Duenas-Decamp, Maria J., Peters, Paul J., Burton, Dennis R., Clapham, Paul R.
The human monoclonal antibody b12 recognizes a conserved epitope on gp120 that overlaps the CD4 binding site. b12 has neutralizing activity against diverse human immunodeficiency virus type 1 (HIV-1)...
Peters, Paul J., Duenas-Decamp, Maria J., Sullivan, W. Matthew, Brown, Richard J. P., Ankghuambom, Chiambah, Luzuriaga, Katherine, ...
BACKGROUND: HIV-1 R5 viruses cause most of the AIDS cases worldwide and are preferentially transmitted compared to CXCR4-using viruses. Furthermore, R5 viruses vary extensively in capacity to infect...
Peters, Paul J, Duenas-Decamp, Maria J, Sullivan, W Matthew, Brown, Richard, Ankghuambom, Chiambah, Luzuriaga, Katherine, ...
Abstract Background HIV-1 R5 viruses cause most of the AIDS cases worldwide and are preferentially transmitted compared to CXCR4-using viruses. Furthermore, R5 viruses vary extensively in capacity to...
Binley, James M, Ngo-Abdalla, Stacie, Moore, Penny, Bobardt, Michael, Chatterji, Udayan, Gallay, Philippe, ...
No abstract available.
Binley, James M, Ngo-Abdalla, Stacie, Moore, Penny, Bobardt, Michael, Chatterji, Udayan, Gallay, Philippe, ...
No abstract available.
Neutralizing Antibody Fails to Impact the Course of Ebola Virus Infection in Monkeys (2007)
Wendelien B. Oswald, Thomas W. Geisbert, Kelly J. Davis, Joan B. Geisbert, Nancy J. Sullivan, Peter B. Jahrling, ...
Prophylaxis with high doses of neutralizing antibody typically offers protection against challenge with viruses producing acute infections. In this study, we have investigated the ability of the...
Design clues from functional constraints and broadly neutralizing antibodies (2006)
Zhou, Tongqing, Xu, Ling, Dey, Barna, Hessell, Ann J, Majeed, Shahzad, Van Ryk, Donald, ...
No abstract available.
Binley, James M, Ngo-Abdalla, Stacie, Moore, Penny, Bobardt, Michael, Chatterji, Udayan, Gallay, Philippe, ...
Abstract During natural HIV infection, an array of host receptors are thought to influence virus attachment and the kinetics of infection. In this study, to probe the interactions of HIV envelope...
Strain-specified relative conformational stability of the scrapie prion protein (2001)
Peretz, David, Scott, Michael R., Groth, Darlene, Williamson, R. Anthony, Burton, Dennis R., Cohen, Fred E., ...
Studies of prion biology and diseases have elucidated several new concepts, but none was more heretical than the proposal that the biological properties that distinguish different prion strains are...
Joakim Glamann, Dennis R. Burton, Henrik J. Ditzel, Karen A. Kent, ...
An antibody phage display library was constructed from RNA extracted from lymph node cells of a simian immunodeficiency virus (SIV)-infected long-term-nonprogressor macaque. Seven gp120-reactive Fabs...
The interaction of polyamines with DNA: a 23Na NMR study (1981)
Burton, Dennis R., Forsén, Sture, Reimarsson, Pétur
The interaction between a variety of polyamines, both naturally occurring and synthetic, and calf thymus DNA has been studied using 23Na NMR. The relaxation behaviour of 23Na reflects the extent of...
Metal ions as nuclear magnetic resonance probes in biology / (1978)
Thesis (doctoral)--University of Lund, 1978.
Rapid antibody responses by low-dose, single-step, dendritic cell-targeted immunization
Wang, Hui, Griffiths, Michelle N., Burton, Dennis R., Ghazal, Peter
We have compared the kinetics of antibody responses in conventional and dendritic cell-targeted immunization by using a model antigen in mice. Targeting was achieved by linking the reporter antigen...
Cloning and expression of a novel human antibody–antigen pair associated with Felty's syndrome
Ditzel, Henrik J., Masaki, Yasufumi, Nielsen, Heidi, Farnaes, Lauge, Burton, Dennis R.
An increasing number of studies suggest the importance of antibodies in the pathogenesis of most systemic and organ-specific autoimmune diseases, although there is considerable controversy over the...
Ditzel, Henrik J., Rosenkilde, Mette M., Garred, Peter, Wang, Meng, Koefoed, Klaus, Pedersen, Court, ...
The chemokine receptor CCR5 is the major coreceptor for infection by macrophage-tropic R5 HIV-1. A 32-bp deletion in the gene coding for CCR5 (CCR5Δ32) occurs with a frequency of 10% in the...
Anti-DNA antibodies are a major component of the intrathecal B cell response in multiple sclerosis
Williamson, R. Anthony, Burgoon, Mark P., Owens, Gregory P., Ghausi, Omar, Leclerc, Estelle, Firme, Louise, ...
Multiple sclerosis (MS) is a chronic inflammatory demyelinating disease of unknown cause that afflicts the central nervous system. MS is typified by a highly clonally restricted antigen-driven...
A vaccine for HIV type 1: The antibody perspective
Antibodies that bind well to the envelope spikes of immunodeficiency viruses such as HIV type 1 (HIV-1) and simian immunodeficiency virus (SIV) can offer protection or benefit if present at...
Sakurai, Hiroshi, Williamson, R. Anthony, Crowe, James E., Beeler, Judy A., Poignard, Pascal, Bastidas, Raiza B., ...
A number of antibodies generated during human respiratory syncytial virus (RSV) infection have been cloned by the phage library approach. Antibodies reactive with an immunodominant epitope on the F...
De Logu, Alessandro, Williamson, R. Anthony, Rozenshteyn, Roman, Ramiro-Ibañez, Fernando, Simpson, Cindy D., Burton, Dennis R., ...
We report the characterization of a type-common human recombinant monoclonal antibody previously isolated by antigen selection from a phage-displayed combinatorial antibody library established from a...
Glamann, Joakim, Burton, Dennis R., Ditzel, Henrik J., Kent, Karen A., Arnold, Caroline, ...
An antibody phage display library was constructed from RNA extracted from lymph node cells of a simian immunodeficiency virus (SIV)-infected long-term-nonprogressor macaque. Seven gp120-reactive Fabs...
