Dennis M. Klinman

Multivalent cross-linking of membrane Ig sensitizes murine B cells to a broader spectrum of CpG-containing oligodeoxynucleotide motifs, including their methylated counterparts, for stimulation of proliferation and Ig secretion (1999)

Goeckeritz, Bruce E., Flora, Michael, Witherspoon, Kim, Vos, Quirijn, Lees, Andrew, Dennis, Gregory J., ...

We have previously reported that B cells that are activated by multivalent but not bivalent membrane Ig cross-linking ligands synergize with various B cell activators culminating in enhanced B cell...

Rapid Induction of Protective Immunity Against Biothreat Agents Using CPG-Based Oligonucleotides (1998)

Klinman, Dennis M.

This research project examines the ability of synthetic oligonucleotides (ODN) containing immunostimulatory "CpG motifs' to trigger the innate immune system, thereby improving the host's ability to...

IFN-{gamma}is a potent inducer of Ig secretion by sort-purified murine B cella activated through the mlg, but not the CD40,signaling pathway (1996)

Snapper, Clifford M., Rosas, Fabio, Moorman, Mark A., Jin, Liang, Shanebeck, Kurt, Klinman, Dennis M., ...

To study the influence of the quaternary structure of the outer membrane protein PhoE of Escherlchla coli on the resentation of an inserted T cell epttope, an epttope comprising amino acid residues...

Activation of target-tissue immune-recognition molecules by double-stranded polynucleotides

Suzuki, Koichi, Mori, Atsumi, Ishii, Ken J., Saito, Jun, Singer, Dinah S., Klinman, Dennis M., ...

Abnormal expression of major histocompatibility complex (MHC) class I and class II in various tissues is associated with autoimmune disease. Autoimmune responses can be triggered by viral infections...

Repeated Administration of Synthetic Oligodeoxynucleotides Expressing CpG Motifs Provides Long-Term Protection against Bacterial Infection

Klinman, Dennis M., Conover, Jackie, Coban, Cevahir

Synthetic oligodeoxynucleotides (ODN) expressing unmethylated CpG motifs stimulate an innate immune response characterized by the production of polyreactive immunoglobulin M antibodies and...

CpG Oligodeoxynucleotides and Interleukin-12 Improve the Efficacy of Mycobacterium bovis BCG Vaccination in Mice Challenged with M. tuberculosis

Freidag, Brenda L., Melton, Genevieve B., Collins, Frank, Klinman, Dennis M., Cheever, Allen, Stobie, Laura, ...

Mycobacterium bovis bacillus Calmette-Guérin (BCG) is the only vaccine approved for prevention of tuberculosis. It has been postulated that serial passage of BCG over the years may have resulted in...

Simultaneous Induction of Multiple Antigen-Specific Cytotoxic T Lymphocytes in Nonhuman Primates by Immunization with a Mixture of Four Plasmodium falciparum DNA Plasmids

Wang, Ruobing, Doolan, Denise L., Charoenvit, Yupin, Hedstrom, Richard C., Gardner, Malcolm J., Hobart, Peter, ...

CD8+ T cells have been implicated as critical effector cells in protective immunity against malaria parasites developing within hepatocytes. A vaccine that protects against malaria by inducing CD8+ T...

DNA containing CpG motifs induces angiogenesis

Zheng, Mei, Klinman, Dennis M., Gierynska, Malgorzata, Rouse, Barry T.

New blood vessel formation in the cornea is an essential step in the pathogenesis of a blinding immunoinflammatory reaction caused by ocular infection with herpes simplex virus (HSV). By using a...

Immunoprotective Activity and Safety of a Respiratory Syncytial Virus Vaccine: Mucosal Delivery of Fusion Glycoprotein with a CpG Oligodeoxynucleotide Adjuvant

Prince, Gregory A., Mond, James J., Porter, David D., Yim, Kevin C., Lan, Steve J., Klinman, Dennis M.

