Sreedharan, S P, Huang, J X, Cheung, M C, Goetzl, E J
Vasoactive intestinal peptide (VIP) and other members of the pituitary adenylyl cyclase-activating peptide (PACAP) and secretin neuroendocrine peptide family are recognized with specificity by...
Cloning and expression of the human vasoactive intestinal peptide receptor.
Sreedharan, S P, Robichon, A, Peterson, K E, Goetzl, E J
Vasoactive intestinal peptide (VIP) is a neuroendocrine mediator found in the central and peripheral nervous system. Distinct subsets of neural, respiratory, gastrointestinal, and immune cells bear...
The interaction of human neutrophils adherent to plastic petri dishes with the purified chemotactic factors C5a and kallikrein increased their rate of aerobic glycolysis 25-120% and the activity of...
Ruddy, S, Austen, K F, Goetzl, E J
Interaction of D (the activated form of D) and B, factors of the properdin pathway, with C3b (the major cleavage fragment of C3) generates a convertase, C3B, which cleaves C3 and initiates the...
Hyperalgesic properties of 15-lipoxygenase products of arachidonic acid.
Levine, J D, Lam, D, Taiwo, Y O, Donatoni, P, Goetzl, E J
Induction of hyperalgesia by leukotriene B4 (LTB4), a potent chemotactic factor for polymorphonuclear leukocytes (PMNLs), depends on the generation by cutaneous PMNLs of mediators that are probably...
Human T-lymphocyte subset specificity of the regulatory effects of leukotriene B4.
Payan, D G, Missirian-Bastian, A, Goetzl, E J
Leukotriene B4, but not (12S)-leukotriene B4, coupled to fluorescein-labeled human serum albumin interacts specifically with human T lymphocytes, as assessed by fluorescence-activated flow cytometry....
Identification of human eosinophil lysophospholipase as the constituent of Charcot-Leyden crystals.
Weller, P F, Goetzl, E J, Austen, K F
Since the initial descriptions of Charcot-Leyden crystals more than 100 years ago, the presence of these slender, dipyramidal crystals in human tissues and biologic fluids has become a hallmark of...
Stimulation of histamine release from human basophils by human platelet factor 4.
Brindley, L L, Sweet, J M, Goetzl, E J
Human basophils were stimulated to release histamine noncytotoxically by purified human platelet factor 4 (PF4) and the synthetic substituent peptide PF4(59-70). Histamine release was augmented...
Williams, L T, Antoniades, H N, Goetzl, E J
Platelet-derived growth factor (PDGF) stimulates both proliferation of fibroblasts and chemotaxis of leukocytes. In this study we compared the mitogenic and chemotactic activities of native PDGF and...
Mono- and dihydroxyeicosatetraenoic acids alter calcium homeostasis in rabbit neutrophils.
Naccache, P H, Sha'afi, R I, Borgeat, P, Goetzl, E J
Products of the lipoxygenation of arachidonic acid that express neutrophil chemotactic activity were examined in vitro for effects on the uptake of 45Ca by rabbit peritoneal neutrophils. At optimally...
Klickstein, L B, Shapleigh, C, Goetzl, E J
The predominant lipoxygenase products of arachidonic acid were extracted and purified from synovial fluid and sonicates of synovial tissue of patients with rheumatoid arthritis (RA),...
Hunter, J A, Finkbeiner, W E, Nadel, J A, Goetzl, E J, Holtzman, M J
Epithelial cells of 99% purity and 92% viability were isolated from human tracheas obtained post mortem, and the cellular pathways for lipoxygenation of arachidonic acid were examined in vitro. The...
Accumulation of leukotriene C4 and histamine in human allergic skin reactions.
Talbot, S F, Atkins, P C, Goetzl, E J, Zweiman, B
To determine whether lipoxygenase products of arachidonic acid metabolism are released in vivo during human allergic cutaneous reactions, we serially assayed chamber fluid placed over denuded skin...
Substance P recognition by a subset of human T lymphocytes.
Payan, D G, Brewster, D R, Missirian-Bastian, A, Goetzl, E J
The interaction of substance P with human blood T-lymphocytes, which stimulates T-lymphocyte proliferation, was quantified by both flow cytometric and direct binding assays. Fluorescence-detection...
Preferential eosinophil chemotactic activity exhibiting a molecular weight comparable to that released from sensitized human lung fragments challenged with specific antigen and designated eosinophil...
Isolation of human eosinophil phospholipase D.
Kater, L A, Goetzl, E J, Austen, K F
Phospholipase D preferentially contained in human eosinophil polymorphonuclear leukocytes as compared to other leukocytes was isolated by sequential asion and cation exchange chromatography and gel...
