Noble metals strip peptides from class II MHC proteins (2006)
De Wall, Stephen J., Painter, Corrie A., Stone, Jennifer D., Bandaranayake, Rajintha, Wiley, Don C., Mitchison, Timothy J., ...
Class II major histocompatibility complex (MHC) proteins are essential for normal immune system function but also drive many autoimmune responses. They bind peptide antigens in endosomes and present...
Stratikos, Efstratios, Wiley, Don C., Stern, Lawrence J.
The class II MHC homolog HLA-DM catalyzes exchange of peptides bound to class II MHC proteins, and is an important component of the Ag presentation machinery. The mechanism of HLA-DM-mediated...
Structural Analysis of the Human T-Cell Receptor/HLA-A2/Peptide Complex. (1998)
Wiley, Don C., Garboczi, David N.
During the first year of the grant, we obtained sufficient amounts of the T-cell receptor (TCR) for crystallization experiments. This was achieved by protein engineering and by protein refolding...
Structural Analysis of the Human T-Cell Receptor/HLA-A2/Peptide Complex. (1998)
Wiley, Don C., Garboczi, David N.
In this final report, we describe our efforts that culminated in the determination of the x-ray crystal structure of a T cell receptor-MHC molecule (TCR/MHC) complex. Based on the structure, we...
Structural Analysis of the Human T-Cell Receptor/HLA-A2/Peptide Complex. (1997)
Wiley, Don C., Garboczi, David N.
During the second year of this grant, we obtained better X-ray data from the crystals of the T-cell receptor/HLA-A2/peptide complex, by finding improved conditions for freezing the crystals, by...
Michael B. Eisen, Subramaniam Sabesan, John J. Skehel, Don C. Wiley
The structures of five complexes of the X-31 influenza A (H3N2) virus hemagglutinin with sialyloligosaccharide receptor analogs have been determined from 2.5 to 2.8 A ˚ resolution by X-ray...
Chen, Jue, Skehel, John J., Wiley, Don C.
The structure of a stable recombinant ectodomain of influenza hemagglutinin HA2 subunit, EHA2 (23–185), defined by proteolysis studies of the intact bacterial-expressed ectodomain, was determined...
Weissenhorn, Winfried, Calder, Lesley J., Dessen, Andréa, Laue, Tom, Skehel, John J., Wiley, Don C.
The HIV-1 envelope subunit gp41 plays a role in viral entry by initiating fusion of the viral and cellular membranes. A chimeric molecule was constructed centered on the ectodomain of gp41 without...
Gauthier, Laurent, Smith, Kathrine J., Pyrdol, Jason, Kalandadze, Avtandil, Strominger, Jack L., Wiley, Don C., ...
HLA-DR2 is associated with susceptibility to multiple sclerosis (MS). A peptide from human myelin basic protein (MBP, residues 85–99) was previously found to bind to purified HLA-DR2 (DRA,...
Structure of a soluble secreted chemokine inhibitor vCCI (p35) from cowpox virus
Carfí, Andrea, Smith, Craig A., Smolak, Pamela J., McGrew, Jeffrey, Wiley, Don C.
Most poxviruses, including variola, the causative agent of smallpox, express a secreted protein of 35 kDa, vCCI, which binds CC-chemokines with high affinity. This viral protein competes with the...
Weissenhorn, Winfried, Calder, Lesley J., Wharton, Stephen A., Skehel, John J., Wiley, Don C.
The ectodomain of the Ebola virus Gp2 glycoprotein was solubilized with a trimeric, isoleucine zipper derived from GCN4 (pIIGCN4) in place of the hydrophobic fusion peptide at the N terminus. This...
Zinc-dependent dimers observed in crystals of human endostatin
Ding, Yuan-Hua, Javaherian, Kashi, Lo, Kin-Ming, Chopra, Rajiv, Boehm, Thomas, Lanciotti, Julia, ...
The crystal structure of human endostatin reveals a zinc-binding site. Atomic absorption spectroscopy indicates that zinc is a constituent of both human and murine endostatin in solution. The human...
Gewurz, Benjamin E., Gaudet, Rachelle, Tortorella, Domenico, Wang, Evelyn W., Ploegh, Hidde L., Wiley, Don C.
Many persistent viruses have evolved the ability to subvert MHC class I antigen presentation. Indeed, human cytomegalovirus (HCMV) encodes at least four proteins that down-regulate cell-surface...
González-Reyes, Luis, Ruiz-Argüello, M. Begoña, García-Barreno, Blanca, Calder, Leslie, López, Juan A., Albar, Juan P., ...
