Pennartz, Anne, Genereux, Catherine, Parquet, Claudine, Mengin-Lecreulx, Dominique, Joris, Bernard
In the eubacterial cell, the peptidoglycan is perpetually hydrolyzed throughout the cell cycle by different enzymes such as lytic transglycosylases, endopeptidases, and amidases. In Escherichia coli,...
Magnet, Sophie, Bellais, Samuel, Dubost, Lionel, Fourgeaud, Martine, Mainardi, Jean-Luc, Petit-Frère, Sébastien, ...
The L,D-transpeptidase Ldt(fm) catalyzes peptidoglycan cross-linking in beta-lactam-resistant mutant strains of Enterococcus faecium. Here, we show that in Escherichia coli Ldt(fm) homologues are...
Magnet, Sophie, Bellais, Samuel, Dubost, Lionel, Fourgeaud, Martine, Mainardi, Jean-Luc, Petit-Frère, Sébastien, ...
The L,D-transpeptidase Ldt(fm) catalyzes peptidoglycan cross-linking in beta-lactam-resistant mutant strains of Enterococcus faecium. Here, we show that in Escherichia coli Ldt(fm) homologues are...
Magnet, Sophie, Bellais, Samuel, Dubost, Lionel, Fourgeaud, Martine, Mainardi, Jean-Luc, Petit-Frère, Sébastien, ...
The L,D-transpeptidase Ldt(fm) catalyzes peptidoglycan cross-linking in beta-lactam-resistant mutant strains of Enterococcus faecium. Here, we show that in Escherichia coli Ldt(fm) homologues are...
Role of AmiA in the Morphological Transition of Helicobacter pylori and in Immune Escape (2006)
Catherine Chaput, Chantal Ecobichon, Nadège Cayet, Stephen E. Girardin, Catherine Werts, Stéphanie Guadagnini, ...
The human gastric pathogen Helicobacter pylori is responsible for peptic ulcers and neoplasia. Both in vitro and in the human stomach it can be found in two forms, the bacillary and coccoid forms....
Fraipont, Claudine, Sapunaric, Frédéric, Zervosen, Astrid, Auger, Geneviève, Devreese, Bart, Lioux, Thierry, ...
The glycosyl transferase of the Escherichia coli bifunctional penicillin-binding protein (PBP) 1b catalyzes the assembly of lipid-transported...
Role of AmiA in the morphological transition of Helicobacter pylori and in immune escape. (2006)
Chaput, Catherine, Ecobichon, Chantal, Cayet, Nadège, Girardin, Stephen E, Werts, Catherine, Guadagnini, Stéphanie, ...
The human gastric pathogen Helicobacter pylori is responsible for peptic ulcers and neoplasia. Both in vitro and in the human stomach it can be found in two forms, the bacillary and coccoid forms....
Role of AmiA in the morphological transition of Helicobacter pylori and in immune escape. (2006)
Chaput, Catherine, Ecobichon, Chantal, Cayet, Nadège, Girardin, Stephen E, Werts, Catherine, Guadagnini, Stéphanie, ...
The human gastric pathogen Helicobacter pylori is responsible for peptic ulcers and neoplasia. Both in vitro and in the human stomach it can be found in two forms, the bacillary and coccoid forms....
Role of AmiA in the morphological transition of Helicobacter pylori and in immune escape. (2006)
Chaput, Catherine, Ecobichon, Chantal, Cayet, Nadège, Girardin, Stephen E, Werts, Catherine, Guadagnini, Stéphanie, ...
The human gastric pathogen Helicobacter pylori is responsible for peptic ulcers and neoplasia. Both in vitro and in the human stomach it can be found in two forms, the bacillary and coccoid forms....
Chung-I Chang, Sébastien Pili-Floury, Mireille Hervé, Claudine Parquet, Yogarany Chelliah, Bruno Lemaitre, ...
The Drosophila protein PGRP-SA is found to have an intrinsic activity to cleave L,D-configured peptide bonds, which exist only in prokaryotes. This work reveals a rare enzymatic activity in a...
Chung-I Chang, Sébastien Pili-Floury, Mireille Hervé, Claudine Parquet, Yogarany Chelliah, Bruno Lemaitre, ...
The Drosophila peptidoglycan recognition protein SA (PGRP-SA) is critically involved in sensing bacterial infection and activating the Toll signaling pathway, which induces the expression of specific...
