Masako Ogura, J. Christian Perez, Hae-Kyung Lee, Geidrius Dailide, Shumin Tan, ...
Geographic partitioning is postulated to foster divergence of Helicobacter pylori populations as an adaptive response to local differences in predominant host physiology. H. pylori's ability to...
Functional Adaptation of BabA, the H. pylori ABO Blood Group Antigen Binding Adhesin (2004)
Aspholm-Hurtig, Marina, Dailide, Giedrius, Lahmann, Martina, Kalia, Awdhesh, Ilver, Dag, Roche, Niamh, ...
Adherence by Helicobacter pylori increases the risk of gastric disease. Here, we report that more than 95% of strains that bind fucosylated blood group antigen bind A, B, and O antigens...
Helicobacter pylori SabA adhesin in persistent infection and chronic inflammation (2002)
Mahdavi, Jafar, Sondén, Berit, Hurtig, Marina, Olfat, Farzad O., Forsberg, Lina, Roche, Niamh, ...
Helicobacter pylori adherence in the human gastric mucosa involves specific bacterial adhesins and cognate host receptors. Here, we identify sialyl-dimeric-Lewis x glycosphingolipid as a receptor for...
Differences in genotypes of Helicobacter pylori from different human populations. (2000)
Kersulyte, Dangeruta, Mukhopadhyay, Asish K., Velapati O, Billie, Su, WanWen, Pan, ZhiJun, Garcia, Claudia, ...
DNA motifs at several informative loci in more than 500 strains of Helicobacter pylori from five continents were studied by PCR and sequencing to gain insights into the evolution of this gastric...
Mobile DNA / editors Douglas E. Berg and Martha M. Howe (1989)
Berg, Douglas E, Howe, Martha M
Incluye índice
Recombinant formation in partially diploid strains of Escherichia coli. (1969)
Thesis (Ph. D.)--University of Washington.
Thesis (M.S.)--South Dakota State University, 1960.
Epithelial attachment alters the outcome of Helicobacter pylori infection
Guruge, Janaki L., Falk, Per G., Lorenz, Robin G., Dans, Maria, Wirth, Hans-Peter, Blaser, Martin J., ...
Genetically defined in vivo models are needed to assess the importance of target cell attachment in bacterial pathogenesis. Gastric colonization by Helicobacter pylori in human populations is common...
Akopyants, Natalia S., Fradkov, Arkady, Diatchenko, Luda, Hill, Jason E., Siebert, Paul D., Lukyanov, Sergey A., ...
Genes that are characteristic of only certain strains of a bacterial species can be of great biologic interest. Here we describe a PCR-based subtractive hybridization method for efficiently detecting...
Caviedes, Luz, Lee, Tien-Shun, Gilman, Robert H., Sheen, Patricia, Spellman, Emily, Lee, Ellen H., ...
Inexpensive, rapid, and reliable methods of detecting infection by and drug susceptibility of Mycobacterium tuberculosis (MTB) are crucial to the control of tuberculosis. The novel microscopic...
Debets-Ossenkopp, Yvette J., Pot, Raymond G. J., Van Westerloo, David J., Goodwin, Avery, Berg, Douglas E., ...
We found that NCTC11637, the type strain of Helicobacter pylori, the causative agent of peptic ulcer disease and an early risk factor for gastric cancer, is metronidazole resistant. DNA...
Fused and Overlapping rpoB and rpoC Genes in Helicobacters, Campylobacters, and Related Bacteria
Zakharova, Natalya, Paster, Bruce J., Wesley, Irene, Dewhirst, Floyd E., Berg, Douglas E., Severinov, Konstantin V.
The genes coding for the β (rpoB) and β′ (rpoC) subunits of RNA polymerase are fused in the gastric pathogen Helicobacter pylori but separate in other taxonomic groups. To better understand how...
Differences in Genotypes of Helicobacter pylori from Different Human Populations
Kersulyte, Dangeruta, Mukhopadhyay, Asish K., Velapatiño, Billie, Su, WanWen, Pan, ZhiJun, Garcia, Claudia, ...
DNA motifs at several informative loci in more than 500 strains of Helicobacter pylori from five continents were studied by PCR and sequencing to gain insights into the evolution of this gastric...
