Optogenetic dissection of a behavioural module in the vertebrate spinal cord (2009)
Wyart, Claire, Bene, Filippo Del, Warp, Erica, Scott, Ethan K., Trauner, Dirk, Baier, Herwig, ...
Locomotion relies on neural networks called central pattern generators (CPGs) that generate periodic motor commands for rhythmic movements1. In vertebrates, the excitatory synaptic drive for inducing...
Optogenetic dissection of a behavioural module in the vertebrate spinal cord (2009)
Wyart, Claire, Bene, Filippo Del, Warp, Erica, Scott, Ethan K., Trauner, Dirk, Baier, Herwig, ...
Locomotion relies on neural networks called central pattern generators (CPGs) that generate periodic motor commands for rhythmic movements1. In vertebrates, the excitatory synaptic drive for inducing...
Optogenetic dissection of a behavioural module in the vertebrate spinal cord (2009)
Wyart, Claire, Bene, Filippo Del, Warp, Erica, Scott, Ethan K., Trauner, Dirk, Baier, Herwig, ...
Locomotion relies on neural networks called central pattern generators (CPGs) that generate periodic motor commands for rhythmic movements1. In vertebrates, the excitatory synaptic drive for inducing...
A structural link between inactivation and block of a K+ channel (2008)
Christian Ader, Robert Schneider, Sönke Hornig, Phanindra Velisetty, Erica M Wilson, Adam Lange, ...
Photochemical control of endogenous ion channels and cellular excitability (2008)
Doris L Fortin, Matthew R Banghart, Timothy W Dunn, Katharine Borges, Daniel A Wagenaar, Quentin Gaudry, ...
Synthesis of Taiwaniaquinoids via Nazarov Triflation (2008)
Guangxin Liang, Yue Xu, Ian B. Seiple, Dirk Trauner
The taiwaniaquinoids are a family of unusual tricyclic diterpenoids isolated from East Asian conifers with interesting biological activities (Chart 1). 1 Structurally, their members are marked by a...
Reversibly Caged Glutamate: A Photochromic Agonist of Ionotropic (2008)
Glutamate Receptors, Matthew Volgraf, Pau Gorostiza, Stephanie Szobota, Max R. Helix, Ehud Y. Isacoff, ...
The ability to control the active concentration of neurotransmitters in a spatially and temporally precise manner has revolutionized the study of the central nervous system (CNS). In particular,...
Total Synthesis of (-)-Archazolid B (2008)
Paul A. Roethle, Ingrid T. Chen, Dirk Trauner
The archazolids are a family of unsaturated polyketides with low nanomolar inhibitory activity and excellent selectivity against mammalian V-ATPases (Scheme 1). 1 The isolation of archazolids...
Remote Control of Neuronal Activity with a Light-Gated Glutamate Receptor (2008)
Stephanie Szobota, Pau Gorostiza, Claire Wyart, Doris L. Fortin, Kathleen D. Kolstad, Orapim Tulyathan, ...
The ability to stimulate select neurons in isolated tissue and in living animals is important for investigating their role in circuits and behavior. We show that the engineered light-gated ionotropic...
Dienophile Twisting and Substituent Effects Influence Reaction Rates of (2008)
Intramolecular Diels-Alder Cycloadditions, Kelli S. Khuong, Chris M. Beaudry, Dirk Trauner, K. N. Houk
e TS is reminiscent of that observed for the intermolecular cycloadditions involving cyclopentadiene. What distinguishes this intramolecular cycloaddition from typical cycloadditions is that...
Biomimetic Synthesis of (()-Pinnatal and (()-Sterekunthal A (2008)
Jeremiah Malerich And, Jeremiah P. Malerich, Dirk Trauner
t is interesting to speculate whether all the natural products shown in Chart 1 are derived from a common biosynthetic precursor, naphthoquinone 8, through a series of oxidations and pericyclic...
Biomimetic Synthesis and Structure Elucidation of Rubicordifolin, a Cytotoxic (2008)
Natural Product From, Jean-philip Lumb, Dirk Trauner
ne 4 is a known natural product previously isolated from various Rubiaceae. 6 Oxidation with concomitant allylic transposition would afford the reactive vinyl quinone 5 as a hypothetical biosynthetic...
