Shi, Xianzong, Harrison, Robert L, Hollister, Jason R, Mohammed, Ahmed, Fraser, Malcolm J, Jarvis, Donald L
Abstract Background We constructed and characterized several new piggyBac vectors to provide transposition of constitutively- or inducibly-expressible heterologous gene pairs. The dual constitutive...
Aumiller, Jared J., Hollister, Jason R., Jarvis, Donald L.
We previously engineered transgenic insect cell lines to express mammalian glycosyltransferases and showed that these cells can sialylate N-glycoproteins, despite the fact that they have little...
Evidence for a sialic acid salvaging pathway in lepidopteran insect cells (2003)
Hollister, Jason, Conradt, Harald, Jarvis, Donald L.
We previously described a transgenic insect cell line, Sfβ4GalT/ST6 that expresses mammalian β-1,4-galactosyltransferase and α2,6-sialyltransferase genes and produces glycoproteins with terminally...
Evidence for a sialic acid salvaging pathway in lepidopteran insect cells (2003)
Hollister, Jason, Conradt, Harald, Jarvis, Donald L.
We previously described a transgenic insect cell line, Sfβ4GalT/ST6, that expresses mammalian β-1,4-galactosyltransferase and α2,6-sialyltransferase genes and produces glycoproteins with...
Aumiller, Jared J., Hollister, Jason R., Jarvis, Donald L.
We have previously engineered transgenic insect cell lines to express mammalian glycosyltransferases and showed that these cells can sialylate N-glycoproteins, despite the fact that they have little...
Tomiya, Noboru, Howe, Dale, Aumiller, Jared J., Pathak, Manuj, Park, Jung, Palter, Karen B., ...
A novel recombinant baculovirus expression vector was used to produce His-tagged human transferrin in a transformed insect cell line (Tn5β4GalT) that constitutively expresses a mammalian...
Aumiller, Jared J., Hollister, Jason R., Jarvis, Donald L.
We previously engineered transgenic insect cell lines to express mammalian glycosyltransferases and showed that these cells can sialylate N-glycoproteins, despite the fact that they have little...
Evidence for a sialic acid salvaging pathway in lepidopteran insect cells (2003)
Hollister, Jason, Conradt, Harald, Jarvis, Donald L.
We previously described a transgenic insect cell line, Sfβ4GalT/ST6 that expresses mammalian β-1,4-galactosyltransferase and α2,6-sialyltransferase genes and produces glycoproteins with terminally...
Biosynthesis and processing of Spodoptera frugiperda{alpha}-mannosidase III (2002)
Francis, Brian R., Paquin, Laura, Weinkauf, Carla, Jarvis, Donald L.
We previously cloned a lepidopteran insect cell cDNA that encodes a class II α-mannosidase that is localized in the Golgi apparatus but is cobalt-dependent, has a neutral pH optimum, hydrolyzes...
Vadaie, Nadia, Hulinsky, Rebecca S., Jarvis, Donald L.
Drosophila melanogaster is widely considered to be an attractive model organism for studying the functions of the carbohydrate moieties of glycoconjugates produced by higher eukaryotes. However, the...
Tomiya, Noboru, Howe, Dale, Aumiller, Jared J., Pathak, Manuj, Park, Jung, Palter, Karen, ...
A novel recombinant baculovirus expression vector was used to produce His-tagged human transferrin in a transformed insect cell line (Tn5β4GalT) that constitutively expresses a mammalian...
R. Hollister, Jason, Jarvis, Donald L.
Recombinant mammalian glycoproteins produced by the baculovirus–insect cell expression system usually do not have structurally authentic glycans. One reason for this limitation is the virtual...
Ailor, Eric, Takahashi, Noriko, Tsukamoto, Yoshinori, Masuda, Katsuyoshi, Rahman, Badarulhisam Abdul, Jarvis, Donald L., ...
The N-glycans of human serum transferrin produced in Trichopulsia ni cells were analyzed to examine N-linked oligosaccharide processing in insect cells. Metabolic radiolabeling of the intra- and...
N-Glycan processing by a lepidopteran insect {alpha}1,2-mannosidase (2000)
Kawar, Ziad, Romero, Pedro A., Herscovics, Annette, Jarvis, Donald L.
Protein glycosylation pathways are relatively poorly characterized in insect cells. As part of an overall effort to address this problem, we previously isolated a cDNA from Sf9 cells that encodes an...
Jarvis, Donald L., Bohlmeyer, Dwight A., Liao, Yung-Feng, Lomax, Kristen K., Merkle, Roberta K., Weinkauf, Carla, ...
Lepidopteran insect cells are used routinely as hosts for foreign glycoprotein expression by recombinant baculoviruses, but the precise nature of their N-glycosylation pathway remains poorly defined....
Kawar, Ziad, Herscovics, Annette, Jarvis, Donald L.
As part of our ongoing efforts to characterize the N-glycosylation pathway of lepidopteran insect cells, we have isolated an α1,2-mannosidase homolog from an Sf9 cDNA library. This cDNA contains an...
Bibliography: leaves 180-215.
Mutational Analysis of the N-Linked Glycans on Autographa californica Nucleopolyhedrovirus gp64
Jarvis, Donald L., Wills, Liz, Burow, Gloria, Bohlmeyer, Dwight A.
gp64 is the major envelope glycoprotein in the budded form of Autographa californica multicapsid nucleopolyhedrovirus (AcMNPV). gp64 is essential for AcMNPV infection, as it mediates penetration of...
Jarvis, Donald L., Howe, Dale, Aumiller, Jared J.
This report describes novel baculovirus vectors designed to express mammalian β1,4-galactosyltransferase and α2,6-sialyltransferase genes at early times after infection. Sf9 cells infected with...
Washburn, Jan O., Chan, Eric Y., Volkman, Loy E., Aumiller, Jared J., Jarvis, Donald L.
Autographa californica multicapsid nucleopolyhedrovirus (AcMNPV), the type species of the Nucleopolyhedrovirus genus (Baculoviridae family), has two highly unusual traits shared by several...
Mutational Analysis of the N-Linked Glycans on Autographa californica Nucleopolyhedrovirus gp64
Jarvis, Donald L., Wills, Liz, Burow, Gloria, Bohlmeyer, Dwight A.
gp64 is the major envelope glycoprotein in the budded form of Autographa californica multicapsid nucleopolyhedrovirus (AcMNPV). gp64 is essential for AcMNPV infection, as it mediates penetration of...
Jarvis, Donald L., Howe, Dale, Aumiller, Jared J.
This report describes novel baculovirus vectors designed to express mammalian β1,4-galactosyltransferase and α2,6-sialyltransferase genes at early times after infection. Sf9 cells infected with...
Washburn, Jan O., Chan, Eric Y., Volkman, Loy E., Aumiller, Jared J., Jarvis, Donald L.
Autographa californica multicapsid nucleopolyhedrovirus (AcMNPV), the type species of the Nucleopolyhedrovirus genus (Baculoviridae family), has two highly unusual traits shared by several...
Shi, Xianzong, Harrison, Robert L, Hollister, Jason R, Mohammed, Ahmed, Fraser, Malcolm J, Jarvis, Donald L
A fused lobes Gene Encodes the Processing β-N-Acetylglucosaminidase in Sf9 Cells*S⃞
Geisler, Christoph, Aumiller, Jared J., Jarvis, Donald L.
Manα6(Manα3)Manβ4GlcNAcβ4GlcNAc-R is the core structure of the major processed protein N-glycans produced by insect cells. Ultimately, this paucimannose type structure is produced by an unusual...