Mondor, Isabelle, Naniche, Denise, Ditzel, Henrik J., Klasse, P. J., Burton, Dennis R., ...
We investigated the relative importance of binding site occupancy and epitope specificity in antibody neutralization of human immunodeficiency virus (HIV) type 1 (HIV-1). The neutralization of a...
Sullivan, Nancy, Sun, Ying, Binley, James, Lee, Juliette, Barbas, Carlos F., ...
Infection by some human immunodeficiency virus type 1 (HIV-1) isolates is enhanced by the binding of subneutralizing concentrations of soluble receptor, soluble CD4 (sCD4), or monoclonal antibodies...
Mapping the Prion Protein Using Recombinant Antibodies
Williamson, R. Anthony, Peretz, David, Pinilla, Clemencia, Ball, Hadyn, Bastidas, Raiza B., Rozenshteyn, Roman, ...
The fundamental event in prion disease is thought to be the posttranslational conversion of the cellular prion protein (PrPC) into a pathogenic isoform (PrPSc). The occurrence of PrPC on the cell...
Frankel, Sarah S., Steinman, Ralph M., Michael, Nelson L., Kim, Silvia Ratto, Bhardwaj, Nina, Pope, Melissa, ...
Prevention of the initial infection of mucosal dendritic cells (DC) and interruption of the subsequent transmission of HIV-1 from DC to T cells are likely to be important attributes of an effective...
Antibody Neutralization-Resistant Primary Isolates of Human Immunodeficiency Virus Type 1
Wang, Meng, Trkola, Alexandra, Binley, James M., Purtscher, Martin, Katinger, Hermann, ...
Although typical primary isolates of human immunodeficiency virus type 1 (HIV-1) are relatively neutralization resistant, three human monoclonal antibodies and a small number of HIV-1+ human sera...
Owens, Gregory P., Williamson, R. Anthony, Burgoon, Mark P., Ghausi, Omar, Burton, Dennis R., Gilden, Donald H.
In central nervous system (CNS) infectious and inflammatory diseases of known cause, oligoclonal bands represent antibody directed against the causative agent. To determine whether disease-relevant...
Ebola Virus Can Be Effectively Neutralized by Antibody Produced in Natural Human Infection
Maruyama, Toshiaki, Rodriguez, Luis L., Jahrling, Peter B., Sanchez, Anthony, Khan, Ali S., Nichol, Stuart T., ...
The activity of antibodies against filoviruses is poorly understood but has important consequences for vaccine design and passive prophylaxis. To investigate this activity, a panel of recombinant...
Characterization of Primary Isolate-Like Variants of Simian-Human Immunodeficiency Virus
Crawford, John M., Earl, Patricia L., Moss, Bernard, Reimann, Keith A., Wyand, Michael S., Manson, Kelledy H., ...
Several different strains of simian-human immunodeficiency virus (SHIV) that contain the envelope glycoproteins of either T-cell-line-adapted (TCLA) strains or primary isolates of human...
Zwick, Michael B., Bonnycastle, Lori L. C., Menendez, Alfredo, Irving, Melita B., Barbas, Carlos F., ...
Human monoclonal antibody (MAb) b12 recognizes a conformational epitope that overlaps the CD-4-binding site of the human immunodeficiency virus type 1 (HIV-1) envelope. MAb b12 neutralizes a broad...
Zwick, Michael B., Labrijn, Aran F., Wang, Meng, Spenlehauer, Catherine, Saphire, Erica Ollmann, Binley, James M., ...
The identification and epitope mapping of broadly neutralizing anti-human immunodeficiency virus type 1 (HIV-1) antibodies (Abs) is important for vaccine design, but, despite much effort, very few...
Marx, Preston A., Hessell, Ann J., Luckay, Amara, Harouse, Janet, Cheng-Mayer, Cecilia, ...
A major unknown in human immunodeficiency virus (HIV-1) vaccine design is the efficacy of antibodies in preventing mucosal transmission of R5 viruses. These viruses, which use CCR5 as a coreceptor,...
Zwick, Michael B., Wang, Meng, Poignard, Pascal, Stiegler, Gabriela, Katinger, Hermann, Burton, Dennis R., ...
Several reports have described the existence of synergy between neutralizing monoclonal antibodies (MAbs) against human immunodeficiency virus type 1 (HIV-1). Synergy between human MAbs b12, 2G12,...
Moulard, Maxime, Phogat, Sanjay K., Shu, Yuuei, Labrijn, Aran F., Xiao, Xiaodong, Binley, James M., ...
HIV-1 entry into cells involves formation of a complex between gp120 of the viral envelope glycoprotein (Env), a receptor (CD4), and a coreceptor, typically CCR5. Here we provide evidence that...
Neutralizing Human Fab Fragments against Measles Virus Recovered by Phage Display
De Carvalho Nicacio, Cristina, Williamson, R. Anthony, Lundkvist, Åke, Burton, Dennis R., Björling, Ewa
Five human recombinant Fab fragments (Fabs) specific for measles virus (MV) proteins were isolated from three antibody phage display libraries generated from RNAs derived from bone marrow or splenic...
Enhancing the Proteolytic Maturation of Human Immunodeficiency Virus Type 1 Envelope Glycoproteins
Binley, James M., Sanders, Rogier W., Master, Aditi, Cayanan, Charmagne S., Wiley, Cheryl L., Schiffner, Linnea, ...
In virus-infected cells, the envelope glycoprotein (Env) precursor, gp160, of human immunodeficiency virus type 1 is cleaved by cellular proteases into a fusion-competent gp120-gp41 heterodimer in...
Geisbert, Tom W., Maruyama, Toshiaki, Jahrling, Peter B., Burton, Dennis R.
A neutralizing human monoclonal antibody, KZ52, protects guinea pigs from lethal Ebola Zaire virus challenge. Administration before or up to 1 h after challenge resulted in dose-dependent protection...
Scanlan, Christopher N., Pantophlet, Ralph, Wormald, Mark R., Ollmann Saphire, Erica, Stanfield, Robyn, Wilson, Ian A., ...
2G12 is a broadly neutralizing human monoclonal antibody against human immunodeficiency virus type-1 (HIV-1) that has previously been shown to bind to a carbohydrate-dependent epitope on gp120. Here,...
