Duncan J. Maskell

Modelling within-Host Spatiotemporal Dynamics of Invasive Bacterial Disease (2008)

Andrew J. Grant, Olivier Restif, Trevelyan J. McKinley, Mark Sheppard, Duncan J. Maskell, Pietro Mastroeni

Mechanistic determinants of bacterial growth, death, and spread within mammalian hosts cannot be fully resolved studying a single bacterial population. They are also currently poorly understood....

Intracellular Demography and the Dynamics of Salmonella enterica Infections (2006)

Sam P. Brown, Stephen J. Cornell, Mark Sheppard, Andrew J. Grant, Duncan J. Maskell, Bryan T. Grenfell, ...

An understanding of within-host dynamics of pathogen interactions with eukaryotic cells can shape the development of effective preventive measures and drug regimes. Such investigations have been...

Comparative analysis of the genome sequences of Bordetella pertussis, Bordetella parapertussis and Bordetella bronchiseptica (2003)

Parkhill,Julian, Sebaihia,Mohammed, Preston,Andrew, Murphy,Lee D., Thomson,Nicholas, Harris,David E., ...

Bordetella pertussis, Bordetella parapertussis and Bordetella bronchiseptica are closely related Gram-negative -proteobacteria that colonize the respiratory tracts of mammals. B. pertussis is a...

Bordetella pertussis waaA Encodes a Monofunctional 2-Keto-3-Deoxy-d-manno-Octulosonic Acid Transferase That Can Complement an Escherichia coli waaA Mutation

Isobe, Tomoko, White, Kimberley A., Allen, Andrew G., Peacock, Michael, Raetz, Christian R. H., Maskell, Duncan J.

Bordetella pertussis lipopolysaccharide (LPS) contains a single 2-keto-3-deoxy-d-manno-octulosonic acid (Kdo) residue, whereas LPS from Escherichia coli contains at least two. Here we report that B....

Genetic Basis for Lipopolysaccharide O-Antigen Biosynthesis in Bordetellae

Preston, Andrew, Allen, Andrew G., Cadisch, Joanna, Thomas, Richard, Stevens, Kim, Churcher, Carol M., ...

Bordetella bronchiseptica and Bordetella parapertussis express a surface polysaccharide, attached to a lipopolysaccharide, which has been called O antigen. This structure is absent from Bordetella...

Mutation of waaN Reduces Salmonella enterica Serovar Typhimurium-Induced Enteritis and Net Secretion of Type III Secretion System 1-Dependent Proteins

Watson, Patricia R., Benmore, Annette, Khan, Shahid A., Jones, Philip W., Maskell, Duncan J., Wallis, Timothy S.

Mutation of waaN, a gene involved in lipid A biosynthesis, reduced enteropathogenic responses induced by Salmonella enterica serovar Typhimurium in bovine ligated ileal loops. However, the secretion...

Multiple Roles for Bordetella Lipopolysaccharide Molecules during Respiratory Tract Infection

Harvill, Eric T., Preston, Andrew, Cotter, Peggy A., Allen, Andrew G., Maskell, Duncan J., Miller, Jeff F.

Bordetella pertussis, Bordetella parapertussis, and Bordetella bronchiseptica are closely related subspecies that cause respiratory tract infections in humans and other mammals and express many...

Identification and Cloning of waaF (rfaF) from Bordetella pertussis and Use To Generate Mutants of Bordetella spp. with Deep Rough Lipopolysaccharide

Allen, Andrew G., Isobe, Tomoko, Maskell, Duncan J.

A DNA locus from Bordetella pertussis capable of reconstituting lipopolysaccharide (LPS) O-antigen biosynthesis in Salmonella typhimurium SL3789 (rfaF511) has been isolated, by using selection with...

Role of Bordetella O Antigen in Respiratory Tract Infection

Burns, Valorie C., Pishko, Elizabeth J., Preston, Andrew, Maskell, Duncan J., Harvill, Eric T.

