Virtual laboratories in (bio)chemical engineering education (2010)
Domingues, Lucília, Rocha, I., Dourado, Fernando, Alves, M. M., Ferreira, E. C.
In the last decades, Information and Communications Technologies (ICT) have been promoting the creation and adoption of new learning and teaching styles. Virtual laboratories, by overcoming some...
Costa, Rafael S., Machado, Daniel, Rocha, I., Ferreira, E. C.
The construction of dynamic metabolic models at reaction network level requires the use of mechanistic enzymatic rate equations that comprise a large number of parameters. The lack of knowledge on...
Mesquita, D. P., Dias, O., Amaral, A. L., Ferreira, E. C.
Different approaches using microscopy image analysis procedures were employed for characterization of activated sludge systems. The approaches varied mainly on the type of visualization and...
OptFlux : an open-source software platform for in silico metabolic engineering (2010)
Rocha, I., Maia, Paulo, Evangelista, Pedro, Vilaça, Paulo, Soares, Simão, Pinto, José P., ...
Background: Over the last few years a number of methods have been proposed for the phenotype simulation of microorganisms under different environmental and genetic conditions. These have been used as...
BioDR : semantic indexing networks for biomedical document retrieval (2010)
Lourenço, Anália, Carreira, Rafael, Glez-Peña, Daniel, Méndez, José R., Carneiro, S., Rocha, Luis M., ...
In Biomedical research, retrieving documents that match an interesting query is a task performed quite frequently. Typically, the set of obtained results is extensive containing many non-interesting...
Digging out evidences on escherichia coli stringent response from scientific literature (2009)
Carneiro, S., Lourenço, Anália, Carreira, Rafael, Rocha, Miguel, Ferreira, E. C., Rocha, I.
Costa, Rafael S., Machado, Daniel, Rocha, I., Ferreira, E. C.
Fundação para a Ciência e a Tecnologia (FCT)
A study on the effects of using gene-reaction rules on in silico strain optimization (2009)
Vilaça, Paulo, Maia, Paulo, Evangelista, Pedro, Ferreira, E. C., Rocha, Miguel, Rocha, I.
To identify a set of genetic manipulations that will result in a microbial strain with improved production capabilities of a metabolite / product of industrial interest, is one of the greatest...
Metabolic network reconstruction of the pathogen Enterococcus faecalis (2009)
Portela, Carla, Carneiro, S., Villas-Bôas, S. G., Lourenço, Anália, Rocha, I., Ferreira, E. C.
Sarraguça, M. C., Paulo, A., Alves, M. M., Dias, A. M. A., Lopes, J. A., Ferreira, E. C.
The performance of an activated sludge reactor can be significantly enhanced through use of continuous and real-time process-state monitoring, which avoids the need to sample for off-line analysis...
Application of image analysis to the prediction of EBC barley kernel weight distribution (2009)
Amaral, A. L., Rocha, Orlando, Gonçalves, Cristina, Ferreira, António Augusto, Ferreira, E. C.
It is known that the barley kernel size is an important factor regarding the uniformity of the malting and brewery processes, barley valuation, approval andmarket value. In order to facilitate the...
Carneiro, S., Villas-Bôas, S., Rocha, I., Ferreira, E. C.
Abstracts of the European Congress on Biotechnology, 14, Barcelona, Spain, 2009
Morphology and physiology of anaerobic granular sludge exposed to an organic solvent (2009)
Costa, J. C., Moita, I., Ferreira, E. C., Alves, M. M.
The use of quantitative image analysis techniques, together with physiological information might be used to monitor and detect operational problems in advance to reactor performance failure....
@Note : a workbench for biomedical text mining (2009)
Lourenço, Anália, Carreira, Rafael, Carneiro, S., Maia, Paulo, Glez-Peña, Daniel, Fdez-Riverola, Florentino, ...
Biomedical Text Mining (BioTM) is providing valuable approaches to the automated curation of scientific literature. However, most efforts have addressed the benchmarking of new algorithms rather than...
Santos, Sílvio, Carvalho, Carla M., Sillankorva, Sanna, Nicolau, Ana, Ferreira, E. C., Azeredo, Joana
Background The Double-Layer Agar (DLA) technique is extensively used in phage research to enumerate and identify phages and to isolate mutants and new phages. Many phages form large and well-defined...
Dynamic modeling of E. coli central carbon metabolism combining different kinetic rate laws (2009)
Costa, Rafael S., Machado, Daniel, Rocha, I., Ferreira, E. C.
Detailed dynamic kinetic models at the network reaction level are traditionally constructed using mechanistic enzymatic rate equations and a large number of kinetic parameters have to be determined...
Lourenço, Anália, Costa, Hugo, Carneiro, S., Carreira, Rafael, Rocha, Miguel, Ferreira, E. C., ...
Bringing biologists and text miners closer together is a major aim towards the general usage of literature mining tools. Our contribution to this aim is an end-user tool for the extraction of...
Multi-criterion approaches for the in silico optimization of mutant microorganisms (2009)
Maia, Paulo, Ferreira, E. C., Rocha, Miguel, Rocha, I.
create mutants for the production of valuable compounds is, in Metabolic Engineering, a promising, while complex task. Approaches to tackle this problem have been proposed and included MILP-based...
OptFlux : a software for metabolic engineering (2009)
Rocha, I., Maia, Paulo, Pinto, José P., Evangelista, Pedro, Nielsen, Jens, Patil, Kiran Raosaheb, ...
Mesquita, D. P., Dias, O., Dias, A. M. A., Amaral, A. L., Ferreira, E. C.
In the last years there has been an increase on the research of the activated sludge processes, and mainly on the solid–liquid separation stage, considered of critical importance, due to the...
Carneiro, S., Amaral, A. L., Veloso, Ana C. A., Dias, T., Peres, A. M., Ferreira, E. C., ...
The development of monitoring methods for assessing the physiological state of microorganisms during recombinant fermentation processes has been encouraged by the need to evaluate the influence of...
The 10th International Chemical and Biological Engineering Conference - CHEMPOR 2008, was held in Braga, Portugal, from the 4th to the 6th of September, 2008. The conference was jointly organized by...
Air pollution control with semi-infinite programming (2009)
Vaz, A. Ismael F., Ferreira, E. C.
Environment issues are more than ever important in a modern society. Complying with stricter legal thresholds on pollution emissions raises an important economic issue. This paper presents some ideas...
Monitoring of fed-batch E. coli fermentations with software sensors (2009)
Veloso, Ana C. A., Rocha, I., Ferreira, E. C.
Accurate monitoring and control of industrial bioprocess requires the knowledge of a great number of variables, being some of them not measurable with standard devices. To overcome this difficulty,...
Mesquita, D. P., Dias, O., Amaral, A. L., Ferreira, E. C.
In recent years, a great deal of attention has been focused on the research of activated sludge processes, where the solid–liquid separation phase is frequently considered of critical importance,...
Mesquita, D. P., Amaral, A. L., Ferreira, E. C., Coelho, M. A. Z.
BACKGROUND: In activated sludge systems, sludge settling ability is considered a critical step in effluent quality and determinant of solid-liquid separation processes. However, few studies have...
Costa, J. C., Moita, I., Abreu, A. A., Ferreira, E. C., Alves, M. M.
Four organic loading disturbances were performed in lab-scale EGSB reactors fed with ethanol. In load disturbance 1 (LD1) and 2 (LD2), the organic loading rate (OLR) was increased between 5 and 18.5...
Costa, J. C., Alves, M. M., Ferreira, E. C.
Principal component analysis (PCA) was applied to datasets gathering morphological, physiological and reactor performance information, from three toxic shock loads (SL1 – 1.6 mgdetergent/L; SL2 –...
Abreu, A. A., Danko, Anthony S., Costa, J. C., Ferreira, E. C., Alves, M. M.
Biohydrogen production from arabinose was examined using four different anaerobic sludges with different pHs ranging from 4.5 to 8.0. Arabinose (30 g l−1) was used as the substrate for all...
Neri, D. F. M., Balcão, V. M., Costa, Rafael S., Rocha, I., Ferreira, E. C., Torres, D., ...
The synthesis of galacto-oligosaccharides (GOS) by the action of Aspergillus oryzae β-galactosidase free and immobilized on magnetic polysiloxane-polyvinyl alcohol (mPOS-PVA) was studied. A maximum...
Pinto, José P., Dias, O., Lourenço, Anália, Carneiro, S., Ferreira, E. C., Rocha, I., ...
Genome-scale model reconstruction represents a major tool in the field of Metabolic Engineering .This paper reports on a study about data integration issues in the process of genome- scale...
Oliveira, Catarina S., Ordaz, Alberto, Alba, Joel, Alves, M. M., Ferreira, E. C., Thalasso, Frédéric
The applicability of pulse respirometry, for the estimation of kinetic and stoichiometric parameters in pure cultures was evaluated by comparison with traditional chemostat method. Pseudomonas putida...
