Boluyt, M. O., Bing, O. H. L., Lakatta, E. G.
Spontaneously hypertensive rats (SHR) of advanced age exhibit depressed myocardial contractile function and ventricular fibrosis, as stable compensated hypertrophy progresses to heart failure....
Age associated changes in membrane currents in rat ventricular myocytes (1993)
Walker, K E, Lakatta, E G, Houser, S R
Objective: Action potential duration of senescent rat ventricular myocytes is longer than in young adults. The aim of the study was to determine whether age related changes in L-type calcium current...
Changes in cardiovascular function with aging (1990)
Overall cardiovascular function at rest in most healthy elderly individuals is adequate to meet the body's need for pressure and flow. The resting heart rate is unchanged. Heart size is essentially...
Similar myocardial effects of aging and hypertension (1990)
Aging has sometimes been referred to as ‘blunted hypertension’, and hypertension as ‘accelerated aging’. Systolic arterial pressure increases with aging; the increase encompasses both the...
FRANZ, M. R., BURKHOFF, D., SPURGEON, H., WEISFELDT, M. L., LAKATTA, E. G.
Monophasic action potential (MAP) recording with non-suction, ‘contact’ electrode catheters has been shown possible and safe during clinical catheterization, but direct validation of this new...
Xiao, R P, Cheng, H, Lederer, W J, Suzuki, T, Lakatta, E G
Calcium entry through voltage-gated Ca2+ channels is critical in cardiac excitation-contraction coupling and calcium metabolism. In this report, we demonstrate both spatially resolved and temporally...
Bilato, C, Pauly, R R, Melillo, G, Monticone, R, Gorelick-Feldman, D, Gluzband, Y A, ...
Intracellular signaling pathways activated by both PDGF and basic fibroblast growth factor (bFGF) have been implicated in the migration of vascular smooth muscle cells (VSMC), a key step in the...
Stern, M D, Silverman, H S, Houser, S R, Josephson, R A, Capogrossi, M C, Nichols, C G, ...
Anoxia of the heart causes failure of contraction before any irreversible injury occurs; the mechanism by which anoxia blocks cardiac excitation-contraction coupling is unknown. Studies in whole...
Age-associated changes in beta-adrenergic modulation on rat cardiac excitation-contraction coupling.
Xiao, R P, Spurgeon, H A, O'Connor, F, Lakatta, E G
Previous studies have demonstrated that the ability of beta-adrenergic receptor (beta AR) stimulation to increase cardiac contractility declines with aging. In the present study, the control...
Taxol inhibits neointimal smooth muscle cell accumulation after angioplasty in the rat.
Sollott, S J, Cheng, L, Pauly, R R, Jenkins, G M, Monticone, R E, Kuzuya, M, ...
Despite significant improvements in the primary success rate of the medical and surgical treatments for atherosclerotic disease, including angioplasty, bypass grafting, and endarterectomy, secondary...
Weiss, R G, Gerstenblith, G, Lakatta, E G
Delayed recovery of contractile function after myocardial ischemia may be due to prolonged recovery of high-energy phosphates, persistent acidosis, increased inorganic phosphate, and/or calcium...
Moderate alcohol intoxication in man, a ubiqitious social event, causes acute but reversible myocardial depression, the mechanism of which is unknown. We investigated whether this depression could be...
Prolonged contraction duration in aged myocardium.
Lakatta, E G, Gerstenblith, G, Angell, C S, Shock, N W, Weisfeldt, M L
Isometric performance at 29degreesC was measured in left ventricular trabeculae carneae from young adult (6-mo) and aged (25-mo) rats (n equals 18 in each group). Active tension and maximal rate of...
Wier, W G, Kort, A A, Stern, M D, Lakatta, E G, Marban, E
Indirect evidence suggests that fluctuations, or oscillations, in the intracellular free calcium concentration [( Ca2+]i) can occur spontaneously in intact cardiac preparations, but such [Ca2+]i...
p53 and the hypoxia-induced apoptosis of cultured neonatal rat cardiac myocytes.
Long, X, Boluyt, M O, Hipolito, M L, Lundberg, M S, Zheng, J S, O'Neill, L, ...
