
VANROBAYS, EMMANUEL, GÉLUGNE, JEAN-PAUL, CAIZERGUES-FERRER, MICHÈLE, LAFONTAINE, DENIS L.J.
Recent proteomic analyses are revealing the dynamics of preribosome assembly. Following cleavage at processing site A2, which generates the 20S pre-rRNA (the immediate precursor to the 18S rRNA),...
Dez, Christophe, Henras, Anthony, Faucon, Bruno, Lafontaine, Denis L. J., Caizergues-Ferrer, Michèle, Henry, Yves
Telomerase is a ribonucleoprotein (RNP) particle required for the replication of telomeres. The RNA component, termed hTR, of human telomerase contains a domain structurally and functionally related...
Massenet, Séverine, Motorin, Yuri, Lafontaine, Denis L. J., Hurt, Eduard C., Grosjean, Henri, Branlant, Christiane
Pseudouridine (Ψ) residues were localized in the Saccharomyces cerevisiae spliceosomal U small nuclear RNAs (UsnRNAs) by using the chemical mapping method. In contrast to vertebrate UsnRNAs, S....
Synthesis and Assembly of the Box C+D Small Nucleolar RNPs
Lafontaine, Denis L. J., Tollervey, David
Two core small nucleolar RNP (snoRNP) proteins, Nop1p (fibrillarin in vertebrates) and Nop58p (also known as Nop5p) have previously been reported to be specifically associated with the box C+D class...
Kufel, Joanna, Allmang, Christine, Chanfreau, Guillaume, Petfalski, Elisabeth, Lafontaine, Denis L. J., Tollervey, David
Almost all small eukaryotic RNAs are processed from transiently stabilized 3′-extended forms. A key question is how and why such intermediates are stabilized and how they can then be processed to...
Dhr1p, a Putative DEAH-Box RNA Helicase, Is Associated with the Box C+D snoRNP U3
Colley, Alan, Beggs, Jean D., Tollervey, David, Lafontaine, Denis L. J.
Putative RNA helicases are involved in most aspects of gene expression. All previously characterized members of the DEAH-box family of putative RNA helicases are involved in pre-mRNA splicing. Here...
Yeast 18S rRNA Dimethylase Dim1p: a Quality Control Mechanism in Ribosome Synthesis?
Lafontaine, Denis L. J., Preiss, Thomas, Tollervey, David
One of the few rRNA modifications conserved between bacteria and eukaryotes is the base dimethylation present at the 3′ end of the small subunit rRNA. In the yeast Saccharomyces cerevisiae, this...
Verheggen, Céline, Mouaikel, John, Thiry, Marc, Blanchard, Jean-Marie, Tollervey, David, Bordonné, Remi, ...
Nucleolar localization of box C/D small nucleolar (sno) RNAs requires the box C/D motif and, in vertebrates, involves transit through Cajal bodies (CB). We report that in yeast, overexpression of a...
Mammalian and yeast U3 snoRNPs are matured in specific and related nuclear compartments
Verheggen, Céline, Lafontaine, Denis L.J., Samarsky, Dmitry, Mouaikel, John, Blanchard, Jean-Marie, Bordonné, Rémy, ...
Nucleolar localization of vertebrate box C/D snoRNA involves transit through Cajal bodies, but the significance of this event is unkown. To define better the function of this compartment, we analyzed...
The box H+ACA snoRNAs carry Cbf5p, the putative rRNA pseudouridine synthase
Lafontaine, Denis L.J., Bousquet-Antonelli, Cécile, Henry, Yves, Caizergues-Ferrer, Michèle, Tollervey, David
Many or all of the sites of pseudouridine (Ψ) formation in eukaryotic rRNA are selected by site-specific base-pairing with members of the box H + ACA class of small nucleolar RNAs (snoRNAs)....
Colau, Geoffroy, Thiry, Marc, Leduc, Vivian, Bordonné, Rémy, Lafontaine, Denis L. J.
Nucleolar morphogenesis is a poorly defined process. Here we report that the Saccharomyces cerevisiae nucleolar trimethyl guanosine synthase I (Tgs1p), which specifically selects the m7G cap...
Esf2p, a U3-Associated Factor Required for Small-Subunit Processome Assembly and Compaction
Hoang, Tran, Peng, Wen-Tao, Vanrobays, Emmanuel, Krogan, Nevan, Hiley, Shawna, Beyer, Ann L., ...
Esf2p is the Saccharomyces cerevisiae homolog of mouse ABT1, a protein previously identified as a putative partner of the TATA-element binding protein. However, large-scale studies have indicated...
Dim2p, a KH-domain protein required for small ribosomal subunit synthesis
VANROBAYS, EMMANUEL, GÉLUGNE, JEAN-PAUL, CAIZERGUES-FERRER, MICHÈLE, LAFONTAINE, DENIS L.J.
Recent proteomic analyses are revealing the dynamics of preribosome assembly. Following cleavage at processing site A2, which generates the 20S pre-rRNA (the immediate precursor to the 18S rRNA),...
Qiu, Hui, Eifert, Julia, Wacheul, Ludivine, Thiry, Marc, Berger, Adam C., Jakovljevic, Jelena, ...
Small nucleolar RNAs (snoRNAs) orchestrate the modification and cleavage of pre-rRNA and are essential for ribosome biogenesis. Recent data suggest that after nucleoplasmic synthesis, snoRNAs...
Vanrobays, Emmanuel, Leplus, Alexis, Osheim, Yvonne N., Beyer, Ann L., Wacheul, Ludivine, Lafontaine, Denis L.J.
Eukaryotic ribosome synthesis is a highly dynamic process that involves the transient association of scores of trans-acting factors to nascent pre-ribosomes. Many ribosome synthesis factors are...
Wery, Maxime, Ruidant, Sabine, Schillewaert, Stéphanie, Leporé, Nathalie, Lafontaine, Denis L.J.
Terminal balls detected at the 5′-end of nascent ribosomal transcripts act as pre-rRNA processing complexes and are detected in all eukaryotes examined, resulting in illustrious Christmas tree...