Mammalian cell size is controlled by mTOR and its downstream targets S6K1 and 4EBP1/eIF4E (2002)
Fingar, Diane C., Salama, Sofie, Tsou, Christina, Harlow, Ed, Blenis, John
Zhao, Jiyong, Kennedy, Brian K., Lawrence, Brandon D., Barbie, David A., Matera, A. Gregory, Fletcher, Jonathan A., ...
In eukaryotic cells, histone gene expression is one of the major events that mark entry into S phase. While this process is tightly linked to cell cycle position, how it is regulated by the cell...
Nuclear organization of DNA replication in primary mammalian cells (2000)
Kennedy, Brian K., Barbie, David A., Classon, Marie, Dyson, Nicholas, Harlow, Ed
Zhao, Jiyong, Kennedy, Brian K., Lawrence, Brandon D., Barbie, David A., Matera, A. Gregory, Fletcher, Jonathan A., ...
Expression of NPAT, a novel substrate of cyclin E-CDK2, promotes S-phase entry (1998)
Zhao, Jiyong, Dynlacht, Brian, Imai, Takashi, Hori, Tada-aki, Harlow, Ed
Antibodies : a laboratory manual / Ed. Harlow, David Lane. [editors] (1988)
Incluye bibliografía e índice
Antibodies : a laboratory manual / Ed Harlow, David Lane (1988)
Incluye bibliografía e índice
Mitogen-activated protein kinase-mediated Fas apoptotic signaling pathway
Goillot, Evelyne, Raingeaud, Joel, Ranger, Ann, Tepper, Robert I., Davis, Roger. J., Harlow, Ed, ...
Ligation of the cell surface receptor Fas/APO-1 (CD95) by its specific ligand or by anti-Fas antibodies rapidly induces apoptosis in susceptible cells. To characterize the molecular events involved...
pRB plays an essential role in cell cycle arrest induced by DNA damage
Harrington, Elizabeth A., Bruce, Jacqueline L., Harlow, Ed, Dyson, Nicholas
To maintain genome stability, cells with damaged DNA must arrest to allow repair of mutations before replication. Although several key components required to elicit this arrest have been discovered,...
Combinatorial roles for pRB, p107, and p130 in E2F-mediated cell cycle control
Classon, Marie, Salama, Sofie, Gorka, Carolyn, Mulloy, Roseann, Braun, Pascal, Harlow, Ed
Numerous studies have implicated the pRB family of nuclear proteins in the control of cell cycle progression. Although over-expression experiments have revealed that each of these proteins, pRB,...
Opposing roles of pRB and p107 in adipocyte differentiation
Classon, Marie, Kennedy, Brian K., Mulloy, Roseann, Harlow, Ed
The retinoblastoma (RB) family of proteins, pRB, p107, and p130, have been postulated to be partially redundant in their ability to regulate progression through the G1 phase of the cell cycle....
Substrate recruitment to cyclin-dependent kinase 2 by a multipurpose docking site on cyclin A
Schulman, Brenda A., Lindstrom, Derek L., Harlow, Ed
An important question in the cell cycle field is how cyclin-dependent kinases (cdks) target their substrates. We have studied the role of a conserved hydrophobic patch on the surface of cyclin A in...
Stevenson, Lauren F., Kennedy, Brian K., Harlow, Ed
We have undertaken an extensive screen to identify Saccharomyces cerevisiae genes whose products are involved in cell cycle progression. We report the identification of 113 genes, including 19...
Kennedy, Brian K., Liu, Oliver W., Dick, Fred A., Dyson, Nick, Harlow, Ed, Vidal, Marc
We have developed a yeast model system to address transcriptional repression by the retinoblastoma protein (pRB). When fused to the DNA-binding domain of Gal4p (DB-pRB), pRB can repress transcription...
McCaffrey, Jennifer, Yamasaki, Lili, Dyson, Nicholas J., Harlow, Ed, Griep, Anne E.
Complexes between the retinoblastoma protein (pRb) and the transcription factor E2F-1 are thought to be important for regulating cell proliferation. We have shown previously that the E7 oncoprotein...
Lai, Albert, Kennedy, Brian K., Barbie, David A., Bertos, Nicholas R., Yang, Xiang Jiao, Theberge, Marie-Christine, ...
Retinoblastoma (RB) tumor suppressor family pocket proteins induce cell cycle arrest by repressing transcription of E2F-regulated genes through both histone deacetylase (HDAC)-dependent and...
Proteome-scale purification of human proteins from bacteria
Braun, Pascal, Hu, Yanhui, Shen, Binghua, Halleck, Allison, Koundinya, Malvika, Harlow, Ed, ...
The completion of the human genome project and the development of high-throughput approaches herald a dramatic acceleration in the pace of biological research. One of the most compelling next steps...
Mammalian cell size is controlled by mTOR and its downstream targets S6K1 and 4EBP1/eIF4E
Fingar, Diane C., Salama, Sofie, Tsou, Christina, Harlow, Ed, Blenis, John
The coordinated action of cell cycle progression and cell growth (an increase in cell size and cell mass) is critical for sustained cellular proliferation, yet the biochemical signals that control...