Venturini, Sara, Mosier, Donald E., Burton, Dennis R., Poignard, Pascal
Substantial evidence argues that human immunodeficiency virus type 1 (HIV-1)-specific CD4+ T cells play an important role in the control of HIV-1 replication in infected individuals. Moreover, it is...
Poignard, Pascal, Moulard, Maxime, Golez, Edwin, Vivona, Veronique, Franti, Michael, Venturini, Sara, ...
Virion capture assays, in which immobilized antibodies (Abs) capture virus particles, have been used to suggest that nonneutralizing Abs bind effectively to human immunodeficiency virus type 1...
Pantophlet, Ralph, Ollmann Saphire, Erica, Poignard, Pascal, Wilson, Ian A., Burton, Dennis R.
Alanine scanning mutagenesis was performed on monomeric gp120 of human immunodeficiency virus type 1 to systematically identify residues important for gp120 recognition by neutralizing and...
Herrera, Carolina, Spenlehauer, Catherine, Fung, Michael S., Burton, Dennis R., Beddows, Simon, Moore, John P.
We have investigated whether nonneutralizing monoclonal antibodies (MAbs) to the gp120 subunit of the envelope glycoprotein (Env) complex of human immunodeficiency virus type 1 (HIV-1) can interfere...
Leclerc, Estelle, Peretz, David, Ball, Haydn, Sakurai, Hiroshi, Legname, Giuseppe, Serban, Ana, ...
It is hypothesized that infectious prions are generated as the cellular form of the prion protein (PrPC) undergoes pronounced conformational change under the direction of an infectious PrPSc...
Zwick, Michael B., Saphire, Erica O., Church, Sarah, Wang, Meng, Scott, Jamie K., ...
IgG1 b12 is a broadly neutralizing antibody against human immunodeficiency virus type 1 (HIV-1). The epitope recognized by b12 overlaps the CD4 receptor-binding site (CD4bs) on gp120 and has been a...
Pantophlet, Ralph, Wilson, Ian A., Burton, Dennis R.
The ability to induce broadly neutralizing antibodies should be a key component of any forthcoming vaccine against human immunodeficiency virus type 1. One potential vaccine candidate, monomeric...
Redox-Triggered Infection by Disulfide-Shackled Human Immunodeficiency Virus Type 1 Pseudovirions
Binley, James M., Cayanan, Charmagne S., Wiley, Cheryl, Schülke, Norbert, Olson, William C., Burton, Dennis R.
We previously described a human immunodeficiency virus type 1 (HIV-1) envelope mutant that introduces a disulfide bridge between the gp120 surface proteins and gp41 transmembrane proteins (J. M....
Zwick, Michael B., Kelleher, Robert, Jensen, Richard, Labrijn, Aran F., Wang, Meng, ...
The V1/V2 and V3 loops are proximal to the CD4 binding site (CD4bs) of human immunodeficiency virus type 1 (HIV-1) gp120 and undergo conformational change upon CD4 receptor engagement by the HIV-1...
O'Connor, David H., Mothe, Bianca R., Weinfurter, Jason T., Fuenger, Sarah, Rehrauer, William M., Jing, Peicheng, ...
Certain major histocompatibility complex class I (MHC-I) alleles are associated with delayed disease progression in individuals infected with human immunodeficiency virus (HIV) and in macaques...
Johnson, Welkin E., Sanford, Hannah, Schwall, Linda, Burton, Dennis R., Robinson, James E., ...
Simian immunodeficiency virus (SIV) of macaques isolate SIVmac239 is highly resistant to neutralization by polyclonal antisera or monoclonal antibodies, a property that it shares with most primary...
Labrijn, Aran F., Poignard, Pascal, Raja, Aarti, Zwick, Michael B., Delgado, Karla, Franti, Michael, ...
Anti-human immunodeficiency virus type 1 (HIV-1) antibodies whose binding to gp120 is enhanced by CD4 binding (CD4i antibodies) are generally considered nonneutralizing for primary HIV-1 isolates....
Mascola, John R., Lewis, Mark G., VanCott, Thomas C., Stiegler, Gabriela, Katinger, Hermann, Seaman, Michael, ...
High levels of infused anti-human immunodeficiency virus type 1 (HIV-1) neutralizing monoclonal antibodies (MAbs) can completely protect macaque monkeys against mucosal chimeric simian-human...
Zwick, Michael B., Komori, H. Kiyomi, Stanfield, Robyn L., Church, Sarah, Wang, Meng, ...
The human monoclonal antibody 2F5 neutralizes primary human immunodeficiency virus type 1 (HIV-1) with rare breadth and potency. A crystal structure of a complex of 2F5 and a peptide corresponding to...
Motif-grafted antibodies containing the replicative interface of cellular PrP are specific for PrPSc
Moroncini, Gianluca, Kanu, Nnennaya, Solforosi, Laura, Abalos, Gil, Telling, Glenn C., Head, Mark, ...
Prion diseases are closely associated with the conversion of the cellular prion protein (PrPC) to an abnormal conformer (PrPSc) [Prusiner, S. B. (1998) Proc. Natl. Acad. Sci. USA 95, 13363–13383]....
Zhang, Mei-Yun, Xiao, Xiaodong, Sidorov, Igor A., Choudhry, Vidita, Cham, Fatim, Zhang, Peng Fei, ...
The identification and characterization of new human monoclonal antibodies (hMAbs) able to neutralize primary human immunodeficiency virus type 1 (HIV-1) isolates from different subtypes may help in...
Binley, James M., Wrin, Terri, Korber, Bette, Zwick, Michael B., Wang, Meng, Chappey, Colombe, ...
Broadly neutralizing monoclonal antibodies (MAbs) are potentially important tools in human immunodeficiency virus type 1 (HIV-1) vaccine design. A few rare MAbs have been intensively studied, but we...
A Dominant Role for CD8+-T-Lymphocyte Selection in Simian Immunodeficiency Virus Sequence Variation†
O'Connor, David H., McDermott, Adrian B., Krebs, Kendall C., Dodds, Elizabeth J., Miller, Jacqueline E., Gonzalez, Edna J., ...
CD8+ T lymphocytes (CD8-TL) select viral escape variants in both human immunodeficiency virus and simian immunodeficiency virus (SIV) infections. The frequency of CD8-TL viral escape as well as the...