Distinctiveness of Genotypes of Helicobacter pylori in Calcutta, India
Mukhopadhyay, Asish K., Kersulyte, Dangeruta, Jeong, Jin-Yong, Datta, Simanti, Ito, Yoshiyuki, Chowdhury, Abhijit, ...
The genotypes of 78 strains of Helicobacter pylori from Calcutta, India (55 from ulcer patients and 23 from more-benign infections), were studied, with a focus on putative virulence genes and neutral...
Jeong, Jin-Yong, Mukhopadhyay, Asish K., Dailidiene, Daiva, Wang, Yipeng, Velapatiño, Billie, Gilman, Robert H., ...
Helicobacter pylori is a human-pathogenic bacterial species that is subdivided geographically, with different genotypes predominating in different parts of the world. Here we test and extend an...
Sisson, Gary, Jeong, Jin-Yong, Goodwin, Avery, Bryden, Louis, Rossler, Norma, Lim-Morrison, Sabrina, ...
Much of the normal high sensitivity of wild-type Helicobacter pylori to metronidazole (Mtz) depends on rdxA (HP0954), a gene encoding a novel nitroreductase that catalyzes the conversion of Mtz from...
Specificities of Eleven Different DNA Methyltransferases of Helicobacter pylori Strain 26695
Vitkute, Jolanta, Stankevicius, Kornelijus, Tamulaitiene, Giedre, Maneliene, Zita, Timinskas, Albertas, Berg, Douglas E., ...
Methyltransferases (MTases) of procaryotes affect general cellular processes such as mismatch repair, regulation of transcription, replication, and transposition, and in some cases may be essential...
Jeong, Jin-Yong, Mukhopadhyay, Asish K., Akada, Junko K., Dailidiene, Daiva, Hoffman, Paul S., Berg, Douglas E.
The relative importance of the frxA and rdxA nitroreductase genes of Helicobacter pylori in metronidazole (MTZ) susceptibility and resistance has been controversial. Jeong et al. (J. Bacteriol....
McAtee, C. Patrick, Lim, Moon Young, Fung, Kevin, Velligan, Mark, Fry, Kirk, Chow, Theresa, ...
There is great interest in characterizing the proteins of the gastric pathogen Helicobacter pylori, especially those to which humans respond immunologically, because of the potential importance of...
Li, Shude D., Kersulyte, Dangeruta, Lindley, Ivan J. D., Neelam, Beena, Berg, Douglas E., Crabtree, Jean E.
Helicobacter pylori strains that contain the cag pathogenicity island (PAI) elicit increased synthesis of gastric C-X-C chemokines, promote neutrophilic infiltration into the gastric epithelium, and...
Cell Vacuolation, a Manifestation of the El Tor Hemolysin of Vibrio cholerae
Mitra, Rupak, Figueroa, Paula, Mukhopadhyay, Asish K., Shimada, Toshio, Takeda, Yoshifumi, Berg, Douglas E., ...
Culture supernatants of nontoxigenic nonepidemic clinical strains of Vibrio cholerae belonging to diverse serogroups were found to induce vacuolation of nonconfluent HeLa cells. The vacuoles became...
Cell Vacuolation Caused by Vibrio cholerae Hemolysin
Figueroa-Arredondo, Paula, Heuser, John E., Akopyants, Natalia S., Morisaki, J. Hiroshi, Giono-Cerezo, Silvia, Enríquez-Rincón, Fernando, ...
Non-O1 strains of Vibrio cholerae implicated in gastroenteritis and diarrhea generally lack virulence determinants such as cholera toxin that are characteristic of epidemic strains; the factors that...
Role of Helicobacter pylori cag Region Genes in Colonization and Gastritis in Two Animal Models
Eaton, Kathryn A., Kersulyte, Dange, Mefford, Megan, Danon, Stephen J., Krakowka, Steven, Berg, Douglas E.
The Helicobacter pylori chromosomal region known as the cytotoxin-gene associated pathogenicity island (cag PAI) is associated with severe disease and encodes proteins that are believed to induce...
Mukhopadhyay, Asish K., Chakraborty, Soumen, Takeda, Yoshifumi, Nair, G. Balakrish, Berg, Douglas E.
