Jagu, Subhashini, Karanam, Balasubramanyam, Gambhira, Ratish, Chivukula, Sudha V., Chaganti, Revathi J., Lowy, Douglas R., ...
Background Vaccination with minor capsid protein L2 induces antibodies that cross-neutralize diverse papillomavirus types. However, neutralizing antibody titers against the papillomavirus type from...
Rrp1b, a New Candidate Susceptibility Gene for Breast Cancer Progression and Metastasis (2007)
Xiaolan Qian, Argyrios Ziogas, Alex G. Papageorge, Brenda J. Boersma, Renard C. Walker, ...
A novel candidate metastasis modifier, ribosomal RNA processing 1 homolog B (Rrp1b), was identified through two independent approaches. First, yeast two-hybrid, immunoprecipitation, and functional...
Rrp1b, a new candidate susceptibility gene for breast cancer progression and metastasis (2007)
Nigel PS Crawford, Xiaolan Qian, Argyrios Ziogas, Alex G Papageorge, Brenda J Boersma, Renard Walker, ...
Recent evidence suggests that metastasis is modulated by heritable germline polymorphisms. A novel candidate metastasis modifier, ribosomal RNA processing 1 homolog B (Rrp1b), was identified through...
Carrageenan Is a Potent Inhibitor of Papillomavirus Infection (2006)
Christopher B. Buck, Cynthia D. Thompson, Jeffrey N. Roberts, Martin Müller, Douglas R. Lowy, John T. Schiller
Certain sexually transmitted human papillomavirus (HPV) types are causally associated with the development of cervical cancer. Our recent development of high-titer HPV pseudoviruses has made it...
Prophylactic HPV vaccines: Underlying mechanisms (2006)
Stanley, Margaret, Lowy, Douglas R., Frazer, Ian H.
Human papillomavirus virus-like particles (HPV VLP) can be generated by the synthesis and self-assembly in vitro of the major virus capsid protein L1. HPV L1 VLPs are morphologically and...
Prophylactic HPV vaccines: Underlying mechanisms (2006)
Stanley, Margaret, Lowy, Douglas R., Frazer, Ian H.
Human papillomavirus virus-like particles (HPV VLP) can be generated by the synthesis and self-assembly in vitro of the major virus capsid protein L1. HPV L1 VLPs are morphologically and...
Prophylactic HPV vaccines: Underlying mechanisms (2006)
Stanley, Margaret, Lowy, Douglas R., Frazer, Ian
Human papillomavirus virus-like particles (HPV VLP) can be generated by the synthesis and self-assembly in vitro of the major virus capsid protein L1. HPV L1 VLPs are morphologically and...
HPV-16 L1 VLP vaccine elicits a broad-spectrum of cytokine responses in whole blood (2005)
Pinto, Ligia A., Castle, Philip E., Roden, Richard B., Harro, Clayton D., Lowy, Douglas R., Schiller, John T., ...
Here, we evaluated innate and adaptive immune system cytokine responses induced by HPV-16 L1 VLP in whole blood (WB) cultures from individuals receiving the vaccine (n = 20) or placebo (n = 4) before...
HPV-16 L1 VLP vaccine elicits a broad-spectrum of cytokine responses in whole blood (2005)
Pinto, Ligia A., Castle, Philip E., Roden, Richard B., Harro, Clayton D., Lowy, Douglas R., Schiller, John T., ...
Here, we evaluated innate and adaptive immune system cytokine responses induced by HPV-16 L1 VLP in whole blood (WB) cultures from individuals receiving the vaccine (n = 20) or placebo (n = 4) before...
Chapter 16: Prophylactic Human Papillomavirus Vaccines (2003)
Lowy, Douglas R., Frazer, Ian H.
Candidate prophylactic vaccines based on papillomavirus L1 virus-like particles (VLPs) are currently in human clinical trials. The main long-term goal of the vaccine is to reduce the incidence of...
Chapter 16: Prophylactic Human Papillomavirus Vaccines (2003)
Lowy, Douglas R., Frazer, Ian H.
Candidate prophylactic vaccines based on papillomavirus L1 virus-like particles (VLPs) are currently in human clinical trials. The main long-term goal of the vaccine is to reduce the incidence of...