Preparations of purified full-length fusion (F) protein of human respiratory syncytial virus (HRSV) expressed in recombinant vaccinia-F infected cells, or of an anchorless mutant (FTM−) lacking the...
Ha, Ya, Stevens, David J., Skehel, John J., Wiley, Don C.
The three-dimensional structures of avian H5 and swine H9 influenza hemagglutinins (HAs) from viruses closely related to those that caused outbreaks of human disease in Hong Kong in 1997 and 1999...
Studies on influenza haemagglutinin fusion peptide mutants generated by reverse genetics
Cross, Karen J., Wharton, Stephen A., Skehel, John J., Wiley, Don C., Steinhauer, David A.
Influenza haemagglutinin (HA) is responsible for fusing viral and endosomal membranes during virus entry. In this process, conformational changes in the HA relocate the HA2 N-terminal ‘fusion...
Structure of the ABC ATPase domain of human TAP1, the transporter associated with antigen processing
Gaudet, Rachelle, Wiley, Don C.
The transporter associated with antigen processing (TAP) is an ABC transporter formed of two subunits, TAP1 and TAP2, each of which has an N-terminal membrane-spanning domain and a C-terminal ABC...
Ha, Ya, Stevens, David J., Skehel, John J., Wiley, Don C.
There are 15 subtypes of influenza A virus (H1–H15), all of which are found in avian species. Three caused pandemics in the last century: H1 in 1918 (and 1977), H2 in 1957 and H3 in 1968. In 1997,...
Connolly, Sarah A., Landsburg, Daniel J., Carfi, Andrea, Wiley, Don C., Eisenberg, Roselyn J., Cohen, Gary H.
Binding of herpes simplex virus (HSV) envelope glycoprotein D (gD) to a cell surface receptor is an essential step of virus entry. We recently determined the crystal structure of gD bound to one...
Connolly, Sarah A., Landsburg, Daniel J., Carfi, Andrea, Wiley, Don C., Cohen, Gary H., Eisenberg, Roselyn J.
Herpes simplex virus (HSV) entry into cells requires the binding of glycoprotein D (gD) to one of several cell surface receptors. The crystal structure of gD bound to one of these receptors,...
Hennecke, Jens, Carfi, Andrea, Wiley, Don C.
An αβ T-cell receptor (αβTCR)/hemagglutinin (HA) peptide/human leukocyte antigen (HLA)-DR1 complex was stabilized by flexibly linking the HA peptide with the human HA1.7 αβTCR, to increase the...
Chen, Bing, Cheng, Yifan, Calder, Lesley, Harrison, Stephen C., Reinherz, Ellis L., Skehel, John J., ...
The envelope glycoproteins of the human immunodeficiency virus and the related simian immunodeficiency virus (SIV) mediate viral entry into host cells by fusing viral and target cell membranes. We...
Aspartate Transcarbamoylase from Escherichia coli: Electron Density at 5.5 Å Resolution
Warren, Stephen G., Edwards, Brian F. P., Evans, David R., Wiley, Don C., Lipscomb, William N.
The allosteric enzyme, aspartate transcarbamoylase (EC 2.1.3.2), has previously been shown in our x-ray diffraction studies to have D3-32 symmetry. There are six catalytic (C) and six regulatory (R)...
Edwards, Brian F. P., Evans, David R., Warren, Stephen G., Monaco, Hugo L., Landfear, Scott M., Eisele, George, ...
Following our earlier determination of the three-dimensional structure of aspartate carbamoyltransferase (EC 2.1.3.2; carbamoylphosphate: L-aspartate carbamoyltransferase) to 5.5-Å resolution [S. G....
Crystal structure of a human CD3-ε/δ dimer in complex with a UCHT1 single-chain antibody fragment
Arnett, Kelly L., Harrison, Stephen C., Wiley, Don C.
The α/β T cell receptor complex transmits signals from MHC/peptide antigens through a set of constitutively associated signaling molecules, including CD3-ε/γ and CD3-ε/δ. We report the crystal...
Potential Nectin-1 Binding Site on Herpes Simplex Virus Glycoprotein D
Connolly, Sarah A., Landsburg, Daniel J., Carfi, Andrea, Whitbeck, J. Charles, Zuo, Yi, Wiley, Don C., ...
Four glycoproteins (gD, gB, gH, and gL) are essential for herpes simplex virus (HSV) entry into cells. An early step of fusion requires gD to bind one of several receptors, such as nectin-1 or...
Structure of unliganded HSV gD reveals a mechanism for receptor-mediated activation of virus entry
Krummenacher, Claude, Supekar, Vinit M, Whitbeck, J Charles, Lazear, Eric, Connolly, Sarah A, Eisenberg, Roselyn J, ...