Printed covalent glycan array for ligand profiling of diverse glycan binding proteins
Blixt, Ola, Head, Steve, Mondala, Tony, Scanlan, Christopher, Huflejt, Margaret E., Alvarez, Richard, ...
Here we describe a glycan microarray constructed by using standard robotic microarray printing technology to couple amine functionalized glycans to an amino-reactive glass slide. The array comprises...
Zwick, Michael B., Jensen, Richard, Church, Sarah, Wang, Meng, Stiegler, Gabriela, Kunert, Renate, ...
The conserved membrane-proximal external region (MPER) of human immunodeficiency virus type 1 (HIV-1) gp41 is a target of two broadly neutralizing human monoclonal antibodies, 2F5 and 4E10, and is an...
Wahl-Jensen, Victoria, Kurz, Sabine K., Hazelton, Paul R., Schnittler, Hans-Joachim, Ströher, Ute, Burton, Dennis R., ...
Ebola virus, a member of the family Filoviridae, causes one of the most severe forms of viral hemorrhagic fever. In the terminal stages of disease, symptoms progress to hypotension, coagulation...
Robust hepatitis C virus infection in vitro
Zhong, Jin, Gastaminza, Pablo, Cheng, Guofeng, Kapadia, Sharookh, Kato, Takanobu, Burton, Dennis R., ...
The absence of a robust cell culture model of hepatitis C virus (HCV) infection has severely limited analysis of the HCV life cycle and the development of effective antivirals and vaccines. Here we...
Comparing Antigenicity and Immunogenicity of Engineered gp120
Selvarajah, Suganya, Puffer, Bridget, Pantophlet, Ralph, Law, Mansun, Doms, Robert W., Burton, Dennis R.
We have engineered monomeric gp120 in such a way as to favorably present the conserved epitope for the broadly neutralizing antibody b12 while lowering the exposure of epitopes recognized by some...
Dissection of the carbohydrate specificity of the broadly neutralizing anti-HIV-1 antibody 2G12
Calarese, Daniel A., Lee, Hing-Ken, Huang, Cheng-Yuan, Best, Michael D., Astronomo, Rena D., Stanfield, Robyn L., ...
Human antibody 2G12 neutralizes a broad range of HIV-1 isolates. Hence, molecular characterization of its epitope, which corresponds to a conserved cluster of oligomannoses on the viral envelope...
Zhang, Mason X., Bohlman, M. Charlotte, Itatani, Carol, Burton, Dennis R., St. Jeor, Stephen C., ...
Mannan is a major cell wall component found in Candida species. Natural antimannan antibody is present in sera from most normal adults, but its role in host resistance to hematogenously disseminated...
Antibody vs. HIV in a clash of evolutionary titans
Burton, Dennis R., Stanfield, Robyn L., Wilson, Ian A.
HIV has evolved many strategies to avoid neutralizing antibody responses, particularly to conserved regions on the external glycoprotein spikes of the virus. Nevertheless, a small number of...
Brunel, Florence M., Zwick, Michael B., Cardoso, Rosa M. F., Nelson, Josh D., Wilson, Ian A., Burton, Dennis R., ...
The human immunodeficiency virus type 1 (HIV-1) neutralizing antibody 4E10 binds to a linear, highly conserved epitope within the membrane-proximal external region of the HIV-1 envelope glycoprotein...
Nature of Nonfunctional Envelope Proteins on the Surface of Human Immunodeficiency Virus Type 1
Moore, Penny L., Crooks, Emma T., Porter, Lauren, Zhu, Ping, Cayanan, Charmagne S., Grise, Henry, ...
Human immunodeficiency virus type 1 (HIV-1) neutralizing antibodies are thought be distinguished from nonneutralizing antibodies by their ability to recognize functional gp120/gp41 envelope...
Yuste, Eloisa, Sanford, Hannah B., Carmody, Jill, Bixby, Jacqueline, Little, Susan, Zwick, Michael B., ...
To date, only a small number of anti-human immunodeficiency virus type 1 (HIV-1) monoclonal antibodies (MAbs) with relatively broad neutralizing activity have been isolated from infected individuals....
Binley, James M, Ngo-Abdalla, Stacie, Moore, Penny, Bobardt, Michael, Chatterji, Udayan, Gallay, Philippe, ...
During natural HIV infection, an array of host receptors are thought to influence virus attachment and the kinetics of infection. In this study, to probe the interactions of HIV envelope (Env) with...
Wilson, Nancy A., Reed, Jason, Napoe, Gnankang S., Piaskowski, Shari, Szymanski, Andy, Furlott, Jessica, ...
The goal of an AIDS vaccine regimen designed to induce cellular immune responses should be to reduce the viral set point and preserve memory CD4 lymphocytes. Here we investigated whether...
Rapid antibody responses by low-dose, single-step, dendritic cell-targeted immunization
Wang, Hui, Griffiths, Michelle N., Burton, Dennis R., Ghazal, Peter
We have compared the kinetics of antibody responses in conventional and dendritic cell-targeted immunization by using a model antigen in mice. Targeting was achieved by linking the reporter antigen...
Cloning and expression of a novel human antibody–antigen pair associated with Felty's syndrome
Ditzel, Henrik J., Masaki, Yasufumi, Nielsen, Heidi, Farnaes, Lauge, Burton, Dennis R.
An increasing number of studies suggest the importance of antibodies in the pathogenesis of most systemic and organ-specific autoimmune diseases, although there is considerable controversy over the...
Ditzel, Henrik J., Rosenkilde, Mette M., Garred, Peter, Wang, Meng, Koefoed, Klaus, Pedersen, Court, ...
The chemokine receptor CCR5 is the major coreceptor for infection by macrophage-tropic R5 HIV-1. A 32-bp deletion in the gene coding for CCR5 (CCR5Δ32) occurs with a frequency of 10% in the...
Anti-DNA antibodies are a major component of the intrathecal B cell response in multiple sclerosis
Williamson, R. Anthony, Burgoon, Mark P., Owens, Gregory P., Ghausi, Omar, Leclerc, Estelle, Firme, Louise, ...
Multiple sclerosis (MS) is a chronic inflammatory demyelinating disease of unknown cause that afflicts the central nervous system. MS is typified by a highly clonally restricted antigen-driven...