Nardelli-Haefliger, Denise, Wirthner, Daniel, Schiller, John T., Lowy, Douglas R., Hildesheim, Allan, Ponci, Françoise, ...
Background: In early-phase trials, a human papillomavirus 16 (HPV16) virus–like particle (VLP) vaccine has been shown to be well tolerated, immunogenic, and protective against HPV16 in women, most...
Chapter 16: Prophylactic Human Papillomavirus Vaccines (2003)
Lowy, Douglas R., Frazer, Ian H.
Candidate prophylactic vaccines based on papillomavirus L1 virus-like particles (VLPs) are currently in human clinical trials. The main long-term goal of the vaccine is to reduce the incidence of...
Harro, Clayton D., Pang, Yuk-Ying Susana, Roden, Richard B. S., Hildesheim, Allan, Wang, Zhaohui, Reynolds, Mary Jane, ...
Background: Studies in animal models have shown that systemic immunization with a papillomavirus virus-like particle (VLP) vaccine composed of L1, a major structural viral protein, can confer...
Bosch, F. Xavier, Muñoz, Nubia, De Sanjosé, Silvia, Franco, Eduardo L. F., Lowy, Douglas R., Schiffman, Mark, ...
Papillomavirus-Like Particle Vaccines (2000)
Schiller, John T., Lowy, Douglas R.
Papillomavirus-like particle (VLP)-based subunit vaccines have undergone rapid development over the past 8 years. Three types are being investigated. The most basic type is composed of only the L1...
Han, Chenglong, Qiao, Guibin, Hubbert, Nancy L., Li, Liangshou, Sun, Changsheng, Wang, Yan, ...
Background: The existence of large geographic variations in the prevalence of esophageal cancer in some countries, such as China, indicates that environmental risk factors may be important in the...
Kirnbauer, Reinhard, Hubbert, Nancy L., Wheeler, Cosette M., Becker, Thomas M., Lowy, Douglas R., Schiller, John T.
Background Previous studies have demonstrated that genital infection with high-risk types of human papillomavirus (HPV), most often HPV16, is the most significant risk factor for the development of...
Greenstone, Heather L., Nieland, John D., De Visser, Karin E., Kirnbauer, Reinhard, Roden, Richard B. S., ...
Papillomavirus-like particles (VLPs) are a promising prophylactic vaccine candidate to prevent human papillomavirus (HPV) infections and associated epithelial neoplasia. However, they are unlikely to...
Induction of autoantibodies to mouse CCR5 with recombinant papillomavirus particles
Chackerian, Bryce, Lowy, Douglas R., Schiller, John T.
The vertebrate immune system has evolved to respond vigorously to microbial infection but to ignore self-antigens. Evidence has emerged that B cell responses to viruses are initiated by immune...
Anborgh, Pieter H., Qian, Xiaolan, Papageorge, Alex G., Vass, William C., DeClue, Jeffrey E., Lowy, Douglas R.
The full-length versions of the Ras-specific exchange factors Ras-GRF1 (GRF1) and Ras-GRF2 (GRF2), which are expressed in brain and a restricted number of other organs, possess an ionomycin-dependent...
N Terminus of Sos1 Ras Exchange Factor: Critical Roles for the Dbl and Pleckstrin Homology Domains
Qian, Xiaolan, Vass, William C., Papageorge, Alex G., Anborgh, Pieter H., Lowy, Douglas R.
We have studied the functional importance of the N terminus of mouse Sos1 (mSos1), a ubiquitously expressed Ras-specific guanine nucleotide exchange factor whose C-terminal sequences bind Grb-2....
Day, Patricia M., Roden, Richard B. S., Lowy, Douglas R., Schiller, John T.
We have used immunofluorescent staining and confocal microscopy to examine the subcellular localization of structural and nonstructural bovine papillomavirus (BPV) proteins in cultured cells that...
L1 Interaction Domains of Papillomavirus L2 Necessary for Viral Genome Encapsidation
Okun, Martin M., Day, Patricia M., Greenstone, Heather L., Booy, Frank P., Lowy, Douglas R., Schiller, John T., ...
BPHE-1 cells, which harbor 50 to 200 viral episomes, encapsidate viral genome and generate infectious bovine papillomavirus type 1 (BPV1) upon coexpression of capsid proteins L1 and L2 of BPV1, but...
