Mackow, E R, Yamanaka, M Y, Dang, M N, Greenberg, H B
DNA amplification-restricted transcription-translation (DARTT), is based on DNA amplification by the polymerase chain reaction (PCR) and uses PCR to truncate protein-encoding DNA while adding...
Rotavirus diarrhea is caused by nonreplicating viral particles.
Shaw, R D, Hempson, S J, Mackow, E R
Rotaviruses infect the villous epithelium of the small intestine and cause severe diarrhea in young children. The mechanism by which rotavirus causes diarrhea has not been elucidated. It has been...
Hjelle, B, Lee, S W, Song, W, Torrez-Martinez, N, Song, J W, Yanagihara, R, ...
The complete M segment sequences of hantaviruses amplified from tissues of a patient with hantavirus pulmonary syndrome in the northeastern United States and from white-footed mice, Peromyscus...
Identification and baculovirus expression of the VP4 protein of the human group B rotavirus ADRV.
Mackow, E R, Werner-Eckert, R, Fay, M E, Tao, H, Chen, G
A complete cDNA copy of the fourth RNA segment of the human group B rotavirus adult diarrheal rotavirus (ADRV) has been cloned into lambda phage and excised into plasmid pSK Bluescript. Gene segment...
VP4-specific intestinal antibody response to rotavirus in a murine model of heterotypic infection.
Shaw, R D, Groene, W S, Mackow, E R, Merchant, A A, Cheng, E H
We have adapted a murine model of heterotypic rotavirus infection for the purpose of evaluating the intestinal antibody response to an infection that mimics human vaccination. Neonatal mice were...
Mackow, E R, Barnett, J W, Chan, H, Greenberg, H B
Rhesus rotavirus (RRV) gene 4 was cloned into lambda bacteriophage, inserted into a polyhedrin promoter shuttle plasmid, and expressed in Sf9 cells by a recombinant baculovirus. The...
Sequence analysis of gene 11 equivalents from "short" and "super short" strains of rotavirus.
Matsui, S M, Mackow, E R, Matsuno, S, Paul, P S, Greenberg, H B
The molecular basis for the aberrant migration pattern of the gene 11 equivalent in rotaviruses with "short" (human DS-1) and "super short" (human 69M and bovine VMRI) electropherotypes was...
NS35 and not vp7 is the soluble rotavirus protein which binds to target cells.
Bass, D M, Mackow, E R, Greenberg, H B
Recent studies using radiolabeled rotavirus lysates have demonstrated a 35-kilodalton viral protein that binds specifically to the surface of MA104 cells (N. Fukuhara, O. Yoshie, S. Kitakoa, and T....
Mackow, E R, Vo, P T, Broome, R, Bass, D, Greenberg, H B
A baculovirus-expressed VP4 protein derived from the simian rhesus rotavirus (RRV) was used to parenterally immunize murine dams. VP4-immunized dams developed high levels of neutralizing antibodies...
Dharakul, T, Labbe, M, Cohen, J, Bellamy, A R, Street, J E, Mackow, E R, ...
Clearance of chronic murine rotavirus infection in SCID mice can be demonstrated by adoptive transfer of immune CD8+ T lymphocytes from histocompatible donor mice immunized with a murine homotypic...
Serotypic analysis of VP3 and VP7 neutralization escape mutants of rhesus rotavirus.
Shaw, R D, Mackow, E R, Dyall-Smith, M L, Lazdins, I, Holmes, I H, Greenberg, H B
Neutralization escape mutants of simian rotaviruses (rhesus rotavirus and SA11) were tested in hemagglutination inhibition and neutralization assays against hyperimmune and infection sera to...
Serologic analysis of human rotavirus serotypes P1A and P2 by using monoclonal antibodies.
Padilla-Noriega, L, Werner-Eckert, R, Mackow, E R, Gorziglia, M, Larralde, G, Taniguchi, K, ...
Three human rotavirus (HRV) VP4 serotypes and one subtype have been described on the basis of a fourfold or an eightfold-or-greater difference in neutralization titer when tested with hyperimmune...
Padilla-Noriega, L, Fiore, L, Rennels, M B, Losonsky, G A, Mackow, E R, Greenberg, H B
The humoral immune response to rhesus rotavirus (RRV) VP4 and its cleavage products VP5* and VP8* was determined in paired serum samples from 44 infants vaccinated with RRV or human rotavirus-RRV...
Matsui, S M, Offit, P A, Vo, P T, Mackow, E R, Benfield, D A, Shaw, R D, ...
A murine model was used to determine whether neutralizing monoclonal antibodies (MAbs) with heterotypic specificity directed to VP7 (MAb 57-8) or to the VP8 fragment of VP4 (MAb M14) passively...
Mackow, E R, Shaw, R D, Matsui, S M, Vo, P T, Dang, M N, Greenberg, H B
The complete gene 4 nucleotide sequence was determined for rhesus rotavirus and each of 11 viral variants selected by neutralizing monoclonal antibodies. Gene 4 is 2362 bases in length and encodes a...
Leitman, D C, Mackow, E R, Williams, T, Baxter, J D, West, B L
Activators of protein kinase C, such as 12-O-tetradecanoylphorbol 13-acetate (TPA), are known to regulate the expression of many genes, including the tumor necrosis factor alpha (TNF) gene, by...