A vaccine for HIV type 1: The antibody perspective
Antibodies that bind well to the envelope spikes of immunodeficiency viruses such as HIV type 1 (HIV-1) and simian immunodeficiency virus (SIV) can offer protection or benefit if present at...
Sakurai, Hiroshi, Williamson, R. Anthony, Crowe, James E., Beeler, Judy A., Poignard, Pascal, Bastidas, Raiza B., ...
A number of antibodies generated during human respiratory syncytial virus (RSV) infection have been cloned by the phage library approach. Antibodies reactive with an immunodominant epitope on the F...
De Logu, Alessandro, Williamson, R. Anthony, Rozenshteyn, Roman, Ramiro-Ibañez, Fernando, Simpson, Cindy D., Burton, Dennis R., ...
We report the characterization of a type-common human recombinant monoclonal antibody previously isolated by antigen selection from a phage-displayed combinatorial antibody library established from a...
Glamann, Joakim, Burton, Dennis R., Ditzel, Henrik J., Kent, Karen A., Arnold, Caroline, ...
An antibody phage display library was constructed from RNA extracted from lymph node cells of a simian immunodeficiency virus (SIV)-infected long-term-nonprogressor macaque. Seven gp120-reactive Fabs...
Mondor, Isabelle, Naniche, Denise, Ditzel, Henrik J., Klasse, P. J., Burton, Dennis R., ...
We investigated the relative importance of binding site occupancy and epitope specificity in antibody neutralization of human immunodeficiency virus (HIV) type 1 (HIV-1). The neutralization of a...
Sullivan, Nancy, Sun, Ying, Binley, James, Lee, Juliette, Barbas, Carlos F., ...
Infection by some human immunodeficiency virus type 1 (HIV-1) isolates is enhanced by the binding of subneutralizing concentrations of soluble receptor, soluble CD4 (sCD4), or monoclonal antibodies...
Mapping the Prion Protein Using Recombinant Antibodies
Williamson, R. Anthony, Peretz, David, Pinilla, Clemencia, Ball, Hadyn, Bastidas, Raiza B., Rozenshteyn, Roman, ...
The fundamental event in prion disease is thought to be the posttranslational conversion of the cellular prion protein (PrPC) into a pathogenic isoform (PrPSc). The occurrence of PrPC on the cell...
Frankel, Sarah S., Steinman, Ralph M., Michael, Nelson L., Kim, Silvia Ratto, Bhardwaj, Nina, Pope, Melissa, ...
Prevention of the initial infection of mucosal dendritic cells (DC) and interruption of the subsequent transmission of HIV-1 from DC to T cells are likely to be important attributes of an effective...
Antibody Neutralization-Resistant Primary Isolates of Human Immunodeficiency Virus Type 1
Wang, Meng, Trkola, Alexandra, Binley, James M., Purtscher, Martin, Katinger, Hermann, ...
Although typical primary isolates of human immunodeficiency virus type 1 (HIV-1) are relatively neutralization resistant, three human monoclonal antibodies and a small number of HIV-1+ human sera...
Owens, Gregory P., Williamson, R. Anthony, Burgoon, Mark P., Ghausi, Omar, Burton, Dennis R., Gilden, Donald H.
In central nervous system (CNS) infectious and inflammatory diseases of known cause, oligoclonal bands represent antibody directed against the causative agent. To determine whether disease-relevant...
Ebola Virus Can Be Effectively Neutralized by Antibody Produced in Natural Human Infection
Maruyama, Toshiaki, Rodriguez, Luis L., Jahrling, Peter B., Sanchez, Anthony, Khan, Ali S., Nichol, Stuart T., ...
The activity of antibodies against filoviruses is poorly understood but has important consequences for vaccine design and passive prophylaxis. To investigate this activity, a panel of recombinant...
Characterization of Primary Isolate-Like Variants of Simian-Human Immunodeficiency Virus
Crawford, John M., Earl, Patricia L., Moss, Bernard, Reimann, Keith A., Wyand, Michael S., Manson, Kelledy H., ...
Several different strains of simian-human immunodeficiency virus (SHIV) that contain the envelope glycoproteins of either T-cell-line-adapted (TCLA) strains or primary isolates of human...
Zwick, Michael B., Bonnycastle, Lori L. C., Menendez, Alfredo, Irving, Melita B., Barbas, Carlos F., ...
Human monoclonal antibody (MAb) b12 recognizes a conformational epitope that overlaps the CD-4-binding site of the human immunodeficiency virus type 1 (HIV-1) envelope. MAb b12 neutralizes a broad...
Zwick, Michael B., Labrijn, Aran F., Wang, Meng, Spenlehauer, Catherine, Saphire, Erica Ollmann, Binley, James M., ...
The identification and epitope mapping of broadly neutralizing anti-human immunodeficiency virus type 1 (HIV-1) antibodies (Abs) is important for vaccine design, but, despite much effort, very few...
Marx, Preston A., Hessell, Ann J., Luckay, Amara, Harouse, Janet, Cheng-Mayer, Cecilia, ...
A major unknown in human immunodeficiency virus (HIV-1) vaccine design is the efficacy of antibodies in preventing mucosal transmission of R5 viruses. These viruses, which use CCR5 as a coreceptor,...
Zwick, Michael B., Wang, Meng, Poignard, Pascal, Stiegler, Gabriela, Katinger, Hermann, Burton, Dennis R., ...
Several reports have described the existence of synergy between neutralizing monoclonal antibodies (MAbs) against human immunodeficiency virus type 1 (HIV-1). Synergy between human MAbs b12, 2G12,...
Moulard, Maxime, Phogat, Sanjay K., Shu, Yuuei, Labrijn, Aran F., Xiao, Xiaodong, Binley, James M., ...
HIV-1 entry into cells involves formation of a complex between gp120 of the viral envelope glycoprotein (Env), a receptor (CD4), and a coreceptor, typically CCR5. Here we provide evidence that...
Neutralizing Human Fab Fragments against Measles Virus Recovered by Phage Display
De Carvalho Nicacio, Cristina, Williamson, R. Anthony, Lundkvist, Åke, Burton, Dennis R., Björling, Ewa
Five human recombinant Fab fragments (Fabs) specific for measles virus (MV) proteins were isolated from three antibody phage display libraries generated from RNAs derived from bone marrow or splenic...