Positively Charged Termini of the L2 Minor Capsid Protein Are Necessary for Papillomavirus Infection
Roden, Richard B. S., Day, Patricia M., Bronzo, Brian K., Yutzy, William H., Yang, Yanqin, Lowy, Douglas R., ...
Coexpression of bovine papillomavirus L1 with L2 mutants lacking either eight N-terminal or nine C-terminal amino acids that encode positively charged domains resulted in wild-type levels of viral...
Chackerian, Bryce, Lowy, Douglas R., Schiller, John T.
High avidity and long-lasting autoantibodies to a self-polypeptide (TNF-α) were generated after parenteral vaccination of mice with low doses of virus-like particle–based (VLP-based) vaccines that...
Bliskovsky, Valery, Ramsay, Edward S., Scott, John, DuBois, Wendy, Shi, Wei, Zhang, Shuling, ...
Susceptibility to mouse plasmacytomagenesis is a complex genetic trait controlled by several Pctr loci (Pctr1, Pctr2, etc). Congenic strain analysis narrowed the genetic interval surrounding the...
Qian, Xiaolan, Esteban, Luis, Vass, William C., Upadhyaya, Cheerag, Papageorge, Alex G., Yienger, Kate, ...
Targeted disruption of both alleles of mouse sos1, which encodes a Ras-specific exchange factor, conferred mid-gestational embryonic lethality that was secondary to impaired placental development and...
Efficient Intracellular Assembly of Papillomaviral Vectors
Buck, Christopher B., Pastrana, Diana V., Lowy, Douglas R., Schiller, John T.
Although the papillomavirus structural proteins, L1 and L2, can spontaneously coassemble to form virus-like particles, currently available methods for production of L1/L2 particles capable of...
Chackerian, Bryce, Briglio, Lindsey, Albert, Paul S., Lowy, Douglas R., Schiller, John T.
Antibodies against CCR5, the major coreceptor for human immunodeficiency virus type 1 (HIV-1), may have antiviral potential as viral fusion inhibitors. In this study, we generated a virus-like...
Chattopadhyay, Sisir K., Lowy, Douglas R., Teich, Natalie M., Levine, Arthur S., Rowe, Wallace P.
The AKR mouse has a high titer of murine leukemia virus early in life, and virus-negative cells derived from embryos of this mouse strain can be activated to yield murine leukemia virus by treatment...
Qian, Xiaolan, Karpova, Tatiana, Sheppard, Allan M, McNally, James, Lowy, Douglas R
E-cadherin is an essential adhesion protein as well as a tumor suppressor that is silenced in many cancers. Its adhesion-dependent regulation of signaling has not been elucidated. We report that...
Some Implications of the Activation of Murine Leukemia Virus by Halogenated Pyrimidines
Rowe, Wallace P., Lowy, Douglas R., Teich, Natalie, Hartley, Janet W.
Genetic information of murine leukemia viruses is known to be present in essentially all mice. Delineation of the biological effects of the expression of this genetic information is complicated by...
Inhibition of Murine Leukemia Virus Replication by Poly(vinyluracil) and Poly(vinyladenine)
Pitha, Paula M., Teich, Natalie M., Lowy, Douglas R., Pitha, Josef
Poly(1-vinyluracil) and poly(9-vinyladenine), as well as the corresponding polynucleotides poly(uridylate) and poly(adenylate), inhibit acute murine leukemia virus infection in mouse-embryo cells,...
Lowy, Douglas R., Chattopadhyay, Sisir K., Teich, Natalie M., Rowe, Wallace P., Levine, Arthur S.
Studies with a single-stranded DNA probe complementary to the RNA of mouse-tropic AKR murine leukemia virus indicate that the complete genome of the AKR-type murine leukemia virus is present in the...
Day, Patricia M., Baker, Carl C., Lowy, Douglas R., Schiller, John T.
Previous studies have suggested that most papillomaviruses enter the host cell via clathrin-dependent receptor-mediated endocytosis but have not addressed later steps in viral entry. To examine these...
Maturation of Papillomavirus Capsids
Buck, Christopher B., Thompson, Cynthia D., Pang, Yuk-Ying S., Lowy, Douglas R., Schiller, John T.