Nobre, C., Santos, Maria João, Dominguez, Ana, Torres, D., Rocha, Orlando, Peres, A. M., ...
Adsorption equilibrium of fructose, glucose and sucrose was evaluated on sulfonated poly(styrene-co-divinylbenzene) cation-exchange resins. Two types of resins were used: potassium (K+) gel-type and...
Safety issues concerning phage therapy for veterinary applications (2009)
Azeredo, Joana, Ferreira, E. C., Gannon, B. W., Santos, Sílvio, Krylov, V. N.
Phages have been proposed as natural antimicrobial agents to fight bacterial infections in animals. This is supported by several scientific evidences of the efficacy of phage therapy for veterinary...
Costa, Rafael S., Machado, Daniel, Rocha, I., Ferreira, E. C.
One of the great challenges in the post‐genomic era is to understand the dynamic behaviour of a living cell. For that purpose, quantitative models describing metabolic network dynamics are a...
Optimization of a fructooligosaccharides purification method using activated charcoal (2009)
Nobre, C., Dominguez, Ana, Torres, D., Rocha, Orlando, Rocha, I., Ferreira, E. C., ...
Fructooligosaccharides (FOS) have gained large commercial interest due to its beneficial properties in the human health as prebiotics. FOS are produced industrially by fermentative processes....
Mesquita, D. P., Dias, O., Amaral, A. L., Ferreira, E. C.
In activated sludge systems, the sludge settling ability is considered a critical step where filamentous bulking and biomass deflocculation are the most common problems, causing the reduction of the...
Model reduction based on dynamic sensitivity analysis : a systems biology case of study (2008)
Costa, Rafael S., Rocha, I., Ferreira, E. C.
Fundação para a Ciência e a Tecnologia (FCT)
A tool for the automatic and manual annotation of biomedical documents (2008)
Lourenço, Anália, Carneiro, S., Carreira, Rafael, Rocha, Miguel, Rocha, I., Ferreira, E. C.
The techniques developed within the field of Biomedical Text Mining (BioTM) have been mainly tested and evaluated over a set of known corpora built by a few researchers with a specific goal or to...
Mathematical modeling of recombinant Escherichia coli aerobic batch fermentations (2008)
Costa, Rafael S., Rocha, I., Ferreira, E. C.
In this work, three competing unstructured mathematical models for the biomass growth by recombinant E. coli strains with different acetate inhibition kinetics terms were evaluated for batch...
Mesquita, D. P., Dias, O., Amaral, A. L., Ferreira, E. C.
Monitoring activated sludge processes by microscopic observations and image analysis methodologies is a well established technique with the utmost importance for microbial community characterization....
Costa, J. C., Alves, M. M., Ferreira, E. C.
The wide fluctuations in flow rate and the presence of toxic compounds can damage the high efficiency of high-rate anaerobic granular sludge reactors. As earlier these disturbances are detected, more...
Virtual laboratories in (bio)chemical engineering education (2008)
Domingues, Lucília, Rocha, I., Dourado, Fernando, Alves, M. M., Ferreira, E. C.
"Qualidade” of the Universidade do Minho.
Fractionation of fructo-oligosaccharides by gel filtration (2008)
Nobre, C., Santos, Maria João, Dominguez, A., Torres, D., Rodrigues, L. R., Rocha, I., ...
Neri, D. F. M., Balcão, V. M., Costa, Rafael S., Ferreira, E. C., Torres, D., Rodrigues, Lígia M., ...
β-Galactosidase from Aspergillus oryzae is an enzyme with a wide industrial application, mostly in the hydrolysis of lactose and, more recently, in the synthesis of oligosaccharides. Several...
An integrated analysis of the metabolic and regulatory networks of escherichia coli K12 (2008)
Carneiro, S., Lourenço, Anália, Mendes, Rui, Rocha, Miguel, Ferreira, E. C., Rocha, I.
Production of a salmonella bacteriophage in a non-pathogenic E. coli strain (2008)
Santos, Sílvio, Fernandes, E., Krylov, V. N., Pleteneva, E. A., Shaburova, O. V., Nicolau, Ana, ...
Implementation of a specific rate controller in a fed-batch E. coli fermentation (2008)
Rocha, I., Veloso, Ana C. A., Carneiro, S., Costa, R. S., Ferreira, E. C.
The specific growth rate is one of the most important process variables characterizing the state of microorganisms during fermentations mainly because the biosynthesis of many products of interest is...
Monitoring an activated sludge reactor using IR spectroscopy (2008)
Dias, A. M. A., Paulo, A., Mesquita, D. P., Alves, M. M., Lopes, J. A., Ferreira, E. C.
Costa, J. C., Alves, M. M., Ferreira, E. C.
Detergents and solvents are included in the list of compounds that can be inhibitory or toxic to anaerobic digestion processes. Industrial cleaning stages/processes produce vast amounts of...
Dias, O., Mesquita, D. P., Amaral, A. L., Ferreira, E. C.
Monitoring activated sludge processes by microscopic observations and image analysis is a well established technique with the utmost importance for microbial community characterization. Occasionally,...
Evaluation of activated sludge systems by image analysis procedures (2008)
Mesquita, D. P., Dias, O., Amaral, A. L., Ferreira, E. C.
Biomass inspection under optical microscopy coupled to automated image analysis methodologies is, nowadays, increasingly used. Image analysis is presently considered a powerful tool to identify and...
Paulo, A., Dias, A. M. A., Sarraguça, M. C., Lopes, J. A., Alves, M. M., Ferreira, E. C.
The performance of activated sludge reactors can be enhanced by the ability to monitor the status of the process without the need for chemicals addition or complex calibration procedures. Nowadays...
Stalked protozoa identification by image analysis and multivariable statistical techniques (2008)
Amaral, A. L., Ginoris, Y. P., Nicolau, Ana, Coelho, M. A. Z., Ferreira, E. C.
Protozoa are considered good indicators of the treatment quality in activated sludge systems as they are sensitive to physical, chemical and operational processes. Therefore, it is possible to...
Ordaz, Alberto, Oliveira, Catarina S., Aguilar, Ricardo, Carrión, Manuel, Ferreira, E. C., Alves, M. M., ...
This article proposes a simple "in-situ" pulse respirometric method for the estimation of four important kinetic and stoichiometric parameters. The method is validated in a suspended biomass...
SBR performance for synthetic and fishery wastewater treatment (2008)
Mesquita, D. P., Ribeiro, R. R., Teixeira, D., Ferreira, E. C., Coelho, M. A. Z.
The evaluation of a SBR performance treating synthetic and fishery wastewater was performed. Without salt addition, COD and ammonia removal rates over than 50% were obtained, indicating the...
Quantification of the CBD-FITC conjugates surface coating on cellulose fibres (2008)
Pinto, R., Amaral, A.L., Ferreira, E.C., Mota, M., Vilanova, M., Ruel, K., ...
Background: Cellulose Binding Domains (CBD) were conjugated with fluorescein isothiocyanate (FITC). The surface concentration of the Binding Domains adsorbed on cellulose fibres was determined by...
Quantification of the CBD-FITC conjugates surface coating on cellulose fibres (2008)
Pinto, R., Amaral, A.L., Ferreira, E.C., Mota, M., Vilanova, M., Ruel, K., ...
Background: Cellulose Binding Domains (CBD) were conjugated with fluorescein isothiocyanate (FITC). The surface concentration of the Binding Domains adsorbed on cellulose fibres was determined by...
Activated sludge process monitoring through in situ near-infrared spectral analysis (2008)
Dias, A. M. A., Moita, I., Páscoa, R., Alves, M. M., Lopes, J. A., Ferreira, E. C.
The application of near infrared (NIR) spectroscopy for industrial process monitoring is achieving increasing importance over the last twenty years. In fact, the real time monitoring capacity of NIR...
Takagi-Sugeno fuzzy observer for a switching bioprocess : sector nonlinearity approach (2008)
Herrera, Enrique, Castillo, Bernardino, Ramírez, Jesús, Ferreira, E. C.
Mexican Consejo Nacional de Ciencia y Tecnología (CONACyT).
Natural computation meta-heuristics for the in silico optimization of microbial strains (2008)
Rocha, Miguel, Maia, Paulo, Mendes, Rui, Pinto, José P., Ferreira, E. C., Nielsen, Jens, ...
Background: One of the greatest challenges in Metabolic Engineering is to develop quantitative models and algorithms to identify a set of genetic manipulations that will result in a microbial strain...
Mendes, Rui, Rocha, I., Pinto, José P., Ferreira, E. C., Rocha, Miguel
The optimization of input variables (typically feeding trajectories over time) in fed-batch fermentations has gained special attention, given the economic impact and the complexity of the problem....