Lipopolysaccharide (LPS), as the major surface molecule of gram-negative bacteria, interacts with the host in complex ways, both inducing and protecting against aspects of inflammatory and adaptive...

Unexpected Similarities between Bordetella avium and Other Pathogenic Bordetellae

Spears, Patricia A., Temple, Louise M., Miyamoto, David M., Maskell, Duncan J., Orndorff, Paul E.

Bordetella avium causes an upper respiratory tract disease (bordetellosis) in avian species. Commercially raised turkeys are particularly susceptible. Like other pathogenic members of the genus...

Induction of Proinflammatory Responses in the Human Monocytic Cell Line THP-1 by Campylobacter jejuni

Jones, Michael A., Tötemeyer, Sabine, Maskell, Duncan J., Bryant, Clare E., Barrow, Paul A.

Campylobacter jejuni can cause an enteritis that is associated with an acute inflammatory response at the gut epithelial surface. The signals inducing inflammation are unknown. C. jejuni can...

Toll-Like Receptor Expression in C3H/HeN and C3H/HeJ Mice during Salmonella enterica Serovar Typhimurium Infection

Tötemeyer, Sabine, Foster, Neil, Kaiser, Pete, Maskell, Duncan J., Bryant, Clare E.

Here, we have investigated the mRNA expression of Toll-like receptor 2 (TLR-2), TLR-4, and MD-2 in spleens and livers of C3H/HeN mice (carrying wild-type TLR-4) and C3H/HeJ mice (carrying mutated...

Genomic and Genetic Analysis of Bordetella Bacteriophages Encoding Reverse Transcriptase-Mediated Tropism-Switching Cassettes

Liu, Minghsun, Gingery, Mari, Doulatov, Sergei R., Liu, Yichin, Hodes, Asher, Baker, Stephen, ...

Liu et al. recently described a group of related temperate bacteriophages that infect Bordetella subspecies and undergo a unique template-dependent, reverse transcriptase-mediated tropism switching...

pagP Is Required for Resistance to Antibody-Mediated Complement Lysis during Bordetella bronchiseptica Respiratory Infection

Pilione, Mylisa R., Pishko, Elizabeth J., Preston, Andrew, Maskell, Duncan J., Harvill, Eric T.

To efficiently colonize and persist in the lower respiratory tract, bacteria must survive multiple host immune mechanisms. Bordetella bronchiseptica is a gram-negative respiratory pathogen that...

Adaptation of Campylobacter jejuni NCTC11168 to High-Level Colonization of the Avian Gastrointestinal Tract

Jones, Michael A., Marston, Kerrie L., Woodall, Claire A., Maskell, Duncan J., Linton, Dennis, Karlyshev, Andrey V., ...

The genome sequence of the human pathogen Campylobacter jejuni NCTC11168 has been determined recently, but studies on colonization and persistence in chickens have been limited due to reports that...

Distribution, Genetic Diversity, and Variable Expression of the Gene Encoding Hyaluronate Lyase within the Streptococcus suis Population

King, Samantha J., Allen, Andrew G., Maskell, Duncan J., Dowson, Christopher G., Whatmore, Adrian M.

Although Streptococcus suis is an economically important pathogen of pigs and an occasional cause of zoonotic infections of humans knowledge of crucial virulence factors, and as a consequence targets...

Sublethal Infection of C57BL/6 Mice with Salmonella enterica Serovar Typhimurium Leads to an Increase in Levels of Toll-Like Receptor 1 (TLR1), TLR2, and TLR9 mRNA as Well as a Decrease in Levels of TLR6 mRNA in Infected Organs

Tötemeyer, Sabine, Kaiser, Pete, Maskell, Duncan J., Bryant, Clare E.

Sublethal infection of C57BL/6 mice with Salmonella enterica serovar Typhimurium M525P initiates a strong inflammatory response. We measured organ expression of mRNA for Toll-like receptors and their...

