Johnson, E. M., Diena, B. B., Lior, H., Ryan, A., Krol, P.
Pub. in Infection and Immunity, v38 n1 p201-205, Oct 1982.
CAPILLARY DEVELOPMENT DURING EXPOSURE TO CHRONIC HYPOXIA. (2005)
Cassin,S., Gilbert,R. D., Johnson,E. M.
A group of 18 male rats weighing initially 110 to 131 gm. was exposed to a pressure altitude of 20,000 feet for 36 days in a decompression chamber. A similar group of 18 male rats was maintained at...
Perspectives on the Utility of Systems Science in the Army. (2002)
This report presents the results of a conference designed to develop consensus on a workable definition of systems science, determine its potential for solution of Army problems, and develop...
Johnson, E. M, Ewing, S. A, Barker, R. W, Fox, J. C, Crow, D. W, Kocan, K. M
Four trials were conducted in which laboratory-reared Dermacentor variabilis nymphs were exposed to Ehrlichia canis by feeding on experimentally infected dogs as soon as classical morulae were...
Vectors of canine ehrlichiosis: impact on zoonosis (1989)
Ewing, S. A, Barker, R. W, Johnson, E. M, Harkess, J. R, Kocan, K. M, Fox, J. C, ...
Chen, N N, Chang, C F, Gallia, G L, Kerr, D A, Johnson, E M, Krachmarov, C P, ...
Human JC polyomavirus (JCV) is the etiologic agent of the neurodegenerative disease progressive mulifocal leukoencephalopathy. By using JCV as a model, we investigated the role of the viral early...
Radford, S A, Johnson, E M, Warnock, D W
The in vitro activity of voriconazole was compared with that of itraconazole. Eighty-six isolates of pathogenic molds belonging to 23 species were tested by an agar dilution method in High Resolution...
Influence of food on the pharmacokinetics of ketoconazole.
Daneshmend, T K, Warnock, D W, Ene, M D, Johnson, E M, Potten, M R, Richardson, M D, ...
Eight healthy adults were given single oral doses of ketoconazole (200, 400, 600, and 800 mg) in the fasting state and with a standard breakfast at weekly intervals according to a balanced block...
Fluconazole resistance in Candida glabrata.
Hitchcock, C A, Pye, G W, Troke, P F, Johnson, E M, Warnock, D W
We report a case of infection with Candida glabrata in which the organism became resistant to fluconazole and in which pre- and posttreatment isolates were available for comparison. The organism was...
Nichol, P F, Chang, J Y, Johnson, E M, Olivo, P D
Herpes simplex virus establishes a latent infection in peripheral neurons. We examined viral gene expression in rat peripheral neurons in vitro and determined that viral gene expression is attenuated...
Genetic regulation of variable Vi antigen expression in a strain of Citrobacter freundii.
Snellings, N J, Johnson, E M, Kopecko, D J, Collins, H H, Baron, L S
Certain strains of the genus Citrobacter exhibit a variable expression of the Vi surface antigen that appears to involve a special mechanism for regulation of gene expression. Two nonlinked...
Plasmid-Determined Ability of a Salmonella tennessee Strain to Ferment Lactose and Sucrose
Johnson, E. M., Wohlhieter, J. A., Placek, Bruce P., Sleet, R. B., Baron, L. S.
The ability of a Salmonella tennessee strain to ferment both lactose and sucrose was attributed to a conjugally transmissible plasmid, deoxyribonucleic acid molecular weight 164 × 106, bearing the...
Genetic Basis of Vi Antigen Expression in Salmonella paratyphi C
Snellings, Norma J., Johnson, E. M., Baron, L. S.
Analysis of hybrids formed in a cross between a Salmonella paratyphi C Hfr and an S. typhimurium recipient indicated that the structural genetic determinants of the S. paratyphi C Vi antigen are...
Johnson, E M, Placek, B P, Snellings, N J, Baron, L S
A partially diploid Escherichia coli hybrid recovered from mating with a Salmonella typhimurium donor was converted to an Hfr strain, designated WR2080, as a means to examine the manner in which the...
Characterization of transmissible genetic elements from sucrose-fermenting Salmonella strains.