A uniform nomenclature has been agreed upon for monoclonal antibodies directed against virus-coded proteins of simian virus 40 and polyoma virus. Blocks of numbers from PAb1 to PAb999 have been...
Expression of NPAT, a novel substrate of cyclin E–CDK2, promotes S-phase entry
Zhao, Jiyong, Dynlacht, Brian, Imai, Takashi, Hori, Tada-aki, Harlow, Ed
To understand the mechanisms by which CDKs regulate cell cycle progression, it is necessary to identify and characterize the physiological substrates of these kinases. We have developed a screening...
NPAT links cyclin E–Cdk2 to the regulation of replication-dependent histone gene transcription
Zhao, Jiyong, Kennedy, Brian K., Lawrence, Brandon D., Barbie, David A., Matera, A. Gregory, Fletcher, Jonathan A., ...
In eukaryotic cells, histone gene expression is one of the major events that mark entry into S phase. While this process is tightly linked to cell cycle position, how it is regulated by the cell...
Nuclear organization of DNA replication in primary mammalian cells
Kennedy, Brian K., Barbie, David A., Classon, Marie, Dyson, Nicholas, Harlow, Ed
Using methods that conserve nuclear architecture, we have reanalyzed the spatial organization of the initiation of mammalian DNA synthesis. Contrary to the commonly held view that replication begins...
Nuclear Reorganization of Mammalian DNA Synthesis Prior to Cell Cycle Exit
Barbie, David A., Kudlow, Brian A., Frock, Richard, Zhao, Jiyong, Johnson, Brett R., Dyson, Nicholas, ...
In primary mammalian cells, DNA replication initiates in a small number of perinucleolar, lamin A/C-associated foci. During S-phase progression in proliferating cells, replication foci distribute to...
Johnson, Brett R., Nitta, Ryan T., Frock, Richard L., Mounkes, Leslie, Barbie, David A., Stewart, Colin L., ...
The retinoblastoma protein (pRB) is a critical regulator of cell proliferation and differentiation and an important tumor suppressor. In the G1 phase of the cell cycle, pRB localizes to perinucleolar...
Park, Jaehong, Hu, Yanhui, Murthy, T. V. S., Vannberg, Fredrik, Shen, Binghua, Rolfs, Andreas, ...
Kinases catalyze the phosphorylation of proteins, lipids, sugars, nucleosides, and other important cellular metabolites and play key regulatory roles in all aspects of eukaryotic cell physiology....
Mitogen-activated protein kinase-mediated Fas apoptotic signaling pathway
Goillot, Evelyne, Raingeaud, Joel, Ranger, Ann, Tepper, Robert I., Davis, Roger. J., Harlow, Ed, ...
Ligation of the cell surface receptor Fas/APO-1 (CD95) by its specific ligand or by anti-Fas antibodies rapidly induces apoptosis in susceptible cells. To characterize the molecular events involved...
pRB plays an essential role in cell cycle arrest induced by DNA damage
Harrington, Elizabeth A., Bruce, Jacqueline L., Harlow, Ed, Dyson, Nicholas
To maintain genome stability, cells with damaged DNA must arrest to allow repair of mutations before replication. Although several key components required to elicit this arrest have been discovered,...
Combinatorial roles for pRB, p107, and p130 in E2F-mediated cell cycle control
Classon, Marie, Salama, Sofie, Gorka, Carolyn, Mulloy, Roseann, Braun, Pascal, Harlow, Ed
Numerous studies have implicated the pRB family of nuclear proteins in the control of cell cycle progression. Although over-expression experiments have revealed that each of these proteins, pRB,...
Opposing roles of pRB and p107 in adipocyte differentiation
Classon, Marie, Kennedy, Brian K., Mulloy, Roseann, Harlow, Ed
The retinoblastoma (RB) family of proteins, pRB, p107, and p130, have been postulated to be partially redundant in their ability to regulate progression through the G1 phase of the cell cycle....
Substrate recruitment to cyclin-dependent kinase 2 by a multipurpose docking site on cyclin A
Schulman, Brenda A., Lindstrom, Derek L., Harlow, Ed
An important question in the cell cycle field is how cyclin-dependent kinases (cdks) target their substrates. We have studied the role of a conserved hydrophobic patch on the surface of cyclin A in...
Stevenson, Lauren F., Kennedy, Brian K., Harlow, Ed
We have undertaken an extensive screen to identify Saccharomyces cerevisiae genes whose products are involved in cell cycle progression. We report the identification of 113 genes, including 19...
Kennedy, Brian K., Liu, Oliver W., Dick, Fred A., Dyson, Nick, Harlow, Ed, Vidal, Marc
We have developed a yeast model system to address transcriptional repression by the retinoblastoma protein (pRB). When fused to the DNA-binding domain of Gal4p (DB-pRB), pRB can repress transcription...
McCaffrey, Jennifer, Yamasaki, Lili, Dyson, Nicholas J., Harlow, Ed, Griep, Anne E.