Identification and Functional Characterization of Chicken Toll-Like Receptor 5 Reveals a Fundamental Role in the Biology of Infection with Salmonella enterica Serovar Typhimurium†

Iqbal, Muhammad, Philbin, Victoria J., Withanage, G. S. K., Wigley, Paul, Beal, Richard K., Goodchild, Marianne J., ...

Toll-like receptors (TLRs) are a major component of the pattern recognition receptor repertoire that detect invading microorganisms and direct the vertebrate immune system to eliminate infection. In...

Signature-Tagged Transposon Mutagenesis Studies Demonstrate the Dynamic Nature of Cecal Colonization of 2-Week-Old Chickens by Campylobacter jejuni†

Grant, Andrew J., Coward, Christopher, Jones, Michael A., Woodall, Claire A., Barrow, Paul A., Maskell, Duncan J.

We have constructed plasmids to be used for in vitro signature-tagged mutagenesis (STM) of Campylobacter jejuni and used these to generate STM libraries in three different strains. Statistical...

Phase-Variable Surface Structures Are Required for Infection of Campylobacter jejuni by Bacteriophages

Coward, Chris, Grant, Andrew J., Swift, Craig, Philp, Jennifer, Towler, Rebecca, Heydarian, Mohammad, ...

This study characterizes the interaction between Campylobacter jejuni and the 16 phages used in the United Kingdom typing scheme by screening spontaneous mutants of the phage-type strains and...

Intracellular Demography and the Dynamics of Salmonella enterica Infections

Brown, Sam P, Cornell, Stephen J, Sheppard, Mark, Grant, Andrew J, Maskell, Duncan J, Grenfell, Bryan T, ...

An understanding of within-host dynamics of pathogen interactions with eukaryotic cells can shape the development of effective preventive measures and drug regimes. Such investigations have been...

Bordetella pertussis waaA Encodes a Monofunctional 2-Keto-3-Deoxy-d-manno-Octulosonic Acid Transferase That Can Complement an Escherichia coli waaA Mutation

Isobe, Tomoko, White, Kimberley A., Allen, Andrew G., Peacock, Michael, Raetz, Christian R. H., Maskell, Duncan J.

Bordetella pertussis lipopolysaccharide (LPS) contains a single 2-keto-3-deoxy-d-manno-octulosonic acid (Kdo) residue, whereas LPS from Escherichia coli contains at least two. Here we report that B....

Genetic Basis for Lipopolysaccharide O-Antigen Biosynthesis in Bordetellae

Preston, Andrew, Allen, Andrew G., Cadisch, Joanna, Thomas, Richard, Stevens, Kim, Churcher, Carol M., ...

Bordetella bronchiseptica and Bordetella parapertussis express a surface polysaccharide, attached to a lipopolysaccharide, which has been called O antigen. This structure is absent from Bordetella...

Mutation of waaN Reduces Salmonella enterica Serovar Typhimurium-Induced Enteritis and Net Secretion of Type III Secretion System 1-Dependent Proteins

Watson, Patricia R., Benmore, Annette, Khan, Shahid A., Jones, Philip W., Maskell, Duncan J., Wallis, Timothy S.

Mutation of waaN, a gene involved in lipid A biosynthesis, reduced enteropathogenic responses induced by Salmonella enterica serovar Typhimurium in bovine ligated ileal loops. However, the secretion...

Multiple Roles for Bordetella Lipopolysaccharide Molecules during Respiratory Tract Infection

Harvill, Eric T., Preston, Andrew, Cotter, Peggy A., Allen, Andrew G., Maskell, Duncan J., Miller, Jeff F.

Bordetella pertussis, Bordetella parapertussis, and Bordetella bronchiseptica are closely related subspecies that cause respiratory tract infections in humans and other mammals and express many...

