Lentschat, A., El-Samalouti, V. T., Schletter, J., Kusumoto, S., Brade, L., Rietschel, E. T., ...
The prerequisites for the initiation of pathophysiological effects of endotoxin (lipopolysaccharide [LPS]) include binding to and possibly internalization by target cells. Monocytes/macrophages are...
Schletter, J, Brade, H, Brade, L, Krüger, C, Loppnow, H, Kusumoto, S, ...
Activation of cells by bacterial lipopolysaccharide (LPS) plays a key role in the pathogenesis of gram-negative septic shock. The 55-kDa glycoprotein CD14 is known to bind LPS and initiate cell...
Krziwon, C, Zähringer, U, Kawahara, K, Weidemann, B, Kusumoto, S, Rietschel, E T, ...
Glycosphingolipids (GSL) isolated from the gram-negative lipopolysaccharide (LPS)-free bacterium Sphingomonas paucimobilis have remarkable structural similarities with LPS and its hydrophobic part,...
Specific binding of soluble peptidoglycan and muramyldipeptide to CD14 on human monocytes.
Weidemann, B, Schletter, J, Dziarski, R, Kusumoto, S, Stelter, F, Rietschel, E T, ...
Previously, we were able to show that soluble peptidoglycan (sPG)-induced monokine production in human peripheral monocytes is inhibited by anti-CD14 monoclonal antibodies and by lipid A partial...
Brade, L, Engel, R, Christ, W J, Rietschel, E T
Lipid A monoclonal antibodies, which require for binding the presence of the bisphosphorylated D-glucosamine disaccharide lipid A backbone, were tested against synthetic lipid A precursor Ia and...
Bailat, S, Heumann, D, Le Roy, D, Baumgartner, J D, Rietschel, E T, Glauser, M P, ...
We have previously described cross-reactive antilipopolysaccharide (anti-LPS), or anti-endotoxin, monoclonal antibodies (MAbs) which provide cross-protection in several systems of endotoxin...
Structural characterization of the lipid A component of pathogenic Neisseria meningitidis.
Kulshin, V A, Zähringer, U, Lindner, B, Frasch, C E, Tsai, C M, Dmitriev, B A, ...
The lipid A component of meningococcal lipopolysaccharide was structurally characterized by using chemical modification methods, methylation analysis, 31P nuclear magnetic resonance, and laser...
Moran, A P, Rietschel, E T, Kosunen, T U, Zähringer, U
Lipopolysaccharides (LPS) of four nonencapsulated strains of the human enteric pathogen Campylobacter jejuni were chemically characterized. When applied to two of the strains, extraction by a...
Lipopolysaccharide nomenclature--past, present, and future.
Hitchcock, P J, Leive, L, Mäkelä, P H, Rietschel, E T, Strittmatter, W, Morrison, D C
Labischinski, H, Barnickel, G, Bradaczek, H, Naumann, D, Rietschel, E T, Giesbrecht, P
The conformational properties of the isolated S form of Salmonella sp. lipopolysaccharide (LPS), of Re mutant LPS, and of free lipid A were investigated by using X-ray diffraction and conformational...
Chemical structure of the lipid A component of lipopolysaccharides from Fusobacterium nucleatum.
Hase, S, Hofstad, T, Rietschel, E T
The lipid A component of lipopolysaccharides from Fusobacterium nucleatum Fev 1 consists of beta-1',6-linked D-glucosamine disaccharides, which carry two phosphate groups: one in glycosidic and one...
Rietschel, E T, Lüderitz, O, Volk, W A
The fatty acids present in lipopolysaccharides from Xanthomonas sinensis were identified as decanoic, 9-methyl-decanoic, 2-hydroxy-9-methyl-decanoic, 2-hydroxy-9-methyl-decanoic,...
Kuhn, H M, Brade, L, Appelmelk, B J, Kusumoto, S, Rietschel, E T, Brade, H
A series of monoclonal antibodies directed against lipid A was characterized by using synthetic lipid A analogs and partial structures. These compounds vary in phosphate substitution, acylation...
Ulmer, A J, Heine, H, Feist, W, Kusumoto, S, Kusama, T, Brade, H, ...
We investigated the biological activity of four new synthetic analogs of lipid A, termed PE-1, PE-2, PE-3, and PE-4. All compounds contain an alpha-oxyethyl-linked (-O-CH2-CH2-) phosphoryl group in...
Ulmer, A J, Feist, W, Heine, H, Kirikae, T, Kirikae, F, Kusumoto, S, ...
We have previously shown that the synthetic tetraacyl precursor Ia (compound 406, LA-14-PP, or lipid IVa) was not able to induce the production of tumor necrosis factor, interleukin-1, and...