The @Note biomedical text mining workbench (2008)
Lourenço, Anália, Carreira, Rafael, Carneiro, S., Maia, Paulo, Ferreira, E. C., Rocha, Miguel, ...
Network analysis of an integrated metabolic/regulatory model of Escherichia coli (2008)
Carneiro, S., Lourenço, Anália, Mendes, Rui, Rocha, Miguel, Ferreira, E. C., Rocha, I.
Quantification of the CBD-FITC conjugates surface coating on cellulose fibres (2008)
Pinto, R., Amaral, A.L., Ferreira, E.C., Mota, M., Vilanova, M., Ruel, K., ...
Background: Cellulose Binding Domains (CBD) were conjugated with fluorescein isothiocyanate (FITC). The surface concentration of the Binding Domains adsorbed on cellulose fibres was determined by...
Quantification of the CBD-FITC conjugates surface coating on cellulose fibres (2008)
Pinto, R., Amaral, A.L., Ferreira, E.C., Mota, M., Vilanova, M., Ruel, K., ...
Background: Cellulose Binding Domains (CBD) were conjugated with fluorescein isothiocyanate (FITC). The surface concentration of the Binding Domains adsorbed on cellulose fibres was determined by...
Quantification of the CBD-FITC conjugates surface coating on cellulose fibres (2008)
Pinto, R., Amaral, A.L., Ferreira, E.C., Mota, M., Vilanova, M., Ruel, K., ...
Background: Cellulose Binding Domains (CBD) were conjugated with fluorescein isothiocyanate (FITC). The surface concentration of the Binding Domains adsorbed on cellulose fibres was determined by...
Pinto, João Ricardo, Amaral, A. L., Carvalho, Joana, Ferreira, E. C., Mota, M., Gama, F. M.
The surface concentration of CBD-FITC conjugates, adsorbed on cellulose fibers, was determined by image analysis. The program consists of two scripts, the first dedicated to the elaboration of the...
An ontology-based approach to systems biology literature retrieval and processing (2007)
Lourenço, Anália, Simões, Alberto, Almeida, J. J., Rocha, Miguel, Rocha, I., Ferreira, E. C.
This paper details the SysBio Explorer, a Systems Biology Literature Retrieval and Processing Framework, whose aim relies on the automatic inference of regulatory and metabolic networks based on...
Costa, J. C., Moita, I., Abreu, A. A., Ferreira, E. C., Alves, M. M.
Fundação para a Ciência e a Tecnologia (FCT) - SFRH/BD/13317/2003; POCI/AMB/60141/2004; POCTI/BIO/37934/2001.
Literature mining applied to systems biology information retrieval (2007)
Lourenço, Anália, Simões, Alberto, Almeida, J. J., Rocha, Miguel, Rocha, I., Ferreira, E. C.
Coelho, M. A. Z., Coutinho, J. A. P., Ferreira, E. C., Mota, M., Belo, Isabel
The effects of hyperbaric gases on the cell cycle of Saccharomyces cerevisiae were studied in batch cultures under pressures between 0.1 and 0.6 MPa and different gas compositions (air, oxygen,...
Modelo exacto difuso de un proceso fermentativo conmutado (2007)
Herrera, Enrique, Ramírez, Jesús, Castillo, Bernardino, Ferreira, E. C.
Los modelos matemáticos de los procesos fermentativos son complejos y difíciles de trabajar, debido a las no linealidades presentes en el modelo. En este trabajo se realizó un modelo difuso...
Activated sludge process monitoring through in-situ NIR spectral analysis (2007)
Dias, A. M. A., Moita, I., Páscoa, R., Alves, M. M., Lopes, J. A., Ferreira, E. C.
The application of near infrared (NIR) spectroscopy for industrial process monitoring is achieving increasing importance over the last twenty years. In fact, the real time monitoring capacity of NIR...
Costa, J. C., Abreu, A. A., Ferreira, E. C., Alves, M. M.
Two shock loads of a commercial detergent (I - 150 mg chemical oxygen demand (COD)/L, fed for 56 h; II - 300 mg COD/L fed for 222 h) were applied in a lab-scale Expanded Granular Sludge Blanket...
Stalked protozoa identification by image analysis and multivariable statistical techniques (2007)
Amaral, A. L., Ginoris, Y. P., Nicolau, Ana, Coelho, M. A. Z., Ferreira, E. C.
Protozoa are considered good indicators of the treatment quality in activated sludge systems as they are sensitive to physical, chemical and operational processes. Therefore, it is possible to...
Cost minimization of a WWTP using an augmented lagrangian pattern search based solver (2007)
Araújo, M. Manuela, Ferreira, E. C.
This paper describes a derivative-free method that aims to solve an optimization problem arising from the mathematical formulation of an activated sludge system in which the objective is to minimize...
Exact fuzzy observer for a baker’s yeast fed-batch fermentation process (2007)
Herrera, Enrique, Castillo, Bernardino, Ramírez, Jesús, Ferreira, E. C.
The purpose of this work is to design an exact fuzzy observer for a bioprocess switching between two different metabolic states. A fed-batch baker's yeast culture is modeled by two sub-models: a...
Ginoris, Y. P., Amaral, A. L., Nicolau, Ana, Coelho, M. A. Z., Ferreira, E. C.
Protozoa and metazoa are considered good indicators of the treatment quality in activated sludge systems due to the fact that these organisms are fairly sensitive to physical, chemical and...
An application of semi-infinite programming to air pollution control (2007)
Vaz, A. Ismael F., Ferreira, E. C.
Environment issues are more then ever important in a modern society. Complying with stricter legal thresholds on pollution emissions raises an important economic issue. This talk presents some ideas...
Production of fructooligosaccharides by aureobasidium sp. and aspergillus sp. (2007)
Dominguez, Ana, Nobre, C., Torres, D., Rocha, Orlando, Rodrigues, L. R., Rocha, I., ...
Exact fuzzy observer for a baker’s yeast fermentation process (2007)
Herrera, Enrique, Castillo, Bernardino, Ramírez, Jesús, Ferreira, E. C.
The purpose of this work is to design an exact fuzzy observer for a bioprocess switching between two different metabolic states. A continuous baker’s yeast culture is divided in two sub-models: a...
Rocha, Miguel, Pinto, José P., Rocha, I., Ferreira, E. C.
Although important contributions have been made in recent years within the field of bioprocess model development and validation, in many cases the utility of even relatively good models for process...
Ginoris, Y. P., Amaral, A. L., Nicolau, Ana, Coelho, M. A. Z., Ferreira, E. C.
A procedure for the semi-automatic identification of the main protozoa and metazoa species present in the activated sludge of wastewater treatment plants was developed. This procedure was based on...
Modelo exacto difuso de un cultivo por lote alimentado (2007)
Herrera, Enrique, Castillo, Bernardino, Ramírez, Jesús, Ferreira, E. C.
Mexican Consejo Nacional de Ciencia y Tecnología (CONACYT) - projectos 46538, 41148
Veloso, Ana C. A., Rocha, I., Ferreira, E. C.
In bioreactors, the measurement of variables that play a key role in the quality and productivity of fermentations, is of major importance. However, their direct measurement is often expensive or...
Takagi-Sugeno multiple-model controller for a continuous baking yeast fermentation process (2007)
Herrera, Enrique, Castillo, Bernardino, Ramírez, Jesús, Ferreira, E. C.
The purpose of this work is to design a fuzzy integral controller to force the switching of a bioprocess between two different metabolic states. A continuous baking yeast culture is divided in two...
Torres, D., Dominguez, Ana, Rodrigues, L. R., Rocha, I., Cardoso, Susana M., Silva, Artur M. S., ...
Agência de Inovação - Projecto Biolife, PRIME 03/347
Abreu, A. A., Costa, J. C., Araya-Kroff, P., Ferreira, E. C., Alves, M. M.
Due to unspecified operational problems, the specific acetoclastic activity (SAA) of the anaerobic granular sludge present in an industrial UASB reactor was considerably damaged (from 250 to less...
Wasted sludge treatment contributions in the WWTP total cost (2007)
This paper aims to analyze the cost contribution of a simple wasted sludge process in the WWTP design total cost. The plant design relies on a combined ATV and double exponential model to describe...
Optimization of bacterial strains with variable-sized evolutionary algorithms (2007)
Rocha, Miguel, Pinto, José P., Rocha, I., Ferreira, E. C.
In metabolic engineering it is difficult to identify which set of genetic manipulations will result in a microbial strain that achieves a desired production goal, due to the complexity of the...
Modeling and simulation of fructo-oligosaccharides production (2007)
Rocha, Orlando, Dominguez, Ana, Nobre, C., Torres, D., Rodrigues, L. R., Teixeira, J. A., ...
It is foreseen that Systems Biology will have a great impact not only in Metabolic Engineering and Drug Discovery efforts, but also in Bioprocess development and optimization. In fact, the...
