Proviral sequences related to the intracisternal A particle (IAP) are amplified and dispersed in the mouse genome. Their expression is associated with hypomethylation at CpG sites in the 5' long...
Multiple protein-binding sites in an intracisternal A particle long terminal repeat.
The long terminal repeats (LTRs) of cloned intracisternal A particles (IAPs) can function as effective promoters in heterologous and homologous cell types (K. K. Lueders, J. W. Fewell, E. L. Kuff,...
Mietz, J A, Grossman, Z, Lueders, K K, Kuff, E L
The 7,095-nucleotide sequence of a mouse genomic intracisternal A-particle (IAP) element, MIA14, is reported. MIA14 is known to be colinear with IAP 35S RNA and to contain functional long terminal...
Callahan, R, Kuff, E L, Lueders, K K, Birkenmeier, E
The genetic relationship between the retrovirus-like intracisternal type A particle (IAP) from Mus musculus and the novel retrovirus (M432) from M. cervicolor has been determined by heteroduplex and...
Kuff, E L, Callahan, R, Howk, R S
A specific immunological relationship has been demonstrated between the main structural protein (p73) of intracisternal A-particles and a major internal protein (p24) of the M432 retrovirus.
Sequences related to mouse intracisternal A-particle (IAP) genes have been isolated from rat and Syrian hamster gene libraries as recombinants in lambda phage. The sequences are moderately reiterated...
Tanabe, K, Karawya, E, Fewell, J W, Kuff, E L, Wilson, S H
Tightly confluent monkey cell lines BSC-1 and CV-1 held in stale medium for several days exhibited an extremely low level of thymidine incorporation into cellular DNA. Yet, these cells contained a...
The genome of M. musculus contains many copies of DNA sequences homologous to the 35S RNA of intracisternal type-A particles (IAP) (1,2). A major class of IAP genes has been identified and isolated...
The nucleotide sequence of a mouse cDNA encoding the 80 kDa subunit of the Ku (p70/p80) autoantigen.
Grossman, Z, Mietz, J A, Kuff, E L
Poly(A)RNAs prepared from the thymuses of C57BL/6J and DBA/2J mice were used to construct cDNA libraries in the bacterial expression vector lambda gt11. The libraries were scanned first for protein...
The genome of Mus musculus contains multiple copies (500 -1000) of DNA sequences related to the 35S RNA of intracisternal type A particles (IAPs). Using labeled IAP RNA as a probe in...
Genetic individuality of intracisternal A-particles of Mus musculus.
The nucleic acid sequence relationship between mouse intracisternal type A-particles and type C and B viruses was examined by reciprocal complementary DNA-RNA hybridization; complementary DNAs...
Transcription of host-substituted simian virus 40 DNA in whole cells and extracts.
Kuff, E L, Ferdinand, F J, Khoury, G
Viral transcriptional complexes were extracted from the nuclei of monkey kidney cells infected with wild-type simian virus 40 (SV40) or a variant strain containing a high proportion of...
RNA associated with murine intracisternal type A particles codes for the main particle protein.
Paterson, B M, Segal, S, Lueders, K K, Kuff, E L
Intracisternal type A particles were isolated from MOPC-104E myeloma grown subcutaneously and from N 4 neuroblastoma cells in culture. Polyadenylated RNA was prepared from the particles and tested in...
Kuff, E L, Lueders, K K, Orenstein, J M, Wilson, S H
Mouse neuroblastoma cells containing intracisternal type A particles were treated with iododeoxyuridine and dexamethasone to induce the release of type C oncornavirus particles. For 5 days after...
Intracisternal A-particle (IAP) expression in mouse cells has been correlated with hypomethylation of HhaI and HpaII sites in proviral long terminal repeats (LTRs). In a previous study, in vitro...
Mietz, J A, Fewell, J W, Kuff, E L
Intracisternal A-particle (IAP) proviral elements are abundant and widely dispersed in the mouse genome. IAP-related transcripts have been detected in normal mouse tissues where expression is under...
Selective expression of intracisternal A-particle genes in established mouse plasmacytomas.
Lueders, K K, Fewell, J W, Morozov, V E, Kuff, E L
Mouse plasmacytomas generally express higher levels of RNA transcripts from endogenous intracisternal A-particle (IAP) proviral elements than do lipopolysaccharide-stimulated normal lymphocytes....
