Whiley, DM, Limnios, EA, Ray, S, Sloots, TP, Tapsall, JW
Increasing numbers of Neisseria gonorrhoeae strains with decreased susceptibilities to ceftriaxone and other oral cephalosporins widely used for the treatment of gonorrhea have been isolated in...
Bialasiewicza, S, Whiley, DM, Lambert, SB, Gould, A, Nissen, MD, Sloots, TP
Background: Recently, novel human polyomaviruses, KI (KIV) and WU (WUV) were described. Their role in human disease has not yet been determined. Objectives: The aim of this study was to develop...
Bialasiewicza, S, Whiley, DM, Lambert, SB, Wang, D, Nissen, MD, Sloot, TP
Background: Recently, Allander and co-workers reported the discovery of a new human polyomavirus, KI virus, in respiratory secretions from patients with acute respiratory tract infection (ARTI)....
Impact of competitive inhibition and sequence variation upon the sensitivity of malaria PCR (2007)
Bialasiewicz, S, Whiley, DM, Nissen, MD, Sloots, TP
Real-time PCR assays for Plasmodium species utilizing universal and species-specific primers were compared to investigate variables influencing decreased assay sensitivity. Sequence variation in...
Nucleic acid amplification testing for Neisseria gonorrhoeae - An ongoing challenge (2006)
Whiley, DM, Tapsall, JW, Sloots, TP
Nucleic acid amplification tests (NAATs) for the detection of Neisseria gonorrhoeae became available in the early 1990s. Although offering several advantages over traditional detection methods, N....
A minor groove binder (MGB) TaqMan real-time PCR assay was developed for the detection of respiratory syncytial virus (RSV) in clinical specimens. Upon evaluation of the assay, notable differences...
Tapsall, JW, Limnios, EA, Nguyen, NL, Carter, I, Lum, G, Freeman, K, ...
Aims: To develop and evaluate multiplex PCR assays for Chlamydia trachomatis and Neisseria gonorrhoeae, using real-time and conventional PCR detection methodologies. Methods: Two real-time multiplex...
A 5'-nuclease real-time reverse transcriptase-polymerase chain reaction assay was developed for the detection of influenza type A and was validated using a range of influenza A subtypes, including...
Whiley, DM, Mackay, IM, Syrmis, MW, Witt, MJ, Sloots, TP
Background: In recent years polymerase chain reaction (PCR) has proven to be a highly sensitive and specific method for the diagnosis of herpes simplex virus (HSV) infections. The advent of real-time...
Whiley, DM, Arden, KE, Mackay, IM, Syrmis, MW, Sloots, TP
Human polyomaviruses JCV and BKV can cause several clinical manifestations in immunocompromised hosts, including progressive multifocal leukoencephalopathy (PML) and haemorrhagic cystitis. Molecular...
Whiley, DM, LeCornec, GM, Baddeley, A, Savill, J, Syrmis, MW, Mackay, IM, ...
Polymerase chain reaction (PCR) is now recognized as a sensitive and specific method for detecting Plasmodium species in blood. In this Study. we tested 279 blood samples, from patients with...
Detection and differentiation of human polyomaviruses JC and BK by LightCycler PCR
Whiley, DM, Mackay, IM, Sloots, TP
Human polyomaviruses JC and BK may cause several clinical manifestations in immunocompromised hosts, including progressive multifocal leukoencephalopathy and hemorrhagic cystitis. Molecular detection...
A real-time PCR assay for the detection of Neisseria gonorrhoeae by LightCycler
Whiley, DM, LeCornec, GM, Mackay, IM, Siebert, DJ, Sloots, TP
The detection of Neisseria gonorrhoeae by the polymerase chain reaction (PCR) is now recognized as a sensitive and specific method of diagnosing infection by the organism. In this Study 152 urine...
Whiley, DM, Syrmis, MW, Mackay, IM, Sloots, TP
Laboratory diagnosis of human respiratory syncytial virus (hRSV) infections has traditionally been performed by virus isolation in cell culture and the direct fluorescent-antibody assay (DFA)....
Molecular assays for detection of human metapneumovirus
Mackay, IM, Jacob, KC, Woolhouse, D, Waller, K, Syrmis, MW, Whiley, DM, ...
The recent description of the respiratory pathogen human metapneumovirus (hMPV) has highlighted a deficiency in current diagnostic techniques for viral agents associated with acute lower respiratory...
Detection of Neisseria meningitidis by LightCycler PCR
Whiley, DM, Crisante, ME, Syrmis, MW, Mackay, IM, Sloots, TP
Whiley, DM, Syrmis, MW, Mackay, IM, Sloots, TP
Three Herpes Simplex Virus LightCycler polymerase chain reaction assays were compared for the detection of herpes simplex virus in 48 swab specimens. The assays comprised of one in-house assay and...
Co-detection and discrimination of six human herpesviruses by multiplex PCR-ELAHA
Mackay, IM, Gardam, T, Arden, KE, McHardy, S, Whiley, DM, Crisante, E, ...
Background: Herpesviruses are a significant cause of human morbidity. Traditional approaches to the identification of these viruses require infectious or at least antigenic virus. Multiplex PCR...
Whiley, DM, Arden, KE, Mackay, IM, Syrmis, MW, Sloots, TP
Human polyomaviruses JCV and BKV can cause several clinical manifestations in immunocompromised hosts, including progressive multifocal leukoencephalopathy (PML) and haemorrhagic cystitis. Molecular...
Syrmis, MW, Whiley, DM, Thomas, M, Mackay, IM, Williamson, J, Siebert, DJ, ...
Cell culture and direct fluorescent antibody (DFA) assays have been traditionally used for the laboratory diagnosis of respiratory viral infections. Multiplex reverse transcriptase polymerase chain...
Whiley, DM, Mackay, IM, Syrmis, MW, Witt, MJ, Sloots, TP
Background: In recent years polymerase chain reaction (PCR) has proven to be a highly sensitive and specific method for the diagnosis of herpes simplex virus (HSV) infections. The advent of real-time...
Whiley, DM, LeCornec, GM, Baddeley, A, Savill, J, Syrmis, MW, Mackay, IM, ...
Polymerase chain reaction (PCR) is now recognized as a sensitive and specific method for detecting Plasmodium species in blood. In this Study. we tested 279 blood samples, from patients with...