Esteves, Ana Paula, Ferreira, E. C., Medeiros, Maria José
The controlled-potential reduction of [1-bromo-2-methoxy-2-(prop-2'-ynyloxy)ethyl]benzene(1a), 1-[2-bromo-2-phenyl-1-(prop-2'-ynyloxy)ethyl]-4-methoxybenzene (1b) and...
Rocha, Miguel, Pinto, José P., Rocha, I., Ferreira, E. C.
Although important contributions have been made in recent years within the field of bioprocess model development and validation, in many cases the utility of even relatively good models for process...
Ginoris, Y. P., Amaral, A. L., Nicolau, Ana, Coelho, M. A. Z., Ferreira, E. C.
Different protozoa and metazoa populations develop in the activated sludge wastewater treatment processes and are highly dependent on the operating conditions. In the current work the protozoa and...
A mathematical model for a Salmonella bacteriophage (2007)
Santos, Sílvio, Azeredo, Joana, Nicolau, Ana, Ferreira, E. C.
Ginoris, Y. P., Amaral, A. L., Ferreira, E. C., Coelho, M. A. Z., Nicolau, Ana
Os protozoários e pequenos metazoários são organismos abundantes nos tanques de aeração das Estações de Tratamento de Efluentes (ETEs) por lodos ativados. A distribuição de espécies e sua...
Metabolic modelling of stringent response in recombinant Escherichia coli (2007)
Carneiro, S., Ferreira, E. C., Rocha, I.
Recombinant protein production in Escherichia coli often derive cellular stress responses characterized by several biochemical reactions, most of which controlled at the genetic level. Drainage of...
Dias, A. M. A., Alves, M. M., Ferreira, E. C.
Computational intelligence models are being increasingly employed for the supervision and control of biological wastewater treatment systems. These models can be described as mathematical...
Purification of fructo-oligosaccharides by adsorption onto charcoal filters (2007)
Nobre, C., Dominguez, Ana, Torres, D., Rocha, Orlando, Rodrigues, L. R., Rocha, I., ...
Production of fructo-oligosaccharides has received particular attention recently due to its beneficial effects as prebiotics. Fermentative processes appear to be a good technique for large scale...
Costa, J. C., Alves, M. M., Ferreira, E. C.
Morphological parameters, obtained by quantitative image analysis techniques, together with physiological and reactor performance data were inserted in principal components analysis (PCA) to detect...
Morphology and physiology of anaerobic granular sludge exposed to organic solvents (2007)
Costa, J. C., Moita, I., Ferreira, E. C., Alves, M. M.
The use of quantitative image analysis techniques, together with physiological information might be used to monitor and detect operational problems in advance to reactor performance failure....
Evaluating simulated annealing algorithms in the optimization of bacterial strains (2007)
Rocha, Miguel, Mendes, Rui, Maia, Paulo, Pinto, José P., Rocha, I., Ferreira, E. C.
In this work, a Simulated Annealing (SA) algorithm is proposed for a Metabolic Engineering task: the optimization of the set of gene deletions to apply to a microbial strain to achieve a desired...
Saccharomyces cerevisiae morphology under hyperbaric gases (2007)
Coelho, M. A. Z., Coutinho, J. A. P., Mota, M., Ferreira, E. C., Belo, Isabel
The effects of hyperbaric stress on the morphology of Saccharomyces cerevisiae were studied in batch cultures under pressures between 0.1 MPa and 0.6 MPa and different gas compositions (air, O2, N2,...
Quantification of the CBD-FITC conjugates surface coating on cellulose fibres (2007)
Pinto, João Ricardo, Amaral, A. L., Ferreira, E. C., Mota, M., Vilanova, Manuel, Ruel, Katia, ...
Background Cellulose Binding Domains (CBD) were conjugated with fluorescein isothiocyanate (FITC). The surface concentration of the Binding Domains adsorbed on cellulose fibres was determined by...
Optimal trajectory approximation by cubic splines on fed-batch control problems (2006)
Vaz, A. Ismael F., Ferreira, E. C., Mota, Alzira
Optimal control problems appear in several engineering fields and in particular on the control of fedbatch fermentation processes. These problems are often described by sets of nonlinear differential...
Contribution of a simple sludge treatment in a WWTP optimization procedure (2006)
In the sequence of the work done with a combined ATV and double exponential model to describe the secondary settler concerning the optimal design of a wastewater treatment plant (WWTP), a simple...
Escherichia coli has been the organism of choice for the production of many recombinant proteins with high therapeutic value. However, while there have been several studies covering the recombinant...
Modelling bacteria and bacteriophage population dynamics (2006)
Santos, Sílvio, Azeredo, Joana, Nicolau, Ana, Ferreira, E. C.
Moreover, it is necessary to evaluate and to understand the phage and bacteria population dynamics in order to foresee its potential for use in vivo. To understand how natural communities are...
On the secondary settler Models robustness by simulation (2006)
The aim of this paper is to assess the goodness of the solutions obtained when minimizing the investment and operation costs of a wastewater treatment plant considering three secondary settler...
Determinação de trajectória óptima em processos de fermentação semi-contínua (2006)
Mota, Alzira, Vaz, A. Ismael F., Ferreira, E. C.
Uma grande parte de produtos valiosos são produzidos usando processos de fermentação e consequentemente a optimização destes processos reveste-se de uma grande importância económica. Em geral...
Rocha, I., Rocha, Miguel, Ferreira, E. C., Patil, Kiran Raosaheb, Nielsen, Jens
In metabolic engineering problems, due to the complexity of metabolic networks, it is often difficult to identify a priori which genetic manipulations will originate a given desired phenotype....
Efeito do sal no desempenho de um reator batelada sequencial (2006)
Mesquita, D. P., Coelho, M. A. Z., Ferreira, E. C.
Neste trabalho investigou-se o desempenho de um Reator Batelada Sequencial no tratamento de efluentes salinos (0,5 a 6,0%) utilizando uma estratégia de enchimento por pulsos simétricos....
Purification of fructo-oligosaccharides by nanofiltration (2006)
Nobre, C., Dominguez, Ana, Torres, D., Rocha, Orlando, Rodrigues, L. R., Rocha, I., ...
Ginoris, Y. P., Amaral, A. L., Nicolau, Ana, Ferreira, E. C., Coelho, M. A. Z.
A mixed culture of microorganisms is usually present in biological wastewater treatment processes such as the activated sludge system in aeration tanks. These microorganisms are capable of reducing...
Optimization and simulation of secondary settler models (2006)
This work focus on the accomplishing of the best model to a secondary settler to use in a minimum cost optimization procedure concerning the construction and operation of a wastewater treatment plant...
Costa, J. C., Abreu, A. A., Ferreira, E. C., Alves, M. M.
Two shock loads of a detergent (shock 1 with 0.15 gCOD/L during 56 h, and shock 2 with 0.30 gCOD/L during 222 h) were applied in a lab-scale EGSB reactor with 1.5 gCOD/L of ethanol. Quantitative...
Optimal control of fed-batch processes with particle swarm optimization (2006)
Vaz, A. Ismael F., Ferreira, E. C.
Optimal control problems appear in several engineering fields. These problems are often described by sets of nonlinear differential and algebraic equations, usually subject to constraints in the...
Design of estimators for specific growth rate control in a fed-batch E. coli fermentation (2006)
Rocha, I., Veloso, Ana C. A., Ferreira, E. C.
The specific growth rate is one of the most important process variables characterizing the state of microorganisms during fermentations mainly because the biosynthesis of many products of interest is...
Pires, O. C., Palma, C., Costa, J. C., Moita, I., Alves, M. M., Ferreira, E. C.
A supervisory expert system based on fuzzy logic rules was developed for diagnosis and control of a laboratory- scale plant comprising anaerobic digestion and anoxic/aerobic modules for combined high...
How wastewater processes can be optimized using LOQO (2006)
This paper describes the optimization af an activated sludge system wich comprises an aeration tank and a secondary settler. This system is by far the most widely used biological process in...
Optimização e controlo da poluição atmosférica com programação semi-infinita (2006)
Vaz, A. Ismael F., Ferreira, E. C.
As questões ambientais em geral e as da poluição atmosférica em particular têm merecido particular atenção por parte dos decisores. As fontes não móveis têm uma contribuição importante...
Amaral, A. L., Alves, M. M., Mota, M., Ferreira, E. C.
Image processing and analysis methodologies are considered nowadays as extremely useful tools in a wide range of applications. One of their main advantages resides on the ability to remove human...
A comparison of algorithms for the optimization of fermentation processes (2006)
Mendes, Rui, Rocha, I., Ferreira, E. C., Rocha, Miguel
The optimization of biotechnological processes is a complex problem that has been intensively studied in the past few years due to the economic impact of the products obtained from fermentations. In...
How wastewater processes can be optimized using LOQO (2006)
This paper describes the optimization of an activated sludge system which comprises an aeration tank and a secondary settler. This system is by far the most widely used biological process in...