The papillomavirus capsid is a nonenveloped icosahedral shell formed by the viral major structural protein, L1. It is known that disulfide bonds between neighboring L1 molecules help to stabilize the...
Prophylactic human papillomavirus vaccines
Lowy, Douglas R., Schiller, John T.
Human papillomavirus (HPV) infection causes virtually all cases of cervical cancer, the second most common cause of death from cancer among women worldwide. This Review examines prophylactic HPV...
Carrageenan Is a Potent Inhibitor of Papillomavirus Infection
Buck, Christopher B, Thompson, Cynthia D, Roberts, Jeffrey N, Müller, Martin, Lowy, Douglas R, Schiller, John T
Certain sexually transmitted human papillomavirus (HPV) types are causally associated with the development of cervical cancer. Our recent development of high-titer HPV pseudoviruses has made it...
Human α-defensins block papillomavirus infection
Buck, Christopher B., Day, Patricia M., Thompson, Cynthia D., Lubkowski, Jacek, Lu, Wuyuan, Lowy, Douglas R., ...
Sexually transmitted human papillomaviruses (HPVs) are the primary cause of cervical cancer. Recent advances in techniques for production of papillomaviral vectors [known as pseudoviruses (PsVs)]...
Richards, Rebecca M., Lowy, Douglas R., Schiller, John T., Day, Patricia M.
Papillomaviruses (PV) comprise a large family of nonenveloped DNA viruses that include the oncogenic PV types that are the causative agents of human cervical cancer. As is true of many animal DNA...
Greenstone, Heather L., Nieland, John D., De Visser, Karin E., Kirnbauer, Reinhard, Roden, Richard B. S., ...
Papillomavirus-like particles (VLPs) are a promising prophylactic vaccine candidate to prevent human papillomavirus (HPV) infections and associated epithelial neoplasia. However, they are unlikely to...
Induction of autoantibodies to mouse CCR5 with recombinant papillomavirus particles
Chackerian, Bryce, Lowy, Douglas R., Schiller, John T.
The vertebrate immune system has evolved to respond vigorously to microbial infection but to ignore self-antigens. Evidence has emerged that B cell responses to viruses are initiated by immune...
Anborgh, Pieter H., Qian, Xiaolan, Papageorge, Alex G., Vass, William C., DeClue, Jeffrey E., Lowy, Douglas R.
The full-length versions of the Ras-specific exchange factors Ras-GRF1 (GRF1) and Ras-GRF2 (GRF2), which are expressed in brain and a restricted number of other organs, possess an ionomycin-dependent...
N Terminus of Sos1 Ras Exchange Factor: Critical Roles for the Dbl and Pleckstrin Homology Domains
Qian, Xiaolan, Vass, William C., Papageorge, Alex G., Anborgh, Pieter H., Lowy, Douglas R.
We have studied the functional importance of the N terminus of mouse Sos1 (mSos1), a ubiquitously expressed Ras-specific guanine nucleotide exchange factor whose C-terminal sequences bind Grb-2....
Day, Patricia M., Roden, Richard B. S., Lowy, Douglas R., Schiller, John T.
We have used immunofluorescent staining and confocal microscopy to examine the subcellular localization of structural and nonstructural bovine papillomavirus (BPV) proteins in cultured cells that...
L1 Interaction Domains of Papillomavirus L2 Necessary for Viral Genome Encapsidation
Okun, Martin M., Day, Patricia M., Greenstone, Heather L., Booy, Frank P., Lowy, Douglas R., Schiller, John T., ...
BPHE-1 cells, which harbor 50 to 200 viral episomes, encapsidate viral genome and generate infectious bovine papillomavirus type 1 (BPV1) upon coexpression of capsid proteins L1 and L2 of BPV1, but...
Positively Charged Termini of the L2 Minor Capsid Protein Are Necessary for Papillomavirus Infection
Roden, Richard B. S., Day, Patricia M., Bronzo, Brian K., Yutzy, William H., Yang, Yanqin, Lowy, Douglas R., ...
Coexpression of bovine papillomavirus L1 with L2 mutants lacking either eight N-terminal or nine C-terminal amino acids that encode positively charged domains resulted in wild-type levels of viral...
Chackerian, Bryce, Lowy, Douglas R., Schiller, John T.