Myocyte cell loss is a prominent and important pathogenic feature of cardiac ischemia. We have used cultured neonatal rat cardiac myocytes exposed to prolonged hypoxia as an experimental system to...
Xiao, R P, Tomhave, E D, Wang, D J, Ji, X, Boluyt, M O, Cheng, H, ...
While an age-associated diminution in myocardial contractile response to beta-adrenergic receptor (beta-AR) stimulation has been widely demonstrated to occur in the context of increased levels of...
Long, X, Crow, M T, Sollott, S J, O'Neill, L, Menees, D S, ...
Activation of the vacuolar proton ATPase (VPATPase) has been implicated in the prevention of apoptosis in neutrophils and adult cardiac myocytes. To determine the role of the VPATPase in apoptosis of...
Cheng, H, Song, L S, Shirokova, N, González, A, Lakatta, E G, Ríos, E, ...
Determination of the calcium spark amplitude distribution is of critical importance for understanding the nature of elementary calcium release events in striated muscle. In the present study we show,...
G(i)-dependent localization of beta(2)-adrenergic receptor signaling to L-type Ca(2+) channels.
Chen-Izu, Y, Xiao, R P, Izu, L T, Cheng, H, Kuschel, M, Spurgeon, H, ...
A plausible determinant of the specificity of receptor signaling is the cellular compartment over which the signal is broadcast. In rat heart, stimulation of beta(1)-adrenergic receptor (beta(1)-AR),...
Ion concentration-dependence of rat cardiac unitary L-type calcium channel conductance.
Guia, A, Stern, M D, Lakatta, E G, Josephson, I R
Little is known about the native properties of unitary cardiac L-type calcium currents (i(Ca)) measured with physiological calcium (Ca) ion concentration, and their role in excitation-contraction...
1. Intracellular [Ca2+] ([Ca2+]i) transients, monitored by the fluorescent Ca2+ indicator, indo-1, and twitch contractions elicited by action potentials, by voltage clamp pulses or by rapid, brief...
The immunophilin FK506-binding protein modulates Ca2+ release channel closure in rat heart.
Xiao, R P, Valdivia, H H, Bogdanov, K, Valdivia, C, Lakatta, E G, Cheng, H
1. The nature of the signal that terminates the release of Ca2+ from the cardiac sarcoplasmic reticulum has remained elusive. This study was intended to examine whether FK506-binding protein (FKBP),...
Song, L S, Stern, M D, Lakatta, E G, Cheng, H
1. The exact nature of calcium sparks in the heart remains highly controversial. We sought to determine whether calcium sparks arise from a single or multiple calcium release channels/ ryanodine...
Spurgeon, H A, DuBell, W H, Stern, M D, Sollott, S J, Ziman, B D, Silverman, H S, ...
1. Single isolated rat cardiac myocytes were loaded with either the pentapotassium salt form or the acetoxymethyl ester (AM) form of the calcium-sensitive fluorescent probe, Indo-1. The relationship...
The cytosolic calcium transient modulates the action potential of rat ventricular myocytes.
DuBell, W H, Boyett, M R, Spurgeon, H A, Talo, A, Stern, M D, Lakatta, E G
1. The modulation of the action potential by the cytosolic Ca2+ (Cai2+) transient was studied in single isolated rat ventricular myocytes loaded with the acetoxymethyl ester form of the...
Ryanodine releases calcium from sarcoplasmic reticulum in calcium-tolerant rat cardiac myocytes.
1. The hypothesis tested in this study is that ryanodine depletes sarcoplasmic reticulum (s.r.) Ca2+ loading in suspensions of single adult rat cardiac myocytes by effecting Ca2+ release into the...
Kort, A A, Lakatta, E G, Marban, E, Stern, M D, Wier, W G
Ca2+-activated aequorin luminescence and tension were measured in dog Purkinje fibres during twitches and during the increase in resting force produced by exposure of the fibres to a low-Na+ solution...