Enhancing the Proteolytic Maturation of Human Immunodeficiency Virus Type 1 Envelope Glycoproteins
Binley, James M., Sanders, Rogier W., Master, Aditi, Cayanan, Charmagne S., Wiley, Cheryl L., Schiffner, Linnea, ...
In virus-infected cells, the envelope glycoprotein (Env) precursor, gp160, of human immunodeficiency virus type 1 is cleaved by cellular proteases into a fusion-competent gp120-gp41 heterodimer in...
Geisbert, Tom W., Maruyama, Toshiaki, Jahrling, Peter B., Burton, Dennis R.
A neutralizing human monoclonal antibody, KZ52, protects guinea pigs from lethal Ebola Zaire virus challenge. Administration before or up to 1 h after challenge resulted in dose-dependent protection...
Scanlan, Christopher N., Pantophlet, Ralph, Wormald, Mark R., Ollmann Saphire, Erica, Stanfield, Robyn, Wilson, Ian A., ...
2G12 is a broadly neutralizing human monoclonal antibody against human immunodeficiency virus type-1 (HIV-1) that has previously been shown to bind to a carbohydrate-dependent epitope on gp120. Here,...
Venturini, Sara, Mosier, Donald E., Burton, Dennis R., Poignard, Pascal
Substantial evidence argues that human immunodeficiency virus type 1 (HIV-1)-specific CD4+ T cells play an important role in the control of HIV-1 replication in infected individuals. Moreover, it is...
Poignard, Pascal, Moulard, Maxime, Golez, Edwin, Vivona, Veronique, Franti, Michael, Venturini, Sara, ...
Virion capture assays, in which immobilized antibodies (Abs) capture virus particles, have been used to suggest that nonneutralizing Abs bind effectively to human immunodeficiency virus type 1...
Pantophlet, Ralph, Ollmann Saphire, Erica, Poignard, Pascal, Wilson, Ian A., Burton, Dennis R.
Alanine scanning mutagenesis was performed on monomeric gp120 of human immunodeficiency virus type 1 to systematically identify residues important for gp120 recognition by neutralizing and...
Herrera, Carolina, Spenlehauer, Catherine, Fung, Michael S., Burton, Dennis R., Beddows, Simon, Moore, John P.
We have investigated whether nonneutralizing monoclonal antibodies (MAbs) to the gp120 subunit of the envelope glycoprotein (Env) complex of human immunodeficiency virus type 1 (HIV-1) can interfere...
Leclerc, Estelle, Peretz, David, Ball, Haydn, Sakurai, Hiroshi, Legname, Giuseppe, Serban, Ana, ...
It is hypothesized that infectious prions are generated as the cellular form of the prion protein (PrPC) undergoes pronounced conformational change under the direction of an infectious PrPSc...
Zwick, Michael B., Saphire, Erica O., Church, Sarah, Wang, Meng, Scott, Jamie K., ...
IgG1 b12 is a broadly neutralizing antibody against human immunodeficiency virus type 1 (HIV-1). The epitope recognized by b12 overlaps the CD4 receptor-binding site (CD4bs) on gp120 and has been a...
Pantophlet, Ralph, Wilson, Ian A., Burton, Dennis R.
The ability to induce broadly neutralizing antibodies should be a key component of any forthcoming vaccine against human immunodeficiency virus type 1. One potential vaccine candidate, monomeric...
Redox-Triggered Infection by Disulfide-Shackled Human Immunodeficiency Virus Type 1 Pseudovirions
Binley, James M., Cayanan, Charmagne S., Wiley, Cheryl, Schülke, Norbert, Olson, William C., Burton, Dennis R.
We previously described a human immunodeficiency virus type 1 (HIV-1) envelope mutant that introduces a disulfide bridge between the gp120 surface proteins and gp41 transmembrane proteins (J. M....
Zwick, Michael B., Kelleher, Robert, Jensen, Richard, Labrijn, Aran F., Wang, Meng, ...
The V1/V2 and V3 loops are proximal to the CD4 binding site (CD4bs) of human immunodeficiency virus type 1 (HIV-1) gp120 and undergo conformational change upon CD4 receptor engagement by the HIV-1...
O'Connor, David H., Mothe, Bianca R., Weinfurter, Jason T., Fuenger, Sarah, Rehrauer, William M., Jing, Peicheng, ...
Certain major histocompatibility complex class I (MHC-I) alleles are associated with delayed disease progression in individuals infected with human immunodeficiency virus (HIV) and in macaques...
Johnson, Welkin E., Sanford, Hannah, Schwall, Linda, Burton, Dennis R., Robinson, James E., ...
Simian immunodeficiency virus (SIV) of macaques isolate SIVmac239 is highly resistant to neutralization by polyclonal antisera or monoclonal antibodies, a property that it shares with most primary...
Labrijn, Aran F., Poignard, Pascal, Raja, Aarti, Zwick, Michael B., Delgado, Karla, Franti, Michael, ...
Anti-human immunodeficiency virus type 1 (HIV-1) antibodies whose binding to gp120 is enhanced by CD4 binding (CD4i antibodies) are generally considered nonneutralizing for primary HIV-1 isolates....
Mascola, John R., Lewis, Mark G., VanCott, Thomas C., Stiegler, Gabriela, Katinger, Hermann, Seaman, Michael, ...
High levels of infused anti-human immunodeficiency virus type 1 (HIV-1) neutralizing monoclonal antibodies (MAbs) can completely protect macaque monkeys against mucosal chimeric simian-human...
Zwick, Michael B., Komori, H. Kiyomi, Stanfield, Robyn L., Church, Sarah, Wang, Meng, ...
The human monoclonal antibody 2F5 neutralizes primary human immunodeficiency virus type 1 (HIV-1) with rare breadth and potency. A crystal structure of a complex of 2F5 and a peptide corresponding to...
Motif-grafted antibodies containing the replicative interface of cellular PrP are specific for PrPSc
Moroncini, Gianluca, Kanu, Nnennaya, Solforosi, Laura, Abalos, Gil, Telling, Glenn C., Head, Mark, ...