Baud, L, Goetzl, E J, Koo, C H
The C6-sulfidopeptide leukotrienes C4 (LTC4) and D4 (LTD4) evoked increases in the cytosolic concentration of intracellular calcium ([Ca+2]i) in dimethylsulfoxide-differentiated HL-60 cells, as...
Sreedharan, S P, Huang, J X, Cheung, M C, Goetzl, E J
Vasoactive intestinal peptide (VIP) and other members of the pituitary adenylyl cyclase-activating peptide (PACAP) and secretin neuroendocrine peptide family are recognized with specificity by...
Cloning and expression of the human vasoactive intestinal peptide receptor.
Sreedharan, S P, Robichon, A, Peterson, K E, Goetzl, E J
Vasoactive intestinal peptide (VIP) is a neuroendocrine mediator found in the central and peripheral nervous system. Distinct subsets of neural, respiratory, gastrointestinal, and immune cells bear...
The interaction of human neutrophils adherent to plastic petri dishes with the purified chemotactic factors C5a and kallikrein increased their rate of aerobic glycolysis 25-120% and the activity of...
Ruddy, S, Austen, K F, Goetzl, E J
Interaction of D (the activated form of D) and B, factors of the properdin pathway, with C3b (the major cleavage fragment of C3) generates a convertase, C3B, which cleaves C3 and initiates the...
Hyperalgesic properties of 15-lipoxygenase products of arachidonic acid.
Levine, J D, Lam, D, Taiwo, Y O, Donatoni, P, Goetzl, E J
Induction of hyperalgesia by leukotriene B4 (LTB4), a potent chemotactic factor for polymorphonuclear leukocytes (PMNLs), depends on the generation by cutaneous PMNLs of mediators that are probably...
Human T-lymphocyte subset specificity of the regulatory effects of leukotriene B4.
Payan, D G, Missirian-Bastian, A, Goetzl, E J
Leukotriene B4, but not (12S)-leukotriene B4, coupled to fluorescein-labeled human serum albumin interacts specifically with human T lymphocytes, as assessed by fluorescence-activated flow cytometry....
Identification of human eosinophil lysophospholipase as the constituent of Charcot-Leyden crystals.
Weller, P F, Goetzl, E J, Austen, K F
Since the initial descriptions of Charcot-Leyden crystals more than 100 years ago, the presence of these slender, dipyramidal crystals in human tissues and biologic fluids has become a hallmark of...
Stimulation of histamine release from human basophils by human platelet factor 4.
Brindley, L L, Sweet, J M, Goetzl, E J
Human basophils were stimulated to release histamine noncytotoxically by purified human platelet factor 4 (PF4) and the synthetic substituent peptide PF4(59-70). Histamine release was augmented...
Williams, L T, Antoniades, H N, Goetzl, E J
Platelet-derived growth factor (PDGF) stimulates both proliferation of fibroblasts and chemotaxis of leukocytes. In this study we compared the mitogenic and chemotactic activities of native PDGF and...
Mono- and dihydroxyeicosatetraenoic acids alter calcium homeostasis in rabbit neutrophils.
Naccache, P H, Sha'afi, R I, Borgeat, P, Goetzl, E J
Products of the lipoxygenation of arachidonic acid that express neutrophil chemotactic activity were examined in vitro for effects on the uptake of 45Ca by rabbit peritoneal neutrophils. At optimally...
Klickstein, L B, Shapleigh, C, Goetzl, E J
The predominant lipoxygenase products of arachidonic acid were extracted and purified from synovial fluid and sonicates of synovial tissue of patients with rheumatoid arthritis (RA),...
Hunter, J A, Finkbeiner, W E, Nadel, J A, Goetzl, E J, Holtzman, M J
Epithelial cells of 99% purity and 92% viability were isolated from human tracheas obtained post mortem, and the cellular pathways for lipoxygenation of arachidonic acid were examined in vitro. The...
Accumulation of leukotriene C4 and histamine in human allergic skin reactions.
Talbot, S F, Atkins, P C, Goetzl, E J, Zweiman, B
To determine whether lipoxygenase products of arachidonic acid metabolism are released in vivo during human allergic cutaneous reactions, we serially assayed chamber fluid placed over denuded skin...
Substance P recognition by a subset of human T lymphocytes.
Payan, D G, Brewster, D R, Missirian-Bastian, A, Goetzl, E J
The interaction of substance P with human blood T-lymphocytes, which stimulates T-lymphocyte proliferation, was quantified by both flow cytometric and direct binding assays. Fluorescence-detection...
Preferential eosinophil chemotactic activity exhibiting a molecular weight comparable to that released from sensitized human lung fragments challenged with specific antigen and designated eosinophil...
Isolation of human eosinophil phospholipase D.