Wohlhieter, J A, Lazere, J R, Snellings, N J, Johnson, E M, Synenki, R M, Baron, L S
Two of seven sucrose-fermenting Salmonella strains obtained from clinical sources were found capable of conjugal transfer of the sucrose fermentation (Scr+) property to the Escherichia coli K-12...
Johnson, E. M., Craig, William G., Wohlhieter, J. A., Lazere, Janet R., Synenki, R. M., Baron, L. S.
Partially diploid Escherichia coli K-12 hybrids recovered from mating with a Salmonella typhimurium Hfr strain were found to differ with respect to the manner in which they conserved the added...
Synenki, R. M., Wohlhieter, J. A., Johnson, E. M., Lazere, Janet R., Baron, L. S.
Six lac elements originally contained in Salmonella strains were transferred to Escherichia coli WR3026. All of the six E. coli strains that received one of the lac elements were observed to contain...
Leavitt, R. W., Wohlhieter, J. A., Johnson, E. M., Olson, G. E., Baron, L. S.
Heterozygous, partial diploid Salmonella typhosa hybrids obtained from matings with Escherichia coli K-12 Hfr strains were observed to contain supercoiled, circular deoxyribonucleic acid (DNA) when...
Recombination of Escherichia coli Chromosomal Segments in Salmonella typhosa
Johnson, E. M., Alexeichik, J. A., Baron, L. S.
Approximately half of Salmonella typhosa hybrids resulting from mating with Escherichia coli Hfr donors inherit the selected donor marker by recombination, and the length of the E. coli chromosomal...
Lytic Replication of Coliphage Lambda in Salmonella typhosa Hybrids
Baron, L. S., Ryman, I. R., Johnson, E. M., Gemski, P.
Hybrids between Escherichia coli K-12 and Salmonella typhosa which conserved a continuous K-12 chromosomal diploid segment extending from pro through ara to the strA locus were sensitive to plaque...
Inefficiency of Genetic Recombination in Hybrids Between Escherichia coli and Salmonella typhosa
Johnson, E. M., Easterling, S. B., Baron, L. S.
An Escherichia coli Hfr strain in which three negative chromosomal alleles (leu−, arg−, and mtl−) were closely linked to three positive alleles (ara+, rha+, and xyl+, respectively) was employed...
Genetic Transfer of the Vi Antigen from Salmonella typhosa to Escherichia coli
The Vi antigen was expressed in a strain of Escherichia coli after transfer of the viaB locus from a Salmonella typhosa Hfr donor.
Characterization of nerve growth factor-dependent herpes simplex virus latency in neurons in vitro.
Primary sympathetic neuronal cultures were maintained for up to 5 weeks after inoculation with herpes simplex virus (HSV) without evidence of viral infection. Treatment with acyclovir for the first 7...
Genetic Analysis of the ViA-his Chromosomal Region in Salmonella
Johnson, E. M., Krauskopf, Barbara, Baron, L. S.
Johnson, E. M. (Walter Reed Army Institute of Research, Washington, D.C.), Barbara Krauskopf, and L. S. Baron. Genetic analysis of the ViA-his chromosomal region in Salmonella. J. Bacteriol....
Somatic Antigen 2 Inheritance in Salmonella Groups B and D
Somatic (O) antigen 2 of Salmonella paratyphi A replaced somatic antigen 4 of an S. typhimurium recipient as the consequence of mating with an S. paratyphi A var. durazzo Hfr strain. The genetic...
RECIPIENT ABILITY OF SALMONELLA TYPHOSA IN GENETIC CROSSES WITH ESCHERICHIA COLI1
Johnson, E. M., Falkow, Stanley, Baron, L. S.
Johnson, E. M. (Walter Reed Army Institute of Research, Washington, D.C.), Stanley Falkow, and L. S. Baron. Recipient ability of Salmonella typhosa in genetic crosses with Escherichia coli. J....
CHROMOSOME TRANSFER KINETICS OF SALMONELLAHfr STRAINS
Johnson, E. M., Falkow, Stanley, Baron, L. S.
Johnson, E. M. (Walter Reed Army Institute of Research, Washington, D.C.), Stanley Falkow, and L. S. Baron. Chromosome transfer kinetics of Salmonella Hfr strains. J. Bacteriol. 88:395–400....