Enantioselective Nazarov Reactions through Catalytic Asymmetric Proton (2008)
Transfer Guangxin Liang, Guangxin Liang, Dirk Trauner
f 20 mol % of the chiral scandium triflate pybox complex 8 (Scheme 2, eq 1). The product 7 was formed in 53% yield and 61% enantiomeric excess (ee). Concomitant with our report, Aggarwal et al....
Biosynthetic and Biomimetic Electrocyclizations (2008)
Christopher Beaudry Jeremiah, Biomimetic Electrocyclizations, Christopher M. Beaudry, Jeremiah P. Malerich, Dirk Trauner
Contents 1. Introduction 4757 2. All-Carbon Systems 4758 2.1. 4# Systems 4758 2.2. 6# Systems 4759 2.3. 8# Systems 4763 3. Heteroatom-Containing Systems 4766 3.1. Oxa-6# Systems 4766 3.2. Aza-6#...
Remote control of neuronal activity with a light-gated glutamate receptor (2007)
Szobota, Stephanie, Gorostiza, Pau, Del Bene, Filippo, Wyart, Claire, Fortin, Doris L., Kolstad, Kathleen D., ...
The ability to stimulate select neurons in isolated tissue and in living animals is important for investigating their role in circuits and behavior. We show that the engineered light-gated ionotropic...
Remote control of neuronal activity with a light-gated glutamate receptor (2007)
Szobota, Stephanie, Gorostiza, Pau, Del Bene, Filippo, Wyart, Claire, Fortin, Doris L., Kolstad, Kathleen D., ...
The ability to stimulate select neurons in isolated tissue and in living animals is important for investigating their role in circuits and behavior. We show that the engineered light-gated ionotropic...
All Optical Interface for Parallel, Remote, and Spatiotemporal Control of Neuronal Activity (2007)
Sheng Wang, Stephanie Szobota, Yuan Wang, Matthew Volgraf, Zhaowei Liu, Cheng Sun, ...
A key technical barrier to furthering our understanding of complex neural networks has been the lack of tools for the simultaneous spatiotemporal control and detection of activity in a large number...
Remote control of neuronal activity with a light-gated glutamate receptor (2007)
Szobota, Stephanie, Gorostiza, Pau, Del Bene, Filippo, Wyart, Claire, Fortin, Doris L., Kolstad, Kathleen D., ...
The ability to stimulate select neurons in isolated tissue and in living animals is important for investigating their role in circuits and behavior. We show that the engineered light-gated ionotropic...
Remote control of neuronal activity with a light-gated glutamate receptor (2007)
Szobota, Stephanie, Gorostiza, Pau, Del Bene, Filippo, Wyart, Claire, Fortin, Doris L., Kolstad, Kathleen D., ...
The ability to stimulate select neurons in isolated tissue and in living animals is important for investigating their role in circuits and behavior. We show that the engineered light-gated ionotropic...
Reversible Photomechanical Switching of Individual Engineered Molecules at a Surface (2006)
Comstock, Matthew J., Levy, Niv, Kirakosian, Armen, Cho, Jongweon, Lauterwasser, Frank, Harvey, Jessica H., ...
We have observed reversible light-induced mechanical switching for a single organic molecule bound to a metal surface. Scanning tunneling microscopy (STM) was used to image the features of an...
Engineering Light-Gated Ion Channels † (2006)
Matthew R. Banghart, Matthew Volgraf, Dirk Trauner
ABSTRACT: Ion channels are gated by a variety of stimuli, including ligands, voltage, membrane tension, temperature, and even light. Natural gates can be altered and augmented using synthetic...
Infrared Hole Burning and Conformational Change in a Borane-Ammonia Complex (2005)
Christopher Endicott Herbert, Herbert L. Strauss, Chambers C. Hughes, Dirk Trauner
Introduction We have recently described a novel series of boraneammonia complexes in which the ammonia is bonded to a substituted boron atom and the three hydrogen atoms are each hydrogen bonded to...
Concise Synthesis Of, Dirk Trauner
The total synthesis of smenochromene D, an unusual ansa-terpenoid is described. The synthesis features an unprecedented macrocyclization proceeding through a biomimetic electrocyclization. The...
Development of novel Lewis acid catalyzed cycloisomerizations: (2003)
Synthesis Of Bicyclo, Aubry K. Miller, Matthew R. Banghart, Christopher M. Beaudry, Judy M. Suh, Dirk Trauner
The Lewis acid catalyzed cyclization of hexatrienes and pentadienals to bicyclo[3.1.0]hexenes and cyclopentenones, respectively, was investigated. The application of the former reaction to the total...
