E. de Vroom

Synthesis of cyclic oligonucleotides by a modified phosphotriester approach (1988)

De Vroom, E., Broxterman, H.J.G., Van Der Marel, G.A., Van Boom, J.H.

Evidence will be presented to show that the allyl group is suitable for the protection of a 3′-terminal phosphodiester function. The latter will be demonstrated by the synthesis, via a...

Synthesis of nuclelc acid methylphosphonothloates (1988)

De Vroom, E., Van Der Marel, G.A., Van Boom, J.H.

The reagent obtained in situ by treating methylphoaphonothioic dichlo-ride with l-hydroxy-6-trifluoromethylbenzotriazole could be used for the introduction of methylphoaphonothloate linkages. The...

Preparation of covalently linked DNA-RNA hybrids and arabinocytidine containing DNA fragments (1988)

De Vroom, E., Saris, C.P., Budding, T.N.W., Van Der Marel, G.A., Van Boom, J.H.

It will be demonstrated that 5′-O-DMT-N-acyl-deoxyribonucleosides, 5′- O-Lev-2′-O-MTHP-N-acyl-ribonucleosidea and, also, 2′-O-MTHP-N-acyl-ara-cyti-dine can be coupled, via the...

Preparation of the individual diastereomers of adenylyl-(2'-5')-P-thioadenylyl-(2'-5')-adenosine and their 5'-phosphorylated derivatives (1987)

De Vroom, E., Fidder, A., Saris, C.P., Van Der Marel, G.A., Van Boom, J.H.

The individual diastereomers of trimer A2′ p5′ A2′p(s)5′A, containing one phosphorothioate linkage, were prepared via a modified hydroxybenzotriazole phosphotriester approach. The...

Use of a 1- hydrorybenzotriazole activated pbospborylating reagent towards the synthesis of short RNA fragments in solution (1986)

De Vroom, E., Fidder, A., Marugg, J.E., Boom, Van J.H.

Evidence will be presented to show that the reagents 3a-c (X=0) obtained by the reaction of 2-chlorophenylphosphorodichloridate with 1- hydroxybenzotri-azole or...