Kater, L A, Goetzl, E J, Austen, K F
Phospholipase D preferentially contained in human eosinophil polymorphonuclear leukocytes as compared to other leukocytes was isolated by sequential asion and cation exchange chromatography and gel...
Baud, L, Goetzl, E J, Koo, C H
The C6-sulfidopeptide leukotrienes C4 (LTC4) and D4 (LTD4) evoked increases in the cytosolic concentration of intracellular calcium ([Ca+2]i) in dimethylsulfoxide-differentiated HL-60 cells, as...
Co-expression of EP2 and EP3 subtypes of prostaglandin E2 (PGE2) receptors (R) by U937 human monocytic cells permitted comparative studies of desensitization of each subtype. Specific binding of...
Sreedharan, S P, Goetzl, E J, Malfroy, B
The cell-surface neutral endopeptidase 3.4.24.11 (NEP) activity of the common acute lymphoblastic leukaemia antigen (CALLA) cleaves diverse peptide mediators at specific sites and it has been...
Demeter, J, Medzihradszky, D, Kha, H, Goetzl, E J, Turck, C W
Human cultured monocyte-like tumour cells of the U937 histiocyte derived line were stimulated with phorbol myristate acetate, and generated and released an 18,000 MW polypeptide fibroblast-activating...
Boggs, J M, Koo, C H, Goetzl, E J
Pretreatment of suspensions of human polymorphonuclear leucocytes (PMNL) with leukotriene B4 (LTB4) induces chemotactic deactivation, characterized by diminished expression of high-affinity LTB4...
Unique pattern of cleavage of vasoactive intestinal peptide by human lymphocytes.
Goetzl, E J, Kodama, K T, Turck, C W, Schiogolev, S A, Sreedharan, S P
Human cultured T lymphocytes of the Jurkat line and myeloma cells of the U266 line cleaved the 28 amino acid vasoactive intestinal peptide (VIP1-28) preferentially at three sites with time- and...
Turck, C W, Kennedy, P W, Schiogolev, S I, Goetzl, E J
The responses of human dermal fibroblasts to highly purified fibroblast-activating factor (FAF), derived from supernatants of U937 human monocytes stimulated with phorbol myristate acetate (PMA),...
Mouse monoclonal antibody to a latent epitope of leucocyte receptors for leukotriene B4.
Harvey, J P, Koo, C H, Boggs, J M, Young, R N, Goetzl, E J
Human blood polymorphonuclear (PMN) leucocytes and human leucocytes of the HL-60 line, which were induced to differentiate by 1,25-dihydroxyvitamin D3, express stereospecific receptors for the potent...
Induction of aggregation of Raji human B-lymphoblastic cells by vasoactive intestinal peptide.
Robichon, A, Sreedharan, S P, Yang, J, Shames, R S, Gronroos, E C, Cheng, P P, ...
Subsets of neurons in the thymic cortex, Peyer's patches and lymphoid tissues of the respiratory system deliver vasoactive intestinal peptide (VIP) at nanomolar concentrations. The possible effects...
Cheng, P P, Sreedharan, S P, Kishiyama, J L, Goetzl, E J
Vasoactive intestinal peptide (VIP1-28) is a neuromediator recognized by high-affinity receptors on human lymphocytes, which inhibits T-cell proliferation and cytokine secretion, and suppresses...
Wasserman, S. I., Goetzl, E. J., Kaliner, M., Austen, K. F.
The antigen-induced release of preformed eosinophil chemotactic factor of anaphylaxis (ECF-A) from passively sensitized human lung tissue, requires divalent cations, an intact glycolytic pathway and...
A phagocytosis-enhancing factor in human plasma.
Gigli, I, Wintroub, B U, Goetzl, E J
A phagocytosis-enhancing factor (PEF) with the capacity to stimulate the ingestion of sensitized sheep erythrocytes by human polymorphonuclear and mononuclear leucocytes has been isolated from human...
Wintroub, B U, Goetzl, E J, Austen, K F
The human neutrophil neutral peptide-generating protease was associated with the plasma membrane marker 5'-nucleotidase on sucrose density gradient centrifugation of sonicates of granule-free...
The exposure of isolated washed human neutrophils to purified human alpha1-antitrypsin resulted in a transient 2-fold enhancement of random migration and concomitant 70-90 per cent inhibition of...
Baud, L, Koo, C H, Goetzl, E J
Human polymorphonuclear (PMN) leucocytes bind synthetic [3H]-labelled leukotriene C4 ([3H]LTC4) with rapid saturation and reversibility of approximately 90%, by a 700-fold higher concentration of...
The binding of the platelet-activating factor 1-O-hexadecyl-2-acetyl-sn-glycero-3-phosphorylcholine (AGEPC) by human polymorphonuclear (PMN) leucocytes was complete within 20-30 min and optimal at 37...