Environmental isolates of Vibrio cholerae of eight randomly amplified polymorphic DNA (RAPD) fingerprint types from Calcutta, India, that were unusual in containing toxin-coregulated pilus or cholera...
Jeong, Jin-Yong, Berg, Douglas E.
In most strains of Helicobacter pylori, mutational inactivation of the rdxA (HP0954) gene, which encodes a nitroreductase that converts metronidazole (MTZ) from a harmless prodrug to a mutagenic and...
Kersulyte, Dangeruta, Mukhopadhyay, Asish K., Shirai, Mutsinori, Nakazawa, Teruko, Berg, Douglas E.
A search by subtractive hybridization for sequences present in only certain strains of Helicobacter pylori led to the discovery of a 2-kb transposable element to be called IS607, which further PCR...
Chattopadhyay, Santanu, Datta, Simanti, Chowdhury, Abhijit, Chowdhury, Sujit, Mukhopadhyay, Asish K., Rajendran, K., ...
We compared putative molecular markers of virulence (vacA, cagA, and iceA) of Helicobacter pylori strains isolated from 52 adult duodenal ulcer patients from West Bengal, India, with those of H....
Sisson, Gary, Goodwin, Avery, Raudonikiene, Ausra, Hughes, Nicky J., Mukhopadhyay, Asish K., Berg, Douglas E., ...
Nitazoxanide (NTZ) is a redox-active nitrothiazolyl-salicylamide prodrug that kills Helicobacter pylori and also many anaerobic bacterial, protozoan, and helminthic species. Here we describe...
Dailidiene, Daiva, Bertoli, M. Teresita, Miciuleviciene, Jolanta, Mukhopadhyay, Asish K., Dailide, Giedrius, Pascasio, Mario Alberto, ...
Tetracycline is useful in combination therapies against the gastric pathogen Helicobacter pylori. We found 6 tetracycline-resistant (Tetr) strains among 159 clinical isolates (from El Salvador,...
Kersulyte, Dangeruta, Velapatiño, Billie, Dailide, Giedrius, Mukhopadhyay, Asish K., Ito, Yoshiyuki, Cahuayme, Lizbeth, ...
A new member of the IS605 transposable element family, designated ISHp608, was found by subtractive hybridization in Helicobacter pylori. Like the three other insertion sequences (ISs) known in this...
Straubinger, Reinhard K., Greiter, Andrea, McDonough, Sean P., Gerold, Alexander, Scanziani, Eugenio, Soldati, Sabina, ...
The early consequences of Helicobacter pylori infection and the role of bacterial virulence determinants in disease outcome remain to be established. The present study sought to measure the...
Mukhopadhyay, Asish K., Jeong, Jin-Yong, Dailidiene, Daiva, Hoffman, Paul S., Berg, Douglas E.
Very few examples of metabolic regulation are known in the gastric pathogen Helicobacter pylori. An unanticipated case was suggested, however, upon finding two types of metronidazole...
Rahman, Motiur, Mukhopadhyay, Asish K., Nahar, Shamsun, Datta, Simanti, Mashhud Ahmad, Milan, Sarker, Safique, ...
The complex relation between the genotype of Helicobacter pylori and its association with clinical outcome is not well understood. Studies in the West have showed that strains expressing certain...
Cluster of Type IV Secretion Genes in Helicobacter pylori's Plasticity Zone
Kersulyte, Dangeruta, Velapatiño, Billie, Mukhopadhyay, Asish K., Cahuayme, Lizbeth, Bussalleu, Alejandro, Combe, Juan, ...
Some genes present in only certain strains of the genetically diverse gastric pathogen Helicobacter pylori may affect its phenotype and/or evolutionary potential. Here we describe a new 16.3-kb...
Rahman, Motiur, Mukhopadhyay, Asish K., Nahar, Shamsun, Datta, Simanti, Ahmad, Mian Mashhud, Sarker, Safique, ...
Datta, Simanti, Chattopadhyay, Santanu, Balakrish Nair, G., Mukhopadhyay, Asish K., Hembram, Jabaranjan, Berg, Douglas E., ...