CpG oligodeoxynucleotides (ODN) were identified that stimulated immunoglobulin production and cell proliferation in cotton rat cells in vitro. Three of these ODN were used as a mucosal adjuvant in...

Effect of CpG Oligodeoxynucleotides on the Immunogenicity of Pfs25, a Plasmodium falciparum Transmission-Blocking Vaccine Antigen

Coban, Cevayir, Ishii, Ken J., Stowers, Anthony W., Keister, David B., Klinman, Dennis M., Kumar, Nirbhay

Antibodies directed against Pfs25, a protein present on the surface of zygotes and ookinetes of Plasmodium falciparum, completely block pathogen transmission. We evaluated the immunomodulatory effect...

CpG Oligodeoxynucleotides Improve the Survival of Pregnant and Fetal Mice following Listeria monocytogenes Infection

Ito, Shu-ichi, Ishii, Ken J., Shirota, Hidekazu, Klinman, Dennis M.

Listeria infection during pregnancy can cause the death of both mother and fetus. Previous studies established that immunostimulatory CpG oligodeoxynucleotides (ODN) increase the resistance of...

CpG Oligodeoxynucleotides Adsorbed onto Polylactide-Co-Glycolide Microparticles Improve the Immunogenicity and Protective Activity of the Licensed Anthrax Vaccine

Xie, Hang, Gursel, Ihsan, Ivins, Bruce E., Singh, Manmohan, O'Hagan, Derek T., Ulmer, Jeffrey B., ...

To reduce the biothreat posed by anthrax, efforts are under way to improve the protection afforded by vaccination. This work examines the ability of immunostimulatory CpG oligodeoxynucleotides (ODN)...

Contribution of Nitric Oxide to CpG-Mediated Protection against Listeria monocytogenes

Ito, Shuichi, Ishii, Ken J., Ihata, Atsushi, Klinman, Dennis M.

Immunostimulatory CpG oligodeoxynucleotides (ODN) improve host resistance to listeriae. CpG ODN trigger immune cells to produce gamma interferon and “prime” host cells to secrete nitric oxide in...

The adjuvant activity of CpG DNA requires T-bet expression in dendritic cells

Lugo-Villarino, Geanncarlo, Ito, Shu-Ichi, Klinman, Dennis M., Glimcher, Laurie H.

Treatment with synthetic oligodeoxynucleotides containing CpG motifs (CpG ODNs) is remarkably protective against otherwise lethal infection. Here, we describe an essential role for the transcription...

CpG Oligodeoxynucleotides Increase the Susceptibility of Normal Mice to Infection by Candida albicans

Ito, Shuichi, Pedras-Vasconcelos, Joao, Klinman, Dennis M.

Synthetic oligodeoxynucleotides containing CpG motifs trigger an innate immune response that typically increases host resistance to infection. Yet CpG treatment reduces the resistance of normal mice...

Toll-Like Receptor Adaptor Molecules Enhance DNA-Raised Adaptive Immune Responses against Influenza and Tumors through Activation of Innate Immunity

Takeshita, Fumihiko, Tanaka, Toshiyuki, Matsuda, Tomoko, Tozuka, Miyuki, Kobiyama, Kouji, Saha, Sukumar, ...

Toll-like receptors (TLRs) recognize microbial components and trigger the signaling cascade that activates the innate and adaptive immunity. TLR adaptor molecules play a central role in this cascade;...

Activation of target-tissue immune-recognition molecules by double-stranded polynucleotides

Suzuki, Koichi, Mori, Atsumi, Ishii, Ken J., Saito, Jun, Singer, Dinah S., Klinman, Dennis M., ...

Abnormal expression of major histocompatibility complex (MHC) class I and class II in various tissues is associated with autoimmune disease. Autoimmune responses can be triggered by viral infections...