Isolation of two polypeptides comprising the neutrophil-immobilizing factor of human leucocytes.
Watt, K W, Brightman, I L, Goetzl, E J
Human leucocyte lysosomal polypeptides of mol. wt 4000-5000, which constitute the neutrophil-immobolizing factor (NIF), were isolated from the 22,000 g supernate of sonicates of human neutrophils by...
Goetzl, E J, Brindley, L L, Goldman, D W
The functionally predominant constituents of the slow-reacting substance of anaphylaxis (SRS-A), designated leukotrienes C4 and D4 (LTC4 and LTD4), as well as the leucocyte chemotactic factor...
Generation of a unique fibroblast-activating factor by human monocytes.
Dohlman, J G, Payan, D G, Goetzl, E J
Purified human monocytes incubated with phytohemagglutinin (PHA), bacterial lipopolysaccharide (LPS), or serum-opsonized zymosan particles (OZ) generate human dermal fibroblast-activating activity,...
A basophil-activating factor from human T lymphocytes.
Goetzl, E J, Foster, D W, Payan, D G
Human T lymphocytes stimulated with phytohaemagglutinin (PHA) or streptokinase-streptodornase (SK-SD) generate an activity which elicits non-cytotoxic histamine release from human basophils....
Goldman, D W, Hannah, A L, Goetzl, E J
Human platelet factor 4 (PF4) and a substituent dodecapeptide designated PF4(59-70) elicited human neutrophil and monocyte chemotaxis with a similar concentration-dependence and maximal responses...
The immunological generation of a platelet-activating factor and a platet-lytic factor in the rat.
Valone, F H, Whitmer, D I, Pickett, W C, Austen, K F, Goetzl, E J
Antigen challenge of the rat peritoneal cavity which had been prepared with IgGa-rich antiserum generated activities which released [14C]-serotonin from pre-labelled human platelets. After adsorption...
The mechanism of stimulation of human PMN leucocyte-directed migration by chemotactic factors was studied by pre-labelling plasma membrane determinants with impermeant covalent reagents and assessing...
Modulation of human neutrophil function by monohydroxy-eicosatetraenoic acids.
Goetzl, E J, Brash, A R, Tauber, A I, Oates, J A, Hubbard, W C
The generation from arachidonic acid and purification of large quantities of a series of monohydroxy-eicosatetraenoic acids (HETEs) which differed only in the position of the hydroxyl group permitted...
The possibility that endogenous monohydroxy-eicosatetraenoic acids (HETEs) derived from the lipoxygenation of arachidonic acid might serve a role in human neutrophil migration was examined by...
Structural requirements for the activity of an immunologically generated lipid chemotactic factor.
The predominant lipid chemotactic factor (LCFR) generated by IgGa-dependent immunological challenge of the rat peritoneal cavity was resolved from other lipid mediators released simultaneously into...
Goetzl, E. J., Foster, D. W., Goldman, D. W.
IgG and Fab were prepared from goat antisera to MP-2, the quantitatively predominant membrane protein constituent of human neutrophil receptors for chemotactic formyl-methionyl peptides. Only...
Polymorphonuclear (PMN) leucocytes from 4 patients with untreated systemic lupus erythematosus (SLE) showed defective random migration (P less than 0-05) and depressed chemotactic responses to C5a...
Moqbel, R, Sass-Kuhn, S P, Goetzl, E J, Kay, A B
We have studied the ability of leukotrienes and other lipoxygenase products of arachidonic acid (AA) to influence complement-dependent killing of schistosomula of Schistosoma mansoni in vitro by...
Nagy, L, Lee, T H, Goetzl, E J, Pickett, W C, Kay, A B
The lipoxygenase products of arachidonic acid, 5-HPETE, 5-HETE, LTB4, LTC4 and LTD4, were examined for their capacity to enhance the expression of complement (C3b) receptors and to evoke chemotaxis...
Goetzl, E J, Rothenberg, J, Weber, E L, Sinn, C M, Austen, K F
An eosinophil chemotactic activity was identified in extracts of tumour tissue and cerebrospinal fluid from a patient with a histiocytic lymphoma of the brain and spinal cord that was infiltrated...
The phospholipid mediator 1-O-hexadecyl-2-acetyl-sn-glycero-3-phosphorylcholine (AGEPC) increases human polymorphonuclear leukocyte (PMN) adherence to Sephadex G-25 in manner directly proportional to...
Modulation of human eosinophil polymorphonuclear leukocyte migration and function.
Eosinophil migration toward a concentration gradient of a chemotactic factor is regulated at four levels. Diverse immunologic pathways generate stimuli with eosinophil chemotactic activity, including...