High avidity and long-lasting autoantibodies to a self-polypeptide (TNF-α) were generated after parenteral vaccination of mice with low doses of virus-like particle–based (VLP-based) vaccines that...
Bliskovsky, Valery, Ramsay, Edward S., Scott, John, DuBois, Wendy, Shi, Wei, Zhang, Shuling, ...
Susceptibility to mouse plasmacytomagenesis is a complex genetic trait controlled by several Pctr loci (Pctr1, Pctr2, etc). Congenic strain analysis narrowed the genetic interval surrounding the...
Qian, Xiaolan, Esteban, Luis, Vass, William C., Upadhyaya, Cheerag, Papageorge, Alex G., Yienger, Kate, ...
Targeted disruption of both alleles of mouse sos1, which encodes a Ras-specific exchange factor, conferred mid-gestational embryonic lethality that was secondary to impaired placental development and...
Efficient Intracellular Assembly of Papillomaviral Vectors
Buck, Christopher B., Pastrana, Diana V., Lowy, Douglas R., Schiller, John T.
Although the papillomavirus structural proteins, L1 and L2, can spontaneously coassemble to form virus-like particles, currently available methods for production of L1/L2 particles capable of...
Chackerian, Bryce, Briglio, Lindsey, Albert, Paul S., Lowy, Douglas R., Schiller, John T.
Antibodies against CCR5, the major coreceptor for human immunodeficiency virus type 1 (HIV-1), may have antiviral potential as viral fusion inhibitors. In this study, we generated a virus-like...
Chattopadhyay, Sisir K., Lowy, Douglas R., Teich, Natalie M., Levine, Arthur S., Rowe, Wallace P.
The AKR mouse has a high titer of murine leukemia virus early in life, and virus-negative cells derived from embryos of this mouse strain can be activated to yield murine leukemia virus by treatment...
Qian, Xiaolan, Karpova, Tatiana, Sheppard, Allan M, McNally, James, Lowy, Douglas R
E-cadherin is an essential adhesion protein as well as a tumor suppressor that is silenced in many cancers. Its adhesion-dependent regulation of signaling has not been elucidated. We report that...
Some Implications of the Activation of Murine Leukemia Virus by Halogenated Pyrimidines
Rowe, Wallace P., Lowy, Douglas R., Teich, Natalie, Hartley, Janet W.
Genetic information of murine leukemia viruses is known to be present in essentially all mice. Delineation of the biological effects of the expression of this genetic information is complicated by...
Inhibition of Murine Leukemia Virus Replication by Poly(vinyluracil) and Poly(vinyladenine)
Pitha, Paula M., Teich, Natalie M., Lowy, Douglas R., Pitha, Josef
Poly(1-vinyluracil) and poly(9-vinyladenine), as well as the corresponding polynucleotides poly(uridylate) and poly(adenylate), inhibit acute murine leukemia virus infection in mouse-embryo cells,...
Lowy, Douglas R., Chattopadhyay, Sisir K., Teich, Natalie M., Rowe, Wallace P., Levine, Arthur S.
Studies with a single-stranded DNA probe complementary to the RNA of mouse-tropic AKR murine leukemia virus indicate that the complete genome of the AKR-type murine leukemia virus is present in the...
Day, Patricia M., Baker, Carl C., Lowy, Douglas R., Schiller, John T.
Previous studies have suggested that most papillomaviruses enter the host cell via clathrin-dependent receptor-mediated endocytosis but have not addressed later steps in viral entry. To examine these...
Maturation of Papillomavirus Capsids
Buck, Christopher B., Thompson, Cynthia D., Pang, Yuk-Ying S., Lowy, Douglas R., Schiller, John T.
The papillomavirus capsid is a nonenveloped icosahedral shell formed by the viral major structural protein, L1. It is known that disulfide bonds between neighboring L1 molecules help to stabilize the...
Human α-defensins block papillomavirus infection
Buck, Christopher B., Day, Patricia M., Thompson, Cynthia D., Lubkowski, Jacek, Lu, Wuyuan, Lowy, Douglas R., ...
Sexually transmitted human papillomaviruses (HPVs) are the primary cause of cervical cancer. Recent advances in techniques for production of papillomaviral vectors [known as pseudoviruses (PsVs)]...
Richards, Rebecca M., Lowy, Douglas R., Schiller, John T., Day, Patricia M.