Intracisternal A-particle (IAP)-specific sequences were 5- to 10-fold enriched in polyadenylated RNA from BALB/cJ thymus as compared with RNAs from liver, spleen, and kidney. The major transcripts of...
Lueders, K K, Fewell, J W, Kuff, E L, Koch, T
We describe experiments designed to determine whether an endogenous intracisternal A-particle (IAP) gene randomly selected from a mouse embryo library has the potential to be transcriptionally...
Intracisternal A-particle genes in Mus musculus: a conserved family of retrovirus-like elements.
Kuff, E L, Smith, L A, Lueders, K K
The structural organization of intracisternal A-particle genes has been studied, using isolates from a mouse gene library in lambda phage Charon 4A. The predominant gene form among the isolates was...
Kuff, E L, Mietz, J A, Trounstine, M L, Moore, K W, Martens, C L
Previously [Moore, K. W., Jardieu, P., Mietz, J. A., Trounstine, M. L., Kuff, E. L., Ishizaka, K. & Martens, C. L. (1986) J. Immunol. 136, 4283-4290], we examined a T-hybridoma-derived cDNA clone,...
Intracisternal A-particle genes as movable elements in the mouse genome.
Kuff, E L, Feenstra, A, Lueders, K, Smith, L, Hawley, R, Hozumi, N, ...
We analyzed two functionally defective mouse kappa light chain gene variants previously shown to contain novel insertions of repetitive DNA in their intervening sequences [Hawley, R. G., Shulman, M....
Structural gene identification and mapping by DNA-mRNA hybrid-arrested cell-free translation.
Paterson, B M, Roberts, B E, Kuff, E L
We present a simple method for directly correlating structural gene sequences in DNA with their corresponding mRNAs. This is based upon the fact that mRNA hybridized with its complementary DNA will...
Gluzman, Y., Kuff, E. L., Winocour, E.
Passage of the simian virus 40 (SV40) temperature-sensitive (ts) mutant tsD202 at the permissive temperature in each of three permissive lines of SV40-transformed monkey CV1 cells resulted in the...
Proviral sequences related to the intracisternal A particle (IAP) are amplified and dispersed in the mouse genome. Their expression is associated with hypomethylation at CpG sites in the 5' long...
Multiple protein-binding sites in an intracisternal A particle long terminal repeat.
The long terminal repeats (LTRs) of cloned intracisternal A particles (IAPs) can function as effective promoters in heterologous and homologous cell types (K. K. Lueders, J. W. Fewell, E. L. Kuff,...
Mietz, J A, Grossman, Z, Lueders, K K, Kuff, E L
The 7,095-nucleotide sequence of a mouse genomic intracisternal A-particle (IAP) element, MIA14, is reported. MIA14 is known to be colinear with IAP 35S RNA and to contain functional long terminal...
Callahan, R, Kuff, E L, Lueders, K K, Birkenmeier, E
The genetic relationship between the retrovirus-like intracisternal type A particle (IAP) from Mus musculus and the novel retrovirus (M432) from M. cervicolor has been determined by heteroduplex and...
Kuff, E L, Callahan, R, Howk, R S
A specific immunological relationship has been demonstrated between the main structural protein (p73) of intracisternal A-particles and a major internal protein (p24) of the M432 retrovirus.
Sequences related to mouse intracisternal A-particle (IAP) genes have been isolated from rat and Syrian hamster gene libraries as recombinants in lambda phage. The sequences are moderately reiterated...
Tanabe, K, Karawya, E, Fewell, J W, Kuff, E L, Wilson, S H
Tightly confluent monkey cell lines BSC-1 and CV-1 held in stale medium for several days exhibited an extremely low level of thymidine incorporation into cellular DNA. Yet, these cells contained a...
The genome of M. musculus contains many copies of DNA sequences homologous to the 35S RNA of intracisternal type-A particles (IAP) (1,2). A major class of IAP genes has been identified and isolated...
The nucleotide sequence of a mouse cDNA encoding the 80 kDa subunit of the Ku (p70/p80) autoantigen.
Grossman, Z, Mietz, J A, Kuff, E L
Poly(A)RNAs prepared from the thymuses of C57BL/6J and DBA/2J mice were used to construct cDNA libraries in the bacterial expression vector lambda gt11. The libraries were scanned first for protein...
The genome of Mus musculus contains multiple copies (500 -1000) of DNA sequences related to the 35S RNA of intracisternal type A particles (IAPs). Using labeled IAP RNA as a probe in...