Identification and Cloning of waaF (rfaF) from Bordetella pertussis and Use To Generate Mutants of Bordetella spp. with Deep Rough Lipopolysaccharide

Allen, Andrew G., Isobe, Tomoko, Maskell, Duncan J.

A DNA locus from Bordetella pertussis capable of reconstituting lipopolysaccharide (LPS) O-antigen biosynthesis in Salmonella typhimurium SL3789 (rfaF511) has been isolated, by using selection with...

Role of Bordetella O Antigen in Respiratory Tract Infection

Burns, Valorie C., Pishko, Elizabeth J., Preston, Andrew, Maskell, Duncan J., Harvill, Eric T.

Lipopolysaccharide (LPS), as the major surface molecule of gram-negative bacteria, interacts with the host in complex ways, both inducing and protecting against aspects of inflammatory and adaptive...

Unexpected Similarities between Bordetella avium and Other Pathogenic Bordetellae

Spears, Patricia A., Temple, Louise M., Miyamoto, David M., Maskell, Duncan J., Orndorff, Paul E.

Bordetella avium causes an upper respiratory tract disease (bordetellosis) in avian species. Commercially raised turkeys are particularly susceptible. Like other pathogenic members of the genus...

Induction of Proinflammatory Responses in the Human Monocytic Cell Line THP-1 by Campylobacter jejuni

Jones, Michael A., Tötemeyer, Sabine, Maskell, Duncan J., Bryant, Clare E., Barrow, Paul A.

Campylobacter jejuni can cause an enteritis that is associated with an acute inflammatory response at the gut epithelial surface. The signals inducing inflammation are unknown. C. jejuni can...

Toll-Like Receptor Expression in C3H/HeN and C3H/HeJ Mice during Salmonella enterica Serovar Typhimurium Infection

Tötemeyer, Sabine, Foster, Neil, Kaiser, Pete, Maskell, Duncan J., Bryant, Clare E.

Here, we have investigated the mRNA expression of Toll-like receptor 2 (TLR-2), TLR-4, and MD-2 in spleens and livers of C3H/HeN mice (carrying wild-type TLR-4) and C3H/HeJ mice (carrying mutated...

Genomic and Genetic Analysis of Bordetella Bacteriophages Encoding Reverse Transcriptase-Mediated Tropism-Switching Cassettes

Liu, Minghsun, Gingery, Mari, Doulatov, Sergei R., Liu, Yichin, Hodes, Asher, Baker, Stephen, ...

Liu et al. recently described a group of related temperate bacteriophages that infect Bordetella subspecies and undergo a unique template-dependent, reverse transcriptase-mediated tropism switching...

pagP Is Required for Resistance to Antibody-Mediated Complement Lysis during Bordetella bronchiseptica Respiratory Infection

Pilione, Mylisa R., Pishko, Elizabeth J., Preston, Andrew, Maskell, Duncan J., Harvill, Eric T.

To efficiently colonize and persist in the lower respiratory tract, bacteria must survive multiple host immune mechanisms. Bordetella bronchiseptica is a gram-negative respiratory pathogen that...

Adaptation of Campylobacter jejuni NCTC11168 to High-Level Colonization of the Avian Gastrointestinal Tract

Jones, Michael A., Marston, Kerrie L., Woodall, Claire A., Maskell, Duncan J., Linton, Dennis, Karlyshev, Andrey V., ...

The genome sequence of the human pathogen Campylobacter jejuni NCTC11168 has been determined recently, but studies on colonization and persistence in chickens have been limited due to reports that...

Distribution, Genetic Diversity, and Variable Expression of the Gene Encoding Hyaluronate Lyase within the Streptococcus suis Population

King, Samantha J., Allen, Andrew G., Maskell, Duncan J., Dowson, Christopher G., Whatmore, Adrian M.

Although Streptococcus suis is an economically important pathogen of pigs and an occasional cause of zoonotic infections of humans knowledge of crucial virulence factors, and as a consequence targets...