Virulence-associated genes and neutral DNA markers of Helicobacter pylori strains from the Santhal and Oroan ethnic minorities of West Bengal, India, were studied. These people have traditionally...
Presence of Active Aliphatic Amidases in Helicobacter Species Able To Colonize the Stomach
Bury-Moné, Stéphanie, Skouloubris, Stéphane, Dauga, Catherine, Thiberge, Jean-Michel, Dailidiene, Daiva, Berg, Douglas E., ...
Ammonia production is of great importance for the gastric pathogen Helicobacter pylori as a nitrogen source, as a compound protecting against gastric acidity, and as a cytotoxic molecule. In addition...
Dailidiene, Daiva, Dailide, Giedrius, Ogura, Keiji, Zhang, Maojun, Mukhopadhyay, Asish K., Eaton, Kathryn A., ...
Insights into bacterium-host interactions and genome evolution can emerge from comparisons among related species. Here we studied Helicobacter acinonychis (formerly H. acinonyx), a species closely...
Motility of Urease-Deficient Derivatives of Helicobacter pylori
Early studies of a ureB mutant derivative of Helicobacter pylori had suggested that urease is needed for motility and that urease action helps energize flagellar rotation. Here we report experiments...
Isolation of a λdv Plasmid Carrying the Bacterial gal Operon
Berg, Douglas E., Jackson, David A., Mertz, Janet E.
A λdvgal plasmid carrying genes for controlled plasmid replication from phage λ and the bacterial gal operon was isolated as a deletion mutant of phage λgalq4, which carries the gal operon between...
Chattopadhyay, Santanu, Patra, Rajashree, Ramamurthy, T., Chowdhury, Abhijit, Santra, Amal, Dhali, G. K., ...
We developed and evaluated a simple, novel multiplex PCR assay for rapid detection of Helicobacter pylori infection and for the determination of vacA and cagA genotypes directly from gastric biopsy...
Antimicrobial Susceptibility of Helicobacter pylori Strains Isolated in Bangladesh
Nahar, Shamsun, Mukhopadhyay, Asish K., Khan, Rasel, Ahmad, Mian Mashhud, Datta, Simanti, Chattopadhyay, Santanu, ...
Antimicrobial susceptibility of 120 Helicobacter pylori isolates to metronidazole, tetracycline, clarithromycin, and amoxicillin was determined, and 77.5, 15, 10, and 6.6% of the isolates,...
Evolutionary Dynamics of Insertion Sequences in Helicobacter pylori
Kalia, Awdhesh, Mukhopadhyay, Asish K., Dailide, Giedrius, Ito, Yoshiyki, Azuma, Takeshi, Wong, Benjamin C. Y., ...
Prokaryotic insertion sequence (IS) elements behave like parasites in terms of their ability to invade and proliferate in microbial gene pools and like symbionts when they coevolve with their...
Kersulyte, Dangeruta, Kalia, Awdhesh, Zhang, MaoJun, Lee, Hae-Kyung, Subramaniam, Dharmalingam, Kiuduliene, Levute, ...
Here we describe ISHp609 of Helicobacter pylori, a new member of the IS605 mobile element family that is novel and contains two genes whose functions are unknown, jhp960 and jhp961, in addition to...
Metastability of Helicobacter pylori bab adhesin genes and dynamics in Lewis b antigen binding
Bäckström, Anna, Lundberg, Carina, Kersulyte, Dangeruta, Berg, Douglas E., Borén, Thomas, Arnqvist, Anna
Heterogeneity among Helicobacter pylori strains in gastric epithelial adherence is postulated to contribute to pathogen fitness in the physiologically diverse human population. H. pylori adherence to...
McNulty, Shannon L., Mole, Beth M., Dailidiene, Daiva, Segal, Issy, Ally, Reid, Mistry, Rajesh, ...
Helicobacter pylori is a genetically diverse bacterial species that chronically infects human stomachs and sometimes causes severe gastroduodenal disease. Studies of polymorphic DNA sequences can...
Suto, Hiroyuki, Zhang, Maojun, Berg, Douglas E.
To model establishment of Helicobacter pylori infection in infants, suckling mice were inoculated with mixtures of strains that preferentially colonize different gastric regions and coexist in vivo....