Genetic individuality of intracisternal A-particles of Mus musculus.
The nucleic acid sequence relationship between mouse intracisternal type A-particles and type C and B viruses was examined by reciprocal complementary DNA-RNA hybridization; complementary DNAs...
Transcription of host-substituted simian virus 40 DNA in whole cells and extracts.
Kuff, E L, Ferdinand, F J, Khoury, G
Viral transcriptional complexes were extracted from the nuclei of monkey kidney cells infected with wild-type simian virus 40 (SV40) or a variant strain containing a high proportion of...
RNA associated with murine intracisternal type A particles codes for the main particle protein.
Paterson, B M, Segal, S, Lueders, K K, Kuff, E L
Intracisternal type A particles were isolated from MOPC-104E myeloma grown subcutaneously and from N 4 neuroblastoma cells in culture. Polyadenylated RNA was prepared from the particles and tested in...
Kuff, E L, Lueders, K K, Orenstein, J M, Wilson, S H
Mouse neuroblastoma cells containing intracisternal type A particles were treated with iododeoxyuridine and dexamethasone to induce the release of type C oncornavirus particles. For 5 days after...
Intracisternal A-particle (IAP) expression in mouse cells has been correlated with hypomethylation of HhaI and HpaII sites in proviral long terminal repeats (LTRs). In a previous study, in vitro...
Mietz, J A, Fewell, J W, Kuff, E L
Intracisternal A-particle (IAP) proviral elements are abundant and widely dispersed in the mouse genome. IAP-related transcripts have been detected in normal mouse tissues where expression is under...
Selective expression of intracisternal A-particle genes in established mouse plasmacytomas.
Lueders, K K, Fewell, J W, Morozov, V E, Kuff, E L
Mouse plasmacytomas generally express higher levels of RNA transcripts from endogenous intracisternal A-particle (IAP) proviral elements than do lipopolysaccharide-stimulated normal lymphocytes....
Intracisternal A-particle (IAP)-specific sequences were 5- to 10-fold enriched in polyadenylated RNA from BALB/cJ thymus as compared with RNAs from liver, spleen, and kidney. The major transcripts of...
Lueders, K K, Fewell, J W, Kuff, E L, Koch, T
We describe experiments designed to determine whether an endogenous intracisternal A-particle (IAP) gene randomly selected from a mouse embryo library has the potential to be transcriptionally...
Intracisternal A-particle genes in Mus musculus: a conserved family of retrovirus-like elements.
Kuff, E L, Smith, L A, Lueders, K K
The structural organization of intracisternal A-particle genes has been studied, using isolates from a mouse gene library in lambda phage Charon 4A. The predominant gene form among the isolates was...
Kuff, E L, Mietz, J A, Trounstine, M L, Moore, K W, Martens, C L
Previously [Moore, K. W., Jardieu, P., Mietz, J. A., Trounstine, M. L., Kuff, E. L., Ishizaka, K. & Martens, C. L. (1986) J. Immunol. 136, 4283-4290], we examined a T-hybridoma-derived cDNA clone,...
Intracisternal A-particle genes as movable elements in the mouse genome.
Kuff, E L, Feenstra, A, Lueders, K, Smith, L, Hawley, R, Hozumi, N, ...
We analyzed two functionally defective mouse kappa light chain gene variants previously shown to contain novel insertions of repetitive DNA in their intervening sequences [Hawley, R. G., Shulman, M....
Structural gene identification and mapping by DNA-mRNA hybrid-arrested cell-free translation.
Paterson, B M, Roberts, B E, Kuff, E L
We present a simple method for directly correlating structural gene sequences in DNA with their corresponding mRNAs. This is based upon the fact that mRNA hybridized with its complementary DNA will...
Gluzman, Y., Kuff, E. L., Winocour, E.
Passage of the simian virus 40 (SV40) temperature-sensitive (ts) mutant tsD202 at the permissive temperature in each of three permissive lines of SV40-transformed monkey CV1 cells resulted in the...
Intracisternal Type A particles (IAPs) are retroviruslike structures identified by a core protein antigen (p73) and found in mouse embryos, in many mouse tumor cells, and in pancreatic B cells of...
Kuff, E. L., Hymer, W. C., Shelton, E., Roberts, N. E.
Cytoplasmic extracts of the transplantable RPC-20 plasma-cell tumor were fractionated by sucrose density gradient centrifugation. Four major fractions were distinguished: (a) microsomes and...