Specific growth rate regulation in a simulated fed-batch E. coli fermentation (2006)
The specific growth rate is one of the most important process variables characterizing the state of microorganisms during fermentations mainly because the biosynthesis of many products of interest is...
Image analysis methodology to study the evolution of chromium penetration in chromium tannage (2006)
Amaral, A. L., Mota, M., Ferreira, E. C., Crispim, A.
The chrome tanning process is the main method employed to improve the hide stabilization, and chrome tanning in organic solvent medium has been tried by some authors, namely for ovine skins, but the...
Fed-batch trajectory optimal control problems with cubic splines (2006)
Vaz, A. Ismael F., Ferreira, E. C., Mota, Alzira
Optimal control problems in fed-batch fermentation processes are often described by sets of nonlinear differential and algebraic equations. The presence of constraints in the state and control...
Araya-Kroff, P., Amaral, A. L., Mota, M., Ferreira, E. C., Alves, M. M.
Cuatro reactores EGSB (R1-R4) fueron operados a velocidades de carga orgánica de 10 kgCOD/(m³.d), tiempos de retención hidráulicos de 3.6 h, velocidades superficiales de 10 m/h y diferentes...
Vivanco, E., Puñal, A., Pizarro, C., Ferreira, E. C., Alves, M. M., Chamy, Rolando
In this work the effect on granules morphology by adding a supplementary nitrogen-ammonium source to UASB and EGSB reactors, treating a low concentrated wastewater is studied. Previously, a...
A new representation in evolutionary algorithms for the optimization of bioprocesses (2005)
Rocha, Miguel, Rocha, I., Ferreira, E. C.
Evolutionary Algorithms (EAs) have been used to achieve optimal feedforward control in a number of fed-batch fermentation processes. Typically, the optimization purpose is to set the optimal feeding...
Assessment of yeast viability under hyperbaric conditions through a modeling approach (2005)
Coutinho, J. A. P., Belo, Isabel, Ferreira, E. C., Coelho, M. A. Z.
The effect of pressure (0.1–1.5 MPa) and oxygen concentration on the growth and viability of Saccharomyces cerevisiae was studied. Cell viability was assessed through the Methylene Blue staining...
Design of on-line state estimators for a recombinant E. coli fed-batch fermentation (2005)
Veloso, Ana C. A., Rocha, I., Ferreira, E. C.
In recent years a remarkable effort has been made in the development of new sensors and process analytical technology. However, it is still difficult to find reliable and low cost commercial sensors...
Biological process optimal design in a wastewater treatment plant (2005)
The aim of this paper is to determine the optimal design and operation of an activated sludge system that is being installed in a small town in the north of Portugal. This process design takes into...
A systems biology approach for the optimization of recombinant protein production in E. coli (2005)
Escherichia coli has been the organism of choice for the production of many recombinant proteins with high therapeutic value. However, while the research on molecular biology has allowed the...
Araújo, M. Alberta, Ferreira, E. C., Cunha, A. M., Mota, M.
Blends of corn starch with poly(ethylene-vinylalcohol) copolymer (SEVA-C) have been studied and reported as biodegradable. These materials are known to be sensitive to enzymatic action, evidencing a...
Veloso, Ana C. A., Rocha, I., Ferreira, E. C.
An optimal experimental design for yield coefficients estimation in an unstructured growth model of fed-batch fermentation of E. coli is presented. The feed profile is designed by optimisation of a...
Pires, O. C., Palma, C., Costa, J. C., Moita, I., Alves, M. M., Ferreira, E. C.
A supervisory expert system based on fuzzy logic rules was developed for diagnosis and control of a lab- scale plant comprising anaerobic/anoxic/aerobic modules for combined high rate biological N...
Amaral, A. L., Ferreira, E. C.
The biomass present in awastewater treatment plantwas surveyed and their morphological properties related with operating parameters such as the total suspended solids (TSS) and sludge volume index...
On-line estimation of biomass in an E. coli fed-batch fermentation (2005)
Veloso, Ana C. A., Rocha, I., Ferreira, E. C.
In this work, an Extended Kalman Observer is applied to the on-line determination of biomass concentration in a high-cell density fed-batch fermentation of Escherichia coli. Although the importance...
Pires, O. C., Palma, C., Moita, I., Costa, J. C., Alves, M. M., Ferreira, E. C.
A supervisory expert system based on fuzzy logic rules was developed for diagnosis and control of a lab- scale plant comprising anaerobic/anoxic/aerobic modules for combined high rate biological N...
Aplicações de técnicas de processamento e análise de imagem em processos biotecnológicos (2005)
Amaral, A. L., Alves, M. M., Mota, M., Ferreira, E. C.
Neste trabalho, são apresentados quatro estudos utilizando técnicas de processamento e análise de imagem e de estatística multivariável para monitorizar processos aeróbios e anaeróbios de...
Pacifico, Daniela Gerevini Pereira, Ferreira, E. C., Kilikian, Beatriz Vahan
Os pigmentos vermelhos produzidos por Monascus sp são substitutos de sais de nitrito (conservantes), os quais são precursores de nitrosamina (cancerígeno). O objetivo deste trabalho foi...
Determinação da concentração de CBD-FITC adsorvidos em fibras por análise de imagem (2005)
Amaral, A. L., Pinto, João Ricardo, Carvalho, Joana, Ferreira, E. C., Gama, F. M.
Neste trabalho, foi elaborado um programa de processamento e análise de imagem para a determinação da quantidade de CBD-FITC adsorvido em fibras de celulose. Este programa consiste em duas fases...
Motta, Maurício Da, Pons, M. N., Silva, Valdinete Lins Da, Andrade, Aline Clemente De, Amaral, A. L., Ferreira, E. C.
Neste trabalho é proposto um procedimento para reconhecimento semi-automático das espécies de protozoários por análise de imagem. Este programa (ProtoRec v.2) foi em seguida utilizado para...
Development of image analysis methods to evaluate barley / malt grain size (2005)
Amaral, A. L., Rocha, Orlando, Gonçalves, Cristina, Ferreira, António Augusto, Ferreira, E. C.
It is known that the barley / malt grain size is an important factor regarding the uniformity of malting process and hence the brewery process. For that purpose an image processing and analysis...
Estratégias de minimização de efluentes na síntese e integração de processos (2005)
No presente estudo avaliou-se a possibilidade de métodos de optimização na resolução de problemas de minimização de águas potáveis e efluentes. Utilizaram-se para o efeito diferentes...
Design of interval observers for an E. coli fed-batch fermentation with uncertain inputs (2005)
Veloso, Ana C. A., Rocha, I., Ferreira, E. C.
In bioreactors, the measurement of variables that play a key role in the quality and productivity of fermentations, is of major importance. However, their direct measurement is often expensive or...
Carneiro, S., Veloso, Ana C. A., Amaral, A. L., Rocha, I., Ferreira, E. C.
The development of monitoring methods for physiological state assessment during recombinant fermentation processes has been encouraged by the need to evaluate the influence of processing conditions...
Rocha, Miguel, Neves, José, Veloso, Ana C. A., Ferreira, E. C., Rocha, I.
In this work, Evolutionary Algorithms (EAs) are used to control a recombinant bacterial fed-batch fermentation process, that aims at producing a bio-pharmaceutical product. In a first stage, a novel...
NEOS server usage in wastewater treatment cost minimization (2005)
This paper describes the optimal design and operation of an activated sludge system in wastewater treatment plants. The optimization problem is represented as a smooth programming problem with linear...
Engenharia Química sem fronteiras : novas tendências no ensino da Engenharia Química (2005)
Apresentação efectuada no Colóquio anual de Engenharia Química - "Engenharia Química sem fronteiras", Universidade Federal do Rio de Janeiro, Rio de Janeiro.
Prebiotics : from concept to product : the BIOLIFE project (2005)
Torres, D., Dominguez, Ana, Rodrigues, L. R., Rocha, I., Teixeira, J. A., Lima, Nelson, ...
Nutrition has progressed from the discovery of essential nutrients and prevention of dietary deficiency to the promotion of a state of well-being and health and the reduction of the risk of disease....
Abreu, A. A., Costa, J. C., Araya-Kroff, P., Ferreira, E. C., Alves, M. M.
The specific acetoclastic activity (SAA) of anaerobic granular sludge was recovered in a lab scale EGSB reactor. The inoculum presented a very low acetoclastic activity (0.81+/-2.48mg...
Rocha, Miguel, Rocha, I., Ferreira, E. C., Veloso, Ana C. A.
In this work, Evolutionary Algorithms (EAs) were used to control a recombinant bacterial fed-batch fermentation process that aims to produce a biopharmaceutical product. Initially, a novel EA, based...
Veloso, Ana C. A., Rocha, I., Carvalho, S., Carneiro, S., Dias, T., Peres, A. M., ...
