Geiser, Andrew H., Sievert, Michael K., Guo, Lian-Wang, Grant, Jennifer E., Krebs, Mark P., Fotiadis, Dimitrios, ...
The mechanisms by which G-protein-coupled receptors (GPCRs) activate G-proteins are not well understood due to the lack of atomic structures of GPCRs in an active form or in GPCR/G-protein complexes....
Determining molecular forces that stabilize human aquaporin-1. (2003)
Moller, Clemens, Fotiadis, Dimitrios, Suda, Kitaru, Engel, Andreas, Kessler, Max, Muller, Daniel J
Atomic force microscopy (AFM) was used to measure the forces stabilizing human aquaporin-1 (hAQP1), a tetrameric transmembrane protein that forms highly specific water channels. To this end, the AFM...
Imaging and manipulation of biological structures with the AFM (2002)
Fotiadis, Dimitrios, Scheuring, Simon, Müller, Shirley A., Engel, Andreas, Müller, Daniel J.
Many biologists have dreamt of physically touching and manipulating the biomolecules they were investigating. With the invention of the atomic force microscope (AFM), this dream has come true. Here,...
Identification and structure of a putative Ca2+-binding domain at the C terminus of AQP1 (2002)
Fotiadis, Dimitrios, Suda, Kitaru, Tittmann, Peter, Jeno, Paul, Philippsen, Ansgar, Müller, Daniel J., ...
um in translocation of AQP1 in cholangiocytes from intracellular vesicles to the plasma membrane and in triggering its fusion is discussed. Functional studies are now required to identify the...
Biochemical and structural analyses of membrane proteins in plants and animals / (2000)
Fotiadis, Dimitrios (Dimitrios José)
Diss. Naturwiss. Basel (kein Austausch).
Molecular architecture of photosynthetic membranes in Rhodobacter sphaeroides: the role of PufX
Siebert, C Alistair, Qian, Pu, Fotiadis, Dimitrios, Engel, Andreas, Hunter, C Neil, Bullough, Per A
The effects of the PufX polypeptide on membrane architecture were investigated by comparing the composition and structures of photosynthetic membranes from PufX+ and PufX− strains of Rhodobacter...
Bacterial Na+-ATP synthase has an undecameric rotor
Stahlberg, Henning, Müller, Daniel J., Suda, Kitaru, Fotiadis, Dimitrios, Engel, Andreas, Meier, Thomas, ...
Synthesis of adenosine triphosphate (ATP) by the F1F0 ATP synthase involves a membrane-embedded rotary engine, the F0 domain, which drives the extra-membranous catalytic F1 domain. The F0 domain...
Electron and atomic force microscopy of the trimeric ammonium transporter AmtB
Conroy, Matthew J, Jamieson, Stuart J, Blakey, Daniel, Kaufmann, Thomas, Engel, Andreas, Fotiadis, Dimitrios, ...
Escherichia coli AmtB is an archetypal member of the ammonium transporter (Amt) family, a family of proteins that are conserved in all domains of life. Reconstitution of AmtB in the presence of...
Rhodopsin Signaling and Organization in Heterozygote Rhodopsin Knockout Mice*S
Liang, Yan, Fotiadis, Dimitrios, Maeda, Tadao, Maeda, Akiko, Modzelewska, Anna, Filipek, Slawomir, ...
Rhodopsin (Rho) resides within internal membrane structures called disc membranes that are found in the rod outer segments (ROS) of photoreceptors in the retina. Rho expression is essential for...
The supramolecular structure of the GPCR rhodopsin in solution and native disc membranes
Suda, Kitaru, Filipek, Slawomir, Palczewski, Krzysztof, Engel, Andreas, Fotiadis, Dimitrios
Rhodopsin, the prototypical G-protein-coupled receptor, which is densely packed in the disc membranes of rod outer segments, was proposed to function as a monomer. However, a growing body of evidence...
Functional Characterization of Rhodopsin Monomers and Dimers in Detergents*S
Jastrzebska, Beata, Maeda, Tadao, Zhu, Li, Fotiadis, Dimitrios, Filipek, Slawomir, Engel, Andreas, ...
Rhodopsin (Rho) is a G protein-coupled receptor that initiates phototransduction in rod photoreceptors. High expression levels of Rho in the disc membranes of rod outer segments and the propensity of...
Organization of the G Protein-coupled Receptors Rhodopsin and Opsin in Native Membranes*
Liang, Yan, Fotiadis, Dimitrios, Filipek, Sławomir, Saperstein, David A., Palczewski, Krzysztof, Engel, Andreas
G protein-coupled receptors (GPCRs), which constitute the largest and structurally best conserved family of signaling molecules, are involved in virtually all physiological processes. Crystal...
The G protein-coupled receptor rhodopsin in the native membrane
Fotiadis, Dimitrios, Liang, Yan, Filipek, Slawomir, Saperstein, David A., Engel, Andreas, Palczewski, Krzysztof
The higher-order structure of G protein-coupled receptors (GPCRs) in membranes may involve dimerization and formation of even larger oligomeric complexes. Here, we have investigated the organization...
Molecular architecture of photosynthetic membranes in Rhodobacter sphaeroides: the role of PufX
Siebert, C Alistair, Qian, Pu, Fotiadis, Dimitrios, Engel, Andreas, Hunter, C Neil, Bullough, Per A
The effects of the PufX polypeptide on membrane architecture were investigated by comparing the composition and structures of photosynthetic membranes from PufX+ and PufX− strains of Rhodobacter...
Bacterial Na+-ATP synthase has an undecameric rotor
Stahlberg, Henning, Müller, Daniel J., Suda, Kitaru, Fotiadis, Dimitrios, Engel, Andreas, Meier, Thomas, ...
Synthesis of adenosine triphosphate (ATP) by the F1F0 ATP synthase involves a membrane-embedded rotary engine, the F0 domain, which drives the extra-membranous catalytic F1 domain. The F0 domain...
Electron and atomic force microscopy of the trimeric ammonium transporter AmtB
Conroy, Matthew J, Jamieson, Stuart J, Blakey, Daniel, Kaufmann, Thomas, Engel, Andreas, Fotiadis, Dimitrios, ...
Escherichia coli AmtB is an archetypal member of the ammonium transporter (Amt) family, a family of proteins that are conserved in all domains of life. Reconstitution of AmtB in the presence of...
Geiser, Andrew H., Sievert, Michael K., Guo, Lian-Wang, Grant, Jennifer E., Krebs, Mark P., Fotiadis, Dimitrios, ...
The mechanisms by which G-protein-coupled receptors (GPCRs) activate G-proteins are not well understood due to the lack of atomic structures of GPCRs in an active form or in GPCR/G-protein complexes....