Identification of a truncated form of the nerve growth factor receptor.
Schwann cells express growth factor (NGF) receptors on their cell surface in response to axotomy, a phenomenon that can be demonstrated both in vivo and in vitro. The predominant form of the NGF...
Asbestos fibers mediate transformation of monkey cells by exogenous plasmid DNA.
Appel, J D, Fasy, T M, Kohtz, D S, Kohtz, J D, Johnson, E M
We have tested the ability of chrysotile asbestos fibers to introduce plasmid DNA into monkey COS-7 cells and the ability of this DNA to function in both replication and gene expression. Chrysotile...
Nerve growth factor deprivation results in the reactivation of latent herpes simplex virus in vitro.
Primary sympathetic neuronal cultures were maintained for up to 5 weeks after inoculation with herpes simplex virus (HSV) without evidence of viral infection. Upon deprivation of nerve growth factor,...
Johnson, E. M., Easterling, S. B., Baron, L. S.
Heterozygous, partial diploid hybrids were obtained in a Salmonella typhosa Hfr strain by using it as the recipient in a mating with the Escherichia coli Hfr donor WR2004 (O...proA...leu). Three of...
Koike, T, Martin, D P, Johnson, E M
Sympathetic neurons depend on nerve growth factor (NGF) for their survival both in vivo and in vitro; these cells die upon acute deprivation of NGF. We studied the effects of agents that cause...
Nature of Lactose-fermenting Salmonella Strains Obtained from Clinical Sources
Easterling, S. B., Johnson, E. M., Wohlhieter, J. A., Baron, L. S.
Six of seven lactose-fermenting (lac+) Salmonella strains obtained from clinical sources were found to be capable of transferring the lac+ property by conjugation to Salmonella typhosa WR4204. All of...
Genetic Mapping of Vi and Somatic Antigenic Determinants in Salmonella
Johnson, E. M., Krauskopf, Barbara, Baron, L. S.
Johnson, E. M. (Walter Reed Army Institute of Research, Washington, D.C.), Barbara Krauskopf, and L. S. Baron. Genetic mapping of Vi and somatic antigenic determinants in Salmonella. J. Bacteriol....
Nerve growth factor receptor molecules in rat brain.
Taniuchi, M, Schweitzer, J B, Johnson, E M
We have developed a method to immunoprecipitate rat nerve growth factor (NGF) receptor proteins and have applied the method to detect NGF receptor molecules in the rat brain. Crosslinking...
Induction of nerve growth factor receptor in Schwann cells after axotomy.
Taniuchi, M, Clark, H B, Johnson, E M
We have discovered that axotomy of sciatic nerve induces Schwann cells distal to the lesion to express de novo, or at greatly increased levels, receptors for nerve growth factor (NGF). Surgical...
Replication of a plasmid bearing a human Alu-family repeat in monkey COS-7 cells.
Monkey COS-7 cells were transformed with BLUR8 DNA, a pBR322 plasmid containing a human Alu-family sequence at the BamHI site. Within 24 hr of transformation 2-5% of the BLUR8 molecules recovered...
Campbell, G R, Littau, V C, Melera, P W, Allfrey, V G, Johnson, E M
R-loop and restriction mapping procedures reveal the organization of coding regions at each end of the giant rDNA palindrome of Physarum polycephalum. A 19S coding region of 2.10 +/- 0.21 kb is...
We describe a sensitive method for mapping replication initiation sites near regions of sequenced genomic DNA in vivo. It is based on selective amplification of sets of segments in purified nascent...
A cell-free system using synchronous plasmodial extracts initiates replication selectively on the 60 kb rDNA palindrome of Physarum polycephalum. Preferential labeling of rDNA fragments by nuclear...
The subunit structure of chromatin from Physarum polycephalum.
Johnson, E M, Littau, V C, Allfrey, V G, Bradbury, E M, Matthews, H R
Nucleosome DNA repeat lengths in Physarum chromatin, determined by nuclease digestion experiments, are shorter than those observed in most mammalian chromatin and longer than those reported for...
Johnson, E. M., Diena, B. B., Lior, H., Ryan, A., Krol, P., Baron, L. S.