Tanamoto, K, Shade, U, Rietschel, E T, Kusumuto, S, Shiba, T
The ability of synthetic lipid A analogs to induce prostaglandin synthesis in macrophages was compared with that of native lipopolysaccharide. The synthetic preparations comprised monomeric or...
Wang, M H, Flad, H D, Feist, W, Brade, H, Kusumoto, S, Rietschel, E T, ...
The effect of two synthetic lipid A partial structures, compound 406 (or LA-14-PP, identical in structure to the lipid A precursor, known as Ia or IVa) and compound 401 (lipid X), on the in vitro...
Brade, L, Brandenburg, K, Kuhn, H M, Kusumoto, S, Macher, I, Rietschel, E T, ...
We investigated the immunogenicity and antigenicity of synthetic lipid A and partial structures thereof. Included in the study were compounds which varied in the position of phosphate (1-mono-,...
Immunogenicity and antigenicity of synthetic Escherichia coli lipid A.
Brade, L, Rietschel, E T, Kusumoto, S, Shiba, T, Brade, H
The immunogenicity and antigenicity of synthetic Escherichia coli lipid A (compound 506) and its 1- and 4'-monophosphorylated derivatives (compounds 505 and 504, respectively) and nonphosphorylated...
Chemical characterization of Chlamydia trachomatis lipopolysaccharide.
Nurminen, M, Rietschel, E T, Brade, H
The group-specific antigen of Chlamydia trachomatis serotype L2 was chemically analyzed. It is composed of typical lipopolysaccharide (LPS) components, i.e., D-glucosamine, long-chain 3-hydroxy fatty...
Tanamoto, K, Zähringer, U, McKenzie, G R, Galanos, C, Rietschel, E T, Lüderitz, O, ...
Chemically synthesized lipid A analogs were investigated for several endotoxic activities, including pyrogenicity, lethal toxicity, anticomplement activity, and the capacity to gelate Limulus...
Wollenweber, H W, Rietschel, E T, Hofstad, T, Weintraub, A, Lindberg, A A
The main fatty acids present in lipopolysaccharides from Bacteroides fragilis NCTC 9343 were identified as 13-methyl-tetradecanoic, D-3-hydroxypentadecanoic, D-3-hydroxyhexadecanoic,...
Weidemann, B, Brade, H, Rietschel, E T, Dziarski, R, Bazil, V, Kusumoto, S, ...
We have investigated the interaction of soluble peptidoglycan (sPG), in comparison with lipopolysaccharide (LPS), with human mononuclear cells (MNC) by determining the capacity of sPG to induce...
Lipid A-induced tolerance and hyperreactivity to hypothermia in mice.
Mice responded to lipopolysaccharide (LPS) with a dose-dependent, monophasic hypothermia reaching a maximum at 2 h postinjection. Degraded polysaccharide was not active; free lipid A, however,...
Biological Activities of Lipopolysaccharides and Lipid A from Rhodospirillaceae
Galanos, C., Roppel, J., Weckesser, J., Rietschel, E. T., Mayer, H.
The lipopolysaccharides and free lipid A from several strains of Rhodospirillaceae were assayed comparatively with those of Enterobacteriaceae in a number of biological tests. Free lipid A's from...
The antiendotoxic activity of lipid A antiserum was studied in rabbits, using lipid A (lipopolysaccharide)-induced fever and skin necrosis as test systems. It was found that lipid A antiserum had no...
Mice pretreated (day 0) by a single injection of lipopolysaccharide (LPS) responded with hypothermic tolerance to (LPS) challenge on day 1 and with hypothermic hyperreactivity to LPS challenge on day...
Rietschel, E. T., Kim, Y. B., Watson, D. W., Galanos, C., Lüderitz, O., Westphal, O.
The lipid A component of bacterial lipopolysaccharides (endotoxins), when complexed to bovine serum albumin (BSA) or human serum albumin (HSA), was shown to be a potent pyrogen. Furthermore, rabbits...
Tertiary Structure of Staphylococcus aureus Cell Wall Murein†
Dmitriev, Boris A., Toukach, Filip V., Holst, O., Rietschel, E. T., Ehlers, S.
The recently described scaffold model of murein architecture depicts the gram-negative bacterial cell wall as a gel-like matrix composed of cross-linked glycan strands oriented perpendicularly to the...
Lentschat, A., El-Samalouti, V. T., Schletter, J., Kusumoto, S., Brade, L., Rietschel, E. T., ...