Prion diseases are closely associated with the conversion of the cellular prion protein (PrPC) to an abnormal conformer (PrPSc) [Prusiner, S. B. (1998) Proc. Natl. Acad. Sci. USA 95, 13363–13383]....
Zhang, Mei-Yun, Xiao, Xiaodong, Sidorov, Igor A., Choudhry, Vidita, Cham, Fatim, Zhang, Peng Fei, ...
The identification and characterization of new human monoclonal antibodies (hMAbs) able to neutralize primary human immunodeficiency virus type 1 (HIV-1) isolates from different subtypes may help in...
Binley, James M., Wrin, Terri, Korber, Bette, Zwick, Michael B., Wang, Meng, Chappey, Colombe, ...
Broadly neutralizing monoclonal antibodies (MAbs) are potentially important tools in human immunodeficiency virus type 1 (HIV-1) vaccine design. A few rare MAbs have been intensively studied, but we...
A Dominant Role for CD8+-T-Lymphocyte Selection in Simian Immunodeficiency Virus Sequence Variation†
O'Connor, David H., McDermott, Adrian B., Krebs, Kendall C., Dodds, Elizabeth J., Miller, Jacqueline E., Gonzalez, Edna J., ...
CD8+ T lymphocytes (CD8-TL) select viral escape variants in both human immunodeficiency virus and simian immunodeficiency virus (SIV) infections. The frequency of CD8-TL viral escape as well as the...
Printed covalent glycan array for ligand profiling of diverse glycan binding proteins
Blixt, Ola, Head, Steve, Mondala, Tony, Scanlan, Christopher, Huflejt, Margaret E., Alvarez, Richard, ...
Here we describe a glycan microarray constructed by using standard robotic microarray printing technology to couple amine functionalized glycans to an amino-reactive glass slide. The array comprises...
Zwick, Michael B., Jensen, Richard, Church, Sarah, Wang, Meng, Stiegler, Gabriela, Kunert, Renate, ...
The conserved membrane-proximal external region (MPER) of human immunodeficiency virus type 1 (HIV-1) gp41 is a target of two broadly neutralizing human monoclonal antibodies, 2F5 and 4E10, and is an...
Wahl-Jensen, Victoria, Kurz, Sabine K., Hazelton, Paul R., Schnittler, Hans-Joachim, Ströher, Ute, Burton, Dennis R., ...
Ebola virus, a member of the family Filoviridae, causes one of the most severe forms of viral hemorrhagic fever. In the terminal stages of disease, symptoms progress to hypotension, coagulation...
Robust hepatitis C virus infection in vitro
Zhong, Jin, Gastaminza, Pablo, Cheng, Guofeng, Kapadia, Sharookh, Kato, Takanobu, Burton, Dennis R., ...
The absence of a robust cell culture model of hepatitis C virus (HCV) infection has severely limited analysis of the HCV life cycle and the development of effective antivirals and vaccines. Here we...
Comparing Antigenicity and Immunogenicity of Engineered gp120
Selvarajah, Suganya, Puffer, Bridget, Pantophlet, Ralph, Law, Mansun, Doms, Robert W., Burton, Dennis R.
We have engineered monomeric gp120 in such a way as to favorably present the conserved epitope for the broadly neutralizing antibody b12 while lowering the exposure of epitopes recognized by some...
Dissection of the carbohydrate specificity of the broadly neutralizing anti-HIV-1 antibody 2G12
Calarese, Daniel A., Lee, Hing-Ken, Huang, Cheng-Yuan, Best, Michael D., Astronomo, Rena D., Stanfield, Robyn L., ...
Human antibody 2G12 neutralizes a broad range of HIV-1 isolates. Hence, molecular characterization of its epitope, which corresponds to a conserved cluster of oligomannoses on the viral envelope...
Antibody vs. HIV in a clash of evolutionary titans
Burton, Dennis R., Stanfield, Robyn L., Wilson, Ian A.
HIV has evolved many strategies to avoid neutralizing antibody responses, particularly to conserved regions on the external glycoprotein spikes of the virus. Nevertheless, a small number of...
Zhang, Mason X., Bohlman, M. Charlotte, Itatani, Carol, Burton, Dennis R., St. Jeor, Stephen C., ...
Mannan is a major cell wall component found in Candida species. Natural antimannan antibody is present in sera from most normal adults, but its role in host resistance to hematogenously disseminated...
Brunel, Florence M., Zwick, Michael B., Cardoso, Rosa M. F., Nelson, Josh D., Wilson, Ian A., Burton, Dennis R., ...
The human immunodeficiency virus type 1 (HIV-1) neutralizing antibody 4E10 binds to a linear, highly conserved epitope within the membrane-proximal external region of the HIV-1 envelope glycoprotein...
Nature of Nonfunctional Envelope Proteins on the Surface of Human Immunodeficiency Virus Type 1
Moore, Penny L., Crooks, Emma T., Porter, Lauren, Zhu, Ping, Cayanan, Charmagne S., Grise, Henry, ...
Human immunodeficiency virus type 1 (HIV-1) neutralizing antibodies are thought be distinguished from nonneutralizing antibodies by their ability to recognize functional gp120/gp41 envelope...
Yuste, Eloisa, Sanford, Hannah B., Carmody, Jill, Bixby, Jacqueline, Little, Susan, Zwick, Michael B., ...
To date, only a small number of anti-human immunodeficiency virus type 1 (HIV-1) monoclonal antibodies (MAbs) with relatively broad neutralizing activity have been isolated from infected individuals....
Wilson, Nancy A., Reed, Jason, Napoe, Gnankang S., Piaskowski, Shari, Szymanski, Andy, Furlott, Jessica, ...
The goal of an AIDS vaccine regimen designed to induce cellular immune responses should be to reduce the viral set point and preserve memory CD4 lymphocytes. Here we investigated whether...
Binley, James M, Ngo-Abdalla, Stacie, Moore, Penny, Bobardt, Michael, Chatterji, Udayan, Gallay, Philippe, ...
During natural HIV infection, an array of host receptors are thought to influence virus attachment and the kinetics of infection. In this study, to probe the interactions of HIV envelope (Env) with...
Design clues from functional constraints and broadly neutralizing antibodies
Zhou, Tongqing, Xu, Ling, Dey, Barna, Hessell, Ann J, Majeed, Shahzad, Van Ryk, Donald, ...