A mouse-virulent Salmonella typhimurium hybrid (H42), which expresses the Salmonella typhi Vi antigen in addition to S. typhi O antigens 9 and 12, and a mouse-virulent S. typhimurium hybrid (H1),...
Bergemann, A D, Ma, Z W, Johnson, E M
The human Pur factor binds strongly to a sequence element repeated within zones of initiation of DNA replication in several eukaryotic cells. The protein binds preferentially to the purine-rich...
Studies on origins of DNA replication in mammalian cells have long been hampered by a lack of methods sensitive enough for the localization of such origins in chromosomal DNA. We have employed a new...
A major site of DNA bending is located 1.6 kb upstream of the P1 transcription start site of the human c-myc gene, near the center of a reported zone of initiation of DNA replication. A repeated,...
Injury to the peripheral processes produces a profound cell loss (40-50%) in the dorsal root ganglion of newborn rats. Although division of central processes produces little or no cellular change in...
Johnson, E M, Allfrey, V G, Bradbury, E M, Matthews, H R
The localization of DNA sequences coding for ribosomal RNA was studied by hybridization of purified ribosomal RNA to DNA from chromatin fragments prepared by limited digestion of Physarum nuclei with...
Mouse protective capabilities of Escherichia coli hybrids expressing Salmonella typhi antigens.
Diena, B B, Lior, H, Ryan, A, Krol, P, Johnson, E M, Baron, L S
An Escherichia coli hybrid, F1061, expressing Salmonella typhi somatic antigens 9 and 12, and a derivative of this hybrid, E. coli hybrid WR3078, expressing the S. typhi Vi antigen in addition to...
Role of Typhoid Antigens in Protection and Pathogenicity for Mice
Diena, B. B., Baron, L. S., Johnson, E. M., Wallace, R., Ashton, F. E.
Swiss white mice immunized with acetone-killed vaccines prepared from strains of Salmonella typhosa, S. typhimurium, and mouse-virulent S. typhimurium hybrids which had acquired, by conjugal genetic...
Diena, B B, Ryan, A, Wallace, R, Ashton, F E, Johnson, E M, Baron, L S
Purified Vi antigen, acetic anthydride-treated Salmonella typhosa endotoxin, and potassium methylate-treated S. typhosa endotoxin employed as vaccines in Swiss white mice failed to protect these...
Diena, B B, Ryan, A, Wallace, R, Johnson, E M, Baron, L S, Ashton, F E
Live Salmonella typhi administered intraperitoneally, acetone-killed S. typhi administered intraperitoneally, and live S. typhi given orally, with their effectiveness decreasing in that order,...
Assay of Typhoid Vaccines with Salmonella typhosa-Salmonella typhimurium Hybrids
Diena, B. B., Johnson, E. M., Baron, L. S., Wallace, R., Greenberg, L.
Salmonella typhimurium hybrids expressing the S. typhosa antigens 9, d, and Vi were constructed by genetic crosses with an S. typhosa Hfr donor. The hybrids retained the same degree of mouse...
Intraperitoneal Mouse Virulence of Salmonella typhimurium Hybrids Expressing Somatic Antigen 9
Johnson, E. M., Snellings, Norma J., Life, Charles A., Baron, L. S.
Salmonella typhimurium hybrids expressing somatic antigen 9 after mating with either S. typhosa or S. enteritidis Hfr donors did not differ from their S. typhimurium parent with respect to the number...
Hopwood, V, Johnson, E M, Cornish, J M, Foot, A B, Evans, E G, Warnock, D W
AIMS--To evaluate the Pastorex aspergillus antigen latex agglutination test for the diagnosis of invasive aspergillosis in patients undergoing liver or bone marrow transplantation. METHODS--Serum...
Cheng, Y, Deshmukh, M, D'Costa, A, Demaro, J A, Gidday, J M, Shah, A, ...
Programmed cell death (apoptosis) is a normal process in the developing nervous system. Recent data suggest that certain features seen in the process of programmed cell death may be favored in the...
Hindered diffusion in agarose gels: test of effective medium model.
Johnson, E M, Berk, D A, Jain, R K, Deen, W M
The diffusivities of uncharged macromolecules in gels (D) are typically lower than in free solution (D infinity), because of a combination of hydrodynamic and steric factors. To examine these...
