Didier Blanot

Design and Synthesis of Novel N-Benzylidenesulfonohydrazide Inhibitors of MurC and MurD as Potential Antibacterial Agents (2008)

Rok Frlan, Andreja Kovač, Didier Blanot, Stanislav Gobec, Slavko Pečar, Aleš Obreza

A series of novel N-benzylidenesulfonohydrazide compounds were designedand synthesized as inhibitors of UDP-N-acetylmuramic acid:L-alanine ligase (MurC) andUDP-N-acetylmuramoyl-L-alanine:D-glutamate...

Glycosyl transferase activity of the Escherichia coli penicillin-binding protein 1b: Specificity profile for the substrate (2006)

Fraipont, Claudine, Sapunaric, Frédéric, Zervosen, Astrid, Auger, Geneviève, Devreese, Bart, Lioux, Thierry, ...

The glycosyl transferase of the Escherichia coli bifunctional penicillin-binding protein (PBP) 1b catalyzes the assembly of lipid-transported...

Toll-like receptor 2-dependent bacterial sensing does not occur via peptidoglycan recognition. (2004)

Travassos, Leonardo H, Girardin, Stephen E, Philpott, Dana J, Blanot, Didier, Nahori, Marie-Anne, Werts, Catherine, ...

Toll-like receptor 2 (TLR2) has been shown to recognize several classes of pathogen-associated molecular patterns including peptidoglycan (PG). However, studies linking PG with TLR2 recognition have...

Toll-like receptor 2-dependent bacterial sensing does not occur via peptidoglycan recognition. (2004)

Travassos, Leonardo H, Girardin, Stephen E, Philpott, Dana J, Blanot, Didier, Nahori, Marie-Anne, Werts, Catherine, ...

Toll-like receptor 2 (TLR2) has been shown to recognize several classes of pathogen-associated molecular patterns including peptidoglycan (PG). However, studies linking PG with TLR2 recognition have...

Expression of the Staphylococcus aureus UDP-N-Acetylmuramoyl- l-Alanyl-d-Glutamate:l-Lysine Ligase in Escherichia coli and Effects on Peptidoglycan Biosynthesis and Cell Growth

Mengin-Lecreulx, Dominique, Falla, Tim, Blanot, Didier, Van Heijenoort, Jean, Adams, David J., Chopra, Ian

The monomer units in the Escherichia coli and Staphylococcus aureus cell wall peptidoglycans differ in the nature of the third amino acid in the l-alanyl-γ-d-glutamyl-X-d-alanyl-d-alanine side...

Functional and Biochemical Analysis of Chlamydia trachomatis MurC, an Enzyme Displaying UDP-N-Acetylmuramate:Amino Acid Ligase Activity

Hesse, Lars, Bostock, Julieanne, Dementin, Sebastien, Blanot, Didier, Mengin-Lecreulx, Dominique, Chopra, Ian

Chlamydiae are unusual obligate intracellular bacteria that cause serious infections in humans. Chlamydiae contain genes that appear to encode products with peptidoglycan biosynthetic activity. The...

Fluorescence Detection-Based Functional Assay for High-Throughput Screening for MraY

Stachyra, Thérèse, Dini, Christophe, Ferrari, Paul, Bouhss, Ahmed, Van Heijenoort, Jean, Mengin-Lecreulx, Dominique, ...

We have developed a novel assay specific to MraY, which catalyzes the first membrane step in the biosynthesis of bacterial cell wall peptidoglycan. This was accomplished by using...

Glycopeptide Resistance vanA Operons in Paenibacillus Strains Isolated from Soil

Guardabassi, Luca, Perichon, Bruno, Van Heijenoort, Jean, Blanot, Didier, Courvalin, Patrice

The sequence and gene organization of the van operons in vancomycin (MIC of >256 μg/ml)- and teicoplanin (MIC of ≥32 μg/ml)-resistant Paenibacillus thiaminolyticus PT-2B1 and Paenibacillus...

Toll-like receptor 2-dependent bacterial sensing does not occur via peptidoglycan recognition

Travassos, Leonardo H, Girardin, Stephen E, Philpott, Dana J, Blanot, Didier, Nahori, Marie-Anne, Werts, Catherine, ...

Toll-like receptor 2 (TLR2) has been shown to recognize several classes of pathogen-associated molecular patterns including peptidoglycan (PG). However, studies linking PG with TLR2 recognition have...