The ability to cultivate microbial strains, expressing heterologous recombinant proteins, is an increasingly important technique in biotechnology. Among the many systems available Escherichia coli...
Rocha, I., Carneiro, S., Veloso, Ana C. A., Ferreira, E. C.
Escherichia coli has been the organism of choice for the production of many recombinant proteins with high therapeutic value. However, while the research on molecular biology has allowed the...
Biological Process Optimal Design in a Wastewater Treatment Plant (2005)
The aim of this paper is to determine the optimal design and operation of an activated sludge system that is being installed in a small town in the north of Portugal. This process design takes into...
Optimização e controlo da poluição atmosférica (2004)
Vaz, A. Ismael F., Ferreira, E. C.
Alguns problemas de controlo da poluição atmosférica podem ser formulados como problemas de programação semi-infinita (PSI). Estas formulações, das quais descrevemos três abordagens, permitem...
Optimização de um processo biológico de tratamento de águas residuais (2004)
Neste artigo apresenta-se um problema de optimização relacionado com um processo biológico de tratamento de águas residuais. Neste problema pretende-se minimizar uma função custo de um sistema...
Air pollution control with semi-infinite programming (2004)
Vaz, A. Ismael F., Ferreira, E. C.
Air pollution control problems can be formulated as a semi-infinite programming (SIP) problem and we describe three main approaches. The first consists in optimizing an objective function while the...
Optimization of wastewater treatment processes (2004)
En este trabajo usamos el paquete de software LOQO para optimización de procesos de tratamiento de aguas residuales. Se minimizó una función costo, el volumen del reactor y el caudal de agua para...
During a high-cell density fed-batch fermentation of recombinant E. coli, both oxygen and carbon dioxide transfer rates (OTR and CTR) were calculated on-line from inlet and exhaust gas composition...
Amaral, A. L., Ferreira, E. C.
The wastewater treatment plant activated sludge is a complex ecosystem mainly of bacteria and protozoa. Bacteria agglomerate as settleable robust aggregates leading to low organic matter and...
Semi-infinite air pollution control problems (2004)
Vaz, A. Ismael F., Ferreira, E. C.
Semi-infinite programming (SIP) problems arise in many engineering areas. Robot trajectory planning and optimal signal sets are two fine examples. Air pollution abatement problems, which are linear...
Araya-Kroff, P., Amaral, A. L., Neves, L., Ferreira, E. C., Pons, M. N., Mota, M., ...
Image analysis techniques were developed and applied to quantify the process of anaerobic granulation in an expanded granular sludge blanket reactor (EGSB) fed with a synthetic substrate based on...
Amaral, A. L., Motta, Maurício Da, Pons, M. N., Vivier, H., Mota, M., Ferreira, E. C., ...
The biota present in the activated sludge of a wastewater treatment plant are mainly composed of bacteria, Protozoa and Metazoa. The Protozoa and Metazoa species are important micro-organisms taking...
Amaral, A. L., Pereira, M. A., Motta, Maurício Da, Pons, M. N., Mota, M., Ferreira, E. C., ...
Image analysis techniques are applied to monitor the morphological changes in granular sludge present in an expanded granular sludge blanket (EGSB) reactor fed with oleic acid. Deterioration of...
Effect of hyperbaric stress on yeast morphology : study by automated image analysis (2004)
Coelho, M. A. Z., Belo, Isabel, Pinheiro, Rita, Amaral, A. L., Mota, M., Coutinho, J. A. P., ...
The effects of hyperbaric stress on the morphology of Saccharomyces cerevisiae were studied in batch cultures under pressures between 0.1 MPa and 0.6 MPa and different gas compositions (air, oxygen,...
Ferreira, E. C., Lima, Ricardo, Salcedo, Romualdo
Recent developments in embedding numerical optimization procedures with linear and nonlinear solvers within a spreadsheet environment have greatly enhanced the use of these tools for teaching...
A genetic algorithm was used to estimate both yield and kinetic coefficients of an unstructured model representing a high-cell density fermentation of E. coli. The model is composed of mass balance...
Determination and modeling of yeast viability under stress conditions (2004)
Coutinho, J. A. P., Coelho, M. A. Z., Ferreira, E. C., Belo, Isabel
In this work the effect of the total pressure and the oxygen concentration in the gas phase on the growth and viability of Saccharomyces cerevisiae is studied. Cell viability was measured staining...
Alves, M. M., Pereira, M. A., Amaral, A. L., Pons, M. N., Mota, M., Ferreira, E. C.
Image analysis techniques are applied to monitor the morphological changes in granular sludge, present in an EGSB reactor fed with oleic acid. Deterioration of granular sludge was monitored along the...
Araya-Kroff, P., Amaral, A. L., Mota, M., Ferreira, E. C., Alves, M. M.
Four EGSB reactors (R1 to R4) were operated at an organic loading rate of 10 kgCOD/(m³.d), hydraulic retention time of 3.6 h, upflow velocity of 10 m/h and different influent nitrate concentrations...
Monitoring granular stability in an industrial UASB reactor treating brewery wastewater (2004)
Araya-Kroff, P., Amaral, A. L., Ferreira, E. C., Silva, P. M., Mota, M., Alves, M. M.
An industrial scale UASB reactor treating a brewery wastewater was monitored during several months, in order to assess the usefulness of a morphological parameter relating the filaments length to the...
Evolutionary algorithms for optimal control in fed-batch fermentation processes (2004)
Rocha, Miguel, Neves, José, Rocha, I., Ferreira, E. C.
In this work, Evolutionary Algorithms (EAs) are used to achieve optimal feedforward control in a recombinant bacterial fed-batch fermentation process, that aims at producing a bio-pharmaceutical...
How wastewater processes can be optimized using LOQO (2004)
Poster apresentado na "12th French-German-Spanish Conference on Optimization", Avignon, France, September 20-24, 2004.
Comparison of yeast response to increased pressure at aerobic and anaerobic conditions (2003)
Belo, Isabel, Ferreira, E. C., Pinheiro, Rita, Coutinho, J. A. P., Coelho, M. A. Z., Mota, M.
In this work, a genetic algorithm was used to estimate both yield and kinetic coefficients of an unstructured model representing a fed-batch high-cell density fermentation of E. coli. The model,...
Dias, Nicolina, Amaral, A. L., Ferreira, E. C., Lima, Nelson
Prova de tipográfica (In Press).
Motta, Maurício Da, Pons, M. N., Roche, N., Vivier, H., Amaral, A. L., Ferreira, E. C., ...
Um método baseado na análise de imagens foi desenvolvido para caracterizar os flocos de lodo ativado em termos do seu tamanho e forma (dimensão fractal) e da abundância de bactérias...
Pereira, M. A., Roest, K., Stams, A. J. M., Akkermans, A. D. L., Amaral, A. L., Pons, M. N., ...
Morphological changes in anaerobic granular sludge fed with increasing loads of oleic acid were quantified by image analysis. The combination of this technique with data on the accumulation of...
Lubenova, V., Rocha, I., Ferreira, E. C.
In this paper, an approach to the estimation of multiple biomass growth rates and biomass concentration is proposed for a class of aerobic bioprocesses characterized by on-line measurements of...
Kawasse, F. M., Amaral, P. F., Amaral, A. L., Ferreira, E. C., Coelho, M. A. Z.
Stress conditions (thermal and oxidative) were applied to Yarrowia lipolytica culture. A rearrangement in cell metabolism as well as dimorphism was observed under these conditions. An image analysis...
Lubenova, V., Rocha, I., Ferreira, E. C.
In this paper, a two step estimation approach is proposed for estimation of multiple (specific) growth rates and biomass concentration of fed-batch fermentation of recombinant E. coli. It is...
A model-based adaptive linearizing control law was derived for the regulation of the acetate concentration during the fed-batch fermentation of recombinant proteins with high cell density culture of...
Pereira, M. A., Roest, K., Stams, A. J. M., Akkermans, A. D. L., Amaral, A. L., Pons, M. N., ...
Morphological changes in anaerobic granular sludge fed with increasing loads of oleic acid were quantified by image analysis. The combination of this technique with data on the accumulation of...
Kawasse, F. M., Amaral, P. F., Coelho, M. A. Z., Amaral, A. L., Ferreira, E. C.
Yarrowia lipolytica is an aerobic microrganism capable to produce important metabolites, has an intense secretory activity which drives efforts to be employed in industry (as a biocatalyst), in...
Classification of Saccharomyces cerevisiae morphology employing image analysis (2002)
Coelho, M. A. Z., Amaral, A. L., Belo, Isabel, Mota, M., Coutinho, J. A. P., Ferreira, E. C.
Population dynamics of microbial systems can be described by several approaches and in various levels of complexity, each of them arising from specific goals and limitations. From the...
