D-serine deaminase is a stringent selective marker in genetic crosses.
The presence of the locus for D-serine deaminase (dsd) renders bacteria resistant to growth inhibition by D-serine and enables them to grow with D-serine as the sole nitrogen source. The two...
Organization and transcriptional regulation of the Escherichia coli K-12 D-serine tolerance locus.
Nørregaard-Madsen, M, McFall, E, Valentin-Hansen, P
We have reinvestigated the genetic organization and the transcription regulation of the dsd operon of Escherichia coli. By combining genetic and biochemical studies, it is demonstrated that the...
Effects of structural changes in the dsdA-dsdC intergenic region on D-serine deaminase synthesis.
McFall, E, Nikam, S S, Palchaudhuri, S
Single-base-pair changes well upstream of its transcription initiation site resulted in partially to fully constitutive expression of the D-serine deaminase structural gene, dsdA, independently of...
DNA sequence of the D-serine deaminase activator gene dsdC.
Palchaudhuri, S, Patel, V, McFall, E
We have determined the DNA sequence of dsdC, the gene that encodes the D-serine deaminase activator protein of Escherichia coli K-12. The sequence contains a single open reading frame that terminates...
Bornstein-Forst, S M, McFall, E, Palchaudhuri, S
The D-serine deaminase structural (dsdA) and regulatory (dsdC) genes are transcribed with opposite polarity from an intergenic region comprising more than 600 base pairs. The order of genes in the...
Purification and characterization of D-serine deaminase activator protein.
Heincz, M C, Bornstein, S M, McFall, E
We purified the dsdC gene product, the specific activator of dsdA (D-serine deaminase) gene expression, to about 25% homogeneity from a strain in which its expression was amplified 100-fold. The...
We determined the DNA sequence of the D-serine deaminase promoter region and of the N-terminal region of the structural gene. There are possibilities in the promoter for secondary structure and for...
Role of the dsdC activator in regulation of D-serine deaminase synthesis.
The activator of the D-serine deaminase operon, the product of the dsdC gene, has been partially purified. It is reasonably stable to routine purification procedures in the presence of its ligand...
Role of small molecules in regulation of D-serine deaminase synthesis.
Cyclic AMP is required for optimal synthesis of D-serine deaminase synthesis from dsdO+ templates and for optimal hyperinducible synthesis from low constitutive dsdO templates both in vitro and in...
Identification and control of synthesis of the dsdC activator protein.
Operon fusions between the D-serine deaminase regulatory and structural genes and lacZ were constructed and used to examine the control of expression of the positive regulatory gene, dsdC....
Palchaudhuri, S, McFall, E, Carothers, A M
We have obtained two classes of double lysogens that on induction yield higher titers of lambda-d dsd transducing phage than of helper phage. One class was obtained by lysogenization of strain EM6116...
The primary D-serine deaminase (D-serine dehydratase, EC 4.2.1.14) of Escherichia coli K-12 is unstable within the cell. The protein, a single polypeptide chain, is cleaved at a lysine residue by a...
The N-terminal amino acid sequences of the D-serine deaminases from strains of Escherichia coli K-12 that harbor wild-type and high-level constitutive catabolite-insensitive operator-initiator...
Escherichia coli K-12 mutant forming a temperature-sensitive D-serine deaminase.
A single-site mutant of Escherichia coli K-12 able to grow in minimal medium in the presence of D-serine at 30 C but not at 42 C was isolated. The mutant forms a D-serine deaminase that is much more...
Mutants constitutive for D-serine deaminase (Dsdase) synthesis were isolated by utilizing D-serine as sole nitrogen or carbon source in the chemostat. This method generated only regulatory...
Positive control in the D-serine deaminase system of Escherichia coli K-12.
Bloom, F R, McFall, E, Young, M C, Carothers, A M
Two new types of D-serine deaminase (Dsdase)-negative mutants have been isolated and characterized. The first fails to synthesize a functional dsdC gene product as a result of dsdC- (regulator...
Carothers, A M, McFall, E, Palchaudhuri, S
The genes dsdA, dsdO, and dsdC have been located on a 3.0-kilobase pair (kb) fragment of the Escherichia coli chromosome by a combination of techniques. The loci were first cloned onto lambda and...
Position effect on expression of dsd genes cloned onto multicopy plasmids.
Carothers, A M, Heincz, M C, McFall, E
In the D-serine deaminase system of Escherichia coli, which is regulated by positive control, we have fouand a complete lack of trans activation in vivo with multicopy dsd hybrid plasmids. A PLASmid...
Positive control of D-serine deaminase synthesis in vitro.
Heincz, M C, Kelker, N E, McFall, E
Efficient constitutive synthesis of D-serine deaminase [D-serine hydro-lyase (deaminating); EC 4.2.1.14] is obtained in vitro by using a slightly modified Zubay system programmed with dsdO6 dsdA+DNA....