Herpes simplex virus (HSV) entry into cells requires binding of the envelope glycoprotein D (gD) to one of several cell surface receptors. The 50 C-terminal residues of the gD ectodomain are...
Chen, Jue, Skehel, John J., Wiley, Don C.
The structure of a stable recombinant ectodomain of influenza hemagglutinin HA2 subunit, EHA2 (23–185), defined by proteolysis studies of the intact bacterial-expressed ectodomain, was determined...
Weissenhorn, Winfried, Calder, Lesley J., Dessen, Andréa, Laue, Tom, Skehel, John J., Wiley, Don C.
The HIV-1 envelope subunit gp41 plays a role in viral entry by initiating fusion of the viral and cellular membranes. A chimeric molecule was constructed centered on the ectodomain of gp41 without...
Gauthier, Laurent, Smith, Kathrine J., Pyrdol, Jason, Kalandadze, Avtandil, Strominger, Jack L., Wiley, Don C., ...
HLA-DR2 is associated with susceptibility to multiple sclerosis (MS). A peptide from human myelin basic protein (MBP, residues 85–99) was previously found to bind to purified HLA-DR2 (DRA,...
Structure of a soluble secreted chemokine inhibitor vCCI (p35) from cowpox virus
Carfí, Andrea, Smith, Craig A., Smolak, Pamela J., McGrew, Jeffrey, Wiley, Don C.
Most poxviruses, including variola, the causative agent of smallpox, express a secreted protein of 35 kDa, vCCI, which binds CC-chemokines with high affinity. This viral protein competes with the...
Weissenhorn, Winfried, Calder, Lesley J., Wharton, Stephen A., Skehel, John J., Wiley, Don C.
The ectodomain of the Ebola virus Gp2 glycoprotein was solubilized with a trimeric, isoleucine zipper derived from GCN4 (pIIGCN4) in place of the hydrophobic fusion peptide at the N terminus. This...
Zinc-dependent dimers observed in crystals of human endostatin
Ding, Yuan-Hua, Javaherian, Kashi, Lo, Kin-Ming, Chopra, Rajiv, Boehm, Thomas, Lanciotti, Julia, ...
The crystal structure of human endostatin reveals a zinc-binding site. Atomic absorption spectroscopy indicates that zinc is a constituent of both human and murine endostatin in solution. The human...
Gewurz, Benjamin E., Gaudet, Rachelle, Tortorella, Domenico, Wang, Evelyn W., Ploegh, Hidde L., Wiley, Don C.
Many persistent viruses have evolved the ability to subvert MHC class I antigen presentation. Indeed, human cytomegalovirus (HCMV) encodes at least four proteins that down-regulate cell-surface...
González-Reyes, Luis, Ruiz-Argüello, M. Begoña, García-Barreno, Blanca, Calder, Leslie, López, Juan A., Albar, Juan P., ...
Preparations of purified full-length fusion (F) protein of human respiratory syncytial virus (HRSV) expressed in recombinant vaccinia-F infected cells, or of an anchorless mutant (FTM−) lacking the...
Ha, Ya, Stevens, David J., Skehel, John J., Wiley, Don C.
The three-dimensional structures of avian H5 and swine H9 influenza hemagglutinins (HAs) from viruses closely related to those that caused outbreaks of human disease in Hong Kong in 1997 and 1999...
Studies on influenza haemagglutinin fusion peptide mutants generated by reverse genetics
Cross, Karen J., Wharton, Stephen A., Skehel, John J., Wiley, Don C., Steinhauer, David A.
Influenza haemagglutinin (HA) is responsible for fusing viral and endosomal membranes during virus entry. In this process, conformational changes in the HA relocate the HA2 N-terminal ‘fusion...
Structure of the ABC ATPase domain of human TAP1, the transporter associated with antigen processing
Gaudet, Rachelle, Wiley, Don C.
The transporter associated with antigen processing (TAP) is an ABC transporter formed of two subunits, TAP1 and TAP2, each of which has an N-terminal membrane-spanning domain and a C-terminal ABC...
Ha, Ya, Stevens, David J., Skehel, John J., Wiley, Don C.
There are 15 subtypes of influenza A virus (H1–H15), all of which are found in avian species. Three caused pandemics in the last century: H1 in 1918 (and 1977), H2 in 1957 and H3 in 1968. In 1997,...
Connolly, Sarah A., Landsburg, Daniel J., Carfi, Andrea, Wiley, Don C., Eisenberg, Roselyn J., Cohen, Gary H.