The prerequisites for the initiation of pathophysiological effects of endotoxin (lipopolysaccharide [LPS]) include binding to and possibly internalization by target cells. Monocytes/macrophages are...
Schletter, J, Brade, H, Brade, L, Krüger, C, Loppnow, H, Kusumoto, S, ...
Activation of cells by bacterial lipopolysaccharide (LPS) plays a key role in the pathogenesis of gram-negative septic shock. The 55-kDa glycoprotein CD14 is known to bind LPS and initiate cell...
Krziwon, C, Zähringer, U, Kawahara, K, Weidemann, B, Kusumoto, S, Rietschel, E T, ...
Glycosphingolipids (GSL) isolated from the gram-negative lipopolysaccharide (LPS)-free bacterium Sphingomonas paucimobilis have remarkable structural similarities with LPS and its hydrophobic part,...
Specific binding of soluble peptidoglycan and muramyldipeptide to CD14 on human monocytes.
Weidemann, B, Schletter, J, Dziarski, R, Kusumoto, S, Stelter, F, Rietschel, E T, ...
Previously, we were able to show that soluble peptidoglycan (sPG)-induced monokine production in human peripheral monocytes is inhibited by anti-CD14 monoclonal antibodies and by lipid A partial...
Brade, L, Engel, R, Christ, W J, Rietschel, E T
Lipid A monoclonal antibodies, which require for binding the presence of the bisphosphorylated D-glucosamine disaccharide lipid A backbone, were tested against synthetic lipid A precursor Ia and...
Bailat, S, Heumann, D, Le Roy, D, Baumgartner, J D, Rietschel, E T, Glauser, M P, ...
We have previously described cross-reactive antilipopolysaccharide (anti-LPS), or anti-endotoxin, monoclonal antibodies (MAbs) which provide cross-protection in several systems of endotoxin...
Structural characterization of the lipid A component of pathogenic Neisseria meningitidis.
Kulshin, V A, Zähringer, U, Lindner, B, Frasch, C E, Tsai, C M, Dmitriev, B A, ...
The lipid A component of meningococcal lipopolysaccharide was structurally characterized by using chemical modification methods, methylation analysis, 31P nuclear magnetic resonance, and laser...
Moran, A P, Rietschel, E T, Kosunen, T U, Zähringer, U
Lipopolysaccharides (LPS) of four nonencapsulated strains of the human enteric pathogen Campylobacter jejuni were chemically characterized. When applied to two of the strains, extraction by a...
Lipopolysaccharide nomenclature--past, present, and future.
Hitchcock, P J, Leive, L, Mäkelä, P H, Rietschel, E T, Strittmatter, W, Morrison, D C
Labischinski, H, Barnickel, G, Bradaczek, H, Naumann, D, Rietschel, E T, Giesbrecht, P
The conformational properties of the isolated S form of Salmonella sp. lipopolysaccharide (LPS), of Re mutant LPS, and of free lipid A were investigated by using X-ray diffraction and conformational...
Chemical structure of the lipid A component of lipopolysaccharides from Fusobacterium nucleatum.
Hase, S, Hofstad, T, Rietschel, E T
The lipid A component of lipopolysaccharides from Fusobacterium nucleatum Fev 1 consists of beta-1',6-linked D-glucosamine disaccharides, which carry two phosphate groups: one in glycosidic and one...
Rietschel, E T, Lüderitz, O, Volk, W A
The fatty acids present in lipopolysaccharides from Xanthomonas sinensis were identified as decanoic, 9-methyl-decanoic, 2-hydroxy-9-methyl-decanoic, 2-hydroxy-9-methyl-decanoic,...
Kuhn, H M, Brade, L, Appelmelk, B J, Kusumoto, S, Rietschel, E T, Brade, H
A series of monoclonal antibodies directed against lipid A was characterized by using synthetic lipid A analogs and partial structures. These compounds vary in phosphate substitution, acylation...
Ulmer, A J, Heine, H, Feist, W, Kusumoto, S, Kusama, T, Brade, H, ...
We investigated the biological activity of four new synthetic analogs of lipid A, termed PE-1, PE-2, PE-3, and PE-4. All compounds contain an alpha-oxyethyl-linked (-O-CH2-CH2-) phosphoryl group in...
Ulmer, A J, Feist, W, Heine, H, Kirikae, T, Kirikae, F, Kusumoto, S, ...
We have previously shown that the synthetic tetraacyl precursor Ia (compound 406, LA-14-PP, or lipid IVa) was not able to induce the production of tumor necrosis factor, interleukin-1, and...