D-serine deaminase is a stringent selective marker in genetic crosses.
The presence of the locus for D-serine deaminase (dsd) renders bacteria resistant to growth inhibition by D-serine and enables them to grow with D-serine as the sole nitrogen source. The two...
Organization and transcriptional regulation of the Escherichia coli K-12 D-serine tolerance locus.
Nørregaard-Madsen, M, McFall, E, Valentin-Hansen, P
We have reinvestigated the genetic organization and the transcription regulation of the dsd operon of Escherichia coli. By combining genetic and biochemical studies, it is demonstrated that the...
Effects of structural changes in the dsdA-dsdC intergenic region on D-serine deaminase synthesis.
McFall, E, Nikam, S S, Palchaudhuri, S
Single-base-pair changes well upstream of its transcription initiation site resulted in partially to fully constitutive expression of the D-serine deaminase structural gene, dsdA, independently of...
DNA sequence of the D-serine deaminase activator gene dsdC.
Palchaudhuri, S, Patel, V, McFall, E
We have determined the DNA sequence of dsdC, the gene that encodes the D-serine deaminase activator protein of Escherichia coli K-12. The sequence contains a single open reading frame that terminates...
Bornstein-Forst, S M, McFall, E, Palchaudhuri, S
The D-serine deaminase structural (dsdA) and regulatory (dsdC) genes are transcribed with opposite polarity from an intergenic region comprising more than 600 base pairs. The order of genes in the...
Purification and characterization of D-serine deaminase activator protein.
Heincz, M C, Bornstein, S M, McFall, E
We purified the dsdC gene product, the specific activator of dsdA (D-serine deaminase) gene expression, to about 25% homogeneity from a strain in which its expression was amplified 100-fold. The...
We determined the DNA sequence of the D-serine deaminase promoter region and of the N-terminal region of the structural gene. There are possibilities in the promoter for secondary structure and for...
Role of the dsdC activator in regulation of D-serine deaminase synthesis.
The activator of the D-serine deaminase operon, the product of the dsdC gene, has been partially purified. It is reasonably stable to routine purification procedures in the presence of its ligand...
Role of small molecules in regulation of D-serine deaminase synthesis.
Cyclic AMP is required for optimal synthesis of D-serine deaminase synthesis from dsdO+ templates and for optimal hyperinducible synthesis from low constitutive dsdO templates both in vitro and in...
Identification and control of synthesis of the dsdC activator protein.
Operon fusions between the D-serine deaminase regulatory and structural genes and lacZ were constructed and used to examine the control of expression of the positive regulatory gene, dsdC....
Palchaudhuri, S, McFall, E, Carothers, A M
We have obtained two classes of double lysogens that on induction yield higher titers of lambda-d dsd transducing phage than of helper phage. One class was obtained by lysogenization of strain EM6116...
The primary D-serine deaminase (D-serine dehydratase, EC 4.2.1.14) of Escherichia coli K-12 is unstable within the cell. The protein, a single polypeptide chain, is cleaved at a lysine residue by a...
The N-terminal amino acid sequences of the D-serine deaminases from strains of Escherichia coli K-12 that harbor wild-type and high-level constitutive catabolite-insensitive operator-initiator...
Escherichia coli K-12 mutant forming a temperature-sensitive D-serine deaminase.
A single-site mutant of Escherichia coli K-12 able to grow in minimal medium in the presence of D-serine at 30 C but not at 42 C was isolated. The mutant forms a D-serine deaminase that is much more...
Mutants constitutive for D-serine deaminase (Dsdase) synthesis were isolated by utilizing D-serine as sole nitrogen or carbon source in the chemostat. This method generated only regulatory...
Positive control in the D-serine deaminase system of Escherichia coli K-12.
Bloom, F R, McFall, E, Young, M C, Carothers, A M
Two new types of D-serine deaminase (Dsdase)-negative mutants have been isolated and characterized. The first fails to synthesize a functional dsdC gene product as a result of dsdC- (regulator...
Carothers, A M, McFall, E, Palchaudhuri, S
The genes dsdA, dsdO, and dsdC have been located on a 3.0-kilobase pair (kb) fragment of the Escherichia coli chromosome by a combination of techniques. The loci were first cloned onto lambda and...
Position effect on expression of dsd genes cloned onto multicopy plasmids.
Carothers, A M, Heincz, M C, McFall, E
In the D-serine deaminase system of Escherichia coli, which is regulated by positive control, we have fouand a complete lack of trans activation in vivo with multicopy dsd hybrid plasmids. A PLASmid...
Positive control of D-serine deaminase synthesis in vitro.
Heincz, M C, Kelker, N E, McFall, E
Efficient constitutive synthesis of D-serine deaminase [D-serine hydro-lyase (deaminating); EC 4.2.1.14] is obtained in vitro by using a slightly modified Zubay system programmed with dsdO6 dsdA+DNA....