Protein Surface Recognition by Rational Design: Nanomolar Ligands for (2003)
Potassium Channels Stefan, Stefan N. Gradl, John P. Felix, Ehud Y. Isacoff, Maria L. Garcia, Dirk Trauner
ys involving radioactive 86 Rb ions. 8 Electrophysiological methods can also be used to investigate the blocking characteristics of ligands, either at the whole cell (voltage clamp) or single channel...
Research By Bgevig, K. Juhl, N. Kumaragurubaran, W. Zhuang, K. A. Jørgensen, Commentary Chambers C. Hughes, ...
ORGANIC CHEMISTRY 16:511 -- 517 (2003) # 2003 Data Trace Publishing Company CCC 1431-9268 O O O O H O Me OH O O OH NH 2 OMe (PMPNH 2 ) O Ar H O OH Ar NHPMP O OH O Me O DMF, rt 100% 93% ee DMSO 97%...
Imprimatur Date Signature, Unsaturated Morpholine Amides, A Stefan, Practical Variant, N. Gradl, Joshua J. Kennedy-smith, ...
A Claisen--Eschenmoser type rearrangement giving rise to unsaturated morpholine-, piperidine- and pyrrolidine amides is described.
The total synthesis of (–)-crispatene
Miller, Aubry K., Byun, Daniel H., Beaudry, Christopher M., Trauner, Dirk
The total synthesis of the molluscan polypropionate (–)-crispatene is described. The synthesis features a palladium-catalyzed cross-coupling to establish a sensitive conjugated tetraene and its...
Light-activated ion channels for remote control of neuronal firing
Banghart, Matthew, Borges, Katharine, Isacoff, Ehud, Trauner, Dirk, Kramer, Richard H
Neurons have ion channels that are directly gated by voltage, ligands and temperature but not by light. Using structure-based design, we have developed a new chemical gate that confers light...
Allosteric control of an ionotropic glutamate receptor with an optical switch
Volgraf, Matthew, Gorostiza, Pau, Numano, Rika, Kramer, Richard H, Isacoff, Ehud Y, Trauner, Dirk
The precise regulation of protein activity is fundamental to life. The allosteric control of an active site by a remote regulatory binding site is a mechanism of regulation found across protein...
Protein Surface Recognition by Rational Design: Nanomolar Ligands for Potassium Channels
Gradl, Stefan N., Felix, John P., Isacoff, Ehud Y., Garcia, Maria L., Trauner, Dirk
The total synthesis of (–)-crispatene
Miller, Aubry K., Byun, Daniel H., Beaudry, Christopher M., Trauner, Dirk
The total synthesis of the molluscan polypropionate (–)-crispatene is described. The synthesis features a palladium-catalyzed cross-coupling to establish a sensitive conjugated tetraene and its...
Mechanisms of photoswitch conjugation and light activation of an ionotropic glutamate receptor
Gorostiza, Pau, Volgraf, Matthew, Numano, Rika, Szobota, Stephanie, Trauner, Dirk, Isacoff, Ehud Y.
The analysis of cell signaling requires the rapid and selective manipulation of protein function. We have synthesized photoswitches that covalently modify target proteins and reversibly present and...
Nanosculpting reversed wavelength sensitivity into a photoswitchable iGluR
Numano, Rika, Szobota, Stephanie, Lau, Albert Y., Gorostiza, Pau, Volgraf, Matthew, Roux, Benoit, ...
Photoswitched tethered ligands (PTLs) can be used to remotely control protein function with light. We have studied the geometric and conformational factors that determine the efficacy of PTL gating...
Calix[4]arene-based conical-shaped ligands for voltage-dependent potassium channels
Martos, Vera, Bell, Sarah C., Santos, Eva, Isacoff, Ehud Y., Trauner, Dirk, De Mendoza, Javier
Potassium channels are among the core functional elements of life because they underpin essential cellular functions including excitability, homeostasis, and secretion. We present here a series of...
A Signal Processing Analysis of Purkinje Cells in vitro
Abrams, Ze'ev R., Warrier, Ajithkumar, Trauner, Dirk, Zhang, Xiang
Cerebellar Purkinje cells in vitro fire recurrent sequences of Sodium and Calcium spikes. Here, we analyze the Purkinje cell using harmonic analysis, and our experiments reveal that its output signal...