Nucleotide sequence of the cell-envelope murG gene of Escherichia coli (1990)
Mengin-Lecreulx, Dominique, Texier, Laurent, Heijenoort, Jean Van
Autophosphorylation of Phosphoglucosamine Mutase from Escherichia coli
Jolly, Laure, Pompeo, Frédérique, Van Heijenoort, Jean, Fassy, Florence, Mengin-Lecreulx, Dominique
Phosphoglucosamine mutase (GlmM) catalyzes the formation of glucosamine-1-phosphate from glucosamine-6-phosphate, an essential step in the pathway for UDP-N-acetylglucosamine biosynthesis in...
Identification of the Pseudomonas aeruginosa glmM Gene, Encoding Phosphoglucosamine Mutase
Tavares, Isabel M., Jolly, Laure, Pompeo, Frédérique, Leitão, Jorge H., Fialho, Arsénio M., Sá-Correia, Isabel, ...
A search for a potential algC homologue within the Pseudomonas aeruginosa PAO1 genome database has revealed an open reading frame (ORF) of unknown function, ORF540 in contig 54 (July 1999 Pseudomonas...
Bouhss, Ahmed, Josseaume, Nathalie, Allanic, David, Crouvoisier, Muriel, Gutmann, Laurent, Mainardi, Jean-Luc, ...
Many species of gram-positive bacteria produce branched peptidoglycan precursors resulting from the transfer of various l-amino acids or glycine from amino acyl-tRNA to the ɛ-amino group of...
Mengin-Lecreulx, Dominique, Falla, Tim, Blanot, Didier, Van Heijenoort, Jean, Adams, David J., Chopra, Ian
The monomer units in the Escherichia coli and Staphylococcus aureus cell wall peptidoglycans differ in the nature of the third amino acid in the l-alanyl-γ-d-glutamyl-X-d-alanyl-d-alanine side...
Park, James T., Raychaudhuri, Debabrata, Li, Hongshan, Normark, Staffan, Mengin-Lecreulx, Dominique
Mutants of a diaminopimelic acid (Dap)-requiring strain of Escherichia coli were isolated which failed to grow on media in which Dap was replaced by the cell wall murein tripeptide,...
Mengin-Lecreulx, Dominique, Ayala, Juan, Bouhss, Ahmed, Van Heijenoort, Jean, Parquet, Claudine, Hara, Hiroshi
Recently, a promoter for the essential gene ftsI, which encodes penicillin-binding protein 3 of Escherichia coli, was precisely localized 1.9 kb upstream from this gene, at the beginning of the mra...
Pompeo, Frédérique, Van Heijenoort, Jean, Mengin-Lecreulx, Dominique
The glucosamine-1-phosphate acetyltransferase activity but not the uridyltransferase activity of the bifunctional GlmU enzyme from Escherichia coli was lost when GlmU was stored in the absence of...
Hesse, Lars, Bostock, Julieanne, Dementin, Sebastien, Blanot, Didier, Mengin-Lecreulx, Dominique, Chopra, Ian
Chlamydiae are unusual obligate intracellular bacteria that cause serious infections in humans. Chlamydiae contain genes that appear to encode products with peptidoglycan biosynthetic activity. The...
Arbeloa, Ana, Segal, Heidi, Hugonnet, Jean-Emmanuel, Josseaume, Nathalie, Dubost, Lionnel, Brouard, Jean-Paul, ...
Peptidoglycan polymerization complexes contain multimodular penicillin-binding proteins (PBP) of classes A and B that associate a conserved C-terminal transpeptidase module to an N-terminal...
Fluorescence Detection-Based Functional Assay for High-Throughput Screening for MraY
Stachyra, Thérèse, Dini, Christophe, Ferrari, Paul, Bouhss, Ahmed, Van Heijenoort, Jean, Mengin-Lecreulx, Dominique, ...
We have developed a novel assay specific to MraY, which catalyzes the first membrane step in the biosynthesis of bacterial cell wall peptidoglycan. This was accomplished by using...
Chang, Chung-I, Pili-Floury, Sébastien, Hervé, Mireille, Parquet, Claudine, Chelliah, Yogarany, Lemaitre, Bruno, ...
The Drosophila peptidoglycan recognition protein SA (PGRP-SA) is critically involved in sensing bacterial infection and activating the Toll signaling pathway, which induces the expression of specific...