Diastolic scattered light fluctuation, resting force and twitch force in mammalian cardiac muscle
1. When coherent light was passed through isolated isometric cardiac muscles during the diastolic or resting period, intensity fluctuations were observed in the scattered field. The frequency of...
Force staircase kinetics in mammalian cardiac muscle: modulation by muscle length
Lakatta, E. G., Spurgeon, H. A.
1. Quiescent cat papillary muscles were stimulated to contract regularly at Lmax, the length at which force production is optimal, and at 0·85 Lmax. The resulting increase in force production (rate...
Xiao, R P, Cheng, H, Lederer, W J, Suzuki, T, Lakatta, E G
Calcium entry through voltage-gated Ca2+ channels is critical in cardiac excitation-contraction coupling and calcium metabolism. In this report, we demonstrate both spatially resolved and temporally...
Bilato, C, Pauly, R R, Melillo, G, Monticone, R, Gorelick-Feldman, D, Gluzband, Y A, ...
Intracellular signaling pathways activated by both PDGF and basic fibroblast growth factor (bFGF) have been implicated in the migration of vascular smooth muscle cells (VSMC), a key step in the...
Stern, M D, Silverman, H S, Houser, S R, Josephson, R A, Capogrossi, M C, Nichols, C G, ...
Anoxia of the heart causes failure of contraction before any irreversible injury occurs; the mechanism by which anoxia blocks cardiac excitation-contraction coupling is unknown. Studies in whole...
Age-associated changes in beta-adrenergic modulation on rat cardiac excitation-contraction coupling.
Xiao, R P, Spurgeon, H A, O'Connor, F, Lakatta, E G
Previous studies have demonstrated that the ability of beta-adrenergic receptor (beta AR) stimulation to increase cardiac contractility declines with aging. In the present study, the control...
Taxol inhibits neointimal smooth muscle cell accumulation after angioplasty in the rat.
Sollott, S J, Cheng, L, Pauly, R R, Jenkins, G M, Monticone, R E, Kuzuya, M, ...
Despite significant improvements in the primary success rate of the medical and surgical treatments for atherosclerotic disease, including angioplasty, bypass grafting, and endarterectomy, secondary...
Weiss, R G, Gerstenblith, G, Lakatta, E G
Delayed recovery of contractile function after myocardial ischemia may be due to prolonged recovery of high-energy phosphates, persistent acidosis, increased inorganic phosphate, and/or calcium...
Moderate alcohol intoxication in man, a ubiqitious social event, causes acute but reversible myocardial depression, the mechanism of which is unknown. We investigated whether this depression could be...
Prolonged contraction duration in aged myocardium.
Lakatta, E G, Gerstenblith, G, Angell, C S, Shock, N W, Weisfeldt, M L
Isometric performance at 29degreesC was measured in left ventricular trabeculae carneae from young adult (6-mo) and aged (25-mo) rats (n equals 18 in each group). Active tension and maximal rate of...
Wier, W G, Kort, A A, Stern, M D, Lakatta, E G, Marban, E
Indirect evidence suggests that fluctuations, or oscillations, in the intracellular free calcium concentration [( Ca2+]i) can occur spontaneously in intact cardiac preparations, but such [Ca2+]i...
p53 and the hypoxia-induced apoptosis of cultured neonatal rat cardiac myocytes.
Long, X, Boluyt, M O, Hipolito, M L, Lundberg, M S, Zheng, J S, O'Neill, L, ...
Myocyte cell loss is a prominent and important pathogenic feature of cardiac ischemia. We have used cultured neonatal rat cardiac myocytes exposed to prolonged hypoxia as an experimental system to...
Xiao, R P, Tomhave, E D, Wang, D J, Ji, X, Boluyt, M O, Cheng, H, ...
While an age-associated diminution in myocardial contractile response to beta-adrenergic receptor (beta-AR) stimulation has been widely demonstrated to occur in the context of increased levels of...
Long, X, Crow, M T, Sollott, S J, O'Neill, L, Menees, D S, ...
Activation of the vacuolar proton ATPase (VPATPase) has been implicated in the prevention of apoptosis in neutrophils and adult cardiac myocytes. To determine the role of the VPATPase in apoptosis of...
