Darilek, R. E., Dewar, J. A., Gould, G. P., Harris, E. D.
This study evaluated the U.S. Air Forces's survey of emerging technologies of the future, Project Forecast II, for its relevance to the Army's potential; requirements for the future, as indicated by...
Copper-induced activation of aortic lysyl oxidase in vivo.
Raising day-old chicks on diets lacking copper severely depressed the activity of lysyl oxidase, a copper metalloenzyme in connective tissue. Administration of CuSO4 either through the diet or...
Activation in vitro of rheumatoid synovial collagenase from cell cultures.
Vater, C A, Mainardi, C L, Harris, E D
Rheumatoid synovial cells dissociated from matrix and adherent to culture dishes released a latent form of collagenase into culture medium. Previous studies have shown that the latent enzyme does not...
34-year-old woman with hypotension, respiratory failure, fever, and an abdominal mass.
Lee, P C, Gocke, C D, Harris, E D, Pillsbury, H E, Bergin, C J, Vosti, K L, ...
Okada, Y, Harris, E D, Nagase, H
Two active forms (Mr 45,000 and 28,000) of a metalloendopeptidase that digest proteoglycans and other extracellular matrix components of connective tissues have previously been purified from...
Proactivator-dependent activation of procollagenase induced by treatment with EGTA.
Vater, C A, Nagase, H, Harris, E D
A new mechanism for activation of the proactivator of procollagenase [Vater, Nagase & Harris (1983) J. Biol. Chem. 258, 9374-9382] has been found. Collagenolytic and other proteolytic enzyme...
Regulation of aortic CuZn-superoxide dismutase with copper. Effects in vivo.
Cu2Zn2-superoxide dismutase (CuZn-SOD) was purified from chicken liver. The liver enzyme had a subunit Mr of 16900 and contained equimolar amounts of copper and zinc [0.26% (w/w) for each]. Aortic...
Caeruloplasmin and albumin were compared as potential donors of copper to Cu2Zn2-superoxide dismutase (CuZn-SOD) in culture. Aortas from 15-day copper-deficient chicks were suspended in oxygenated,...
K562 cells, a human erythroleukaemic cell line blocked for differentiation, commit towards erythrocytes when exposed to haemin (20 microM). The cells synthesize fetal haemoglobins and show...
Nagase, H, Brinckerhoff, C E, Vater, C A, Harris, E D
Monolayer cultures of rabbit synovial fibroblasts stimulated with phorbol myristate acetate to produce large amounts of collagenase (EC 3.4.24.7) were used to study the biosynthesis and secretion of...
Balthrop, J E, Dameron, C T, Harris, E D
Soluble fractions from chick liver and aorta were examined for copper-binding proteins. In liver a zinc-binding thionein appeared to be the major binding protein for copper. Aortic tissue contained...
Native cross-links in collagen fibrils induce resistance to human synovial collagenase.
Vater, C A, Harris, E D, Siegel, R C
A model system consisting of highly purified lysyl oxidase and reconstituted lathyritic chick bone collagen fibrils was used to study the effect of collagen cross-linking on collagen degradation by...
Purification and characterization of a collagenase extracted from rabbit tumours.
McCroskery, P A, Richards, J F, Harris, E D
A collagenase was purified from homogenates of V2 ascites-cell carcinoma growing in rabbit muscle. (NH4)2SO4 precipitation, ion-exchange and gel-filtration chromatography, and affinity chromatography...
Multiple transcripts coding for the menkes gene: evidence for alternative splicing of Menkes mRNA.
We isolated cDNA fragments from four human cell lines that had sequences for the Menkes Cu-transporting ATPase (ATP7A). Primers designed to generate a 4.8 kb cDNA with the complete open reading frame...
Evidence for a Menkes-like protein with a nuclear targeting sequence.
Reddy, M C, Majumdar, S, Harris, E D
Extracts from three human cell lines were found to contain abridged Menkes disease gene transcripts with novel insertion sequences. The transcript variant that is the focus of the present study codes...
Copper-induced activation of aortic lysyl oxidase in vivo.
Raising day-old chicks on diets lacking copper severely depressed the activity of lysyl oxidase, a copper metalloenzyme in connective tissue. Administration of CuSO4 either through the diet or...