Repeated Administration of Synthetic Oligodeoxynucleotides Expressing CpG Motifs Provides Long-Term Protection against Bacterial Infection

Klinman, Dennis M., Conover, Jackie, Coban, Cevahir

Synthetic oligodeoxynucleotides (ODN) expressing unmethylated CpG motifs stimulate an innate immune response characterized by the production of polyreactive immunoglobulin M antibodies and...

CpG Oligodeoxynucleotides and Interleukin-12 Improve the Efficacy of Mycobacterium bovis BCG Vaccination in Mice Challenged with M. tuberculosis

Freidag, Brenda L., Melton, Genevieve B., Collins, Frank, Klinman, Dennis M., Cheever, Allen, Stobie, Laura, ...

Mycobacterium bovis bacillus Calmette-Guérin (BCG) is the only vaccine approved for prevention of tuberculosis. It has been postulated that serial passage of BCG over the years may have resulted in...

Simultaneous Induction of Multiple Antigen-Specific Cytotoxic T Lymphocytes in Nonhuman Primates by Immunization with a Mixture of Four Plasmodium falciparum DNA Plasmids

Wang, Ruobing, Doolan, Denise L., Charoenvit, Yupin, Hedstrom, Richard C., Gardner, Malcolm J., Hobart, Peter, ...

CD8+ T cells have been implicated as critical effector cells in protective immunity against malaria parasites developing within hepatocytes. A vaccine that protects against malaria by inducing CD8+ T...

DNA containing CpG motifs induces angiogenesis

Zheng, Mei, Klinman, Dennis M., Gierynska, Malgorzata, Rouse, Barry T.

New blood vessel formation in the cornea is an essential step in the pathogenesis of a blinding immunoinflammatory reaction caused by ocular infection with herpes simplex virus (HSV). By using a...

Immunoprotective Activity and Safety of a Respiratory Syncytial Virus Vaccine: Mucosal Delivery of Fusion Glycoprotein with a CpG Oligodeoxynucleotide Adjuvant

Prince, Gregory A., Mond, James J., Porter, David D., Yim, Kevin C., Lan, Steve J., Klinman, Dennis M.

CpG oligodeoxynucleotides (ODN) were identified that stimulated immunoglobulin production and cell proliferation in cotton rat cells in vitro. Three of these ODN were used as a mucosal adjuvant in...

Effect of CpG Oligodeoxynucleotides on the Immunogenicity of Pfs25, a Plasmodium falciparum Transmission-Blocking Vaccine Antigen

Coban, Cevayir, Ishii, Ken J., Stowers, Anthony W., Keister, David B., Klinman, Dennis M., Kumar, Nirbhay

Antibodies directed against Pfs25, a protein present on the surface of zygotes and ookinetes of Plasmodium falciparum, completely block pathogen transmission. We evaluated the immunomodulatory effect...

CpG Oligodeoxynucleotides Improve the Survival of Pregnant and Fetal Mice following Listeria monocytogenes Infection

Ito, Shu-ichi, Ishii, Ken J., Shirota, Hidekazu, Klinman, Dennis M.

Listeria infection during pregnancy can cause the death of both mother and fetus. Previous studies established that immunostimulatory CpG oligodeoxynucleotides (ODN) increase the resistance of...

CpG Oligodeoxynucleotides Adsorbed onto Polylactide-Co-Glycolide Microparticles Improve the Immunogenicity and Protective Activity of the Licensed Anthrax Vaccine

Xie, Hang, Gursel, Ihsan, Ivins, Bruce E., Singh, Manmohan, O'Hagan, Derek T., Ulmer, Jeffrey B., ...

To reduce the biothreat posed by anthrax, efforts are under way to improve the protection afforded by vaccination. This work examines the ability of immunostimulatory CpG oligodeoxynucleotides (ODN)...

Contribution of Nitric Oxide to CpG-Mediated Protection against Listeria monocytogenes

Ito, Shuichi, Ishii, Ken J., Ihata, Atsushi, Klinman, Dennis M.