Tanamoto, K, Shade, U, Rietschel, E T, Kusumuto, S, Shiba, T
The ability of synthetic lipid A analogs to induce prostaglandin synthesis in macrophages was compared with that of native lipopolysaccharide. The synthetic preparations comprised monomeric or...
Wang, M H, Flad, H D, Feist, W, Brade, H, Kusumoto, S, Rietschel, E T, ...
The effect of two synthetic lipid A partial structures, compound 406 (or LA-14-PP, identical in structure to the lipid A precursor, known as Ia or IVa) and compound 401 (lipid X), on the in vitro...
Brade, L, Brandenburg, K, Kuhn, H M, Kusumoto, S, Macher, I, Rietschel, E T, ...
We investigated the immunogenicity and antigenicity of synthetic lipid A and partial structures thereof. Included in the study were compounds which varied in the position of phosphate (1-mono-,...
Immunogenicity and antigenicity of synthetic Escherichia coli lipid A.
Brade, L, Rietschel, E T, Kusumoto, S, Shiba, T, Brade, H
The immunogenicity and antigenicity of synthetic Escherichia coli lipid A (compound 506) and its 1- and 4'-monophosphorylated derivatives (compounds 505 and 504, respectively) and nonphosphorylated...
Chemical characterization of Chlamydia trachomatis lipopolysaccharide.
Nurminen, M, Rietschel, E T, Brade, H
The group-specific antigen of Chlamydia trachomatis serotype L2 was chemically analyzed. It is composed of typical lipopolysaccharide (LPS) components, i.e., D-glucosamine, long-chain 3-hydroxy fatty...
Tanamoto, K, Zähringer, U, McKenzie, G R, Galanos, C, Rietschel, E T, Lüderitz, O, ...
Chemically synthesized lipid A analogs were investigated for several endotoxic activities, including pyrogenicity, lethal toxicity, anticomplement activity, and the capacity to gelate Limulus...
Wollenweber, H W, Rietschel, E T, Hofstad, T, Weintraub, A, Lindberg, A A
The main fatty acids present in lipopolysaccharides from Bacteroides fragilis NCTC 9343 were identified as 13-methyl-tetradecanoic, D-3-hydroxypentadecanoic, D-3-hydroxyhexadecanoic,...
Weidemann, B, Brade, H, Rietschel, E T, Dziarski, R, Bazil, V, Kusumoto, S, ...
We have investigated the interaction of soluble peptidoglycan (sPG), in comparison with lipopolysaccharide (LPS), with human mononuclear cells (MNC) by determining the capacity of sPG to induce...
Lipid A-induced tolerance and hyperreactivity to hypothermia in mice.
Mice responded to lipopolysaccharide (LPS) with a dose-dependent, monophasic hypothermia reaching a maximum at 2 h postinjection. Degraded polysaccharide was not active; free lipid A, however,...
Biological Activities of Lipopolysaccharides and Lipid A from Rhodospirillaceae
Galanos, C., Roppel, J., Weckesser, J., Rietschel, E. T., Mayer, H.
The lipopolysaccharides and free lipid A from several strains of Rhodospirillaceae were assayed comparatively with those of Enterobacteriaceae in a number of biological tests. Free lipid A's from...
The antiendotoxic activity of lipid A antiserum was studied in rabbits, using lipid A (lipopolysaccharide)-induced fever and skin necrosis as test systems. It was found that lipid A antiserum had no...
Mice pretreated (day 0) by a single injection of lipopolysaccharide (LPS) responded with hypothermic tolerance to (LPS) challenge on day 1 and with hypothermic hyperreactivity to LPS challenge on day...
Rietschel, E. T., Kim, Y. B., Watson, D. W., Galanos, C., Lüderitz, O., Westphal, O.
The lipid A component of bacterial lipopolysaccharides (endotoxins), when complexed to bovine serum albumin (BSA) or human serum albumin (HSA), was shown to be a potent pyrogen. Furthermore, rabbits...
Tertiary Structure of Staphylococcus aureus Cell Wall Murein†
Dmitriev, Boris A., Toukach, Filip V., Holst, O., Rietschel, E. T., Ehlers, S.
The recently described scaffold model of murein architecture depicts the gram-negative bacterial cell wall as a gel-like matrix composed of cross-linked glycan strands oriented perpendicularly to the...
Analysis of bypass activation of C3 by endotoxic LPS and loss of this potency
Dierich, M. P., Bitter-Suermann, D., König, W., Hadding, U., Galanos, C., Rietschel, E. T.
Endotoxic lipopolysaccharides prepared from Salmonella minnesota smooth form (LPS-S) and rough form, R 595 (LPS-R) activate C3 in guinea-pig serum as is demonstrated by C3 kinetics and generation of...