Activation in vitro of rheumatoid synovial collagenase from cell cultures.
Vater, C A, Mainardi, C L, Harris, E D
Rheumatoid synovial cells dissociated from matrix and adherent to culture dishes released a latent form of collagenase into culture medium. Previous studies have shown that the latent enzyme does not...
34-year-old woman with hypotension, respiratory failure, fever, and an abdominal mass.
Lee, P C, Gocke, C D, Harris, E D, Pillsbury, H E, Bergin, C J, Vosti, K L, ...
Okada, Y, Harris, E D, Nagase, H
Two active forms (Mr 45,000 and 28,000) of a metalloendopeptidase that digest proteoglycans and other extracellular matrix components of connective tissues have previously been purified from...
Proactivator-dependent activation of procollagenase induced by treatment with EGTA.
Vater, C A, Nagase, H, Harris, E D
A new mechanism for activation of the proactivator of procollagenase [Vater, Nagase & Harris (1983) J. Biol. Chem. 258, 9374-9382] has been found. Collagenolytic and other proteolytic enzyme...
Regulation of aortic CuZn-superoxide dismutase with copper. Effects in vivo.
Cu2Zn2-superoxide dismutase (CuZn-SOD) was purified from chicken liver. The liver enzyme had a subunit Mr of 16900 and contained equimolar amounts of copper and zinc [0.26% (w/w) for each]. Aortic...
Caeruloplasmin and albumin were compared as potential donors of copper to Cu2Zn2-superoxide dismutase (CuZn-SOD) in culture. Aortas from 15-day copper-deficient chicks were suspended in oxygenated,...
K562 cells, a human erythroleukaemic cell line blocked for differentiation, commit towards erythrocytes when exposed to haemin (20 microM). The cells synthesize fetal haemoglobins and show...
Nagase, H, Brinckerhoff, C E, Vater, C A, Harris, E D
Monolayer cultures of rabbit synovial fibroblasts stimulated with phorbol myristate acetate to produce large amounts of collagenase (EC 3.4.24.7) were used to study the biosynthesis and secretion of...
Balthrop, J E, Dameron, C T, Harris, E D
Soluble fractions from chick liver and aorta were examined for copper-binding proteins. In liver a zinc-binding thionein appeared to be the major binding protein for copper. Aortic tissue contained...
Native cross-links in collagen fibrils induce resistance to human synovial collagenase.
Vater, C A, Harris, E D, Siegel, R C
A model system consisting of highly purified lysyl oxidase and reconstituted lathyritic chick bone collagen fibrils was used to study the effect of collagen cross-linking on collagen degradation by...
Purification and characterization of a collagenase extracted from rabbit tumours.
McCroskery, P A, Richards, J F, Harris, E D
A collagenase was purified from homogenates of V2 ascites-cell carcinoma growing in rabbit muscle. (NH4)2SO4 precipitation, ion-exchange and gel-filtration chromatography, and affinity chromatography...
Multiple transcripts coding for the menkes gene: evidence for alternative splicing of Menkes mRNA.
We isolated cDNA fragments from four human cell lines that had sequences for the Menkes Cu-transporting ATPase (ATP7A). Primers designed to generate a 4.8 kb cDNA with the complete open reading frame...
Evidence for a Menkes-like protein with a nuclear targeting sequence.
Reddy, M C, Majumdar, S, Harris, E D
Extracts from three human cell lines were found to contain abridged Menkes disease gene transcripts with novel insertion sequences. The transcript variant that is the focus of the present study codes...
Woolley, D E, Brinckerhoff, C E, Mainardi, C L, Vater, C A, Evanson, J M, Harris, E D
The dendritic cells of dissociated, adherent rheumatoid synovial cell cultures are recognised by their distinctive morphological features--compact cytoplasm around the nucleus and long, branched...
Survival of rheumatoid synovium implanted into nude mice.
Brinckerhoff, C. E., Harris, E. D.
The gross morphologic features, histologic features, and synthesis of collagenase and prostaglandin E2 by rheumatoid synovial cells injected into athymic nude mice were studied. A single cell...