Neutralizing Antibody Fails to Impact the Course of Ebola Virus Infection in Monkeys
Oswald, Wendelien B, Geisbert, Thomas W, Davis, Kelly J, Geisbert, Joan B, Sullivan, Nancy J, Jahrling, Peter B, ...
Prophylaxis with high doses of neutralizing antibody typically offers protection against challenge with viruses producing acute infections. In this study, we have investigated the ability of the...
Maruyama, Toshiaki, Saito, Ichiro, Hayashi, Yoshio, Kompfner, Elizabeth, Fox, Robert I., Burton, Dennis R., ...
Lymphocyte infiltration of salivary and lacrimal glands leading to diminished secretion and gland destruction as a result of apoptosis is thought to be pivotal in the pathogenesis of Sjögren’s...
Binley, James M, Ngo-Abdalla, Stacie, Moore, Penny, Bobardt, Michael, Chatterji, Udayan, Gallay, Philippe, ...
Nelson, Josh D., Brunel, Florence M., Jensen, Richard, Crooks, Emma T., Cardoso, Rosa M. F., Wang, Meng, ...
The membrane-proximal external region (MPER) of human immunodeficiency virus type 1 (HIV-1) gp41 bears the epitopes of two broadly neutralizing antibodies (Abs), 2F5 and 4E10, making it a target for...
Law, Mansun, Cardoso, Rosa M. F., Wilson, Ian A., Burton, Dennis R.
The membrane-proximal external region (MPER) of human immunodeficiency virus type 1 (HIV-1) gp41 is a target of broadly neutralizing monoclonal antibodies (MAbs) 2F5, 4E10, and Z13. Here we engrafted...
Dhillon, Amandeep K., Donners, Helen, Pantophlet, Ralph, Johnson, Welkin E., Decker, Julie M., Shaw, George M., ...
Attempts to elicit broadly neutralizing antibody responses by human immunodeficiency virus type 1 (HIV-1) vaccine antigens have been met with limited success. To better understand the requirements...
Williams, John V., Chen, Zhifeng, Cseke, Gabriella, Wright, David W., Keefer, Christopher J., Tollefson, Sharon J., ...
Human metapneumovirus (hMPV) is a recently discovered paramyxovirus that is a major cause of lower-respiratory-tract disease. hMPV is associated with more severe disease in infants and persons with...
Immune responses and HIV: a little order from the chaos
O'Connor, David H., Burton, Dennis R.
HIV is evolution gone mad and bad. The virus infects a person and rapidly diversifies to become a huge swarm of viruses, each equipped differently to resist the onslaught of diverse T cells and...
Peters, Paul J, Duenas-Decamp, Maria J, Sullivan, W Matthew, Brown, Richard, Ankghuambom, Chiambah, Luzuriaga, Katherine, ...
Ugolini, Sophie, Mondor, Isabelle, Burton, Dennis R., Tilley, Shermaine A., Klasse, Per Johan, ...
Antibody-mediated neutralization of human immunodeficiency virus type–1 (HIV-1) is thought to function by at least two distinct mechanisms: inhibition of virus–receptor binding, and interference...
Trafficking of prion proteins through a caveolae-mediated endosomal pathway
Peters, Peter J., Mironov, Alexander, Peretz, David, Van Donselaar, Elly, Leclerc, Estelle, Erpel, Susanne, ...
To understand the posttranslational conversion of the cellular prion protein (PrPC) to its pathologic conformation, it is important to define the intracellular trafficking pathway of PrPC within the...
Strain-specified relative conformational stability of the scrapie prion protein
Peretz, David, Scott, Michael R., Groth, Darlene, Williamson, R. Anthony, Burton, Dennis R., Cohen, Fred E., ...
Studies of prion biology and diseases have elucidated several new concepts, but none was more heretical than the proposal that the biological properties that distinguish different prion strains are...
Wang, Sheng-Kai, Liang, Pi-Hui, Astronomo, Rena D., Hsu, Tsui-Ling, Hsieh, Shie-Liang, Burton, Dennis R., ...
It is widely accepted that the heavily glycosylated glycoprotein gp120 on the surface of HIV-1 shields peptide epitopes from recognition by the immune system and may promote infection in vivo by...
Astronomo, Rena D., Lee, Hing-Ken, Scanlan, Christopher N., Pantophlet, Ralph, Huang, Cheng-Yuan, Wilson, Ian A., ...
The glycan shield of human immunodeficiency virus type 1 (HIV-1) gp120 contributes to viral evasion from humoral immune responses. However, the shield is recognized by the HIV-1 broadly neutralizing...
Macaques vaccinated with live-attenuated SIV control replication of heterologous virus
Reynolds, Matthew R., Weiler, Andrea M., Weisgrau, Kim L., Piaskowski, Shari M., Furlott, Jessica R., Weinfurter, Jason T., ...
An effective AIDS vaccine will need to protect against globally diverse isolates of HIV. To address this issue in macaques, we administered a live-attenuated simian immunodeficiency virus (SIV)...
Hessell, Ann J., Rakasz, Eva G., Poignard, Pascal, Hangartner, Lars, Landucci, Gary, Forthal, Donald N., ...
Developing an immunogen that elicits broadly neutralizing antibodies (bNAbs) is an elusive but important goal of HIV vaccine research, especially after the recent failure of the leading T cell based...
Libraries against libraries for combinatorial selection of replicating antigen–antibody pairs
Bowley, Diana R., Jones, Teresa M., Burton, Dennis R., Lerner, Richard A.
Antibodies are among the most highly selective tight-binding ligands for proteins. Because the human genome project has deciphered the proteome, there is an opportunity to use combinatorial antibody...
Pantophlet, Ralph, Wang, Meng, Aguilar-Sino, Rowena O., Burton, Dennis R.
The human immunodeficiency virus type 1 (HIV-1) envelope spike is a heavily glycosylated trimeric structure in which protein surfaces conserved between different HIV-1 isolates are particularly well...
Wu, Lan, Zhou, Tongqing, Yang, Zhi-yong, Svehla, Krisha, O'Dell, Sijy, Louder, Mark K., ...
The broadly neutralizing antibody immunoglobulin G1 (IgG1) b12 binds to a conformationally conserved surface on the outer domain of the human immunodeficiency virus type 1 (HIV-1) gp120 envelope...