Immunostimulatory CpG oligodeoxynucleotides (ODN) improve host resistance to listeriae. CpG ODN trigger immune cells to produce gamma interferon and “prime” host cells to secrete nitric oxide in...

The adjuvant activity of CpG DNA requires T-bet expression in dendritic cells

Lugo-Villarino, Geanncarlo, Ito, Shu-Ichi, Klinman, Dennis M., Glimcher, Laurie H.

Treatment with synthetic oligodeoxynucleotides containing CpG motifs (CpG ODNs) is remarkably protective against otherwise lethal infection. Here, we describe an essential role for the transcription...

CpG Oligodeoxynucleotides Increase the Susceptibility of Normal Mice to Infection by Candida albicans

Ito, Shuichi, Pedras-Vasconcelos, Joao, Klinman, Dennis M.

Synthetic oligodeoxynucleotides containing CpG motifs trigger an innate immune response that typically increases host resistance to infection. Yet CpG treatment reduces the resistance of normal mice...

Toll-Like Receptor Adaptor Molecules Enhance DNA-Raised Adaptive Immune Responses against Influenza and Tumors through Activation of Innate Immunity

Takeshita, Fumihiko, Tanaka, Toshiyuki, Matsuda, Tomoko, Tozuka, Miyuki, Kobiyama, Kouji, Saha, Sukumar, ...

Toll-like receptors (TLRs) recognize microbial components and trigger the signaling cascade that activates the innate and adaptive immunity. TLR adaptor molecules play a central role in this cascade;...

Contribution of interferon-β to the immune activation induced by double-stranded DNA

Shirota, Hidekazu, Ishii, Ken J, Takakuwa, Hiroki, Klinman, Dennis M

Introducing double-stranded DNA (dsDNA) into the cytoplasm of macrophages and dendritic cells triggers the activation of these professional antigen-presenting cells (APCs). This process is...

Potential Role of Phosphatidylinositol 3 Kinase, rather than DNA-dependent Protein Kinase, in CpG DNA–induced Immune Activation

Ishii, Ken J., Takeshita, Fumihiko, Gursel, Ihsan, Gursel, Mayda, Conover, Jacqueline, Nussenzweig, Andre, ...

Unmethylated CpG motifs present in bacterial DNA stimulate a strong innate immune response. There is evidence that DNA-dependent protein kinase (DNA-PK) mediates CpG signaling. Specifically,...

Vaccination with Heat-killed Leishmania Antigen or Recombinant Leishmanial Protein and CpG Oligodeoxynucleotides Induces Long-Term Memory CD4+and CD8+T Cell Responses and Protection Against Leishmania major Infection

Rhee, Elizabeth G., Mendez, Susana, Shah, Javeed A., Wu, Chang-you, Kirman, Joanna R., Turon, Tara N., ...

CpG oligodeoxynucleotides (ODN) have potent effects on innate and adaptive cellular immune responses. In this report, the ability of CpG ODN to confer long-term immunity and protection when used as a...

Interferon (IFN) Consensus Sequence-binding Protein, a Transcription Factor of the IFN Regulatory Factor Family, Regulates Immune Responses In Vivo through Control of Interleukin 12 Expression

Giese, Nathalia A., Gabriele, Lucia, Doherty, T. Mark, Klinman, Dennis M., Tadesse-Heath, Lekidelu, Contursi, Christina, ...

Mice with a null mutation of the gene encoding interferon consensus sequence-binding protein (ICSBP) develop a chronic myelogenous leukemia-like syndrome and mount impaired responses to certain viral...

Toll-like receptor ligands synergize through distinct dendritic cell pathways to induce T cell responses: Implications for vaccines

Zhu, Qing, Egelston, Colt, Vivekanandhan, Aravindhan, Uematsu, Satoshi, Akira, Shizuo, Klinman, Dennis M., ...

Toll-like receptors (TLRs) may need to cooperate with each other to be effective in detecting imminent infection and trigger immune responses. Understanding is still limited about the intracellular...