Binding of herpes simplex virus (HSV) envelope glycoprotein D (gD) to a cell surface receptor is an essential step of virus entry. We recently determined the crystal structure of gD bound to one...
Connolly, Sarah A., Landsburg, Daniel J., Carfi, Andrea, Wiley, Don C., Cohen, Gary H., Eisenberg, Roselyn J.
Herpes simplex virus (HSV) entry into cells requires the binding of glycoprotein D (gD) to one of several cell surface receptors. The crystal structure of gD bound to one of these receptors,...
Hennecke, Jens, Carfi, Andrea, Wiley, Don C.
An αβ T-cell receptor (αβTCR)/hemagglutinin (HA) peptide/human leukocyte antigen (HLA)-DR1 complex was stabilized by flexibly linking the HA peptide with the human HA1.7 αβTCR, to increase the...
Chen, Bing, Cheng, Yifan, Calder, Lesley, Harrison, Stephen C., Reinherz, Ellis L., Skehel, John J., ...
The envelope glycoproteins of the human immunodeficiency virus and the related simian immunodeficiency virus (SIV) mediate viral entry into host cells by fusing viral and target cell membranes. We...
Aspartate Transcarbamoylase from Escherichia coli: Electron Density at 5.5 Å Resolution
Warren, Stephen G., Edwards, Brian F. P., Evans, David R., Wiley, Don C., Lipscomb, William N.
The allosteric enzyme, aspartate transcarbamoylase (EC 2.1.3.2), has previously been shown in our x-ray diffraction studies to have D3-32 symmetry. There are six catalytic (C) and six regulatory (R)...
Edwards, Brian F. P., Evans, David R., Warren, Stephen G., Monaco, Hugo L., Landfear, Scott M., Eisele, George, ...
Following our earlier determination of the three-dimensional structure of aspartate carbamoyltransferase (EC 2.1.3.2; carbamoylphosphate: L-aspartate carbamoyltransferase) to 5.5-Å resolution [S. G....
Crystal structure of a human CD3-ε/δ dimer in complex with a UCHT1 single-chain antibody fragment
Arnett, Kelly L., Harrison, Stephen C., Wiley, Don C.
The α/β T cell receptor complex transmits signals from MHC/peptide antigens through a set of constitutively associated signaling molecules, including CD3-ε/γ and CD3-ε/δ. We report the crystal...
Potential Nectin-1 Binding Site on Herpes Simplex Virus Glycoprotein D
Connolly, Sarah A., Landsburg, Daniel J., Carfi, Andrea, Whitbeck, J. Charles, Zuo, Yi, Wiley, Don C., ...
Four glycoproteins (gD, gB, gH, and gL) are essential for herpes simplex virus (HSV) entry into cells. An early step of fusion requires gD to bind one of several receptors, such as nectin-1 or...
Structure of unliganded HSV gD reveals a mechanism for receptor-mediated activation of virus entry
Krummenacher, Claude, Supekar, Vinit M, Whitbeck, J Charles, Lazear, Eric, Connolly, Sarah A, Eisenberg, Roselyn J, ...
Herpes simplex virus (HSV) entry into cells requires binding of the envelope glycoprotein D (gD) to one of several cell surface receptors. The 50 C-terminal residues of the gD ectodomain are...
Stratikos, Efstratios, Mosyak, Lidia, Zaller, Dennis M., Wiley, Don C.
Human histocompatibility leukocyte antigen (HLA)-DM is a major histocompatibility complex (MHC)-like protein that catalyzes exchange of antigenic peptides from MHC class II molecules. To investigate...
Baker, Brian M., Turner, Richard V., Gagnon, Susan J., Wiley, Don C., Biddison, William E.
Structural studies have shown that class I major histocompatibility complex (MHC)-restricted peptide-specific T cell receptor (TCR)-α/βs make multiple contacts with the α1 and α2 helices of the...
The α/β T cell receptor (TCR) HA1.7 specific for the hemagglutinin (HA) antigen peptide from influenza A virus is HLA-DR1 restricted but cross-reactive for the HA peptide presented by the...
Smith, Kathrine J., Pyrdol, Jason, Gauthier, Laurent, Wiley, Don C., Wucherpfennig, Kai W.
Susceptibility to multiple sclerosis is associated with the human histocompatibility leukocyte antigen (HLA)-DR2 (DRB1*1501) haplotype. The structure of HLA-DR2 was determined with a bound peptide...
The crystal structure of the human class I major histocompatibility complex molecule, human histocompatibility leukocyte antigen (HLA)-Cw4, the ligand for a natural killer (NK) cell inhibitory...