Diffusion and partitioning of proteins in charged agarose gels.
Johnson, E M, Berk, D A, Jain, R K, Deen, W M
The effects of electrostatic interactions on the diffusion and equilibrium partitioning of fluorescein-labeled proteins in charged gels were examined using fluorescence recovery after photobleaching...
Pfaller, M. A., Diekema, D. J., Rex, J. H., Espinel-Ingroff, A., Johnson, E. M., Andes, D., ...
Developing interpretive breakpoints for any given organism-drug combination requires integration of the MIC distribution, pharmacokinetic and pharmacodynamic parameters, and the relationship between...
Substance abuse and women's health.
The prevalence of illicit drug use is higher among men than women, but new drug use occurs at twice the rate for females as for males. Recent data from emergency rooms and medical examiners support...
Theories and models supporting prevention approaches to alcohol problems among youth.
Johnson, E M, Amatetti, S, Funkhouser, J E, Johnson, S
The Alcohol, Drug Abuse, and Mental Health Administration's Office for Substance Abuse Prevention (OSAP) was established to initiate programs to provide prevention and early intervention services for...
Developmental toxicity and structure/activity correlates of glycols and glycol ethers.
Johnson, E M, Gabel, B E, Larson, J
In recent years, the National Toxicology Program (NTP) has selected numerous glycol ethers for testing in routine laboratory mammals to ascertain the magnitude of their ability to injure the...
Reaching Hispanics with messages to prevent alcohol and other drug abuse.
Hispanics are often labeled a "hard-to-reach" population in the context of health communications, yet there are indicators that corporate interests increasingly are reporting successes in designing...
Chen, N N, Chang, C F, Gallia, G L, Kerr, D A, Johnson, E M, Krachmarov, C P, ...
Human JC polyomavirus (JCV) is the etiologic agent of the neurodegenerative disease progressive mulifocal leukoencephalopathy. By using JCV as a model, we investigated the role of the viral early...
Radford, S A, Johnson, E M, Warnock, D W
The in vitro activity of voriconazole was compared with that of itraconazole. Eighty-six isolates of pathogenic molds belonging to 23 species were tested by an agar dilution method in High Resolution...
Influence of food on the pharmacokinetics of ketoconazole.
Daneshmend, T K, Warnock, D W, Ene, M D, Johnson, E M, Potten, M R, Richardson, M D, ...
Eight healthy adults were given single oral doses of ketoconazole (200, 400, 600, and 800 mg) in the fasting state and with a standard breakfast at weekly intervals according to a balanced block...
Fluconazole resistance in Candida glabrata.
Hitchcock, C A, Pye, G W, Troke, P F, Johnson, E M, Warnock, D W
We report a case of infection with Candida glabrata in which the organism became resistant to fluconazole and in which pre- and posttreatment isolates were available for comparison. The organism was...
Nichol, P F, Chang, J Y, Johnson, E M, Olivo, P D
Herpes simplex virus establishes a latent infection in peripheral neurons. We examined viral gene expression in rat peripheral neurons in vitro and determined that viral gene expression is attenuated...
Genetic regulation of variable Vi antigen expression in a strain of Citrobacter freundii.
Snellings, N J, Johnson, E M, Kopecko, D J, Collins, H H, Baron, L S
Certain strains of the genus Citrobacter exhibit a variable expression of the Vi surface antigen that appears to involve a special mechanism for regulation of gene expression. Two nonlinked...
Plasmid-Determined Ability of a Salmonella tennessee Strain to Ferment Lactose and Sucrose
Johnson, E. M., Wohlhieter, J. A., Placek, Bruce P., Sleet, R. B., Baron, L. S.
The ability of a Salmonella tennessee strain to ferment both lactose and sucrose was attributed to a conjugally transmissible plasmid, deoxyribonucleic acid molecular weight 164 × 106, bearing the...
Genetic Basis of Vi Antigen Expression in Salmonella paratyphi C
Snellings, Norma J., Johnson, E. M., Baron, L. S.
Analysis of hybrids formed in a cross between a Salmonella paratyphi C Hfr and an S. typhimurium recipient indicated that the structural genetic determinants of the S. paratyphi C Vi antigen are...