Chang, Chung-I, Ihara, Kentaro, Chelliah, Yogarany, Mengin-Lecreulx, Dominique, Wakatsuki, Soichi, Deisenhofer, Johann
The peptidoglycan-recognition protein LCa (PGRP-LCa) is a transmembrane receptor required for activation of the Drosophila immune deficiency pathway by monomeric Gram-negative peptidoglycan. We have...
Role of AmiA in the Morphological Transition of Helicobacter pylori and in Immune Escape
Chaput, Catherine, Ecobichon, Chantal, Cayet, Nadège, Girardin, Stephen E, Werts, Catherine, Guadagnini, Stéphanie, ...
The human gastric pathogen Helicobacter pylori is responsible for peptic ulcers and neoplasia. Both in vitro and in the human stomach it can be found in two forms, the bacillary and coccoid forms....
Murine Nod1 but not its human orthologue mediates innate immune detection of tracheal cytotoxin
Magalhaes, Joao Gamelas, Philpott, Dana J, Nahori, Marie-Anne, Jéhanno, Muguette, Fritz, Joerg, Bourhis, Lionel Le, ...
Tracheal cytotoxin (TCT) was originally described as the minimal effector that was able to reproduce the cytotoxic response of Bordetella pertussis on ciliated epithelial cells. This molecule...
Autophosphorylation of Phosphoglucosamine Mutase from Escherichia coli
Jolly, Laure, Pompeo, Frédérique, Van Heijenoort, Jean, Fassy, Florence, Mengin-Lecreulx, Dominique
Phosphoglucosamine mutase (GlmM) catalyzes the formation of glucosamine-1-phosphate from glucosamine-6-phosphate, an essential step in the pathway for UDP-N-acetylglucosamine biosynthesis in...
Identification of the Pseudomonas aeruginosa glmM Gene, Encoding Phosphoglucosamine Mutase
Tavares, Isabel M., Jolly, Laure, Pompeo, Frédérique, Leitão, Jorge H., Fialho, Arsénio M., Sá-Correia, Isabel, ...
A search for a potential algC homologue within the Pseudomonas aeruginosa PAO1 genome database has revealed an open reading frame (ORF) of unknown function, ORF540 in contig 54 (July 1999 Pseudomonas...
Bouhss, Ahmed, Josseaume, Nathalie, Allanic, David, Crouvoisier, Muriel, Gutmann, Laurent, Mainardi, Jean-Luc, ...
Many species of gram-positive bacteria produce branched peptidoglycan precursors resulting from the transfer of various l-amino acids or glycine from amino acyl-tRNA to the ɛ-amino group of...
Mengin-Lecreulx, Dominique, Falla, Tim, Blanot, Didier, Van Heijenoort, Jean, Adams, David J., Chopra, Ian
The monomer units in the Escherichia coli and Staphylococcus aureus cell wall peptidoglycans differ in the nature of the third amino acid in the l-alanyl-γ-d-glutamyl-X-d-alanyl-d-alanine side...
Park, James T., Raychaudhuri, Debabrata, Li, Hongshan, Normark, Staffan, Mengin-Lecreulx, Dominique
Mutants of a diaminopimelic acid (Dap)-requiring strain of Escherichia coli were isolated which failed to grow on media in which Dap was replaced by the cell wall murein tripeptide,...
Mengin-Lecreulx, Dominique, Ayala, Juan, Bouhss, Ahmed, Van Heijenoort, Jean, Parquet, Claudine, Hara, Hiroshi
Recently, a promoter for the essential gene ftsI, which encodes penicillin-binding protein 3 of Escherichia coli, was precisely localized 1.9 kb upstream from this gene, at the beginning of the mra...
Pompeo, Frédérique, Van Heijenoort, Jean, Mengin-Lecreulx, Dominique
The glucosamine-1-phosphate acetyltransferase activity but not the uridyltransferase activity of the bifunctional GlmU enzyme from Escherichia coli was lost when GlmU was stored in the absence of...
Hesse, Lars, Bostock, Julieanne, Dementin, Sebastien, Blanot, Didier, Mengin-Lecreulx, Dominique, Chopra, Ian
Chlamydiae are unusual obligate intracellular bacteria that cause serious infections in humans. Chlamydiae contain genes that appear to encode products with peptidoglycan biosynthetic activity. The...