Complexes between the retinoblastoma protein (pRb) and the transcription factor E2F-1 are thought to be important for regulating cell proliferation. We have shown previously that the E7 oncoprotein...
Lai, Albert, Kennedy, Brian K., Barbie, David A., Bertos, Nicholas R., Yang, Xiang Jiao, Theberge, Marie-Christine, ...
Retinoblastoma (RB) tumor suppressor family pocket proteins induce cell cycle arrest by repressing transcription of E2F-regulated genes through both histone deacetylase (HDAC)-dependent and...
Proteome-scale purification of human proteins from bacteria
Braun, Pascal, Hu, Yanhui, Shen, Binghua, Halleck, Allison, Koundinya, Malvika, Harlow, Ed, ...
The completion of the human genome project and the development of high-throughput approaches herald a dramatic acceleration in the pace of biological research. One of the most compelling next steps...
Mammalian cell size is controlled by mTOR and its downstream targets S6K1 and 4EBP1/eIF4E
Fingar, Diane C., Salama, Sofie, Tsou, Christina, Harlow, Ed, Blenis, John
The coordinated action of cell cycle progression and cell growth (an increase in cell size and cell mass) is critical for sustained cellular proliferation, yet the biochemical signals that control...
A uniform nomenclature has been agreed upon for monoclonal antibodies directed against virus-coded proteins of simian virus 40 and polyoma virus. Blocks of numbers from PAb1 to PAb999 have been...
Expression of NPAT, a novel substrate of cyclin E–CDK2, promotes S-phase entry
Zhao, Jiyong, Dynlacht, Brian, Imai, Takashi, Hori, Tada-aki, Harlow, Ed
To understand the mechanisms by which CDKs regulate cell cycle progression, it is necessary to identify and characterize the physiological substrates of these kinases. We have developed a screening...
NPAT links cyclin E–Cdk2 to the regulation of replication-dependent histone gene transcription
Zhao, Jiyong, Kennedy, Brian K., Lawrence, Brandon D., Barbie, David A., Matera, A. Gregory, Fletcher, Jonathan A., ...
In eukaryotic cells, histone gene expression is one of the major events that mark entry into S phase. While this process is tightly linked to cell cycle position, how it is regulated by the cell...
Nuclear organization of DNA replication in primary mammalian cells
Kennedy, Brian K., Barbie, David A., Classon, Marie, Dyson, Nicholas, Harlow, Ed
Using methods that conserve nuclear architecture, we have reanalyzed the spatial organization of the initiation of mammalian DNA synthesis. Contrary to the commonly held view that replication begins...
Nuclear Reorganization of Mammalian DNA Synthesis Prior to Cell Cycle Exit
Barbie, David A., Kudlow, Brian A., Frock, Richard, Zhao, Jiyong, Johnson, Brett R., Dyson, Nicholas, ...
In primary mammalian cells, DNA replication initiates in a small number of perinucleolar, lamin A/C-associated foci. During S-phase progression in proliferating cells, replication foci distribute to...
Johnson, Brett R., Nitta, Ryan T., Frock, Richard L., Mounkes, Leslie, Barbie, David A., Stewart, Colin L., ...
The retinoblastoma protein (pRB) is a critical regulator of cell proliferation and differentiation and an important tumor suppressor. In the G1 phase of the cell cycle, pRB localizes to perinucleolar...
Park, Jaehong, Hu, Yanhui, Murthy, T. V. S., Vannberg, Fredrik, Shen, Binghua, Rolfs, Andreas, ...
Kinases catalyze the phosphorylation of proteins, lipids, sugars, nucleosides, and other important cellular metabolites and play key regulatory roles in all aspects of eukaryotic cell physiology....
Kinase requirements in human cells: I. Comparing kinase requirements across various cell types
Grueneberg, Dorre A., Degot, Sebastien, Pearlberg, Joseph, Li, Wenliang, Davies, Joan E., Baldwin, Amy, ...
shRNA loss-of-function screens were used to identify kinases that were rate-limiting for promoting cell proliferation and survival. Here, we study the differences in kinase requirements among various...
Baldwin, Amy, Li, Wenliang, Grace, Miranda, Pearlberg, Joseph, Harlow, Ed, Münger, Karl, ...
Human papillomavirus (HPV) oncoproteins subvert cellular signaling pathways, including kinase pathways, during the carcinogenic process. To identify kinases targeted by the HPV16 E7 oncoprotein,...
Bommi-Reddy, Archana, Almeciga, Ingrid, Sawyer, Jacqueline, Geisen, Christoph, Li, Wenliang, Harlow, Ed, ...
Clear cell renal carcinomas are the most common form of kidney cancer and frequently are linked to biallelic inactivation of the von Hippel-Lindau (VHL) tumor suppressor gene. The VHL gene product,...
Grueneberg, Dorre A., Li, Wenliang, Davies, Joan E., Sawyer, Jacqueline, Pearlberg, Joseph, Harlow, Ed
Functional differences among human cells have been difficult to identify by standard biochemical methods. Loss-of-function shRNA screens provide an unbiased method to compare protein requirements...