Expression of the Staphylococcus aureus UDP-N-Acetylmuramoyl- l-Alanyl-d-Glutamate:l-Lysine Ligase in Escherichia coli and Effects on Peptidoglycan Biosynthesis and Cell Growth

Mengin-Lecreulx, Dominique, Falla, Tim, Blanot, Didier, Van Heijenoort, Jean, Adams, David J., Chopra, Ian

The monomer units in the Escherichia coli and Staphylococcus aureus cell wall peptidoglycans differ in the nature of the third amino acid in the l-alanyl-γ-d-glutamyl-X-d-alanyl-d-alanine side...

Functional and Biochemical Analysis of Chlamydia trachomatis MurC, an Enzyme Displaying UDP-N-Acetylmuramate:Amino Acid Ligase Activity

Hesse, Lars, Bostock, Julieanne, Dementin, Sebastien, Blanot, Didier, Mengin-Lecreulx, Dominique, Chopra, Ian

Chlamydiae are unusual obligate intracellular bacteria that cause serious infections in humans. Chlamydiae contain genes that appear to encode products with peptidoglycan biosynthetic activity. The...

Fluorescence Detection-Based Functional Assay for High-Throughput Screening for MraY

Stachyra, Thérèse, Dini, Christophe, Ferrari, Paul, Bouhss, Ahmed, Van Heijenoort, Jean, Mengin-Lecreulx, Dominique, ...

We have developed a novel assay specific to MraY, which catalyzes the first membrane step in the biosynthesis of bacterial cell wall peptidoglycan. This was accomplished by using...

Glycopeptide Resistance vanA Operons in Paenibacillus Strains Isolated from Soil

Guardabassi, Luca, Perichon, Bruno, Van Heijenoort, Jean, Blanot, Didier, Courvalin, Patrice

The sequence and gene organization of the van operons in vancomycin (MIC of >256 μg/ml)- and teicoplanin (MIC of ≥32 μg/ml)-resistant Paenibacillus thiaminolyticus PT-2B1 and Paenibacillus...

Toll-like receptor 2-dependent bacterial sensing does not occur via peptidoglycan recognition

Travassos, Leonardo H, Girardin, Stephen E, Philpott, Dana J, Blanot, Didier, Nahori, Marie-Anne, Werts, Catherine, ...

Toll-like receptor 2 (TLR2) has been shown to recognize several classes of pathogen-associated molecular patterns including peptidoglycan (PG). However, studies linking PG with TLR2 recognition have...

Biochemical Characterization and Physiological Properties of Escherichia coli UDP-N-Acetylmuramate:l-Alanyl-γ-d-Glutamyl-meso- Diaminopimelate Ligase▿

Hervé, Mireille, Boniface, Audrey, Gobec, Stanislav, Blanot, Didier, Mengin-Lecreulx, Dominique

The UDP-N-acetylmuramate:l-alanyl-γ-d-glutamyl-meso-diaminopimelate ligase (murein peptide ligase [Mpl]) is known to be a recycling enzyme allowing reincorporation into peptidoglycan (murein) of the...

The GTPase CpgA Is Implicated in the Deposition of the Peptidoglycan Sacculus in Bacillus subtilis▿

Absalon, Cédric, Hamze, Kassem, Blanot, Didier, Frehel, Claude, Carballido-Lopez, Rut, Holland, Barry I., ...

Depletion of the Bacillus subtilis GTPase CpgA produces abnormal cell shapes, nonuniform deposition of cell wall, and five- to sixfold accumulation of peptidoglycan precursors. Nevertheless, the...

Glucosamine Found as a Substituent of Both Phosphate Groups in Bordetella Lipid A Backbones: Role of a BvgAS-Activated ArnT Ortholog▿

Marr, Nico, Tirsoaga, Alina, Blanot, Didier, Fernandez, Rachel, Caroff, Martine

Endotoxins are amphipathic lipopolysaccharides (LPSs), major constituents of the outer membrane of gram-negative bacteria. They consist of a lipid region, covalently linked to a core oligosaccharide,...

The Peptidoglycan of Stationary-Phase Mycobacterium tuberculosis Predominantly Contains Cross-Links Generated by l,d-Transpeptidation▿

Lavollay, Marie, Arthur, Michel, Fourgeaud, Martine, Dubost, Lionel, Marie, Arul, Veziris, Nicolas, ...

Our understanding of the mechanisms used by Mycobacterium tuberculosis to persist in a “dormant” state is essential to the development of therapies effective in sterilizing tissues. Gene...

The Peptidoglycan Sacculus of Myxococcus xanthus Has Unusual Structural Features and Is Degraded during Glycerol-Induced Myxospore Development▿

Bui, Nhat Khai, Gray, Joe, Schwarz, Heinz, Schumann, Peter, Blanot, Didier, Vollmer, Waldemar

Upon nutrient limitation cells of the swarming soil bacterium Myxococcus xanthus form a multicellular fruiting body in which a fraction of the cells develop into myxospores. Spore development...