Arbeloa, Ana, Segal, Heidi, Hugonnet, Jean-Emmanuel, Josseaume, Nathalie, Dubost, Lionnel, Brouard, Jean-Paul, ...
Peptidoglycan polymerization complexes contain multimodular penicillin-binding proteins (PBP) of classes A and B that associate a conserved C-terminal transpeptidase module to an N-terminal...
Fluorescence Detection-Based Functional Assay for High-Throughput Screening for MraY
Stachyra, Thérèse, Dini, Christophe, Ferrari, Paul, Bouhss, Ahmed, Van Heijenoort, Jean, Mengin-Lecreulx, Dominique, ...
We have developed a novel assay specific to MraY, which catalyzes the first membrane step in the biosynthesis of bacterial cell wall peptidoglycan. This was accomplished by using...
Chang, Chung-I, Pili-Floury, Sébastien, Hervé, Mireille, Parquet, Claudine, Chelliah, Yogarany, Lemaitre, Bruno, ...
The Drosophila peptidoglycan recognition protein SA (PGRP-SA) is critically involved in sensing bacterial infection and activating the Toll signaling pathway, which induces the expression of specific...
Chang, Chung-I, Ihara, Kentaro, Chelliah, Yogarany, Mengin-Lecreulx, Dominique, Wakatsuki, Soichi, Deisenhofer, Johann
The peptidoglycan-recognition protein LCa (PGRP-LCa) is a transmembrane receptor required for activation of the Drosophila immune deficiency pathway by monomeric Gram-negative peptidoglycan. We have...
Murine Nod1 but not its human orthologue mediates innate immune detection of tracheal cytotoxin
Magalhaes, Joao Gamelas, Philpott, Dana J, Nahori, Marie-Anne, Jéhanno, Muguette, Fritz, Joerg, Bourhis, Lionel Le, ...
Tracheal cytotoxin (TCT) was originally described as the minimal effector that was able to reproduce the cytotoxic response of Bordetella pertussis on ciliated epithelial cells. This molecule...
Role of AmiA in the Morphological Transition of Helicobacter pylori and in Immune Escape
Chaput, Catherine, Ecobichon, Chantal, Cayet, Nadège, Girardin, Stephen E, Werts, Catherine, Guadagnini, Stéphanie, ...
The human gastric pathogen Helicobacter pylori is responsible for peptic ulcers and neoplasia. Both in vitro and in the human stomach it can be found in two forms, the bacillary and coccoid forms....
Magnet, Sophie, Bellais, Samuel, Dubost, Lionel, Fourgeaud, Martine, Mainardi, Jean-Luc, Petit-Frère, Sébastien, ...
The l,d-transpeptidase Ldtfm catalyzes peptidoglycan cross-linking in β-lactam-resistant mutant strains of Enterococcus faecium. Here, we show that in Escherichia coli Ldtfm homologues are...
Hervé, Mireille, Boniface, Audrey, Gobec, Stanislav, Blanot, Didier, Mengin-Lecreulx, Dominique
The UDP-N-acetylmuramate:l-alanyl-γ-d-glutamyl-meso-diaminopimelate ligase (murein peptide ligase [Mpl]) is known to be a recycling enzyme allowing reincorporation into peptidoglycan (murein) of the...
Mohammadi, Tamimount, Karczmarek, Aneta, Crouvoisier, Muriel, Bouhss, Ahmed, Mengin-Lecreulx, Dominique, Den Blaauwen, Tanneke
In Escherichia coli many enzymes including MurG are directly involved in the synthesis and assembly of peptidoglycan. MurG is an essential glycosyltransferase catalysing the last intracellular step...
Periplasmic phosphorylation of lipid A is linked to the synthesis of undecaprenyl phosphate
Touzé, Thierry, Tran, An X, Hankins, Jessica V, Mengin-Lecreulx, Dominique, Trent, M Stephen
One-third of the lipid A found in the Escherichia coli outer membrane contains an unsubstituted diphosphate unit at position 1 (lipid A 1-diphosphate). We now report an inner membrane enzyme, LpxT...
Al-Dabbagh, Bayan, Mengin-Lecreulx, Dominique, Bouhss, Ahmed
To date, the structural and functional characterization of proteins belonging to the polyprenyl-phosphate N-acetylhexosamine-1-phosphate transferase superfamily has been relentlessly held back by...