Morris, Julie M., Reasonover, Alisa L., Bruce, Michael G., Bruden, Dana L., McMahon, Brian J., Sacco, Frank D., ...
A commercially available rapid fluorescent in situ hybridization (FISH) test, (seaFAST H. pylori Combi-Kit; SeaPro Theranostics International, Lelystad, The Netherlands) was used to simultaneously...
Tan, Shumin, Fraley, Cresson D., Zhang, Maojun, Dailidiene, Daiva, Kornberg, Arthur, Berg, Douglas E.
Connections among biochemical pathways should help buffer organisms against environmental stress and affect the pace and trajectory of genome evolution. To explore these ideas, we studied...
Validation of String Test for Diagnosis of Helicobacter pylori Infections
Velapatiño, Billie, Balqui, Jacqueline, Gilman, Robert H., Bussalleu, Alejandro, Quino, Willi, Finger, S. Alison, ...
The method of recovering Helicobacter pylori DNA or viable cells absorbed on a string that a person has swallowed and that is retrieved an hour later (string test) should be a useful alternative to...
A Product of the Tn5 Transposase Gene Inhibits Transposition
Lowe, John B., Berg, Douglas E.
The bacterial transposon Tn5 possesses a regulatory mechanism that allows it to move with higher efficiency when it is first introduced into a cell than after it is established. Tn5 is a composite...
Berg, Claire M., Grullón, Carmen A., Wang, Aoquan, Whalen, William A., Berg, Douglas E.
Generalized transduction is commonly used to move transposon-induced mutations among bacterial strains by selecting for inheritance of a transposonencoded resistance determinant. Although complete...
Specificity of Transposon Tn5 Insertion
Berg, Douglas E., Schmandt, Margaret A., Lowe, John B.
Genetic mapping studies had shown that the bacterial transposon Tn5 can insert into many sites in a gene, but that some sites are preferred. To begin understanding Tn5's insertion specificity at the...
Mechanism of Is1 Transposition in E. COLI: Choice between Simple Insertion and Cointegration
Biel, Susan W., Berg, Douglas E.
Insertion element IS1 and IS1-based transposon Tn9 generate cointegrates (containing vector and target DNAs joined by duplicate copies of IS1 or Tn9) and simple insertions (containing IS1 or Tn9...
Localization of Action of the Is50-Encoded Transposase Protein
Phadnis, Suhas H., Sasakawa, Chihiro, Berg, Douglas E.
The movement of the bacterial insertion sequence IS50 and of composite elements containing direct terminal repeats of IS50 involves the two ends of IS50, designated O (outside) and I (inside), which...
Local DNA Sequence Control of Deletion Formation in Escherichia coli Plasmid Pbr322
DasGupta, Ujjala, Weston-Hafer, Kathleen, Berg, Douglas E.
The specificity of deletion formation was studied using tests involving reversion of palindromic insertion mutations. Insertions of a Tn 5-related transposon at 13 sites in the ampicillin-resistance...
Finger, S. Alison, Velapatiño, Billie, Kosek, Margaret, Santivañez, Livia, Dailidiene, Daiva, Quino, Willi, ...
We compared the robustness and discriminatory power of the enterobacterial repetitive intergenic consensus (ERIC) and random amplified polymorphic DNA (RAPD) fingerprinting methods for detecting...
Adherent and Invasive Escherichia coli Is Associated with Granulomatous Colitis in Boxer Dogs
Simpson, Kenneth W., Dogan, Belgin, Rishniw, Mark, Goldstein, Richard E., Klaessig, Suzanne, McDonough, Patrick L., ...
The mucosa-associated microflora is increasingly considered to play a pivotal role in the pathogenesis of inflammatory bowel disease. This study explored the possibility that an abnormal mucosal...
Epithelial attachment alters the outcome of Helicobacter pylori infection
Guruge, Janaki L., Falk, Per G., Lorenz, Robin G., Dans, Maria, Wirth, Hans-Peter, Blaser, Martin J., ...
Genetically defined in vivo models are needed to assess the importance of target cell attachment in bacterial pathogenesis. Gastric colonization by Helicobacter pylori in human populations is common...