O ensino de engenharia biológica na Universidade do Minho (2002)
Ferreira, E. C., Teixeira, J. A.
Nos anos 80, a Biotecnologia foi considerada, a nível mundial, como uma das áreas prioritárias de desenvolvimento. Isto levou a que algumas universidades portuguesas começassem a oferecer alguma...
Two optimisation techniques for the fed-batch cultivation of high cell density Escherichia coli producing recombinant proteins were compared. An unstructured model for the growth, based on the...
A two-channel flow injection analysis (FIA) system was developed for the simultaneous on-line monitoring of acetate and glucose during high cell density fed-batch fermentations of recombinant...
Morphological Characterisation of Biomass in Wastewater Treatment Using Partial Least Squares (2002)
Stability, dynamics of convergence and tuning of observer-based kinetics estimators (2002)
Oliveira, Rui Manuel Freitas, Ferreira, E. C., Azevedo, S. Feyo De
This work discusses issues concerning stability, tuning and dynamics of convergence of observer-based kinetics estimators. The analysis focuses on both continuous and discrete time formulations of...
Motta, Maurício Da, Amaral, A. L., Neves, L., Araya-Kroff, P., Ferreira, E. C., Alves, M. M., ...
O estudo da biomassa de sistemas de tratamento de efluentes por análise de imagem permite avaliar o seu estado de agregação assim como acompanhar as modificações por ela sofrida quando submetida...
Image analysis as a tool to recognize anaerobic granulation time (2002)
Araya-Kroff, P., Amaral, A. L., Neves, L., Ferreira, E. C., Alves, M. M., Mota, M.
Image analysis techniques were used to monitor the development of anaerobic granules into an EGSB reactor fed with a synthetic substrate based on glucose (30-40% COD) and volatile fatty acids (70-60%...
Amaral, A. L., Pereira, M. A., Neves, L., Motta, Maurício Da, Pons, M. N., Vivier, H., ...
Suspended and granular sludge from two expanded granular sludge bed (EGSB) reactors fed with oleic acid at organic loads between 2 and 8 kg COD/m³.day was characterised by image analysis. The...
Ribeiro, Gabriela, Ferreira, E. C., Amorim, Cristina, Pereira, R. M. Álvares
O levantamento da situação de descarga de águas residuais industriais das empresas sediadas nos Parques Industriais de Adaúfe e Celeirós foi efectuado de Abril a Junho de 2000. A AGERE- E.M....
Faria, Rui, Costa, Cláudio, Ferreira, E. C.
Na ETAR de Serzedelo existiam problemas de separação de sólidos – foaming – provocados pelo crescimento excessivo de Nocardia spp, nas bacias de arejamento. Na tentativa de resolução deste...
"Bulking" filamentoso na ETAR de Braga : análise, diagnóstico e soluções (2002)
Rodrigues, Sofia, Amaral, A. L., Amorim, Cristina, Pereira, R. M. Álvares, Ferreira, E. C.
No tratamento secundário de águas residuais o processo de lamas activadas é o mais comunmente utilizado. Este assenta no crescimento de bactérias formadoras de flocos que se separa por gravidade...
Motta, Maurício Da, Amaral, A. L., Casellas, M., Pons, M. N., Dagot, C., Roche, N., ...
Automated methods based on the analysis of macro- and meso-scale images has been developed to characterise activated sludge in terms of size and shape (fractal dimension) of flocs and abundance of...
A system for the on-line monitoring of the major state variables during the production of recombinant proteins with E. coli was developed. Substrate (glucose) and the major by-product (acetate) were...
The study of protozoa population in wastewater treatment plants by image analysis (2001)
Motta,M.da, Pons,M.N., Vivier,H., Amaral,A.L., Ferreira,E.C., Roche,N., ...
Protozoa are important microorganisms for the ecosystem balance in wastewater treatment plants. A procedure for their semi-automated identification and counting based on image analysis is proposed....
Study of Protozoa population in wastewater treatment plants by image analysis (2001)
Motta, Maurício Da, Pons, M. N., Vivier, H., Amaral, A. L., Ferreira, E. C., Roche, N., ...
Protozoa are important microorganisms taking part to the ecosystem balance in wastewater treatment plants. A procedure for their semi-automated identification and counting based on image analysis is...
A Tool to Optimize VOC Removal During Absorption and Stripping (2001)
Ferreira, E. C., Salcedo, Romualdo
Practicing engineers routinely use modular simulators and algebraic tools to perform rigorous process optimizations. These complex tools, however, can have a significant learning curve. Meanwhile,...
Motta, Maurício Da, Amaral, A. L., Pons, M. N., Ferreira, E. C., Vivier, H., Mota, M.
A microfauna presente numa estação de tratamento de efluentes por lamas activadas é composta essencialmente por bactérias, protozoários e metazoários. Os protozoários apresentam uma...
Can Spreadsheet Solvers Solve Demanding Optimization Problems? (2001)
Ferreira, E. C., Salcedo, Romualdo
Practicing engineers resort to modular simulators or to algebraic tools such as GAMS or AMPL for performing complex process optimizations. These tools, however, have a significant learning curve...
Image Analysis and Multiphase Bioreactors (2001)
Ferreira, E. C., Mota, M., Pons, M. N.
The applications of visualisation and image analysis to bioreactors can be found in two main areas: the characterisation of biomass (fungi, bacteria, yeasts, animal and plant cells, etc), in terms of...
Ribeiro, N. J., Pires, O. C., Mota, M., Ferreira, E. C., Alves, M. M.
An upflow velocity of 0.21 m h−1 was optimal to minimize the effect of organic shocks (from 6 to 30 kg COD m−3 d−1) when operating an upflow anaerobic filter for the treatment of an oleic...
Motta, Maurício Da, Pons, M. N., Vivier, H., Roche, N., Amaral, A. L., Ferreira, E. C., ...
Une procédure a été développée pour la reconnaissance semi-automatique de protozoaires présents dans les boues activées. Après sélection sur l’image de la zone d’intérêt où se trouve...
Survey of a wastewater treatment plant microfauna by image analysis (2001)
Amaral, A. L., Motta, Maurício Da, Pons, M. N., Vivier, H., Mota, M., Ferreira, E. C.
The microfauna present in the activated sludge of a wastewater treatment plant is mainly composed by bacteria, protozoa and metazoa. The protozoan species are quite sensitive to physical, chemical...
Sludge characterization by image analysis (2001)
Motta, Maurício Da, Amaral, A. L., Ho, D., Pons, M. N., Vivier, H., Roche, N., ...
In wastewater treatment plants by activated sludge, a complex ecosystem constituted mainly of bacteria, protozoa and metazoa is degrading the pollution. Bacteria are agglomerated as flocs,...
Measurement of acetate during the production of recombinant proteins with E. coli (2000)
Acetate is known to be the major by-product during fermentation of recombinant E. coli. Among the consequences of that production, the more important are the decrease of the biomass yield for a given...
A system based on Flow Injection Analysis (FIA) for the on-line monitoring and data acquisition of acetate and glucose concentrations in the liquid phase during high cell density fed-batch...
Characterization of bubbles in a bubble column by image analysis (2000)
Freitas, Catarina, Amaral, A. L., Fialová, Maria, Ferreira, E. C., Zahradnik, Jindrich, Teixeira, J. A.
Fundação para a Ciência e a Tecnologia (FCT); EC.
Aplicação de um sistema de análise de imagem à monitorização da fermentação (2000)
Costa, A. M., Vicente, A. A., Amaral, A. L., Ferreira, E. C., Teixeira, J. A., Cruz, José Machado
Study of protozoa population in wastewater treatment plants by image analysis (2000)
Motta, Maurício Da, Pons, M. N., Vivier, H., Amaral, A. L., Ferreira, E. C., Roche, N., ...
Protozoa are important micro-organisms taking part to the ecosystem balance in wastewater treatment plants. A procedure for their semi-automated identification and counting based on image analysis is...
Perrier, M., Azevedo, S. Feyo De, Ferreira, E. C., Dochain, D.
This paper deals with the tuning of observer-based estimators. Initially, these algorithms were designed for estimating on-line kinetic parameters, like specific growth rates, in bioprocesses, and...
Alves, M. M., Ferreira, E. C., Amaral, A. L., Pereira, M. A., Novais, J. M., Mota, M.
This work describes a comparative study of staged and non-staged anaerobic filters for treating a synthetic dairy waste. The effect of decreasing the hydraulic retention time from 2 days to 10 h at a...
Characterisation by image analysis of anaerobic sludge under shock conditions (2000)
Alves, M. M., Cavaleiro, A. J., Ferreira, E. C., Amaral, A. L., Mota, M., Motta, Maurício Da, ...
In the present work the characterisation by image analysis of anaerobic biomass under organic and hydraulic shocks was performed. The digester was fed with a synthetic substrate, containing 50% oleic...
Baeza, J. A., Ferreira, E. C., Lafuente, J.