1. Intracellular [Ca2+] ([Ca2+]i) transients, monitored by the fluorescent Ca2+ indicator, indo-1, and twitch contractions elicited by action potentials, by voltage clamp pulses or by rapid, brief...
The immunophilin FK506-binding protein modulates Ca2+ release channel closure in rat heart.
Xiao, R P, Valdivia, H H, Bogdanov, K, Valdivia, C, Lakatta, E G, Cheng, H
1. The nature of the signal that terminates the release of Ca2+ from the cardiac sarcoplasmic reticulum has remained elusive. This study was intended to examine whether FK506-binding protein (FKBP),...
Song, L S, Stern, M D, Lakatta, E G, Cheng, H
1. The exact nature of calcium sparks in the heart remains highly controversial. We sought to determine whether calcium sparks arise from a single or multiple calcium release channels/ ryanodine...
Spurgeon, H A, DuBell, W H, Stern, M D, Sollott, S J, Ziman, B D, Silverman, H S, ...
1. Single isolated rat cardiac myocytes were loaded with either the pentapotassium salt form or the acetoxymethyl ester (AM) form of the calcium-sensitive fluorescent probe, Indo-1. The relationship...
The cytosolic calcium transient modulates the action potential of rat ventricular myocytes.
DuBell, W H, Boyett, M R, Spurgeon, H A, Talo, A, Stern, M D, Lakatta, E G
1. The modulation of the action potential by the cytosolic Ca2+ (Cai2+) transient was studied in single isolated rat ventricular myocytes loaded with the acetoxymethyl ester form of the...
Ryanodine releases calcium from sarcoplasmic reticulum in calcium-tolerant rat cardiac myocytes.
1. The hypothesis tested in this study is that ryanodine depletes sarcoplasmic reticulum (s.r.) Ca2+ loading in suspensions of single adult rat cardiac myocytes by effecting Ca2+ release into the...
Kort, A A, Lakatta, E G, Marban, E, Stern, M D, Wier, W G
Ca2+-activated aequorin luminescence and tension were measured in dog Purkinje fibres during twitches and during the increase in resting force produced by exposure of the fibres to a low-Na+ solution...
Diastolic scattered light fluctuation, resting force and twitch force in mammalian cardiac muscle
1. When coherent light was passed through isolated isometric cardiac muscles during the diastolic or resting period, intensity fluctuations were observed in the scattered field. The frequency of...
Force staircase kinetics in mammalian cardiac muscle: modulation by muscle length
Lakatta, E. G., Spurgeon, H. A.
1. Quiescent cat papillary muscles were stimulated to contract regularly at Lmax, the length at which force production is optimal, and at 0·85 Lmax. The resulting increase in force production (rate...
Cheng, H, Song, L S, Shirokova, N, González, A, Lakatta, E G, Ríos, E, ...
Determination of the calcium spark amplitude distribution is of critical importance for understanding the nature of elementary calcium release events in striated muscle. In the present study we show,...
G(i)-dependent localization of beta(2)-adrenergic receptor signaling to L-type Ca(2+) channels.
Chen-Izu, Y, Xiao, R P, Izu, L T, Cheng, H, Kuschel, M, Spurgeon, H, ...
A plausible determinant of the specificity of receptor signaling is the cellular compartment over which the signal is broadcast. In rat heart, stimulation of beta(1)-adrenergic receptor (beta(1)-AR),...
Ion concentration-dependence of rat cardiac unitary L-type calcium channel conductance.
Guia, A, Stern, M D, Lakatta, E G, Josephson, I R
Little is known about the native properties of unitary cardiac L-type calcium currents (i(Ca)) measured with physiological calcium (Ca) ion concentration, and their role in excitation-contraction...
Increased expression of 72-kd type IV collagenase (MMP-2) in human aortic atherosclerotic lesions.
Li, Z., Li, L., Zielke, H. R., Cheng, L., Xiao, R., Crow, M. T., ...
MMP-2, a secreted 72-kd metalloproteinase that specifically degrades type IV collagen as well as denatured collagens, has been implicated in smooth muscle cell migration. To evaluate the possible...