Deyin Guo

Construction of an Artificial MicroRNA Expression Vector for Simultaneous Inhibition of Multiple Genes in Mammalian Cells (2009)

Tao Hu, Qiong Fu, Ping Chen, Li Ma, Onsam Sin, Deyin Guo

Recently, artificial microRNA (amiRNA) has become a promising RNA interference (RNAi) technology. Here, we describe a flexible and reliable method for constructing both single- and multi-amiRNA...

Molekularbiologische Charakterisierung der RNA3 und deren subgenomischer RNA des Prunus-Necrotic-Ringspot-Virus / (1995)

Guo, Deyin.

Zugl.: Braunschweig, TU, Diss., 1995.

Identification of Novel Subgenomic RNAs and Noncanonical Transcription Initiation Signals of Severe Acute Respiratory Syndrome Coronavirus

Hussain, Snawar, Pan, Ji'an, Chen, Yu, Yang, Yalin, Xu, Jing, Peng, Yu, ...

The expression of the genomic information of severe acute respiratory syndrome coronavirus (SARS CoV) involves synthesis of a nested set of subgenomic RNAs (sgRNAs) by discontinuous transcription. In...

Identification of Novel Subgenomic RNAs and Noncanonical Transcription Initiation Signals of Severe Acute Respiratory Syndrome Coronavirus

Hussain, Snawar, Pan, Ji'an, Chen, Yu, Yang, Yalin, Xu, Jing, Peng, Yu, ...

The expression of the genomic information of severe acute respiratory syndrome coronavirus (SARS CoV) involves synthesis of a nested set of subgenomic RNAs (sgRNAs) by discontinuous transcription. In...

Genome-Wide Analysis of Protein-Protein Interactions and Involvement of Viral Proteins in SARS-CoV Replication

Pan, Ji'An, Peng, Xiaoxue, Gao, Yajing, Li, Zhilin, Lu, Xiaolu, Chen, Yingzhao, ...

Analyses of viral protein-protein interactions are an important step to understand viral protein functions and their underlying molecular mechanisms. In this study, we adopted a mammalian two-hybrid...

Construction of an Artificial MicroRNA Expression Vector for Simultaneous Inhibition of Multiple Genes in Mammalian Cells

Hu, Tao, Fu, Qiong, Chen, Ping, Ma, Li, Sin, Onsam, Guo, Deyin

Recently, artificial microRNA (amiRNA) has become a promising RNA interference (RNAi) technology. Here, we describe a flexible and reliable method for constructing both single- and multi-amiRNA...

Functional screen reveals SARS coronavirus nonstructural protein nsp14 as a novel cap N7 methyltransferase

Chen, Yu, Cai, Hui, Pan, Ji'an, Xiang, Nian, Tien, Po, Ahola, Tero, ...

The N7-methylguanosine (m7G) cap is the defining structural feature of eukaryotic mRNAs. Most eukaryotic viruses that replicate in the cytoplasm, including coronaviruses, have evolved strategies to...