The hardware architecture and the software development of a real-time knowledge-based distributed control system for the supervision of a wastewater treatment pilot plant are presented. The operation...
Knowledge-based supervision and control of WWTP : a real-time implementation (2000)
Baeza, J. A., Ferreira, E. C., Lafuente, J.
Fundação Calouste Gulbenkian (FCG) - post-doctoral research grant.
Characterisation by image analysis of anaerobic microbial aggregates under shock conditions (2000)
Alves, M. M., Cavaleiro, A. J., Ferreira, E. C., Amaral, A. L., Mota, M., Motta, Maurício Da, ...
In the present work the characterisation by image analysis of anaerobic biomass under organic and hydraulic shocks was performed. The digester was fed with a synthetic substrate, containing 50% of...
Automated monitoring of activated sludge using image analysis (2000)
Motta, Maurício Da, Pons, M. N., Roche, N., Amaral, A. L., Ferreira, E. C., Alves, M. M., ...
An automated procedure for the characterisation by image analysis of the morphology of activated sludge has been used to monitor in a systematic manner the biomass in wastewater treatment plants....
An approach for multiple biomass growth rates and biomass concentration estimation is proposed for a class of bioprocesses characterizing by on-line measurements of dissolved oxygen concentration and...
Semi-automated recognition of protozoa by image analysis (1999)
Amaral, A. L., Baptiste, C., Pons, M. N., Nicolau, Ana, Lima, Nelson, Ferreira, E. C., ...
A programme was created to semi-automatically analyse protozoal digitised images. Principal Component Analysis technique was used for species identification. After data collection and mathematical...
Aplicações de técnicas de análise de imagem em microbiologia ambiental (1999)
Amaral, A. L., Mota, M., Ferreira, E. C.
São evidenciadas algumas aplicações da análise de imagem em microbiologia ambiental, nomeadamente: o reconhecimento e identificação automática de algumas espécies de protozoários presentes...
Analyse des flocs bactériens et de la microfaune des boues activées para analyse d'Image (1999)
Motta, Maurício Da, Pons, M. N., Roche, N., Amaral, A. L., Ferreira, E. C., Mota, M.
Brasil. Ministério da Ciência e Tecnologia. Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq).
Application of image analysis techniques in biotechnology (1998)
Image analysis is commonly used nowadays in a wide range of applications due to the development of faster computers, advanced frame grabbers, and sophisticated software. Although the availability of...
Automated characterization of protozoa in activated sludge (1998)
Baptiste, C., Amaral, A. L., Nicolau, Ana, Pons, M. N., Lima, Nelson, Ferreira, E. C., ...
BIOTEC’98 : book of abstracts (1998)
IV Iberian Congress on Biotechnology; I Ibero-American Meeting on Biotechnology
Mass transfer properties of glucose and O2 in Saccharomyces cerevisiae flocs (1998)
Vicente, A. A., Dluhý, M., Ferreira, E. C., Mota, M., Teixeira, J. A.
The use of flocculent microorganisms in fermentations is a very promising application. However, it has some drawbacks of which the most important ones are related to mass transfer limitations within...
Modelling diffusion-reaction phenomena in yeast flocs of Saccharomyces cerevisiae (1998)
Vicente, A. A., Dluhý, M., Ferreira, E. C., Teixeira, J. A.
Limitations in the diffusion of substrates into the flocs will condition cell metabolic behaviour, having obvious consequences on growth and product formation. Polymeric additives have been used...
Fontes de Informação para Engenharia na Internet (1998)
No presente artigo pretende-se abordar a utilização da Internet como veículo para a procura e divulgação de informação científica e técnica para os engenheiros. São apresentados os...
Baeza, J. A., Ferreira, E. C., Lafuente, J.
This paper presents the hardware architecture and the software development of a real-time knowledge-based distributed control system for the supervision of a wastewater treatment pilot plant with...
Real-time expert control of a pilot WWTP with nitrogen and phosphorus removal (1998)
Baeza, J. A., Ferreira, E. C., Lafuente, J.
Fundação Calouste Gulbenkian; C.I.R.I.T. (Generalitat de Catalunya).
Amaral, A. L., Nicolau, Ana, Ferreira, E. C., Lima, Nelson, Mota, M.
PRAXIS XXI.
Gabriel, D., Ferreira, E. C., Valero, F., Lafuente, J.
Fundação Calouste Gulbenkian.
Fontes de informação na internet para controlo e automação (1997)
No presente artigo pretende abordar-se a utilização da internet como veículo para a procura e divulgação de informação científica e técnica. São apresentados os principais serviços da...
Morphological characterisation of microbial aggregates by image analysis (1997)
Amaral, A. L., Alves, M. M., Mota, M., Ferreira, E. C.
A program, was accomplished in MATLAB, that allows the morphological characterisation of particles with special emphasis to microbial aggregates. After automatic identification and isolation of...
A new adaptive scheme for the adaptive linearizing control of bioprocesses (1996)
Ferreira, E. C., Azevedo, S. Feyo De
This work deals with the development of model-based adaptive control algorithms for bioprocess operation. Non-linear adaptive control laws are proposed for single input single output regulation....
Adaptive linearizing control of bioreactors (1996)
Ferreira, E. C., Azevedo, S. Feyo De
In this communication we shall present developments concerning the synthesis of Single-Input, Single-Output (SISO) and Multi-Input, Multi-Output (MIMO) adaptive linearizing algorithms for the...
A Internet como fonte de informação para engenharia (1996)
Versão internet em: http://www.deb.uminho.pt/ecferreira/ordem.htm
A study on the convergence of observer-based kinetics estimators in stirred tank bioreactors (1996)
Oliveira, Rui Manuel Freitas, Ferreira, E. C., Oliveira, Filomena, Azevedo, S. Feyo De
This paper is devoted to the tuning problem of an observer-based algorithm for the on-line estimation of reaction rates in stirred tank bioreactors. The relation between the dynamics of convergence...
Computer-based studies on bioprocess engineering : II - Tools for process operation (1996)
Azevedo, S. Feyo De, Pimenta, Pedro C. C., Oliveira, Filomena, Ferreira, E. C.
In this paper we review recent advances on the practice and theory of process control with particular emphasis to the operation of bioreactors. We present in detail a case-study on the modelling,...
Model-based estimation of reaction rates in stirred tank bioreactors (1996)
Oliveira, Rui Manuel Freitas, Ferreira, E. C., Azevedo, S. Feyo De
In this paper an adaptive model-based algorithm is proposed for the on-line estimation of reaction rates in stirred tank bioreactors. The main design condition imposes that the observation errors...
Vicente, A. A., Ferreira, E. C., Teixeira, J. A.
Flocculating cultures are one of the most interesting technologies to immobilise cells inside bioreactors. However, little is known about cell metabolism, viability and fermentation kinetics and...
Human capital and mobility network in chemical process control : second General Meeting (1996)
Azevedo, S. Feyo De, Ferreira, E. C.
Comissão Europeia - Contract n.º ERB-CHRX-CT94-0672.
Rocha, Cristina, Ferreira, E. C.
The main objective of this work is to elaborate methodologies that allow the identification of yield coefficients through complete measurements of the state. Experimental design strategies are...
Oliveira, Filomena, Ferreira, E. C., Azevedo, S. Feyo De
O presente trabalho descreve a integração de técnicas de controlo automático em processos biotecnológicos. Fazse uma análise dos requisitos específicos que uma instalação experimental...
A study on the convergence of observer-based kinetics estimators in stirred tank bioreactors (1994)
Oliveira, Rui Manuel Freitas, Ferreira, E. C., Oliveira, Filomena, Azevedo, S. Feyo De
This paper is devoted to the tuning problem of the "observer-based kinetics estimator" in stirred tank bioreactors. This algorithm estimates the reaction kinetics from the on-line knowledge of the...
Ferreira, E. C., Oliveira, Filomena, Pimenta, Pedro C. C., Oliveira, Rui Manuel Freitas, Azevedo, S. Feyo De
In this paper we present a simple computer-based laboratory set-up for experiments concerning real-time system identification. The set-up is constituted by a 'Process Simulator' computer and a...
Model-based identification and control on baker's yeast fed-batch fermentation (1993)
Ferreira, E. C., Oliveira, Filomena, Pimenta, Pedro C. C., Azevedo, S. Feyo De
A theoretical and experimental study is reported concerning the control of baker's yeast fed-batch fermentation. The overall approach follows the methodology proposed by Bastin & Dochain (1990) which...
Studies on on-line state and parameter estimation through a real-time process simulator (1992)
Azevedo, S. Feyo De, Pimenta, Pedro C. C., Oliveira, Filomena, Ferreira, E. C.
A PC-based simulator of non-linear multiple-input multiple-output dynamic systems was employed for studies concerning the on-line estimation of both 'non-measured' state variables and multiple...