Mackow, E R, Yamanaka, M Y, Dang, M N, Greenberg, H B
DNA amplification-restricted transcription-translation (DARTT), is based on DNA amplification by the polymerase chain reaction (PCR) and uses PCR to truncate protein-encoding DNA while adding...
Rotavirus diarrhea is caused by nonreplicating viral particles.
Shaw, R D, Hempson, S J, Mackow, E R
Rotaviruses infect the villous epithelium of the small intestine and cause severe diarrhea in young children. The mechanism by which rotavirus causes diarrhea has not been elucidated. It has been...
Hjelle, B, Lee, S W, Song, W, Torrez-Martinez, N, Song, J W, Yanagihara, R, ...
The complete M segment sequences of hantaviruses amplified from tissues of a patient with hantavirus pulmonary syndrome in the northeastern United States and from white-footed mice, Peromyscus...
Identification and baculovirus expression of the VP4 protein of the human group B rotavirus ADRV.
Mackow, E R, Werner-Eckert, R, Fay, M E, Tao, H, Chen, G
A complete cDNA copy of the fourth RNA segment of the human group B rotavirus adult diarrheal rotavirus (ADRV) has been cloned into lambda phage and excised into plasmid pSK Bluescript. Gene segment...
VP4-specific intestinal antibody response to rotavirus in a murine model of heterotypic infection.
Shaw, R D, Groene, W S, Mackow, E R, Merchant, A A, Cheng, E H
We have adapted a murine model of heterotypic rotavirus infection for the purpose of evaluating the intestinal antibody response to an infection that mimics human vaccination. Neonatal mice were...
Mackow, E R, Barnett, J W, Chan, H, Greenberg, H B
Rhesus rotavirus (RRV) gene 4 was cloned into lambda bacteriophage, inserted into a polyhedrin promoter shuttle plasmid, and expressed in Sf9 cells by a recombinant baculovirus. The...
Sequence analysis of gene 11 equivalents from "short" and "super short" strains of rotavirus.
Matsui, S M, Mackow, E R, Matsuno, S, Paul, P S, Greenberg, H B
The molecular basis for the aberrant migration pattern of the gene 11 equivalent in rotaviruses with "short" (human DS-1) and "super short" (human 69M and bovine VMRI) electropherotypes was...
NS35 and not vp7 is the soluble rotavirus protein which binds to target cells.
Bass, D M, Mackow, E R, Greenberg, H B
Recent studies using radiolabeled rotavirus lysates have demonstrated a 35-kilodalton viral protein that binds specifically to the surface of MA104 cells (N. Fukuhara, O. Yoshie, S. Kitakoa, and T....
Mackow, E R, Vo, P T, Broome, R, Bass, D, Greenberg, H B
A baculovirus-expressed VP4 protein derived from the simian rhesus rotavirus (RRV) was used to parenterally immunize murine dams. VP4-immunized dams developed high levels of neutralizing antibodies...
Dharakul, T, Labbe, M, Cohen, J, Bellamy, A R, Street, J E, Mackow, E R, ...
Clearance of chronic murine rotavirus infection in SCID mice can be demonstrated by adoptive transfer of immune CD8+ T lymphocytes from histocompatible donor mice immunized with a murine homotypic...
Serotypic analysis of VP3 and VP7 neutralization escape mutants of rhesus rotavirus.
Shaw, R D, Mackow, E R, Dyall-Smith, M L, Lazdins, I, Holmes, I H, Greenberg, H B
Neutralization escape mutants of simian rotaviruses (rhesus rotavirus and SA11) were tested in hemagglutination inhibition and neutralization assays against hyperimmune and infection sera to...
Serologic analysis of human rotavirus serotypes P1A and P2 by using monoclonal antibodies.
Padilla-Noriega, L, Werner-Eckert, R, Mackow, E R, Gorziglia, M, Larralde, G, Taniguchi, K, ...
Three human rotavirus (HRV) VP4 serotypes and one subtype have been described on the basis of a fourfold or an eightfold-or-greater difference in neutralization titer when tested with hyperimmune...
Padilla-Noriega, L, Fiore, L, Rennels, M B, Losonsky, G A, Mackow, E R, Greenberg, H B
The humoral immune response to rhesus rotavirus (RRV) VP4 and its cleavage products VP5* and VP8* was determined in paired serum samples from 44 infants vaccinated with RRV or human rotavirus-RRV...
Matsui, S M, Offit, P A, Vo, P T, Mackow, E R, Benfield, D A, Shaw, R D, ...
A murine model was used to determine whether neutralizing monoclonal antibodies (MAbs) with heterotypic specificity directed to VP7 (MAb 57-8) or to the VP8 fragment of VP4 (MAb M14) passively...
Mackow, E R, Shaw, R D, Matsui, S M, Vo, P T, Dang, M N, Greenberg, H B
The complete gene 4 nucleotide sequence was determined for rhesus rotavirus and each of 11 viral variants selected by neutralizing monoclonal antibodies. Gene 4 is 2362 bases in length and encodes a...
Leitman, D C, Mackow, E R, Williams, T, Baxter, J D, West, B L
Activators of protein kinase C, such as 12-O-tetradecanoylphorbol 13-acetate (TPA), are known to regulate the expression of many genes, including the tumor necrosis factor alpha (TNF) gene, by...