Papillomaviruses (PV) comprise a large family of nonenveloped DNA viruses that include the oncogenic PV types that are the causative agents of human cervical cancer. As is true of many animal DNA...
Prophylactic human papillomavirus vaccines
Lowy, Douglas R., Schiller, John T.
Human papillomavirus (HPV) infection causes virtually all cases of cervical cancer, the second most common cause of death from cancer among women worldwide. This Review examines prophylactic HPV...
Carrageenan Is a Potent Inhibitor of Papillomavirus Infection
Buck, Christopher B, Thompson, Cynthia D, Roberts, Jeffrey N, Müller, Martin, Lowy, Douglas R, Schiller, John T
Certain sexually transmitted human papillomavirus (HPV) types are causally associated with the development of cervical cancer. Our recent development of high-titer HPV pseudoviruses has made it...
Qian, Xiaolan, Li, Guorong, Asmussen, Holly K., Asnaghi, Laura, Vass, William C., Braverman, Richard, ...
The three deleted in liver cancer genes (DLC1–3) encode Rho-GTPase-activating proteins (RhoGAPs) whose expression is frequently down-regulated or silenced in a variety of human malignancies. The...
Rrp1b, a New Candidate Susceptibility Gene for Breast Cancer Progression and Metastasis
Crawford, Nigel P. S, Qian, Xiaolan, Ziogas, Argyrios, Papageorge, Alex G, Boersma, Brenda J, Walker, Renard C, ...
A novel candidate metastasis modifier, ribosomal RNA processing 1 homolog B (Rrp1b), was identified through two independent approaches. First, yeast two-hybrid, immunoprecipitation, and functional...
Day, Patricia M., Thompson, Cynthia D., Buck, Christopher B., Pang, Yuk-Ying S., Lowy, Douglas R., Schiller, John T.
The mechanisms of human papillomavirus (HPV) neutralization by antibodies are incompletely understood. We have used HPV16 pseudovirus infection of HaCaT cells to analyze how several neutralizing...
García-Piñeres, Alfonso, Hildesheim, Allan, Dodd, Lori, Kemp, Troy J., Williams, Marcus, Harro, Clayton, ...
To determine the systemic cytokine pattern induced by vaccination with human papillomavirus (HPV) L1 virus-like particles (VLP), we analyzed 22 different cytokines in culture supernatants of L1...
Kemp, Troy J., Hildesheim, Allan, Falk, Roni T., Schiller, John T., Lowy, Douglas R., Rodriguez, Ana Cecilia, ...
Immunogenicity evaluations in human papillomavirus (HPV) vaccine trials have relied on serological samples, yet cervical antibodies are likely to be most relevant for protection against infection. In...
Day, Patricia M., Gambhira, Ratish, Roden, Richard B. S., Lowy, Douglas R., Schiller, John T.
Pseudovirions of human papillomavirus type 16 (HPV16), the principal etiologic agent in 50% of cervical cancers, were used as a model system to investigate the cell surface interactions involved in...
Arrangement of L2 within the Papillomavirus Capsid▿
Buck, Christopher B., Cheng, Naiqian, Thompson, Cynthia D., Lowy, Douglas R., Steven, Alasdair C., Schiller, John T., ...
Papillomaviruses are a family of nonenveloped DNA tumor viruses. Some sexually transmitted human papillomavirus (HPV) types, including HPV type 16 (HPV16), cause cancer of the uterine cervix....
Heparan Sulfate-Independent Cell Binding and Infection with Furin-Precleaved Papillomavirus Capsids▿
Day, Patricia M., Lowy, Douglas R., Schiller, John T.
Papillomavirus infection normally involves virion binding to cell surface heparan sulfate proteoglycans (HSPGs). However, we found that human papillomavirus type 16 pseudovirions efficiently bound...
Jagu, Subhashini, Karanam, Balasubramanyam, Gambhira, Ratish, Chivukula, Sudha V., Chaganti, Revathi J., Lowy, Douglas R., ...
Johnson, Katherine M., Kines, Rhonda C., Roberts, Jeffrey N., Lowy, Douglas R., Schiller, John T., Day, Patricia M.
The host factors required for in vivo infection have not been investigated for any papillomavirus. Using a recently developed murine cervicovaginal challenge model, we evaluated the importance of...