Sublethal Infection of C57BL/6 Mice with Salmonella enterica Serovar Typhimurium Leads to an Increase in Levels of Toll-Like Receptor 1 (TLR1), TLR2, and TLR9 mRNA as Well as a Decrease in Levels of TLR6 mRNA in Infected Organs

Tötemeyer, Sabine, Kaiser, Pete, Maskell, Duncan J., Bryant, Clare E.

Sublethal infection of C57BL/6 mice with Salmonella enterica serovar Typhimurium M525P initiates a strong inflammatory response. We measured organ expression of mRNA for Toll-like receptors and their...

Identification and Functional Characterization of Chicken Toll-Like Receptor 5 Reveals a Fundamental Role in the Biology of Infection with Salmonella enterica Serovar Typhimurium†

Iqbal, Muhammad, Philbin, Victoria J., Withanage, G. S. K., Wigley, Paul, Beal, Richard K., Goodchild, Marianne J., ...

Toll-like receptors (TLRs) are a major component of the pattern recognition receptor repertoire that detect invading microorganisms and direct the vertebrate immune system to eliminate infection. In...

Signature-Tagged Transposon Mutagenesis Studies Demonstrate the Dynamic Nature of Cecal Colonization of 2-Week-Old Chickens by Campylobacter jejuni†

Grant, Andrew J., Coward, Christopher, Jones, Michael A., Woodall, Claire A., Barrow, Paul A., Maskell, Duncan J.

We have constructed plasmids to be used for in vitro signature-tagged mutagenesis (STM) of Campylobacter jejuni and used these to generate STM libraries in three different strains. Statistical...

Phase-Variable Surface Structures Are Required for Infection of Campylobacter jejuni by Bacteriophages

Coward, Chris, Grant, Andrew J., Swift, Craig, Philp, Jennifer, Towler, Rebecca, Heydarian, Mohammad, ...

This study characterizes the interaction between Campylobacter jejuni and the 16 phages used in the United Kingdom typing scheme by screening spontaneous mutants of the phage-type strains and...

Intracellular Demography and the Dynamics of Salmonella enterica Infections

Brown, Sam P, Cornell, Stephen J, Sheppard, Mark, Grant, Andrew J, Maskell, Duncan J, Grenfell, Bryan T, ...

An understanding of within-host dynamics of pathogen interactions with eukaryotic cells can shape the development of effective preventive measures and drug regimes. Such investigations have been...

Differential modulatory effects of Annexin 1 on nitric oxide synthase induction by lipopolysaccharide in macrophages

Smyth, Tomoko, Harris, Hayley J, Brown, Andrew, Tötemeyer, Sabine, Farnfield, Belinda A, Maskell, Duncan J, ...

Annexin-1 (ANXA1) is a glucocorticoid-regulated protein that modulates the effects of bacterial lipopolysaccharide (LPS) on macrophages. Exogenous administration of peptides derived from the...

Mutation of the Maturase Lipoprotein Attenuates the Virulence of Streptococcus equi to a Greater Extent than Does Loss of General Lipoprotein Lipidation▿

Hamilton, Andrea, Robinson, Carl, Sutcliffe, Iain C., Slater, Josh, Maskell, Duncan J., Davis-Poynter, Nick, ...

Streptococcus equi is the causative agent of strangles, a prevalent and highly contagious disease of horses. Despite the animal suffering and economic burden associated with strangles, little is...

Predicting Protein Function from Structure—The Roles of Short-chain Dehydrogenase/Reductase Enzymes in Bordetella O-antigen Biosynthesis

King, Jerry D., Harmer, Nicholas J., Preston, Andrew, Palmer, Colin M., Rejzek, Martin, Field, Robert A., ...

The pathogenic bacteria Bordetella parapertussis and Bordetella bronchiseptica express a lipopolysaccharide O antigen containing a polymer of 2,3-diacetamido-2,3-dideoxy-l-galacturonic acid. The...